当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES期刊下所有文献 > Aptamers against the β-Conglutin Allergen: Insights into the Behavior of the Shortest Multimeric (Intra)Molecular DNA G-Quadruplex.

Aptamers against the β-Conglutin Allergen: Insights into the Behavior of the Shortest Multimeric (Intra)Molecular DNA G-Quadruplex.

Abstract：

:In previous work, a 93-mer aptamer was selected against the anaphylactic allergen, β-conglutin and truncated to an 11-mer, improving the affinity by two orders of magnitude, whilst maintaining the specificity. This 11-mer was observed to fold in a G-quadruplex, and preliminary results indicated the existence of a combination of monomeric and higher-order structures. Building on this previous work, in the current study, we aimed to elucidate a deeper understanding of the structural forms of this 11-mer and the effect of the structure on its binding ability. A battery of techniques including polyacrylamide gel electrophoresis, high-performance liquid chromatography in combination with electrospray ionization time-of-flight mass spectrometry, matrix-assisted laser desorption/ionization time-of-flight, thermal binding analysis, circular dichroism and nuclear magnetic resonance were used to probe the structure of both the 11-mer and the 11-mer flanked with TT- at either the 5' or 3' end or at both ends. The TT-tail at the 5' end hinders stacking effects and effectively enforces the 11-mer to maintain a monomeric form. The 11-mer and the TT- derivatives of the 11-mer were also evaluated for their ability to bind its cognate target using microscale thermophoresis and surface plasmon resonance, and biolayer interferometry confirmed the nanomolar affinity of the 11-mer. All the techniques utilized confirmed that the 11-mer was found to exist in a combination of monomeric and higher-order structures, and that independent of the structural form present, nanomolar affinity was observed.

journal_name

Int J Mol Sci

journal_title

International journal of molecular sciences

authors

Sullivan CKO,Mairal T,Jauset-Rubio M,Svobodova M,Skouridou V,Esposito V,Virgilio A,Galeone A

doi

10.3390/ijms22031150

subject

Has Abstract

pub_date

2021-01-24 00:00:00

issue

3

issn

1422-0067

pii

ijms22031150

journal_volume

22

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES文献大全
  • Targeting NRF2 for the Treatment of Friedreich's Ataxia: A Comparison among Drugs.

    abstract：:NRF2 (Nuclear factor Erythroid 2-related Factor 2) signaling is impaired in Friedreich's Ataxia (FRDA), an autosomal recessive disease characterized by progressive nervous system damage and degeneration of nerve fibers in the spinal cord and peripheral nerves. The loss of frataxin in patients results in iron sulfur cl...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20205211

    authors： Petrillo S,D'Amico J,La Rosa P,Bertini ES,Piemonte F

    更新日期：2019-10-21 00:00:00

  • Beyond Kinase Activity: ERK5 Nucleo-Cytoplasmic Shuttling as a Novel Target for Anticancer Therapy.

    abstract：:The importance of mitogen-activated protein kinases (MAPK) in human pathology is underlined by the relevance of abnormalities of MAPK-related signaling pathways to a number of different diseases, including inflammatory disorders and cancer. One of the key events in MAPK signaling, especially with respect to pro-prolif...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms21030938

    authors： Tubita A,Lombardi Z,Tusa I,Dello Sbarba P,Rovida E

    更新日期：2020-01-31 00:00:00

  • Overcoming the recalcitrance for the conversion of kenaf pulp to glucose via microwave-assisted pre-treatment processes.

    abstract：:This study evaluates the pre-treatment of cellulose from kenaf plant to yield sugar precursors for the production of ethanol or butanol for use as biofuel additives. In order to convert the crystalline cellulosic form to the amorphous form that can undergo enzymatic hydrolysis of the glycosidic bond to yield sugars, k...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms12031451

    authors： Ooi BG,Rambo AL,Hurtado MA

    更新日期：2011-01-01 00:00:00

  • Immunogenomic Classification of Colorectal Cancer and Therapeutic Implications.

