Abstract:
:The human genome is persistently exposed to damage caused by xenobiotics, therefore the assessment of genotoxicity of substances having a direct contact with humans is of importance. Phthalates are commonly used in industrial applications. Widespread exposure to phthalates has been evidenced by their presence in human body fluids. We have assessed the genotoxic potential of selected phthalates and mechanism of their action in human peripheral blood mononuclear cells (PBMCs). Studied cells were incubated with di-n-butyl phthalate (DBP), butylbenzyl phthalate (BBP) and their metabolites: mono-n-butylphthalate (MBP), mono-benzylphthalate (MBzP) in the concentrations range of 0.1-10 µg/mL for 24 h. Analyzed compounds induced DNA single and double strand-breaks (DBP and BBP ≥ 0.5 µg/mL, MBP and MBzP ≥ 1 µg/mL) and more strongly oxidized purines than pyrimidines. None of the compounds examined was capable of creating adducts with DNA. All studied phthalates caused an increase of total ROS level, while hydroxyl radical was generated mostly by DBP and BBP. PBMCs exposed to DBP and BBP could not completely repair DNA strand-breaks during 120 min of postincubation, in opposite to damage caused by their metabolites, MBP and MBzP. We have concluded that parent phthalates: DBP and BBP caused more pronounced DNA damage compared to their metabolites.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Sicińska P,Mokra K,Wozniak K,Michałowicz J,Bukowska Bdoi
10.1038/s41598-020-79932-5subject
Has Abstractpub_date
2021-01-18 00:00:00pages
1658issue
1issn
2045-2322pii
10.1038/s41598-020-79932-5journal_volume
11pub_type
杂志文章abstract::The new-type electrolysis-integrated ecological floating beds (EEFBs) were set up to study their water removal ability due to the excellent water treatment capacity of electrolysis, this enhanced EEFBs were made of polyethylene filled with biochar substrate and in middle of the substrate placed the Mg-Al alloy served ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64499-y
更新日期:2020-05-06 00:00:00
abstract::Climate change is the greatest threat to coral reef ecosystems. In particular, increasing ocean temperatures are causing severe and widespread coral bleaching, contributing to extensive coral loss and degradation of coral reef habitats globally. Effects of coral bleaching are not however, equally apportioned among dif...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-44809-9
更新日期:2019-06-10 00:00:00
abstract::The recent discovery of magnetic skyrmion lattices initiated a surge of interest in the scientific community. Several novel phenomena have been shown to emerge from the interaction of conducting electrons with the skyrmion lattice, such as a topological Hall-effect and a spin-transfer torque at ultra-low current densi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21347
更新日期:2016-02-19 00:00:00
abstract::The estrogenicity of parabens at human exposure levels has become a focus of concern due to the debate over whether the estrogenicity of parabens is strong enough to play a role in the increased incidence of breast cancer. In this study, the uterotrophic activities of methylparaben (MP) and ethylparaben (EP) at doses ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25173
更新日期:2016-04-28 00:00:00
abstract::Cortical spreading depolarisation (CSD), the neural mechanism underlying migraine aura, may cause headache by sensitising the trigeminal system. Photophobia, the most bothersome accompanying symptom during migraine attacks, is more prevalent in migraine with aura than in migraine without aura. Whether CSD plays a role...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67948-w
更新日期:2020-07-10 00:00:00
abstract::In order to study the molecular differences between type 2 diabetes mellitus (T2DM) and T2DM with depression (DD), we aimed to screen the differential expression of lncRNA, mRNA, and circRNA in the blood of patients with T2DM and DD. Based on the self-rating depression scale (SDS), patient health questionnaire 9 (PHQ9...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47077-9
更新日期:2019-07-24 00:00:00
abstract::Abnormalities in actin cytoskeleton have been linked to Friedreich's ataxia (FRDA), an inherited peripheral neuropathy characterised by an early loss of neurons in dorsal root ganglia (DRG) among other clinical symptoms. Despite all efforts to date, we still do not fully understand the molecular events that contribute...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62050-7
更新日期:2020-03-23 00:00:00
