High-resolution imaging reveals compartmentalization of mitochondrial protein synthesis in cultured human cells.

Abstract:

:Human mitochondria contain their own genome, mitochondrial DNA, that is expressed in the mitochondrial matrix. This genome encodes 13 vital polypeptides that are components of the multisubunit complexes that couple oxidative phosphorylation (OXPHOS). The inner mitochondrial membrane that houses these complexes comprises the inner boundary membrane that runs parallel to the outer membrane, infoldings that form the cristae membranes, and the cristae junctions that separate the two. It is in these cristae membranes that the OXPHOS complexes have been shown to reside in various species. The majority of the OXPHOS subunits are nuclear-encoded and must therefore be imported from the cytosol through the outer membrane at contact sites with the inner boundary membrane. As the mitochondrially encoded components are also integral members of these complexes, where does protein synthesis occur? As transcription, mRNA processing, maturation, and at least part of the mitoribosome assembly process occur at the nucleoid and the spatially juxtaposed mitochondrial RNA granules, is protein synthesis also performed at the RNA granules close to these entities, or does it occur distal to these sites? We have adapted a click chemistry-based method coupled with stimulated emission depletion nanoscopy to address these questions. We report that, in human cells in culture, within the limits of our methodology, the majority of mitochondrial protein synthesis is detected at the cristae membranes and is spatially separated from the sites of RNA processing and maturation.

authors

Zorkau M,Albus CA,Berlinguer-Palmini R,Chrzanowska-Lightowlers ZMA,Lightowlers RN

doi

10.1073/pnas.2008778118

subject

Has Abstract

pub_date

2021-02-09 00:00:00

issue

6

eissn

0027-8424

issn

1091-6490

pii

2008778118

journal_volume

118

pub_type

杂志文章
  • Body wall development in lamprey and a new perspective on the origin of vertebrate paired fins.

    abstract::Classical hypotheses regarding the evolutionary origin of paired appendages propose transformation of precursor structures (gill arches and lateral fin folds) into paired fins. During development, gnathostome paired appendages form as outgrowths of body wall somatopleure, a tissue composed of somatic lateral plate mes...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1304210110

    authors: Tulenko FJ,McCauley DW,Mackenzie EL,Mazan S,Kuratani S,Sugahara F,Kusakabe R,Burke AC

    更新日期:2013-07-16 00:00:00

  • A gene atlas of the mouse and human protein-encoding transcriptomes.

    abstract::The tissue-specific pattern of mRNA expression can indicate important clues about gene function. High-density oligonucleotide arrays offer the opportunity to examine patterns of gene expression on a genome scale. Toward this end, we have designed custom arrays that interrogate the expression of the vast majority of pr...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0400782101

    authors: Su AI,Wiltshire T,Batalov S,Lapp H,Ching KA,Block D,Zhang J,Soden R,Hayakawa M,Kreiman G,Cooke MP,Walker JR,Hogenesch JB

    更新日期:2004-04-20 00:00:00

  • Nuclear targeting of the growth hormone receptor results in dysregulation of cell proliferation and tumorigenesis.

    abstract::Growth hormone receptor (GHR) has been demonstrated to be nuclear localized both in vivo and in vitro, but the significance of this observation has remained elusive. Here we show that nuclear GHR is strongly correlated with proliferative status in vivo by using a liver regeneration model. In vitro, nuclear translocati...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0600181104

    authors: Conway-Campbell BL,Wooh JW,Brooks AJ,Gordon D,Brown RJ,Lichanska AM,Chin HS,Barton CL,Boyle GM,Parsons PG,Jans DA,Waters MJ

    更新日期:2007-08-14 00:00:00

  • Structure of toroidal DNA collapsed inside the phage capsid.

    abstract::The structure of DNA toroids made of individual DNA molecules of various lengths (3,000 to 55,000 bp) was studied, by using partially filled bacteriophage capsids in conjunction with cryoelectron microscopy. The tetravalent cation spermine was diffused through the capsid to condense the DNA under conditions that were ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0901240106

    authors: Leforestier A,Livolant F

    更新日期:2009-06-09 00:00:00

  • CD14-expressing cancer cells establish the inflammatory and proliferative tumor microenvironment in bladder cancer.

    abstract::Nonresolving chronic inflammation at the neoplastic site is consistently associated with promoting tumor progression and poor patient outcomes. However, many aspects behind the mechanisms that establish this tumor-promoting inflammatory microenvironment remain undefined. Using bladder cancer (BC) as a model, we found ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1424795112

    authors: Cheah MT,Chen JY,Sahoo D,Contreras-Trujillo H,Volkmer AK,Scheeren FA,Volkmer JP,Weissman IL

    更新日期:2015-04-14 00:00:00

  • IL-17 production from activated T cells is required for the spontaneous development of destructive arthritis in mice deficient in IL-1 receptor antagonist.