    abstract：:The immune system has a substantial effect on colorectal cancer (CRC) progression. Additionally, the response to immunotherapeutics and conventional treatment options (e.g., chemotherapy, radiotherapy and targeted therapies) is influenced by the immune system. The molecular characterization of colorectal cancer (CRC) ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms18102229

    authors： Roelands J,Kuppen PJK,Vermeulen L,Maccalli C,Decock J,Wang E,Marincola FM,Bedognetti D,Hendrickx W

    更新日期：2017-10-24 00:00:00

  • Disulfide Bond Engineering of an Endoglucanase from Penicillium verruculosum to Improve Its Thermostability.

    abstract：:Endoglucanases (EGLs) are important components of multienzyme cocktails used in the production of a wide variety of fine and bulk chemicals from lignocellulosic feedstocks. However, a low thermostability and the loss of catalytic performance of EGLs at industrially required temperatures limit their commercial applicat...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20071602

    authors： Bashirova A,Pramanik S,Volkov P,Rozhkova A,Nemashkalov V,Zorov I,Gusakov A,Sinitsyn A,Schwaneberg U,Davari MD

    更新日期：2019-03-30 00:00:00

  • Radioprotective Effects of Dermatan Sulfate in a Preclinical Model of Oral Mucositis-Targeting Inflammation, Hypoxia and Junction Proteins without Stimulating Proliferation.

    abstract：:Oral mucositis is the most frequently occurring early side effect of head-and-neck cancer radiotherapy. Systemic dermatan sulfate (DS) treatment revealed a significant radioprotective potential in a preclinical model of oral mucositis. This study was initiated to elucidate the mechanistic effects of DS in the same mod...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms19061684

    authors： Gruber S,Arnold M,Cini N,Gernedl V,Hetzendorfer S,Kowald LM,Kuess P,Mayer J,Morava S,Pfaffinger S,Rohorzka A,Dörr W

    更新日期：2018-06-06 00:00:00

  • Novel Psychoactive Phenethylamines: Impact on Genetic Material.

    abstract：:Psychedelic and stimulating phenethylamines belong to the family of new psychoactive substances (NPS). The acute toxicity framework has begun to be investigated, while studies showing genotoxic potential are very limited or not available. Therefore, in order to fill this gap, the aim of the present work was to evaluat...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21249616

    authors： Cocchi V,Gasperini S,Hrelia P,Tirri M,Marti M,Lenzi M

    更新日期：2020-12-17 00:00:00

  • Ovarian Cancer Immunotherapy: Turning up the Heat.

    abstract：:Epithelial ovarian cancer (EOC) is the leading cause of death among gynecological malignancies. Despite surgery and chemotherapy, 5-years survival rates have improved only modestly over the past few decades remaining at 45% for advanced stages. Therefore, novel therapies are urgently needed. The presence of tumor-infi...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms20122927

    authors： Ghisoni E,Imbimbo M,Zimmermann S,Valabrega G

    更新日期：2019-06-15 00:00:00

  • FRCaMP, a Red Fluorescent Genetically Encoded Calcium Indicator Based on Calmodulin from Schizosaccharomyces Pombe Fungus.

    abstract：:Red fluorescent genetically encoded calcium indicators (GECIs) have expanded the available pallet of colors used for the visualization of neuronal calcium activity in vivo. However, their calcium-binding domain is restricted by calmodulin from metazoans. In this study, we developed red GECI, called FRCaMP, using calmo...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms22010111

    authors： Subach OM,Barykina NV,Chefanova ES,Vlaskina AV,Sotskov VP,Ivashkina OI,Anokhin KV,Subach FV

    更新日期：2020-12-24 00:00:00

  • Emerging Role of Eukaryote Ribosomes in Translational Control.

    abstract：:Translation is one of the final steps that regulate gene expression. The ribosome is the effector of translation through to its role in mRNA decoding and protein synthesis. Many mechanisms have been extensively described accounting for translational regulation. However it emerged only recently that ribosomes themselve...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms20051226

    authors： Dalla Venezia N,Vincent A,Marcel V,Catez F,Diaz JJ

    更新日期：2019-03-11 00:00:00

  • A Working Hypothesis Regarding Identical Pathomechanisms between Clinical Efficacy and Adverse Reaction of Clozapine via the Activation of Connexin43.

