Abstract:
:Circadian rhythm is well known to play a pivotal role in reproduction but the presence of a gonadal circadian rhythm is opening a lot of questions about a local regulation of reproduction. In the present study, we first set to identify the key genes driving circadian rhythmicity from the gonadal transcriptome of the swordfish, a commercially relevant species undergoing overfishing, and we then investigated whether their transcriptional activity was influenced by the maturation stage. Finally, we explored whether seasonality had the ability to modulate the expression of these genes. Interestingly, we identified a wide number of circadian rhythm related genes in the transcriptome of the swordfish gonad including, among the others, clock, bmal1, cry2 and per2, which have been found to be differentially expressed between sexually immature and mature individuals sampled during the breeding season. This differential modulation was also found for melatonin biosynthesis genes (mel1b, asmt and tph1) as well as opsin genes (opsin4, tmt opsin, parapinopsin, VA opsin, rho and sws), known to be the primary receptors of light stimuli. These differences were not observed between mature and immature individuals when considering the non-breeding season suggesting that, despite the molecular machinery of mature gonads is able to respond to signals driving ovarian maturation, these signals are not present hence highlighting the potential role of seasonality in modulating the gonadal circadian rhythm. These results confirm the presence of a gonadal circadian rhythm in the swordfish and open new interesting questions about its role in driving puberty onset.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Basili D,Gioacchini G,Todisco V,Candelma M,Marisaldi L,Pappalardo L,Carnevali Odoi
10.1016/j.ygcen.2020.113707subject
Has Abstractpub_date
2020-12-30 00:00:00pages
113707eissn
0016-6480issn
1095-6840pii
S0016-6480(20)30460-3journal_volume
303pub_type
杂志文章abstract::Somatostatin receptors (sst) mediate the numerous physiological actions (e.g., aspects of growth, development, and metabolism) of the somatostatin family of peptides. In this study, we used rainbow trout (Oncorhynchus mykiss) to establish the pattern of sst subtype 1A, 1B, and 2 mRNA expression in selected tissues (op...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2004.09.008
更新日期:2004-12-01 00:00:00
abstract::The Remipedia is a small, recently described crustacean class that inhabits submerged marine/anchialine cave systems. Phylogenetic and morphological investigations support a sister group relationship between these animals and the hexapods. The recent deposition of numerous (>100,000) transcriptome shotgun assembly seq...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2014.01.017
更新日期:2014-05-15 00:00:00
abstract::The effects of salmon gonadotropin-releasing hormone (sGnRH) and the superactive agonist [D-Arg6, Pro9NEt]-sGnRH (sGnRH-A) on growth hormone (GH) and gonadotropin (GtH) release were examined using a perifusion system for pituitary fragments of the common carp (Cyprinus carpio). Perifusion of 2-min pulses of different ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1993.1009
更新日期:1993-01-01 00:00:00
abstract::Steroid hormone assessment using non-invasive sample collection techniques can reveal the reproductive status of aquatic mammals and the physiological mechanisms by which they respond to changes in their environment. A portion of the eastern North Pacific blue whale (Balaenoptera musculus) population that seasonally v...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2018.02.015
更新日期:2018-05-15 00:00:00
abstract::Profiles of testosterone, 11-ketotestosterone, androstenedione, and estradiol were determined by RIA, and immunocytochemical techniques were employed to identify gonadotropin (GTH) I and II and gonadotropin releasing hormone (GnRH) in monosex and mixed sex populations of rainbow trout from 1 to 126 days postfertilizat...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1996.0083
更新日期:1996-06-01 00:00:00
abstract::We have studied the effects of bovine parathyroid hormone (PTH) and salmon calcitonin (CT) on plasma calcium (Ca) and phosphate levels and on renal function in intact and parathyroidectomized (PTX) chickens, using a simple technique that separates urine from feces. In immature males and egg-laying females, the intrave...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(87)90248-6
更新日期:1987-06-01 00:00:00
abstract::Twelve male edible dormice, captured in the autumn of 1979, were studied for 2 years under natural lighting in an animal enclosure with permanently open windows, at Montpellier, in Southern France (latitude 43 degrees 50' N). Ambient temperature fluctuated annually between +4 and 27 degrees, and the photoperiod betwee...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(83)90266-6
更新日期:1983-06-01 00:00:00
abstract::Arsenic contaminates water surface and groundwater worldwide. Several studies have suggested that arsenic acts as an endocrine disruptor in mammalian and non-mammalian species, although its chronic effect during development remains largely unknown. To address this question, life cycle exposures to 0, 0.3 and 0.8ppm of...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2015.09.015
更新日期:2016-08-01 00:00:00
abstract::Since an extrapancreatic source of insulin has been postulated in the chick embryo, insulin was sought by immunohistochemistry in the embryo before and after development of the pancreas and other tissues. Using for the first time an antiserum specific for chicken insulin, localization of insulin was investigated in wh...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(89)90102-0
更新日期:1989-02-01 00:00:00