abstract::This study was initiated to improve E. coli succinate production by engineering the E. coli global transcription factor, Cra (catabolite repressor/activator). Random mutagenesis libraries were generated through error-prone PCR of cra. After re-screening and mutation site integration, the best mutant strain was Tang154...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36526
更新日期:2016-11-04 00:00:00
abstract::Regular exercise elicits advantageous metabolic adaptations in skeletal muscle, such as improved insulin sensitivity. However, the underpinning molecular mechanisms and the effect of diet on muscle exercise training benefits are unclear. We therefore characterized the skeletal muscle proteome following exercise traini...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-28540-5
更新日期:2018-07-16 00:00:00
abstract::Short term treatment with low doses of glucocorticoid analogues has been shown to ameliorate neurological symptoms in Ataxia-Telangiectasia (A-T), a rare autosomal recessive multisystem disease that mainly affects the cerebellum, immune system, and lungs. Molecular mechanisms underlying this clinical observation are u...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77352-z
更新日期:2020-11-19 00:00:00
abstract::The potential of a spheroid tumor model composed of cells in different proliferative and metabolic states for the development of new anticancer strategies has been amply demonstrated. However, there is little or no information in the literature on the problems of reproducibility of data originating from experiments us...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19103
更新日期:2016-01-11 00:00:00
abstract::The aim of this study was to investigate the correlation between elastography and histologic characteristics including fibrosis and calcification. We also wanted to investigate whether other clinicopathologic indexes influence the strain ratio (SR) of papillary thyroid carcinomas (PTCs). We retrospectively reviewed 12...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45042
更新日期:2017-03-22 00:00:00
abstract::The primary motor cortex (M1) is highly influenced by premotor/motor areas both within and across hemispheres. Dual site transcranial magnetic stimulation (dsTMS) has revealed interhemispheric interactions mainly at early latencies. Here, we used dsTMS to systematically investigate long-latency causal interactions bet...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13708-2
更新日期:2017-11-02 00:00:00
abstract::Infestation by the biotrophic pathogen Gymnosporangium asiaticum can be devastating for plant of the family Rosaceae. However, the phytopathology of this process has not been thoroughly elucidated. Using a metabolomics approach, we discovered the intrinsic activities that induce disease symptoms after fungal invasion ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37434
更新日期:2016-11-28 00:00:00
abstract::Src homology 2 domain-containing protein tyrosine phosphatase 2 (SHP-2) is a cytoplasmic tyrosine phosphatase that is highly expressed in hematopoietic cells and in the CNS and exerts opposite effects on signal transduction by exerting a neuroprotective or proapoptotic effect. Several mutations of SHP-2 have been foun...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06401-x
更新日期:2017-07-20 00:00:00
abstract::Cells harboring latent HIV-1 pose a major obstacle to eradication of the virus. The 'shock and kill' strategy has been broadly explored to purge the latent reservoir; however, none of the current latency-reversing agents (LRAs) can safely and effectively activate the latent virus in patients. In this study, we report ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07157-0
更新日期:2017-08-25 00:00:00
abstract::The taxonomic composition of egg-associated microbial communities can play a crucial role in the development of fish embryos. In response, hosts increasingly influence the composition of their associated microbial communities during embryogenesis, as concluded from recent field studies and laboratory experiments. Howe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17084
更新日期:2015-11-27 00:00:00
abstract::Successful tissue repair requires the activities of myeloid cells such as monocytes and macrophages that guide the progression of inflammation and healing outcome. Immunoregenerative materials leverage the function of endogenous immune cells to orchestrate complex mechanisms of repair; however, a deeper understanding ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00477-1
更新日期:2017-03-27 00:00:00
abstract::During skin healing, periostin facilitates myofibroblast differentiation through a β1 integrin/FAK dependent mechanism and continued expression is associated with scarring. In contrast to skin, gingival tissue does not typically scar upon injury, but the role of periostin in gingival healing has never been investigate...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35805-6