    abstract::IL-17 is a T cell-derived, proinflammatory cytokine that is suspected to be involved in the development of various inflammatory diseases. Although there are elevated levels of IL-17 in synovial fluid of patients with rheumatoid arthritis, the pathogenic role of IL-17 in the development of rheumatoid arthritis remains ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1035999100

    authors: Nakae S,Saijo S,Horai R,Sudo K,Mori S,Iwakura Y

    更新日期:2003-05-13 00:00:00

  • Brain renin-angiotensin system blockade by systemically active aminopeptidase A inhibitors: a potential treatment of salt-dependent hypertension.

    abstract::The hyperactivity of the brain renin-angiotensin system (RAS) has been implicated in the development and maintenance of hypertension in several types of experimental and genetic hypertension animal models. We previously reported that in the murine brain, aminopeptidase A (APA) is involved in the conversion of angioten...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0402312101

    authors: Fournie-Zaluski MC,Fassot C,Valentin B,Djordjijevic D,Reaux-Le Goazigo A,Corvol P,Roques BP,Llorens-Cortes C

    更新日期:2004-05-18 00:00:00

  • Nitrosative stress: metabolic pathway involving the flavohemoglobin.

    abstract::Nitric oxide (NO) biology has focused on the tightly regulated enzymatic mechanism that transforms L-arginine into a family of molecules, which serve both signaling and defense functions. However, very little is known of the pathways that metabolize these molecules or turn off the signals. The paradigm is well exempli...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.95.24.14100

    authors: Hausladen A,Gow AJ,Stamler JS

    更新日期:1998-11-24 00:00:00

  • E2F1 sumoylation as a protective cellular mechanism in oxidative stress response.

    abstract::Oxidative stress is a ubiquitous threat to all aerobic organisms and has been implicated in numerous pathological conditions such as cancer. Here we demonstrate a pivotal role for E2F1, a cell cycle regulatory transcription factor, in cell tolerance of oxidative stress. Cells lacking E2F1 are hypersensitive to oxidati...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1921554117

    authors: Graves JD,Lee YJ,Liu K,Li G,Lin FT,Lin WC

    更新日期:2020-06-30 00:00:00

  • Antifungal activity improved by coproduction of cyclodextrins and anabaenolysins in Cyanobacteria.

    abstract::Cyclodextrins are cyclic oligosaccharides widely used in the pharmaceutical industry to improve drug delivery and to increase the solubility of hydrophobic compounds. Anabaenolysins are lipopeptides produced by cyanobacteria with potent lytic activity in cholesterol-containing membranes. Here, we identified the 23- to...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1510432112

    authors: Shishido TK,Jokela J,Kolehmainen CT,Fewer DP,Wahlsten M,Wang H,Rouhiainen L,Rizzi E,De Bellis G,Permi P,Sivonen K

    更新日期:2015-11-03 00:00:00

  • Effects of systemic multiexon skipping with peptide-conjugated morpholinos in the heart of a dog model of Duchenne muscular dystrophy.

    abstract::Duchenne muscular dystrophy (DMD) is a lethal genetic disorder caused by an absence of the dystrophin protein in bodywide muscles, including the heart. Cardiomyopathy is a leading cause of death in DMD. Exon skipping via synthetic phosphorodiamidate morpholino oligomers (PMOs) represents one of the most promising ther...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1613203114

    authors: Echigoya Y,Nakamura A,Nagata T,Urasawa N,Lim KRQ,Trieu N,Panesar D,Kuraoka M,Moulton HM,Saito T,Aoki Y,Iversen P,Sazani P,Kole R,Maruyama R,Partridge T,Takeda S,Yokota T

    更新日期:2017-04-18 00:00:00

  • Exceptionally stable, redox-active supramolecular protein assemblies with emergent properties.

    abstract::The designed assembly of proteins into well-defined supramolecular architectures not only tests our understanding of protein-protein interactions, but it also provides an opportunity to tailor materials with new physical and chemical properties. Previously, we described that RIDC3, a designed variant of the monomeric ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1319866111

    authors: Brodin JD,Carr JR,Sontz PA,Tezcan FA

    更新日期:2014-02-25 00:00:00

  • Monolayer purification: a rapid method for isolating protein complexes for single-particle electron microscopy.

    abstract::Visualizing macromolecular complexes by single-particle electron microscopy (EM) entails stringent biochemical purification, specimen preparation, low-dose imaging, and 3D image reconstruction. Here, we introduce the "monolayer purification" method, which employs nickel-nitrilotriacetic acid (Ni-NTA) functionalized li...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0800867105

    authors: Kelly DF,Dukovski D,Walz T

    更新日期:2008-03-25 00:00:00

  • MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms.

