Electrophilic oxysterols: generation, measurement and protein modification.

Abstract:

:Cholesterol is an essential component of mammalian plasma membrane. Alterations in sterol metabolism or oxidation have been linked to various pathological conditions, including cardiovascular diseases, cancer, and neurodegenerative disorders. Unsaturated sterols are vulnerable to oxidation induced by singlet oxygen and other reactive oxygen species. This process yields reactive sterol oxidation products, including hydroperoxides, epoxides as well as aldehydes. These oxysterols, in particular those with high electrophilicity, can modify nucleophilic sites in biomolecules and affect many cellular functions. Here, we review the generation and measurement of reactive sterol oxidation products with emphasis on cholesterol hydroperoxides and aldehyde derivatives (electrophilic oxysterols) and their effects on protein modifications.

journal_name

Free Radic Res

journal_title

Free radical research

authors

Miyamoto S,Lima RS,Inague A,Viviani LG

doi

10.1080/10715762.2021.1879387

subject

Has Abstract

pub_date

2021-01-26 00:00:00

pages

1-56

eissn

1071-5762

issn

1029-2470

pub_type

杂志文章
  • Hormones and oxidative stress: an overview.

    abstract::In aerobes, oxygen is essential for maintenance of life. However, incomplete reduction of oxygen leads to generation of reactive oxygen species. These oxidants oxidise biological macromolecules present in their vicinity and thereby impair cellular functions causing oxidative stress (OS). Aerobes have evolved both enzy...

    journal_title:Free radical research

    pub_type: 杂志文章,评审

    doi:10.1080/10715762.2019.1702656

    authors: Chainy GBN,Sahoo DK

    更新日期:2020-01-01 00:00:00

  • Nitric oxide scavenging by cell-free hemoglobin may be a primary factor determining hypertension in polycythemic patients.

    abstract::We tested the hypothesis that hypertension associated with polycythemia vera (PV) may be related to hemoglobin released from erythrocytes (cell-free hemoglobin, fHb). We assessed hematocrit, mean arterial pressure (MAP), blood viscosity, and the level of fHb and nitrite/nitrate (NOx) in the plasma of 73 PV patients an...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715762.2013.860225

    authors: Rusak T,Misztal T,Piszcz J,Tomasiak M

    更新日期:2014-02-01 00:00:00

  • Initiation of a superoxide-dependent chain oxidation of lactate dehydrogenase-bound NADH by oxidants of low and high reactivity.

    abstract::In cells, NADH and NADPH are mainly bound to dehydrogenases such as lactate dehydrogenase (LDH). In cell-free systems, the binary LDH-NADH complex has been demonstrated to produce reactive oxygen species via a chain oxidation of NADH initiated and propagated by superoxide. We studied here whether this chain radical re...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760500231786

    authors: Petrat F,Bramey T,Kirsch M,De Groot H

    更新日期:2005-10-01 00:00:00

  • Lipofuscin-mediated photic stress inhibits phagocytic activity of ARPE-19 cells; effect of donors' age and antioxidants.

    abstract::The risk of chronic oxidative stress in the retinal pigment epithelium (RPE) increases with age due to accumulation of the photoreactive age pigment lipofuscin (LFG). Here, we asked whether sublethal and weakly lethal photic stress, induced by irradiation of ARPE-19 cells containing phagocytised LFG, affected the cell...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715762.2017.1380307

    authors: Olchawa MM,Furso JA,Szewczyk GM,Sarna TJ

    更新日期:2017-10-01 00:00:00

  • EPR studies on the effects of complexation of heme by hemopexin upon its reactions with organic peroxides.

    abstract::Hemopexin, a heme-binding serum glycoprotein, is thought to play an important role in the prevention of oxidative damage that may be catalysed by free heme. Through the use of EPR techniques, the generation of free radicals from organic hydroperoxides by heme and heme-hemopexin complexes, and the concomitant formation...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715769509065277

    authors: Timmins GS,Davies MJ,Song DX,Muller-Eberhard U

    更新日期:1995-12-01 00:00:00

  • Comparison of the phenolic composition of fruit juices by single step gradient HPLC analysis of multiple components versus multiple chromatographic runs optimised for individual families.

