Abstract:
:Toxic and volatile chemicals are widely used in household products and previously used as warfare agents, causing a public health threat worldwide. This study aimed to evaluate the extent of injury and mechanisms of acrolein toxicity in the cornea. Primary human corneal stromal fibroblasts cultures (hCSFs) from human donor cornea were cultured and exposed to acrolein toxicity with -/+ N-acetylcysteine (NAC) to study the mode of action in the presence of Buthionine sulphoximine (BSO). PrestoBlue and MTT assays were used to optimize acrolein, NAC, and BSO doses for hCSFs. Cell-based assays and qRT-PCR analyses were performed to understand the acrolein toxicity and mechanisms. Acrolein exposure leads to an increased reactive oxygen species (ROS), compromised glutathione (GSH) levels, and mitochondrial dysfunction. The TUNEL and caspase assays showed that acrolein caused cell death in hCSFs. These deleterious effects can be mitigated using NAC in hCSFs, suggesting that GSH can be a potential target for acrolein toxicity in the cornea.
journal_name
Toxicol Lettjournal_title
Toxicology lettersauthors
Gupta S,Kamil S,Sinha PR,Rodier JT,Chaurasia SS,Mohan RRdoi
10.1016/j.toxlet.2021.01.005subject
Has Abstractpub_date
2021-01-06 00:00:00pages
33-42eissn
0378-4274issn
1879-3169pii
S0378-4274(21)00006-0journal_volume
340pub_type
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