    abstract::MicroRNAs (miRNAs) are endogenously encoded small noncoding RNAs, derived by processing of short RNA hairpins, that can inhibit the translation of mRNAs bearing partially complementary target sequences. In contrast, small interfering RNAs (siRNAs), which are derived by processing of long double-stranded RNAs and are o...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1630797100

    authors: Zeng Y,Yi R,Cullen BR

    更新日期:2003-08-19 00:00:00

  • Homeotic genes and the arthropod head: expression patterns of the labial, proboscipedia, and Deformed genes in crustaceans and insects.

    abstract::cDNA fragments of the homologues of the Drosophila head homeotic genes labial (lab), proboscipedia (pb), and Deformed (Dfd) have been isolated from the crustacean Porcellio scaber. Because the accumulation domains of the head homeotic complex (Hox) genes had not been previously reported for crustaceans, we studied the...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.96.18.10224

    authors: Abzhanov A,Kaufman TC

    更新日期:1999-08-31 00:00:00

  • Role of cyclophilin A in the uptake of HIV-1 by macrophages and T lymphocytes.

    abstract::Cyclophilins are a family of proteins that bind cyclosporin A (CsA) and possess peptidyl-prolyl cis-trans isomerase activity. In addition, they are secreted by activated cells and act in a cytokine-like manner, presumably via signaling through a cell surface cyclophilin receptor. More recently, host-derived cyclophili...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.95.4.1758

    authors: Sherry B,Zybarth G,Alfano M,Dubrovsky L,Mitchell R,Rich D,Ulrich P,Bucala R,Cerami A,Bukrinsky M

    更新日期:1998-02-17 00:00:00

  • Endogenous S-nitrosothiols protect against myocardial injury.

    abstract::Despite substantial evidence that nitric oxide (NO) and/or endogenous S-nitrosothiols (SNOs) exert protective effects in a variety of cardiovascular diseases, the molecular details are largely unknown. Here we show that following left coronary artery ligation, mice with a targeted deletion of the S-nitrosoglutathione ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0901043106

    authors: Lima B,Lam GK,Xie L,Diesen DL,Villamizar N,Nienaber J,Messina E,Bowles D,Kontos CD,Hare JM,Stamler JS,Rockman HA

    更新日期:2009-04-14 00:00:00

  • Ubiquitylation of p53 by the APC/C inhibitor Trim39.

    abstract::Tripartite motif 39 (Trim39) is a RING domain-containing E3 ubiquitin ligase able to inhibit the anaphase-promoting complex (APC/C) directly. Through analysis of Trim39 function in p53-positive and p53-negative cells, we have found, surprisingly, that p53-positive cells lacking Trim39 could not traverse the G1/S trans...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1212047110

    authors: Zhang L,Huang NJ,Chen C,Tang W,Kornbluth S

    更新日期:2012-12-18 00:00:00

  • Regulation of sensitivity to beta-adrenergic stimulation in induction of pineal N-acetyltransferase.

    abstract::Stimulation by isoproterenol causes large increases in the activity of rat pineal N-acetyltransferase (arylamine acetyltransferase or acetyl-CoA:arylamine N-acetyltransferase, EC 2.3.1.5) after a variable lag period. Lengthening periods of exposure to light cause rapid increases in the sensitivity of pineal N-acetyltr...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.72.5.1661

    authors: Romero JA,Axelrod J

    更新日期:1975-05-01 00:00:00

  • Regulation of molecular components of the synapse in the developing and adult rat superior cervical ganglion.

    abstract::Rat superior cervical sympathetic ganglion was used to begin studying the regulation of molecular components of the synapse. Ganglionic postsynaptic densities (PSDs)exhibited a thin, disc-shaped profile electron microscopically, comparable to that described for brain. Moreover, the presumptive ganglionic PSD protein (...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.84.23.8687

    authors: Wu K,Black IB

    更新日期:1987-12-01 00:00:00

  • Interleukin 12 suppresses autoantibody production by reversing helper T-cell phenotype in hepatitis B e antigen transgenic mice.

    abstract::Helper T (Th) cells are classified as Th1 or Th2 cells by virtue of cytokine secretion and function as mediators of cellular or humoral immunity, respectively. Cytokines also regulate the differentiation of Th cells. For example, interleukin (IL)-12 promotes Th1 and suppresses Th2 cell development, suggesting that IL-...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.92.15.6847

    authors: Milich DR,Wolf SF,Hughes JL,Jones JE

    更新日期:1995-07-18 00:00:00

  • Genetic lesions induced by chemicals in spermatozoa and spermatids of mice are repaired in the egg.