    abstract::After minimal sample preparation, two different HPLC methodologies, one based on a single gradient reversed-phase HPLC step, the other on multiple HPLC runs each optimised for specific components, were used to investigate the composition of flavonoids and phenolic acids in apple and tomato juices. The principal compon...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760000300551

    authors: Bremner PD,Blacklock CJ,Paganga G,Mullen W,Rice-Evans CA,Crozier A

    更新日期:2000-06-01 00:00:00

  • Singlet oxygen quenching and radical scavenging capacities of structurally-related flavonoids present in Zuccagnia punctata Cav.

    abstract::The singlet oxygen (1O2) quenching and free radical (DPPH(*), ABTS(* +) and O2(* -)) scavenging ability of three structurally-related flavonoids (7-hydroxyflavanone HF, 2',4'-dihydroxychalcone DHC and 3,7-dihydroxyflavone DHF) present in the Argentinean native shrub Zuccagnia punctata Cav. were studied in solution by ...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760902912264

    authors: Vieyra FE,Boggetti HJ,Zampini IC,Ordoñez RM,Isla MI,Alvarez RM,De Rosso V,Mercadante AZ,Borsarelli CD

    更新日期:2009-06-01 00:00:00

  • Influence of an orally effective SOD on hyperbaric oxygen-related cell damage.

    abstract::In a prospective, double-blind, randomised placebo-controlled study, we tested the hypothesis that a new formulation consisting of wheat gliadin chemically combined with a vegetal (thus orally effective) preparation of superoxide dismutase (SOD) allows to prevent hyperbaric oxygen (HBO)-induced oxidative cell stress. ...

    journal_title:Free radical research

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1080/10715760412331273197

    authors: Muth CM,Glenz Y,Klaus M,Radermacher P,Speit G,Leverve X

    更新日期:2004-09-01 00:00:00

  • Cigarette smokers have decreased lymphocyte and platelet alpha-tocopherol levels and increased excretion of the gamma-tocopherol metabolite gamma-carboxyethyl-hydroxychroman (gamma-CEHC).

    abstract::Cigarette smoking is associated with increased oxidative stress and increased risk of degenerative disease. As the major lipophilic antioxidant, requirements for vitamin E may be higher in smokers due to increased utilisation. In this observational study we have compared vitamin E status in smokers and non-smokers usi...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760410001715149

    authors: Jeanes YM,Hall WL,Proteggente AR,Lodge JK

    更新日期:2004-08-01 00:00:00

  • Role of thiocyanate, bromide and hypobromous acid in hydrogen peroxide-induced apoptosis.

    abstract::We have previously reported that H2O2-induced apoptosis in HL-60 human leukemia cells takes place in the presence of chloride, requires myeloperoxidase (MPO), and occurs through oxidative reactions involving hypochlorous acid and chloramines. We now report that when chloride is replaced by the pseudohalide thiocyanate...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760310001643302

    authors: Wagner BA,Reszka KJ,McCormick ML,Britigan BE,Evig CB,Burns CP

    更新日期:2004-02-01 00:00:00

  • Overoxidation of peroxiredoxins as an immediate and sensitive marker of oxidative stress in HepG2 cells and its application to the redox effects induced by ischemia/reperfusion in human liver.

    abstract::Oxidative stress is a major pathogenetic event occurring in several liver disorders and is a major cause of liver damage due to Ischemia/Reperfusion (I/R) during liver transplantation. While several markers of chronic oxidative stress are well known, early protein targets of oxidative injury are not well defined. In o...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760400029603

    authors: Cesaratto L,Vascotto C,D'Ambrosio C,Scaloni A,Baccarani U,Paron I,Damante G,Calligaris S,Quadrifoglio F,Tiribelli C,Tell G