    abstract：:Clozapine (CLZ) is an approved antipsychotic agent for the medication of treatment-resistant schizophrenia but is also well known as one of the most toxic antipsychotics. Recently, the World Health Organization's (WHO) global database (VigiBase) reported the relative lethality of severe adverse reactions of CLZ. Agran...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms21197019

    authors： Okada M,Fukuyama K,Shiroyama T,Murata M

    更新日期：2020-09-24 00:00:00

  • ceRNA Cross-Talk in Paulownia Witches' Broom Disease.

    abstract：:Long noncoding RNA (lncRNA), circular RNA (circRNA), and microRNA (miRNA) are important in the regulation of life activities. However, their function is unclear in Paulownia fortunei. To identify lncRNAs, circRNAs, and miRNA, and investigate their roles in the infection progress of Paulownia witches' broom (PaWB) dise...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms19082463

    authors： Fan G,Wang Z,Zhai X,Cao Y

    更新日期：2018-08-20 00:00:00

  • Endothelial Semaphorin 7A promotes inflammation in seawater aspiration-induced acute lung injury.

    abstract：:Inflammation is involved in the pathogenesis of seawater aspiration-induced acute lung injury (ALI). Although several studies have shown that Semaphorin 7A (SEMA7A) promotes inflammation, there are limited reports regarding immunological function of SEMA7A in seawater aspiration-induced ALI. Therefore, we investigated...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms151119650

    authors： Zhang M,Wang L,Dong M,Li Z,Jin F

    更新日期：2014-10-28 00:00:00

  • Finely-Tuned Calcium Oscillations in Osteoclast Differentiation and Bone Resorption.

    abstract：:Calcium (Ca2+) plays an important role in regulating the differentiation and function of osteoclasts. Calcium oscillations (Ca oscillations) are well-known phenomena in receptor activator of nuclear factor kappa B ligand (RANKL)-induced osteoclastogenesis and bone resorption via calcineurin. Many modifiers are involve...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms22010180

    authors： Okada H,Okabe K,Tanaka S

    更新日期：2020-12-26 00:00:00

  • Intracerebroventricular Treatment with 2-Hydroxypropyl-β-Cyclodextrin Decreased Cerebellar and Hepatic Glycoprotein Nonmetastatic Melanoma Protein B (GPNMB) Expression in Niemann-Pick Disease Type C Model Mice.

    abstract：:Niemann-Pick disease type C (NPC) is a recessive hereditary disease caused by mutation of the NPC1 or NPC2 gene. It is characterized by abnormality of cellular cholesterol trafficking with severe neuronal and hepatic injury. In this study, we investigated the potential of glycoprotein nonmetastatic melanoma protein B ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms22010452

    authors： Fukaura M,Ishitsuka Y,Shirakawa S,Ushihama N,Yamada Y,Kondo Y,Takeo T,Nakagata N,Motoyama K,Higashi T,Arima H,Kurauchi Y,Seki T,Katsuki H,Higaki K,Matsuo M,Irie T

    更新日期：2021-01-05 00:00:00

  • Overexpression of ABCB1 Transporter Confers Resistance to mTOR Inhibitor WYE-354 in Cancer Cells.

    abstract：:The overexpressing ABCB1 transporter is one of the key factors leading to multidrug resistance (MDR). Thus, many ABCB1 inhibitors have been found to be able to overcome ABCB1-mediated MDR. However, some inhibitors also work as a substrate of ABCB1, which indicates that in order to achieve an effective reversal dosage,...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21041387

    authors： Wang J,Yang DH,Yang Y,Wang JQ,Cai CY,Lei ZN,Teng QX,Wu ZX,Zhao L,Chen ZS

    更新日期：2020-02-19 00:00:00

  • Low-Grade Inflammation Is Associated with Apathy Indirectly via Deep White Matter Lesions in Community-Dwelling Older Adults: The Sefuri Study.

