Abstract:
:The antiproliferative effect of 2',5' A3 core and 2',5'-3'dA3 (cordycepin trimer) core was measured in 8 human tumor cell lines. Cells were treated in a dose-response manner for 72 hr and the concentration of drug necessary to inhibit cell growth 50% (GI50) was determined. A wide range of sensitivities to these drugs was found, even among tumors of the same histological type. The cell lines showed different sensitivities and dose-response curves to the 2',5'A3 and 2',5'-3'dA3 cores. Uptake studies of the 2',5'A3 and 2',5'-3'dA3 cores, using high-pressure liquid chromatography, demonstrated that both cores were rapidly degraded in the tissue culture medium and taken up as adenosine or cordycepin, respectively. There was a direct correlation between the uptake of cordycepin and the antiproliferative effect. In contrast, there was no correlation between cell sensitivity and the uptake of the 2',5'A3 core degradation products. Analysis of intracellular nucleosides and nucleotides indicated that differences in intracellular metabolism of adenosine might explain the different sensitivities of the various cell lines to 2',5'A3 core. Molar equivalent concentrations of adenosine and cordycepin inhibited cell growth; however, equimolar concentrations of these nucleosides were not effective. In addition, the antiproliferative effect of both core compounds and their corresponding nucleosides could be potentiated by the addition of the adenosine deaminase inhibitor, deoxycoformycin. The results indicate that these cores act as prodrugs and that the active metabolites are their corresponding nucleosides.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Hubbell HR,Pequignot EC,Willis DH,Lee C,Suhadolnik RJsubject
Has Abstractpub_date
1985-09-15 00:00:00pages
389-94issue
3eissn
0020-7136issn
1097-0215journal_volume
36pub_type
杂志文章abstract::The variants within the human melanocortin 1 receptor (MC1R) gene are associated with an increased risk of different skin cancers. In this study, we genotyped by direct sequencing, 529 cases of basal cell carcinoma of the skin (BCC) and 533 healthy controls for polymorphisms in the entire MC1R gene. In addition to 10 ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23257
更新日期:2008-04-15 00:00:00
abstract::Screening for cancer has to be carefully organized for maximum effectiveness, and introduced in full understanding of the natural history of the disease. There are major potential harms as well as benefits from screening. The current state of art for breast, cervix and prostate cancer screening is reviewed, only for b...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.25081
更新日期:2010-03-01 00:00:00
abstract::Cyclin D1 is a major positive regulator of the G1 restriction point promoting inactivation of the retinoblastoma protein (RB). The cyclin D1 gene is rearranged, amplified and/or over-expressed in several human neoplasms. In the present series of 64 human breast carcinomas, cyclin D1 amplification (4- to 8-fold) was se...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970422)74:2<171::aid-ijc
更新日期:1997-04-22 00:00:00
abstract::A panel of monoclonal antibodies (MAbs) has been obtained, directed against determinants of normal human colorectal epithelium. BALB/c mice were immunized and boosted with mucosal scrapings and cell membrane preparations from normal large intestine. In one case boosting was also performed with HT29 colon carcinoma cel...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910390309
更新日期:1987-03-15 00:00:00
abstract::The relationship of a basement membrane collagen degrading enzyme (BM collagenase) and plasminogen activator (PA) was studied in a number of non-malignant and malignant human and murine cell lines. Several non-malignant cell lines secreted significant amounts of PA but not detectable BM collagenase activity whereas th...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910300520
更新日期:1982-11-15 00:00:00
abstract::The role of transcript variants of cyclin D1 in cancer biology is unclear. Most tumors with high levels of cyclin D1 express 2 transcripts due to alternative splicing: one full-length transcript of 4.4 kb and one short transcript of approximately 1.7 kb. The short transcript lacks part of the 3'UTR region regulating m...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21166
更新日期:2005-11-10 00:00:00
abstract::O6-alkylguanine-DNA alkyltransferase (MGMT) represents the first line of defense against the toxic, mutagenic and carcinogenic effects of O6-alkylguanine adducts in DNA. These adducts mediate the biological activity from a series of alkylating agents, such as the tobacco-specific nitrosamines, believed to contribute t...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21790
更新日期:2006-07-15 00:00:00
