Abstract:
:N-0437, a non-catecholic aminotetralin has recently been described as a very potent and selective dopamine D-2 agonist. In this study the in vitro binding of [3H]N-0437 (specific activity 80.6 Ci/mmol) to calf caudate membranes is described. It was found that [3H]N-0437 binds with a high affinity (KD = 0.17 nM) and a low proportion of non-specific binding. Moreover the binding was saturable with a high number of binding sites (Bmax = 703 +/- 28 fmol/mg protein) and reversible (dissociation half-time = 68 min). Pharmacological analysis of [3H]N-0437 binding showed that it was selective for dopamine receptors and that it was also stereoselective for D-2 receptors. Non-dopaminergic drugs were without exception very poor displacers. Taken together the results suggest that [3H]N-0437 labels dopamine D-2 receptors with a high selectivity in the calf brain, and thus, that it should be a useful tool in studies of central dopamine receptors.
journal_name
Eur J Pharmacoljournal_title
European journal of pharmacologyauthors
Van der Weide J,De Vries JB,Tepper PG,Horn ASdoi
10.1016/0014-2999(87)90167-1subject
Has Abstractpub_date
1987-02-10 00:00:00pages
211-9issue
2eissn
0014-2999issn
1879-0712pii
0014-2999(87)90167-1journal_volume
134pub_type
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