Abstract:
:Poly(A)-containing RNA was isolated from ovaries of Xenopus laevis laevis and Triturus cristatus carnifex and used as a template for the synthesis of radioactive complementary DNA with RNA-dependent DNA polymerase. When annealed with an excess of homologous DNA, the complementary DNA is rendered double-stranded with kinetics that suggest that the coding sequences are single-copy in both these organisms. In Triturus, these sequences are distinct from the majority of the genome, which consists of repeated sequences, and distinct from the ribosomal cistrons, which are present in proportion to the increase in C-value relative to the Xenopus genome. Moreover, the number of different poly(A)-containing molecules in the ovary (sequence complexity) is the same in Xenopus and in Triturus.
journal_name
Proc Natl Acad Sci U S Aauthors
Rosbash M,Ford PJ,Bishop JOdoi
10.1073/pnas.71.9.3746subject
Has Abstractpub_date
1974-09-01 00:00:00pages
3746-50issue
9eissn
0027-8424issn
1091-6490journal_volume
71pub_type
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