Abstract:
:Astrocytes in primary cultures constitute an exceedingly useful preparation for studies of astroglial development and function. These cells, however, demonstrate a pronounced plasticity in their reactions to culturing conditions. Thus, species and spatiotemporal region of CNS chosen for source of cells, dissociation procedures used, cell density in culture, culture medium chosen, type and/or concentration of serum used (if any) and exposure to dibutyryl cyclic AMP (dBcAMP) may all markedly affect the epigenotype of the cells. This provides an experimental tool for studies of astroglial development and function, but is at the same time a reason for caution in the interpretation of data when such cultured cells are used as models of their in vivo counterparts. About 95% of the cells in cultures used by the authors are positive for astrocyte-specific markers (glial fibrillary acidic protein and glutamine synthetase), and the cells possess presumably astrocytic characteristics such as high potassium permeability. This latter characteristic may be drastically altered by minor changes in culturing conditions. It is likely that an overwhelming majority of the astrocytes in such cultures are protoplasmic in nature. Addition of dBcAMP to the culture medium results in a pronounced morphological and a more modest functional differentiation.
journal_name
Dev Neuroscijournal_title
Developmental neuroscienceauthors
Juurlink BH,Hertz Ldoi
10.1159/000112295subject
Has Abstractpub_date
1985-01-01 00:00:00pages
263-77issue
5-6eissn
0378-5866issn
1421-9859journal_volume
7pub_type
杂志文章,评审abstract::It has been hypothesized that changes in striatal-mediated dopamine modulation during adolescence may increase the risk for initiating substance abuse as a result of its fundamental role in arbitrating reward sensitivity and motivation during learning and decision making. However, substance abuse during adolescence ma...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000337724
更新日期:2012-01-01 00:00:00
abstract::Dissociated cells of cerebral hemispheres from 8-day-old chick embryos were cultivated for 8 days in polylysine-coated Petri dishes. Changes of DNA, RNA, total proteins, putrescine, spermidine, spermine, free amino acids and enzymes involved in polyamine and GABA metabolism were studied throughout neuronal development...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112755
更新日期:1981-01-01 00:00:00
abstract::In the presence of nerve growth factor (NGF) PC12 pheochromocytoma cells develop properties of sympathetic neurons and extend long microtubule-containing neurites. PC12 cell microtubule protein was isolated using an assembly and disassembly procedure, either directly from an unlabeled cell extract or by copolymerizati...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112330
更新日期:1983-01-01 00:00:00
abstract::Developmental expression of neutral monoglycosylceramides (MGCs) has been examined in rat brain from embryonic day 15 (E15) to postnatal day 30 (P30) and adulthood. To this point, glucosylceramide (GlcCer) is the only MGC that has been characterized in embryonic brain. Galactosylceramide (GalCer) appears at P1, increa...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111201
更新日期:1997-01-01 00:00:00
abstract::Neuron-specific enolase (NSE) is a unique form of the glycolytic enzyme, enolase, which, in the brain, is strictly localized in differentiated neurons and neuroendocrine cells. In this report evidence is presented that NSE is first seen in the cell bodies of developing chick auditory and vestibular neurons shortly aft...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112689
更新日期:1982-01-01 00:00:00
abstract::The L-type calcium channel activator +/-Bay K 8644 has recently been shown to provoke self-injurious biting in young mice. Since the serotonergic systems have been implicated in the expression of self-injurious behavior in both humans and animals, the present studies tested whether drugs influencing serotonin systems ...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000066747
更新日期:2002-01-01 00:00:00
abstract::Maternal infection during pregnancy increases the risk of neurobehavioral problems in offspring. Evidence from rodent models indicates that the maternal immune response to infection can alter fetal brain development, particularly in the hippocampus. However, information on the effects of maternal viral infection on fe...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000486850
更新日期:2018-01-01 00:00:00