    更新日期:2005-03-01 00:00:00

  • SOD2 gene polymorphism and response of oxidative stress parameters in young wrestlers to a three-month training.

    abstract::The aim of the study was to analyse the effect of Val 16Ala polymorphism in SOD2 gene on oxidative stress parameters and lipid profile of the blood during a three-month wrestling training. The study included 53 Polish young wrestlers. Blood samples were collected at the beginning of the programme and following three m...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715762.2017.1327716

    authors: Jówko E,Gierczuk D,Cieśliński I,Kotowska J

    更新日期:2017-05-01 00:00:00

  • Peroxyl radical scavenging activities of hamamelitannin in chemical and biological systems.

    abstract::The antioxidative activities of hamamelitannin (2',5-di-O-galloyl-hamamelose), gallic acid and dl-alpha-tocopherol against lipid peroxyl radicals were evaluated in chemical and biological systems. The peroxyl radical scavenging activity was evaluated by electron spin resonance (ESR) method in which both spin-trapping ...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715769509147550

    authors: Masaki H,Atsumi T,Sakurai H

    更新日期:1995-05-01 00:00:00

  • Inactivation of heart dihydrolipoamide dehydrogenase by copper Fenton systems. Effect of thiol compounds and metal chelators.

    abstract::Copper Fenton systems (Cu(II)/H2O2 and Cu(II)/Asc) inactivated the lipoamide reductase and enhanced the diaphorase activity of pig-heart lipoamide dehydrogenase (LADH). Cupric ions alone were less effective. As a result of Cu(II)/H2O2 treatment, the number of titrated thiols in LADH decreased from 6 to 1 per subunit. ...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715769509147543

    authors: Gutierrez-Correa J,Stoppani AO

    更新日期:1995-03-01 00:00:00

  • Myocardial antioxidant defense mechanisms: time related changes after reperfusion of the ischemic rat heart.

    abstract::It is well known that reperfusion damage of ischemic myocardium may be attributed to alterations in the antioxidant defense system against free radical aggression. In addition, the degree of myocardial damage may depend on the duration and severity of ischemia that precedes reperfusion. We carried out serial ischemic ...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715769409147514

    authors: Porreca E,Del Boccio G,Lapenna D,Di Febbo C,Pennelli A,Cipollone F,Di Ilio C,Cuccurullo F

    更新日期:1994-03-01 00:00:00

  • Carotenoids induce apoptosis in the T-lymphoblast cell line Jurkat E6.1.

    abstract::Epidemiologically, a high-carotenoid intake via a fruit- and vegetable-rich diet is associated with a decreased risk of various forms of cancer. The mechanisms by which carotenoids exert this protective effect are controversial. In this study, we examined the potency of a range of carotenoids commonly found in human p...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760290032539

    authors: Müller K,Carpenter KL,Challis IR,Skepper JN,Arends MJ

    更新日期:2002-07-01 00:00:00

  • Red-wine beneficial long-term effect on lipids but not on antioxidant characteristics in plasma in a study comparing three types of wine--description of two O-methylated derivatives of gallic acid in humans.

    abstract::The purpose of this double clinical study was (1) to evaluate the effect of one single intake (300 ml) of red wine (RW) on the plasma antioxidant capacity (pAOC) and plasma phenolics over the 24-h time period following the intake, and (2) to compare the long-term effects of daily intakes (250 ml/d) of RW, white wine (...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760310001598097

    authors: Cartron E,Fouret G,Carbonneau MA,Lauret C,Michel F,Monnier L,Descomps B,Léger CL

    更新日期:2003-09-01 00:00:00

  • Oxidative inactivation of the proteasome in Alzheimer's disease.