    abstract::Conclusive proof that the mouse egg is capable of carrying out repair of genetic lesions present in the male genome was obtained through dominant-lethal studies of chemically treated spermatozoa and spermatids and through cytological analysis of first-cleavage metaphases. The maximum difference in repair capability be...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.76.1.435

    authors: Generoso WM,Cain KT,Krishna M,Huff SW

    更新日期:1979-01-01 00:00:00

  • Regulation of p53 is critical for vertebrate limb regeneration.

    abstract::Extensive regeneration of the vertebrate body plan is found in salamander and fish species. In these organisms, regeneration takes place through reprogramming of differentiated cells, proliferation, and subsequent redifferentiation of adult tissues. Such plasticity is rarely found in adult mammalian tissues, and this ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1310519110

    authors: Yun MH,Gates PB,Brockes JP

    更新日期:2013-10-22 00:00:00

  • Predominance of hemodynamic rather than metabolic factors in the pathogenesis of diabetic glomerulopathy.

    abstract::Six groups of Munich-Wistar rats underwent micropuncture study 2-10 weeks and morphologic studies 11-13 months after induction of streptozotocin diabetes or after sham treatment. Diabetic rats received diets containing 6% (group D6), 12% (D12), or 50% protein (D50) and were maintained under similar conditions of moder...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.82.17.5963

    authors: Zatz R,Meyer TW,Rennke HG,Brenner BM

    更新日期:1985-09-01 00:00:00

  • A novel murine oncornavirus with dual eco- and xenotropic properties.

    abstract::Infection of Swiss mouse 3T3FL cells with a clonal isolate of Moloney leukemia virus (MLV-IC) resulted in virus progeny composed of at least three different murine helper oncornaviruses. Each entity was purified in appropriate cells by several sequential terminal dilution isolations and was grouwn to high titers. Besi...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.72.12.5150

    authors: Fischinger PJ,Nomura S,Bolognesi DP

    更新日期:1975-12-01 00:00:00

  • Cloning of cDNAs that encode human mast cell carboxypeptidase A, and comparison of the protein with mouse mast cell carboxypeptidase A and rat pancreatic carboxypeptidases.

    abstract::Human skin and lung mast cells and rodent peritoneal mast cells contain a carboxypeptidase in their secretory granules. We have screened human lung cDNA libraries with a mouse mast cell carboxypeptidase A (MC-CPA) cDNA probe to isolate a near-full-length cDNA that encodes human MC-CPA. The 5' end of the human MC-CPA t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.86.23.9480

    authors: Reynolds DS,Gurley DS,Stevens RL,Sugarbaker DJ,Austen KF,Serafin WE

    更新日期:1989-12-01 00:00:00

  • Knock-in human rhodopsin-GFP fusions as mouse models for human disease and targets for gene therapy.

    abstract::The human rhodopsin gene is the locus for numerous alleles linked to the neurodegenerative disease retinitis pigmentosa. To facilitate the study of retinal degeneration and to test reagents designed to alter the structure and function of this gene, we have developed strains of mice whose native rhodopsin gene has been...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0403149101

    authors: Chan F,Bradley A,Wensel TG,Wilson JH

    更新日期:2004-06-15 00:00:00

  • Crystal structure of HLA-G: a nonclassical MHC class I molecule expressed at the fetal-maternal interface.

    abstract::HLA-G is a nonclassical major histocompatibility complex class I (MHC-I) molecule that is primarily expressed at the fetal-maternal interface, where it is thought to play a role in protecting the fetus from the maternal immune response. HLA-G binds a limited repertoire of peptides and interacts with the inhibitory leu...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0409676102

    authors: Clements CS,Kjer-Nielsen L,Kostenko L,Hoare HL,Dunstone MA,Moses E,Freed K,Brooks AG,Rossjohn J,McCluskey J

    更新日期:2005-03-01 00:00:00

  • Coarse-grained analysis of stochasticity-induced switching between collective motion states.

    abstract::A single animal group can display different types of collective motion at different times. For a one-dimensional individual-based model of self-organizing group formation, we show that repeated switching between distinct ordered collective states can occur entirely because of stochastic effects. We introduce a framewo...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0608270104

    authors: Kolpas A,Moehlis J,Kevrekidis IG

    更新日期:2007-04-03 00:00:00

  • Evolution of models to support community and policy action with science: Balancing pastoral livelihoods and wildlife conservation in savannas of East Africa.

    abstract::We developed a "continual engagement" model to better integrate knowledge from policy makers, communities, and researchers with the goal of promoting more effective action to balance poverty alleviation and wildlife conservation in 4 pastoral ecosystems of East Africa. The model involved the creation of a core boundar...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0900313106

    authors: Reid RS,Nkedianye D,Said MY,Kaelo D,Neselle M,Makui O,Onetu L,Kiruswa S,Kamuaro NO,Kristjanson P,Ogutu J,BurnSilver SB,Goldman MJ,Boone RB,Galvin KA,Dickson NM,Clark WC

    更新日期:2016-04-26 00:00:00