    abstract：:Low-grade inflammation is implicated in the pathogenesis of atherosclerosis, metabolic syndrome, and apathy as a form of vascular depression. We analyzed the brain magnetic resonance imaging findings in 259 community-dwelling older adults (122 men and 137 women, with a mean age of 68.4 years). The serum concentrations...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20081905

    authors： Yao H,Mizoguchi Y,Monji A,Yakushiji Y,Takashima Y,Uchino A,Yuzuriha T,Hashimoto M

    更新日期：2019-04-17 00:00:00

  • Bistable switch in let-7 miRNA biogenesis pathway involving Lin28.

    abstract：:miRNAs are small noncoding RNAs capable of regulating gene expression at the post-transcriptional level. A growing body of evidence demonstrated that let-7 family of miRNAs, as one of the highly conserved miRNAs, plays an important role in cell differentiation and development, as well as tumor suppressor function depe...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms151019119

    authors： Shi F,Yu W,Wang X

    更新日期：2014-10-21 00:00:00

  • The Role of MicroRNAs as Predictors of Response to Tamoxifen Treatment in Breast Cancer Patients.

    abstract：:Endocrine therapy is a key treatment strategy to control or eradicate hormone-responsive breast cancer. However, resistance to endocrine therapy leads to breast cancer relapse. The recent extension of adjuvant tamoxifen treatment up to 10 years actualizes the need for identifying biological markers that may be used to...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms161024243

    authors： Egeland NG,Lunde S,Jonsdottir K,Lende TH,Cronin-Fenton D,Gilje B,Janssen EA,Søiland H

    更新日期：2015-10-14 00:00:00

  • The Na/K-ATPase Signaling: From Specific Ligands to General Reactive Oxygen Species.

    abstract：:The signaling function of the Na/K-ATPase has been established for 20 years and is widely accepted in the field, with many excellent reports and reviews not cited here. Even though there is debate about the underlying mechanism, the signaling function is unquestioned. This short review looks back at the evolution of N...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms19092600

    authors： Pratt RD,Brickman CR,Cottrill CL,Shapiro JI,Liu J

    更新日期：2018-09-01 00:00:00

  • Copper Coordination Compounds as Biologically Active Agents.

    abstract：:Copper-containing coordination compounds attract wide attention due to the redox activity and biogenicity of copper ions, providing multiple pathways of biological activity. The pharmacological properties of metal complexes can be fine-tuned by varying the nature of the ligand and donor atoms. Copper-containing coordi...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms21113965

    authors： Krasnovskaya O,Naumov A,Guk D,Gorelkin P,Erofeev A,Beloglazkina E,Majouga A

    更新日期：2020-05-31 00:00:00

  • Structure prediction, molecular dynamics simulation and docking studies of D-specific dehalogenase from Rhizobium sp. RC1.

    abstract：:Currently, there is no three-dimensional structure of D-specific dehalogenase (DehD) in the protein database. We modeled DehD using ab initio technique, performed molecular dynamics (MD) simulation and docking of D-2-chloropropionate (D-2CP), D-2-bromopropionate (D-2BP), monochloroacetate (MCA), monobromoacetate (MBA)...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms131215724

    authors： Sudi IY,Wong EL,Joyce-Tan KH,Shamsir MS,Jamaluddin H,Huyop F

    更新日期：2012-11-26 00:00:00

  • Manganese(II) Oxidizing Bacteria as Whole-Cell Catalyst for β-Keto Ester Oxidation.

    abstract：:Manganese oxidizing bacteria can produce biogenic manganese oxides (BMO) on their cell surface and have been applied in the fields of agriculture, bioremediation, and drinking water treatment to remove toxic contaminants based on their remarkable chemical reactivity. Herein, we report for the first time the synthetic ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21051709

    authors： Guo J,Guo H,Liu J,Zhong F,Wu Y

    更新日期：2020-03-02 00:00:00

  • Cardiac Function is not Susceptible to Moderate Disassembly of Mitochondrial Respiratory Supercomplexes.

    abstract：:: Mitochondrial respiratory chain supercomplexes (RCS), particularly, the respirasome, which contains complexes I, III, and IV, have been suggested to participate in facilitating electron transport, reducing the production of reactive oxygen species (ROS), and maintaining the structural integrity of individual electro...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21051555

    authors： Chapa-Dubocq XR,Rodríguez-Graciani KM,Guzmán-Hernández RA,Jang S,Brookes PS,Javadov S