abstract::Anti-idiotypic antibodies that mimic tumour-associated antigens can stimulate anti-tumour T-cell responses. In this article, we have studied the role of Fc in the presentation of T-cell epitopes by 2 anti-idiotypic antibodies, 105AD7 and 708. The human monoclonal antibody 105AD7, which mimics CD55, stimulated strong i...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1194
更新日期:2001-05-01 00:00:00
abstract::Natural killer (NK) activity of human peripheral blood lymphocytes (HPBL) was studied in 32 alcoholics and 15 control subjects in a 4-h chromium release assay using K562 tumor target cells. The natural killer activity of human peripheral blood lymphocytes in alcoholics was significantly higher than the level found in ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910260405
更新日期:1980-10-15 00:00:00
abstract::Murine macrophages from different anatomical sites were compared for their ability to become tumoricidal and to secrete interleukin-1 (IL-1) and tumor necrosis factor (TNF) following stimulation in vitro by several biological response modifiers (BRM). Peritoneal macrophages (PM), alveolar macrophages (AM), and tumor-i...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910480513
更新日期:1991-07-09 00:00:00
abstract::Commercial cow milk contains considerable amounts of estrogens. Our study assessed the effect of commercial low-fat milk on the development of 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary tumors in rats. Eighty 6-week-old female Sprague-Dawley rats received a single oral dose of 5 mg DMBA. Twenty-four hours l...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20172
更新日期:2004-07-01 00:00:00
abstract::Irinotecan-based chemotherapy with bevacizumab is one of the first-line standard therapies for metastatic colorectal cancer (mCRC). TEGAFIRI (UFT/LV + irinotecan) is an irinotecan-based chemotherapy regimen. Currently, few clinical data regarding TEGAFIRI are available. This study evaluated the efficacy and safety of ...
journal_title:International journal of cancer
pub_type: 杂志文章,随机对照试验
doi:10.1002/ijc.30127
更新日期:2016-08-15 00:00:00
abstract::In normal somatic cells, the methylation pattern of DNA is stably maintained by DNA (cytosine-5-)-methyltransferase (DNA methyltransferase). Increased expression of DNA methyltransferase has been detected in many types of human cancer and has been thought to play an important role in tumorigenesis. In our study, we de...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990924)83:1<10::aid-ijc3
更新日期:1999-09-24 00:00:00
abstract::Changes in the length of a polymorphic trinucleotide (CAG) repeat in the androgen receptor (AR) gene, which may lead to altered transactivation of the AR gene, have been implicated to play a role in the pathogenesis of several forms of endocrine cancer and certain reproductive disorders. Subjects with reproductive dis...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10680
更新日期:2002-11-10 00:00:00
abstract::RB1 gene expression has been reported to be upregulated in colorectal carcinomas (CRC) at both the mRNA and protein levels when compared to normal colonic mucosa. However, allelic loss at the genomic level has been detected in CRC with widely differing frequencies ranging from 11.5% to 50%. To determine whether there ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21945
更新日期:2006-09-01 00:00:00
abstract::It has become axiomatic that critical windows of susceptibility to genotoxins exist and that genetic damage in utero may be a trigger for later life cancers. Data supporting this critical window hypothesis are remarkably few. This study provides a quantitative bridge between DNA damage by the liver carcinogen aflatoxi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29102
更新日期:2015-03-15 00:00:00
abstract::We performed an integrated array comparative genomic hybridization (aCGH) and expression microarray analysis of 8 normal gastric tissues and 38 primary tumors, including 25 intestinal and 13 diffuse gastric adenocarcinomas to identify genes whose expression is deregulated in association with copy number alteration. Ou...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23574
更新日期:2008-08-15 00:00:00
abstract::Innate lymphoid cells (ILCs) promptly initiate cytokine responses to pathogen exposure in the mucosa and mucosal-associated lymphoid tissues. ILCs were recently categorized as being of the lymphoid lineage and have been classified into three groups. ILCs play important roles in immunity against pathogens, and an anti-...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.29443
更新日期:2016-01-01 00:00:00
abstract::Proliferating cell nuclear antigen (PCNA) expression was studied by immunohistochemistry on paraffin-embedded sections of 293 primary colorectal adenocarcinomas and 56 corresponding lymph node metastases. PCNA-positive expression was detected in <25% of tumour cells in 172 (59%) cases and in > 25% in 121 (41%) cases. ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960220)69:1<5::AID-IJC2>