abstract::Traumatic brain injury (TBI) is predominantly a clinical problem of young persons, resulting in chronic cognitive and behavioral deficits. Specifically, the physiological response to a diffuse biomechanical injury in a maturing brain can clearly alter normal neuroplasticity. To properly evaluate and investigate develo...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000094163
更新日期:2006-01-01 00:00:00
abstract::Previous studies have demonstrated that exposure to ethanol during development delays the rate at which axons in certain central nervous system tracts acquire myelin. This delay appears to be related to an alteration in oligodendrocyte function and not to an aberrancy in axon size or number. The present study was desi...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112154
更新日期:1991-01-01 00:00:00
abstract::α-Synuclein is one of the most abundant proteins in presynaptic terminals. Normal expression of α-synuclein is essential for neuronal survival and it prevents the initiation of apoptosis in neurons through covalent cross-linking of cytochrome c released from mitochondria. Exocytosis of α-synuclein occurs with neuronal...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000321342
更新日期:2010-01-01 00:00:00
abstract::In vertebrate species, the neuropeptide oxytocin (OT) has been implicated in neural and behavioral development. Although several OT-like peptides have been characterized in invertebrate species, the ontogenesis of the OT-like system has not yet been described in these species. Thus, the aim of the present study was to...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000235942
更新日期:2010-03-01 00:00:00
abstract::Evidence from retroviral marking techniques and immortalized cell lines indicates that multipotential stem cells exist in many areas of the developing central nervous system. However, the factors that influence the commitment of these stem cells into distinct neuronal or glial lineages are not known. We have created a...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111301
更新日期:1995-01-01 00:00:00
abstract::Adult GM2 gangliosidosis is a rare autosomal recessive disease with widely varying neurological and psychiatric manifestations. It is caused by marked deficiency, but not total absence, of beta-hexosaminidase (Hex) A, due to a single base change in the alpha-subunit gene of Hex, resulting in a substitution of Ser for ...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112200
更新日期:1991-01-01 00:00:00
abstract::Neuroprotective cold-shock proteins (CSPs) are abundant in the normothermic neonatal rodent brain but decrease with advancing neurodevelopmental age and are low or absent in the adult brain. It has not been established if neurodevelopmental age alters the baseline expression of CSPs in the human brain. Here, we tested...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000493637
更新日期:2018-01-01 00:00:00
abstract::When cells from the cerebellum of late-gestation rats were grown at low density (50-100 cells/mm2) in a three-dimensional culture system, they rapidly expressed morphological and electrophysiological properties of neurons. Growth and differentiation of the population of solitary neurons as a whole was statistically as...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111645
更新日期:1992-01-01 00:00:00
abstract::Prior studies suggest that non-canonical proteolipid protein (PLP) gene expression occurs during development in non-myelinating neurons as well as myelinating oligodendroglia in mammalian brain. To assess this possibility in neostriatum, a region of uncertain PLP gene expression in neurons, morphological and electroph...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000330321
更新日期:2011-01-01 00:00:00
abstract::The metabolism of [1-13C]D-glucose by astrocytes, neurons and mixed astroglial/neuronal cultures derived from the striatum of fetal rats was studied using NMR. Metabolic activity was studied in resting and depolarized cells (55 mM K+), with dibutyryl cyclic-AMP added to the medium to promote cell differentiation, and ...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111346
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:Neonatal hypoxic-ischemic (HI) encephalopathy occurs in 1-4 per 1,000 live term births and can cause devastating neurodevelopmental disabilities. Currently, therapeutic hypothermia (TH) is the only treatment with proven efficacy. Since TH is associated with decreased cerebral metabolism and cerebral blood fl...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000381704
更新日期:2015-01-01 00:00:00
abstract::We have previously provided evidence that ferritin binds selectively to white matter tracts in adult mouse and human brains. In cell culture experiments, ferritin binding is specifically localized to oligodendrocytes. The goal of the present study is to test the hypothesis that the developmental pattern for ferritin b...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000065704