    abstract::In the present study we isolated proteasome complexes from control, mild cognitive impairment (MCI), and Alzheimer's disease (AD) subjects. No significant difference in the amount of proteasomes was detected across the different groups, although impairments in chymotrypsin-like proteasome activity was observed in AD s...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760701286159

    authors: Cecarini V,Ding Q,Keller JN

    更新日期:2007-06-01 00:00:00

  • p38 MAPK and Ca2+ contribute to hydrogen peroxide-induced increase of permeability in vascular endothelial cells but ERK does not.

    abstract::To examine the involvement of p38 mitogen-activated protein kinase (p38 MAPK) and extracellular signal-regulated kinase (ERK) in the oxidative stress-induced increase of permeability in endothelial cells, the effects of a p38 MAPK inhibitor (SB203580) and ERK inhibitor (PD90859) on the H2O2-induced increase of permeab...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760100301531

    authors: Niwa K,Inanami O,Ohta T,Ito S,Karino T,Kuwabara M

    更新日期:2001-11-01 00:00:00

  • Astragaloside IV synergizes with ferulic acid to suppress hepatic stellate cells activation in vitro.

    abstract::Because hepatic fibrosis usually involves more than one pathological process, combination therapy with modalities that target aberrant signaling cascade in activated hepatic stellate cells (HSCs) represents an alternative strategy. This study evaluates the hypothesis that astragaloside IV (AS-IV) and ferulic acid (FA)...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715762.2017.1290233

    authors: Dong H,Guo H,Liang Y,Wang X,Niu Y

    更新日期:2017-02-01 00:00:00

  • Pyruvate secreted by human lymphoid cell lines protects cells from hydrogen peroxide mediated cell death.

    abstract::Reactive oxygen species (ROS) released from polymorphonuclear leukocytes and macrophages could cause DNA damage, but also induce cell death. Therefore inhibition of cell death must be an important issue for accumulation of genetic changes in lymphoid cells in inflammatory foci. Scavengers in the post culture medium of...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760000300601

    authors: Miwa H,Fujii J,Kanno H,Taniguchi N,Aozasa K

    更新日期:2000-07-01 00:00:00

  • Visualising reactive oxygen species in live mammals and revealing of ROS-related system.

    abstract::Of all the aerobic respiration by-products, cytotoxic superoxide derived from mitochondrial-leaked electrons, is the only one known to be disposed of intracellularly. Is this fate the only destiny for mitochondrial-leaked electrons? When Cynomolgus monkeys were injected intravenously with reactive oxygen species (ROS)...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715762.2019.1677902

    authors: Guo J,Xu H,Liu S,Wang Z,Dai Y,Lu J,Zheng S,Xu D,Zhou J,Ke L,Cheng X,Xu M,Zhang X,Guo Y,Lin Y,Ding W,Gao G,Wang H,Chen Q,Yu X,Chen H,Qin L,Sun X,Li Z,Zheng S,Wang J,Cheng Y,Qiu S,Hu Y,Huang P,Li

    更新日期:2019-12-01 00:00:00

  • Involvement of protein kinase Cdelta in the activation of NADPH oxidase and the phagocytosis of neutrophils.

    abstract::This experiment was performed to clarify the role of protein kinase C (PKC) delta in NADPH oxidase-dependent O(2-) production and actin polymerization followed by phagocytosis in neutrophils. Bovine neutrophils and human neutrophil-like differentiated HL-60 (dHL-60) cells were stimulated with serum-opsonized zymosan (...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760500539121

    authors: Waki K,Inanami O,Yamamori T,Nagahata H,Kuwabara M

    更新日期:2006-04-01 00:00:00

  • Alteration of antioxidants during the progression of heart disease in streptozotocin-induced diabetic rats.

    abstract::Involvement of oxidative stress is implicated in the progression of complication of diabetes mellitus. With respect to heart diseases, we have studied role of oxidative stress/antioxidants using rats treated with streptozotocin to induce diabetes (DM). Hemodynamic and echocardiographic measurements showed thickening o...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760100300231

    authors: Doi K,Sawada F,Toda G,Yamachika S,Seto S,Urata Y,Ihara Y,Sakata N,Taniguchi N,Kondo T,Yano K