更新日期:1996-02-20 00:00:00
abstract::Gain of chromosome 1q is a hallmark of breast cancer, and likely reflects oncogene amplification. We previously identified mitotic kinesin KIF14 (kinesin family member 14) as an overexpressed candidate oncogene in the 1q31.3-1q32.1 minimal region of genomic gain in breast cancer cell lines. KIF14 also showed high expr...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21954
更新日期:2006-09-01 00:00:00
abstract::We previously reported that ganglioside GD1a, which is highly expressed in poorly metastatic FBJ-S1 cells, inhibits the serum-induced motility of FBJ-LL cells and that the metastatic potential of FBJ-LL cells is completely suppressed by enforced GD1a expression (Hyuga et al., Int J Cancer 1999;83:685-91). We recently ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1481
更新日期:2001-11-01 00:00:00
abstract::Inbred rats were hyper-immunised against a syngeneic transplanted mammary carcinoma, Sp4, which originally arose spontaneously in a breeding female. Spleen cells from Sp4-immune rats were fused with P3-NSI-Ag4 mouse myeloma cells, and supernatants from the hybrid progeny were screened for antibody reacting with Sp4 ce...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910260311
更新日期:1980-09-15 00:00:00
abstract::Endometrial cancer (EC) shares some environmental or genetic risk factors with colorectal cancer (CRC). It represents a risk factor for CRC. Furthermore, EC is the most frequent extracolonic neoplasm in HNPCC (hereditary nonpolyposis colorectal cancer) and, in this syndrome, it has the same inheritance pattern as CRC....
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980703)77:1<29::aid-ijc6
更新日期:1998-07-03 00:00:00
abstract::A segment of 712 bases coding for part of the human stearoyl-CoA desaturase gene was made by polymerase chain reaction (PCR) using primers based on published rat cDNA sequences. The human PCR product was confirmed by DNA sequencing. It was next cloned into a vector from which anti-sense, highly radioactive RNA transcr...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910570310
更新日期:1994-05-01 00:00:00
abstract::Leukocyte-transforming agents were isolated in baboon leukocytes inoculated with oral excretions from immunosuppressed chimpanzees. The transformed lymphoblasts had B cell surface markers and harbored herpes-type virus particles; 5-10% of the cells contained cytoplasmic antigens reactive with Epstein-Barr virus (EBV)-...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910200318
更新日期:1977-09-15 00:00:00
abstract::Regional lymph-node (LN) lymphocytes may constitute an important defence against the spread of human melanoma beyond regional LNs. The present study was directed to clonal analysis of lymphocytes cultured either directly from the LNs or after stimulation in cultures with autologous melanoma (MLTC). T-cell clones deriv...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910510209
更新日期:1992-05-08 00:00:00
abstract::We have established 3 new EBV-negative cell lines, designated Sc-1, Ri-1 and Ci-1, from patients with B-cell lymphoma/leukemia. We characterized them by cytogenetics and by study of surface membrane antigens with a panel of monoclonal antibodies (MAbs), surface and cytoplasmic immunoglobulin (Ig) expression and Ig hea...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910390116
更新日期:1987-01-15 00:00:00
abstract::In vitro, tumor-selective Hsp70 plasma membrane localization correlates with increased sensitivity to lysis mediated by a subpopulation of human natural killer (NK) cells that adhere to plastic following cytokine stimulation. In the present study, we analyzed the capacity of adoptively transferred human NK cells in SC...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/1097-0215(20001201)88:5<791::aid-ijc17>3.0
更新日期:2000-12-01 00:00:00
abstract::Initial studies have investigated the association between inflammation and colorectal cancer (CRC) using C-reactive protein (CRP) as a proinflammatory biomarker and have noted inconsistent results among women. We here report the findings from a large prospective study with repeat measurements of CRP, as well as serum ...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.27942
更新日期:2013-06-01 00:00:00
abstract::The aim of this register-based follow-up study was to evaluate the long-term cumulative incidence of second primary tumors (SPT) among survivors of hereditary retinoblastoma, with special interest for the incidence of pineoblastoma in retinoblastoma patients born after 1970. The Dutch Retinoblastoma Register was compl...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960807)67:4<515::AID-IJC
更新日期:1996-08-07 00:00:00