Abstract：
:Assay conditions in determining total cytochrome P-450 in four laboratories were compared. Although the determination was derived from the original Omura and Sato method in each laboratory, the four standard protocols differed slightly, resulting in considerable differences in the results. Since the cytochrome P-450 content is usually expressed per mg protein, the protein assay conditions were evaluated as well. Furthermore, we compared the cytochrome P-450 values obtained by the CO- and the dithionite (DT)-difference methods. The effect of a number of variables in the assay was investigated. The influence of the storage temperature of the microsomes was ascertained as well as effects of the gassing time with CO and the time between addition of dithionite, CO-gassing and the recording of the difference spectra. After evaluating these variables a standard operation procedure was established. Using this procedure the interlaboratory coefficient of variation for total cytochrome P-450 was 4.8%, a value which was comparable to the intralaboratory coefficients of variation. The final results also show that the millimolar extinction coefficient for the DT-difference method is higher than for the CO-difference method.
journal_name
Arch Toxicoljournal_title
Archives of toxicologyauthors
Rutten AA,Falke HE,Catsburg JF,Topp R,Blaauboer BJ,van Holsteijn I,Doorn L,van Leeuwen FXdoi
10.1007/BF00324544subject
Has Abstractpub_date
1987-01-01 00:00:00pages
27-33issue
1eissn
0340-5761issn
1432-0738journal_volume
61pub_type
杂志文章abstract：:Chondrotoxicity is a rare toxicological finding which is observed in dogs after administration of quinolone antibacterials. To study this effect chondrocytes from articular cartilage of dogs were isolated, and incubated with quinolone derivatives. The effects on cell viability, mitochondrial dehydrogenase, and proteog...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF01977402
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abstract：:Age-related macular degeneration (AMD) is a late-onset retinal disease and the leading cause of central vision loss in the elderly. Degeneration of retinal pigment epithelial cells (RPE) is a crucial contributing factor responsible for the onset and progression of AMD. The toxic fluorophore N-retinyl-N-retinylidene et...
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更新日期：2020-02-01 00:00:00
abstract：:2,3,7,8-Tetrachlorodibenzo- p-dioxin (TCDD) is known to influence vitamin A homeostasis. In order to investigate the mechanism behind this retinoid disruption, male Sprague-Dawley rats were exposed to TCDD at doses ranging from 0.1 to 100 micro g/kg body weight, and were killed 3 days after exposure. Additional groups...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-003-0457-8
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abstract：:Groups of rats were exposed simultaneously to manganese chloride (3 mg Mn2+/ml water) through drinking water and lead acetate intraperitoneally at dosages of 5.0, 8.0 and 12.0 mgPb2+/kg daily for a period of 14 days. The magnitude of changes in the behavioral pattern, contents of biogenic amines and accumulation of le...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00352071
更新日期：1981-11-01 00:00:00
abstract：:The effects of cadmium exposure during pregnancy (by means of daily subcutaneous injections of 4.4 mumol/kg to the mother) on the neonates were investigated. No effect was observed on fetal or neonatal body weights, nor on neonatal liver weights. These parameters were examined up to 5 weeks after birth. The weight of ...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00334632
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pub_type： 杂志文章
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-005-0678-0
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF01973308
更新日期：1993-01-01 00:00:00
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pub_type： 杂志文章,评审
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF01974690
更新日期：1990-01-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00332484
更新日期：1988-01-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-019-02636-x
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-007-0192-7
更新日期：2007-09-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00310329
更新日期：1978-08-09 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00349074
更新日期：1984-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s002040050409
更新日期：1997-01-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF01891970
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pub_type： 杂志文章
doi：10.1007/BF01968966
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journal_title：Archives of toxicology
pub_type： 杂志文章
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更新日期：2018-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00204-005-0016-6
更新日期：2006-02-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s002040050599
更新日期：1999-04-01 00:00:00
abstract：UNLABELLED:An overdose of acetaminophen (APAP) causes hepatotoxicity due to its metabolite, N-acetyl-p-benzoquinone imine. NAD(P)H:quinone oxidoreductase 1 (NQO1) is an important enzyme for detoxification, because it catabolizes endogenous/exogenous quinone to hydroquinone. Although various studies have suggested the ...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-014-1340-5
更新日期：2015-11-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s002040050677
更新日期：1999-09-01 00:00:00
abstract：:Trichloroethylene (TCE)-induced liver toxicity and carcinogenesis is believed to be mediated in part by activation of the peroxisome proliferator-activated receptor α (PPARα). However, the contribution of the two TCE metabolites, dichloroacetate (DCA) and trichloroacetate (TCA) to the toxicity of TCE, remains unclear....
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-013-1053-1
更新日期：2013-11-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
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更新日期：1997-01-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-010-0518-8
更新日期：2010-06-01 00:00:00
abstract：: ...
journal_title：Archives of toxicology
pub_type： 评论,信件
doi：10.1007/s00204-018-2384-8
更新日期：2019-01-01 00:00:00
abstract：:In case of mycotoxin contaminations, food and feedstuff are usually contaminated by more than one toxin. However toxicological data concerning the effects of mycotoxin combinations are sparse. The intestinal epithelium is the first barrier against food contaminants and this constantly renewing organ is particularly se...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-014-1309-4
更新日期：2015-08-01 00:00:00
