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Electron spin resonance studies of spin-labeled mammalian cells by detection of surface-membrane signals.

Abstract：

:Lipid-soluble spin labels were incorporated into human lymphocytes and mouse L-cells and the resulting electron spin resonance spectra were compared with spectra obtained from similarly labeled human erythrocytes. Spin labels were found in all subcellular fractions of the nucleated cells that contained membranes. Spinlabeled cells remained viable and capable of replicating in vitro. Electron spin resonance signals from spin-labeled nucleated cells underwent a time- and temperature-dependent decay that was reversed by bathing the cells in K(3)Fe(CN)(6). The demonstration of a relative cell impermeability to ferricyanide, as measured by both colorimetric and radioisotopic label methods, indicated that only spin-labeled molecules in the surface membrane were reactivated when ferricyanide was added to spin-labeled cells after the electron spin resonance signal had decayed.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Kaplan J,Canonico PG,Caspary WJ

doi

10.1073/pnas.70.1.66

subject

Has Abstract

pub_date

1973-01-01 00:00:00

pages

66-70

issue

1

eissn

0027-8424

issn

1091-6490

journal_volume

70

pub_type

杂志文章

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