更新日期:2002-01-01 00:00:00
abstract::Stromal-derived factor 1 (SDF-1), a known chemoattractant, and its receptor CXCR4 are widely expressed in the developing and adult cerebral cortex. Recent studies have highlighted potential roles for SDF-1 during early cortical development. In view of the current findings, our histological analysis has revealed a dist...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000109857
更新日期:2008-01-01 00:00:00
abstract::Plexin is a type I membrane protein which was identified in Xenopus nervous system by hybridoma technique. Molecular cloning studies demonstrated that the extracellular segment of the plexin protein possesses three internal repeats of cysteine cluster which are homologous to the cysteine-rich domain of the c-met proto...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000111192
更新日期:1997-01-01 00:00:00
abstract::Excitotoxicity plays a key role during insults to the developing brain such as neonatal encephalopathy, stroke, and encephalopathy of prematurity. Such insults affect many thousands of infants each year. Excitotoxicity causes frank lesions due to cell death and gliosis and disturbs normal developmental process, leadin...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000464131
更新日期:2017-01-01 00:00:00
abstract::We have shown that the developmentally regulated appearance of the two myelin-associated glycoprotein (MAG) polypeptides in normal mouse brain myelin does not reflect the developmental pattern of differential splicing of primary gene transcripts as determined earlier by RNase protection assays. Contrary to expectation...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111857
更新日期:1990-01-01 00:00:00
abstract::The current work tested the hypothesis that the zinc status of a cell influences its sensitivity to iron-induced oxidative stress. Human IMR-32 neuroblastoma cells were cultured for 24 h in nonchelated control media (5 microM zinc; 4.5 microM iron), or in media that was treated with DTPA to reduce its zinc content (ch...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000065691
更新日期:2002-01-01 00:00:00
abstract::The neurochemical effect of postnatal exposure to methadone (METH), undernutrition (UND), and methadone and undernutrition (METH-UND) on the whole brain and cerebellum were examined in 21- and 60-day-old rats. At 21 days, body weight reductions of UND and METH-UND rats exceeded that of the METH animals. However, METH ...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112698
更新日期:1982-01-01 00:00:00
abstract::Schizophrenia is a chronic and debilitating mental illness characterized by a broad range of abnormal behaviors, including delusions and hallucinations, impaired cognitive function, as well as mood disturbances and social withdrawal. Due to the heterogeneous nature of the disease, the causes of schizophrenia are very ...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000346157
更新日期:2013-01-01 00:00:00
abstract::The long noncoding RNA MSNP1AS (moesin pseudogene 1, antisense) is a functional element that was previously associated with autism spectrum disorder (ASD) with genome-wide significance. Expression of MSNP1AS was increased 12-fold in the cerebral cortex of individuals with ASD and 22-fold in individuals with a genome-w...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000453258
更新日期:2016-01-01 00:00:00
abstract::Adrenoleukodystrophy (ALD) is an X-linked recessive disorder that affects mainly the nervous system white matter and the adrenal cortex. It is associated with an abnormal accumulation of saturated very long chain fatty acids and can be diagnosed by demonstrating an excess of these substances in plasma or red cells. Ou...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000112170
更新日期:1991-01-01 00:00:00
abstract::We have used whole-cell and single-channel recording techniques to investigate the electrophysiological properties of cultured ovine oligodendrocytes (OLGs). Our studies have led to the following conclusions. (1) Cultured mature OLGs express a variety of voltage-dependent K+ conductances including an outward current t...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000111893
更新日期:1989-01-01 00:00:00
abstract::Primary cultures of cerebellum from 5-day-old rats indicated that proliferating neuroblasts synthesize and release nerve growth factor (NGF). Since NGF promotes DNA synthesis in these cells, our findings demonstrate that the early developing cerebellum is a suitable physiological model for studying the autocrine mitog...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111673
更新日期:1992-01-01 00:00:00