Abstract：
:Biological computation requires in vivo control of molecular behavior to progress development of autonomous devices. miRNA switches represent excellent, easily engineerable synthetic biology tools to achieve user-defined gene regulation. Here we present the construction of a synthetic network to implement detoxification functionality. We employed a modular design strategy by engineering toxin-induced control of an enzyme scavenger. Our miRNA switch results show moderate synthetic expression control over a biologically active detoxification enzyme molecule, using an established design protocol. However, following a new design approach, we demonstrated an evolutionarily designed miRNA switch to more effectively activate enzyme activity than synthetically designed versions, allowing markedly improved extrinsic user-defined control with a toxin as inducer. Our straightforward new design approach is simple to implement and uses easily accessible web-based databases and prediction tools. The ability to exert control of toxicity demonstrates potential for modular detoxification systems that provide a pathway to new therapeutic and biocomputing applications.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Pollak NM,Cooper-White JJ,Macdonald Jdoi
10.1038/s41598-021-81679-6subject
Has Abstractpub_date
2021-01-28 00:00:00pages
2462issue
1issn
2045-2322pii
10.1038/s41598-021-81679-6journal_volume
11pub_type
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