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Structural basis of ribosomal RNA transcription regulation.

Abstract：

:Ribosomal RNA (rRNA) is most highly expressed in rapidly growing bacteria and is drastically downregulated under stress conditions by the global transcriptional regulator DksA and the alarmone ppGpp. Here, we determined cryo-electron microscopy structures of the Escherichia coli RNA polymerase (RNAP) σ70 holoenzyme during rRNA promoter recognition with and without DksA/ppGpp. RNAP contacts the UP element using dimerized α subunit carboxyl-terminal domains and scrunches the template DNA with the σ finger and β' lid to select the transcription start site favorable for rapid promoter escape. Promoter binding induces conformational change of σ domain 2 that opens a gate for DNA loading and ejects σ1.1 from the RNAP cleft to facilitate open complex formation. DksA/ppGpp binding also opens the DNA loading gate, which is not coupled to σ1.1 ejection and impedes open complex formation. These results provide a molecular basis for the exceptionally active rRNA transcription and its vulnerability to DksA/ppGpp.

journal_name

Nat Commun

journal_title

Nature communications

authors

Shin Y,Qayyum MZ,Pupov D,Esyunina D,Kulbachinskiy A,Murakami KS

doi

10.1038/s41467-020-20776-y

subject

Has Abstract

pub_date

2021-01-22 00:00:00

pages

528

issue

1

issn

2041-1723

pii

10.1038/s41467-020-20776-y

journal_volume

12

pub_type

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