    更新日期：2020-02-25 00:00:00

  • Whole Exome Sequencing in Psoriasis Patients Contributes to Studies of Acitretin Treatment Difference.

    abstract：:Psoriasis vulgaris is an immune-mediated inflammatory skin disease. Although acitretin is a widely used synthetic retinoid for moderate to severe psoriasis, little is known about patients' genetics in response to this drug. In this study, 179 patients were enrolled in either the discovery set (13 patients) or replicat...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms18020295

    authors： Zhou X,He Y,Kuang Y,Li J,Zhang J,Chen M,Chen W,Su J,Zhao S,Liu P,Chen M,Shen M,Chen X,Zhu W,Chen X

    更新日期：2017-01-29 00:00:00

  • Polygonatum odoratum Polysaccharides Modulate Gut Microbiota and Mitigate Experimentally Induced Obesity in Rats.

    abstract：:Increasing evidence suggests that the gut microbiota plays vital roles in metabolic diseases. Polygonatum odoratum extract alleviates hyperglycemia and hyperlipidemia, but the underlying mechanism remains unclear. This study investigated the effects of P. odoratum polysaccharides (POPs) on high-fat diet (HFD)-induced ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms19113587

    authors： Wang Y,Fei Y,Liu L,Xiao Y,Pang Y,Kang J,Wang Z

    更新日期：2018-11-13 00:00:00

  • The Mechanism of Melatonin and Its Receptor MT2 Involved in the Development of Bovine Granulosa Cells.

    abstract：:Ovarian granulosa cells (GCs) are a critical approach to investigate the mechanism of gene regulation during folliculogenesis. The objective of this study was to investigate the role of MT2 in bovine GCs, and assess whether MT2 silencing affected GCs response to melatonin. We found that MT2 silencing significantly dec...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms19072028

    authors： Wang S,Liu W,Pang X,Dai S,Liu G

    更新日期：2018-07-12 00:00:00

  • Phenotypic Screen with the Human Secretome Identifies FGF16 as Inducing Proliferation of iPSC-Derived Cardiac Progenitor Cells.

    abstract：:Paracrine factors can induce cardiac regeneration and repair post myocardial infarction by stimulating proliferation of cardiac cells and inducing the anti-fibrotic, antiapoptotic, and immunomodulatory effects of angiogenesis. Here, we screened a human secretome library, consisting of 923 growth factors, cytokines, an...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20236037

    authors： Jennbacken K,Wågberg F,Karlsson U,Eriksson J,Magnusson L,Chimienti M,Ricchiuto P,Bernström J,Ding M,Ross-Thriepland D,Xue Y,Peiris D,Aastrup T,Tegel H,Hober S,Sivertsson Å,Uhlén M,Strömstedt PE,Davies R,Holmberg Sch

    更新日期：2019-11-30 00:00:00

  • Plant MicroRNAs in Cross-Kingdom Regulation of Gene Expression.

    abstract：:MicroRNAs (miRNAs) are a class of noncoding small RNAs, which play a crucial role in post-transcriptional gene regulation. Recently, various reports revealed that miRNAs could be transmitted between species to mediate cross-kingdom regulation by integrating into a specific target gene-mediated regulatory pathway to ex...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms19072007

    authors： Wang W,Liu D,Zhang X,Chen D,Cheng Y,Shen F

    更新日期：2018-07-10 00:00:00

  • Irradiation-Induced Upregulation of miR-711 Inhibits DNA Repair and Promotes Neurodegeneration Pathways.

    abstract：:Radiotherapy for brain tumors induces neuronal DNA damage and may lead to neurodegeneration and cognitive deficits. We investigated the mechanisms of radiation-induced neuronal cell death and the role of miR-711 in the regulation of these pathways. We used in vitro and in vivo models of radiation-induced neuronal cell...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21155239

    authors： Sabirzhanov B,Makarevich O,Barrett JP,Jackson IL,Glaser EP,Faden AI,Stoica BA

    更新日期：2020-07-23 00:00:00