Abstract:
RESEARCH QUESTION:Granulosa cells (GCs) surrounding oocytes are crucial for follicular growth, oocyte development, ovulation, and luteinization under the dynamic co-stimulation of follicle stimulating hormone (FSH) and luteinizing hormone (LH). This study aimed to investigate the effect of LH levels on GCs in preovulatory follicles under gonadotropin releasing hormone antagonist-based ovarian stimulation. In vitro experiments were also conducted to study the direct effect of LH on GCs. METHODS:Twelve infertile women were divided into low (L), medium (M), and high (H) LH groups according to their serum LH levels during ovarian stimulation. RNA-sequencing (RNA-seq) was conducted to examine the transcriptome profiles of GCs obtained from the above patients during the oocyte retrieval. The activity of mitochondrial dehydrogenase was measured under the stimulation of recombinant LH (rLH) concentration gradient combined with recombinant FSH. The ultrastructures of subcellular organelles were observed. RESULTS:Bioinformatic analyses showed that compared with the M group, molecule and pathway changes in the L group and in the H group were similar. In cultured GCs, both insufficient and excessive rLH impaired the activity of mitochondrial dehydrogenase. With the medium rLH concentration, numerous cell connections and abundant mitochondria and liposomes were observed. Compared with the medium concentration, GCs showed smaller and rounder mitochondria, more autophagosomes, and massive organelles damages with excessive rLH, and swollen, circular, or forked mitochondria were observed with inadequate rLH. CONCLUSIONS:RNA-seq provided a novel spectrum of transcriptome characteristics of GCs potentially affected by serum LH levels during ovarian stimulation. In vitro, rLH could directly affect GCs at the subcellular level.
journal_name
J Assist Reprod Genetjournal_title
Journal of assisted reproduction and geneticsauthors
Wan YT,Liu S,Zhao SK,Luo YY,Lv YS,Qu DN,Liu MH,Li Ydoi
10.1007/s10815-021-02066-8subject
Has Abstractpub_date
2021-01-14 00:00:00eissn
1058-0468issn
1573-7330pii
10.1007/s10815-021-02066-8pub_type
杂志文章abstract::The subject is first identified in terms of the new understanding of a pre-embryonic phase preceding the traditional embryonic and fetal periods of formation and development. Sample fields and purposes of pre-embryonic research are instanced, some with direct therapeutic intent, others with foreseeable but not immedia...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/BF02212914
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journal_title:Journal of assisted reproduction and genetics
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journal_title:Journal of assisted reproduction and genetics
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doi:10.1007/s10815-010-9453-0
更新日期:2010-11-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
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journal_title:Journal of assisted reproduction and genetics
pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
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更新日期:1996-03-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-013-0031-0
更新日期:2013-07-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章,评审
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更新日期:2018-06-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章,meta分析
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更新日期:2006-01-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
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更新日期:2010-09-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-018-1350-y
更新日期:2019-02-01 00:00:00
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更新日期:2017-02-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
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journal_title:Journal of assisted reproduction and genetics
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pub_type: 杂志文章
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journal_title:Journal of assisted reproduction and genetics
pub_type: 历史文章,杂志文章,评审
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更新日期:2013-08-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-012-9801-3
更新日期:2012-09-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-018-1187-4
更新日期:2018-08-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-019-01573-z
更新日期:2019-11-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1023/a:1009445913156
更新日期:2000-01-01 00:00:00
abstract::In an attempt to assess the effect of perfluorinated compounds (PFC) on oocytes quality and fertilization rate, we studied follicular fluid (FF) PFC levels in 18 patients undergoing IVF-ET cycles. A significant correlation (R = 0.75; P < 0.001) was observed between FF PFC levels and fertilization rate. Moreover, patie...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-011-9548-2
更新日期:2011-05-01 00:00:00
abstract:PURPOSE:This study aimed to induce follicular wave emergence (FWE) using pharmacological (recombinant hCG administration) or mechanical (aspiration of dominant follicle) interventions in infertile women. METHODS:Sixteen infertile women (≤35 years) with indications for in vitro fertilization due to tubal and/or male fa...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10815-015-0432-3
更新日期:2015-04-01 00:00:00
abstract::We found no difference in clinical pregnancy rates following IVF-ET in women with anteverted versus retroverted uteri. Patients can be reassured that the probability of clinical pregnancy following IVF-ET is not compromised by uterine position. ...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/BF02215722
更新日期:1994-07-01 00:00:00
abstract:PURPOSE:Previous studies reported that patients with endometriosis had excess nitric oxide (NO) in the reproductive tract and poor embryo development in IVF cycles. This study aims to elucidate the effects of NO on early embryo development. METHODS:Zygotes from superovulated B6CBF1 mice were cultured to blastocysts in...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-013-0045-7
更新日期:2013-08-01 00:00:00
abstract:PURPOSE:To determine the percentage of unbalanced spermatozoa and an interchromosomal effect in two carriers of balanced translocations t(13;15)(q32;q26) and t(13;15)(q32;p11.2). METHODS:Sperm nuclei analysis by fluorescent in situ hybridization for detection of percentage of unbalanced spermatozoa and sperm with diso...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-012-9767-1
更新日期:2012-07-01 00:00:00