更新日期:2019-02-25 00:00:00
abstract::Refractive surgical treatment of hyperopia still remains a challenge for refractive surgeons. A new nomogram of small incision lenticule extraction (SMILE) procedure has recently been developed for the treatment of hyperopia. In the present study, we aimed to evaluate the wound healing and inflammatory responses of th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29802
更新日期:2016-07-15 00:00:00
abstract::A major translational challenge in the fields of therapeutic angiogenesis and regenerative medicine is the need to create functional microvasculature. The purpose of this study was to assess whether a potentially autologous endothelial cell (EC) source derived from human induced pluripotent stem cells (iPSC-ECs) can f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20966-1
更新日期:2018-02-08 00:00:00
abstract::One of the greatest enigmas of modern biology is how the geometry of muscular and skeletal structures are created and how their development is controlled during growth and regeneration. Scaling and shaping of vertebrate muscles and skeletal elements has always been enigmatic and required an advanced technical level in...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32459-2
更新日期:2018-09-20 00:00:00
abstract::In the research field of extracellular vesicles (EVs), the use of fetal bovine serum (FBS) depleted of EVs for in vitro studies is advocated to eliminate the confounding effects of media derived EVs. EV-depleted FBS may either be prepared by ultracentrifugation or purchased commercially. Nevertheless, these preparatio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41772-3
更新日期:2019-04-02 00:00:00
abstract::The ecological success of diatoms is emphasized by regular blooms of many different species in all aquatic systems, but the reason behind their success is not fully understood. A special feature of the diatom cell is the frustule, a nano-patterned cell encasement made of amorphous biosilica. The optical properties of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17403
更新日期:2015-12-02 00:00:00
abstract::Hedgehog interacting protein (Hhip) is essential for islet formation and beta-cell proliferation during pancreatic development; abnormally elevated Hhip expression has been linked to human pancreatitis. Here, we investigate the role of Hhip in modulating insulin secretion in adult Hhip mice (Hhip +/- vs. Hhip+/+) fed ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47633-3
更新日期:2019-08-01 00:00:00
abstract::The cytoskeletal filament vimentin is inherent to the endothelial phenotype and is critical for the proper function of endothelial cells in adult mice. It is unclear, however, if the presence of vimentin is necessary during differentiation to the endothelial phenotype. Here we evaluated gene and protein expression of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30814
更新日期:2016-08-02 00:00:00
abstract::Aortic stenosis (AS) is the most common form of valve disease. Once symptoms develop, there is an inexorable deterioration with a poor prognosis; currently there are no therapies capable of modifying disease progression, and aortic valve replacement is the only available treatment. Our goal is to study the progression...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27106
更新日期:2016-06-03 00:00:00
abstract::Migraine headache is often timed with the menstrual cycle. Some studies have reported reduced risk of breast cancer in migraineurs but most of those did not distinguish menstrually-related from non-menstrually-related migraine. To examine the possible associations between breast cancer and migraine overall and between...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14943
更新日期:2015-10-12 00:00:00
abstract::Boolean networks are widely used to model gene regulatory networks and to design therapeutic intervention strategies to affect the long-term behavior of systems. In this paper, we investigate the less-studied one-bit perturbation, which falls under the category of structural intervention. Previous works focused on fin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26247
更新日期:2016-05-19 00:00:00
abstract::Ischemic neural damages cause several devastating diseases, including brain stroke and ischemic retinopathies, and endoplasmic reticulum (ER) stress has been proposed to be the underlying mechanism of the neuronal cell death of these conditions. We previously synthesized Kyoto University substances (KUSs) as modulator...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44873
更新日期:2017-03-20 00:00:00