    更新日期:2001-03-01 00:00:00

  • The potential of lipid-polymer nanoparticles as epigenetic and ROS control approaches for COPD.

    abstract::Chronic obstructive pulmonary disease (COPD) is a lung disease caused by an inflammatory response to various inhaled toxins, especially cigarette smoke. Reactive oxygen species (ROS) and epigenetic abnormality are intimately related to the pathology of COPD, and the overproduction of ROS results in a decrease of histo...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715762.2019.1696965

    authors: Chikuma K,Arima K,Asaba Y,Kubota R,Asayama S,Sato K,Kawakami H

    更新日期:2019-12-06 00:00:00

  • PON1 paraoxonase activity is reduced during HDL oxidation and is an indicator of HDL antioxidant capacity.

    abstract::The aim of this study was to investigate the effect of HDL oxidation on PON1 paraoxonase activity. Also, we were interested in investigating the mechanism by which PON1 could be inactivated and the correlation between its enzymatic activity and the antioxidant properties of HDL. Three different oxidation systems were ...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/1071576021000036614

    authors: Jaouad L,Milochevitch C,Khalil A

    更新日期:2003-01-01 00:00:00

  • TNF alpha alters mitochondrial membrane potential in L929 but not in TNF alpha-resistant L929.12 cells: relationship with the expression of stress proteins, annexin 1 and superoxide dismutase activity.

    abstract::Tumour necrosis factor alpha (TNF alpha) cytotoxicity is mediated, at least in part, by oxidative stress and phospholipase A2 activation. The first post-receptor events to be observed in TNF alpha-sensitive lines are the generation of superoxide anion (O2-) within the mitochondria and the activation of phospholipase A...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715769609149917

    authors: Polla BS,Jacquier-Sarlin MR,Kantengwa S,Mariéthoz E,Hennet T,Russo-Marie F,Cossarizza A

    更新日期:1996-08-01 00:00:00

  • Effect of methylguanidine in a model of septic shock induced by LPS.

    abstract::Septic shock, a severe form of sepsis, is characterized by cardiovascular collapse following microbial invasion of the body. The progressive hypotension, hyporeactivity to vasopressor agents and vascular leak leads to circulatory failure with multiple organ dysfunction and death. Many inflammatory mediators (e.g. TNF-...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760410001725517

    authors: Marzocco S,Di Paola R,Ribecco MT,Sorrentino R,Domenico B,Genesio M,Pinto A,Autore G,Cuzzocrea S

    更新日期:2004-11-01 00:00:00

  • Curcumin ameliorates streptozotocin-induced liver damage through modulation of endoplasmic reticulum stress-mediated apoptosis in diabetic rats.

    abstract::We investigated the effect of curcumin on liver injury in diabetic rats induced by streptozotocin (STZ) through modulation of endoplasmic reticulum stress (ERS) and unfolded protein response (UPR). Experimental diabetes was induced by a single intraperitoneal injection of STZ (55 mg/kg), and curcumin was given at 100 ...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715762.2014.999674

    authors: Afrin R,Arumugam S,Soetikno V,Thandavarayan RA,Pitchaimani V,Karuppagounder V,Sreedhar R,Harima M,Suzuki H,Miyashita S,Nomoto M,Suzuki K,Watanabe K

    更新日期:2015-03-01 00:00:00

  • Curcumin induces apoptosis in human neuroblastoma cells via inhibition of AKT and Foxo3a nuclear translocation.

    abstract::Neuroblastoma (NB) is one of the most frequent extracranial solid tumors in children. It accounts for 8-10% of all childhood cancer deaths, and there is a need for development of new drugs for its treatment. Curcumin (diferuloylmethane), a major active component of turmeric (Curcuma longa), has been shown to exert ant...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715762.2014.960410

    authors: Picone P,Nuzzo D,Caruana L,Messina E,Scafidi V,Di Carlo M

    更新日期:2014-12-01 00:00:00