abstract::This study compares follicular function and ovulatory efficiency in 20 sows with obesity/leptin resistance genotype (Iberian pig) and 20 females of lean commercial crosses (Large White × Landrace; LW×L). Estrous cycle was synchronized with progestagens; ovulation was induced with eCG and hCG, in half of the females of...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2010.10.006
更新日期:2011-01-01 00:00:00
abstract::Melatonin binding sites were examined in the quail eye using 2-[125]iodomelatonin. Radioreceptor assays indicated similar binding sites in membrane preparations of neural retina (NR) and choroid-retinal pigment epithelium (C-RPE) eye components. In both tissues binding of the radioligand was specific, saturable, and o...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1995.1148
更新日期:1995-11-01 00:00:00
abstract::An investigation into vitamin D-like molecules has been performed on whole extracts of the terrestrial amphipod Orchestia cavimana, using a sensitive nonequilibrium assay employing 1,25-(OH)2 D receptor from calf thymus. Relatively large amounts of these secosteroid-like molecules were observed and they varied in conc...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(91)90070-m
更新日期:1991-10-01 00:00:00
abstract::Previous reports have shown that adenosine in rat inhibits both spontaneous and ACTH-induced release of corticosteroids through activation of adenosine A1 receptors. In the present study, we have investigated the possible effect of adenosine in the secretion of corticosteroids in amphibians using a perfusion technique...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(92)90070-z
更新日期:1992-06-01 00:00:00
abstract::In the present study, four full-length cDNAs of somatostatin receptor (sstr) were cloned from the forebrain and pituitary of red-spotted grouper. The four full-length cDNAs were designated 2292, 1522, 1873 and 1789 bp and identified as sstr1, sstr2, sstr3, and sstr5 by BLAST analysis; the corresponding sizes of the op...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2019.01.007
更新日期:2019-04-01 00:00:00
abstract::The neuroactive steroids which are synthesized in the brain and nervous system are known as "Neurosteroids". These steroids have crucial functions such as contributing to the myelination and organization of the brain connectivity. Under the stressful circumstances, the concentrations of neurosteroid products such as a...
journal_title:General and comparative endocrinology
pub_type: 杂志文章,评审
doi:10.1016/j.ygcen.2015.09.030
更新日期:2016-01-01 00:00:00
abstract::Hormones play key, functional roles in mediating the tradeoff between survival and reproduction. Glucocorticoid hormones can inhibit reproduction and improve chances of survival during periods of stress. However, glucocorticoid hormones are, at times, also associated with successfully engaging in energetically costly ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2014.08.008
更新日期:2014-11-01 00:00:00
abstract::Insulin (INS) plays a critical role in the growth, development, and metabolism of vertebrates. In this study, a cDNA encoding a novel insulin receptor (IR) subtype was isolated, cloned, and sequenced from the liver of rainbow trout. A 1525-bp cDNA encoding a partial amino acid sequence of the β-subunit including the t...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2010.09.005
更新日期:2010-12-01 00:00:00
abstract::Stress is a known inhibitor of reproductive function. The mechanisms by which stress acts to influence the reproductive axis have been intensely studied and appear to be extremely varied. Gonadotropin-releasing hormone (GnRH) is a critical component of the vertebrate reproductive axis and directly causes pituitary gon...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2008.05.010
更新日期:2008-07-01 00:00:00
abstract::The corticosterone response to the sight of a natural predator was investigated in free-living and captive great tits (Parus major). Free-living great tits responded to the sight of a stuffed, slowly moving Tengmalm's owl, a major predator of great tits, with warning calls and a change in behaviour around a feeder. Gr...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.2001.7749
更新日期:2002-02-01 00:00:00
abstract::This set of experiments investigated the role of protein kinase-C (PKC) as a second messenger in vasoactive intestinal peptide (VIP)-stimulated prolactin (PRL) secretion and PRL mRNA abundance. Dispersed anterior pituitary cells (5 x 10(5) or 10(6) cells/tube) were isolated from laying turkeys and incubated in 1.0 ml ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1995.1112
更新日期:1995-09-01 00:00:00
abstract::Adrenomedullin (AM) is a multifunctional evolutionarily highly conserved peptide. Although its genomic and amino acid (aa) sequences are known in several mammalian species and in fish, the structure of the AM gene remains unknown in intermediate phyla, including birds. Here, we report the structure and aa sequence of ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2005.02.014
更新日期:2005-08-01 00:00:00
abstract::Hypothalamic kisspeptin plays a pivotal role in the regulation of the hypothalamic-pituitary-gonadal axis by stimulating gonadotropin-releasing hormone (GnRH) release into the portal circulation, with the subsequent release of gonadotropins. Kisspeptin and its receptor, the kisspeptin 1 receptor (Kiss1R), are also exp...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2017.01.014
更新日期:2017-05-15 00:00:00
abstract::Interference in radioimmunoassays (RIAs) was frequently encountered during endocrinological studies of the emu (Dromaius novaehollandiae). Interference was greatest when serum was cloudy or opaque. Such samples appeared seasonally, in spring and summer during the phase of fat deposition, and in the winter when females...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/s0016-6480(03)00088-1
更新日期:2003-06-15 00:00:00
abstract::Day length predicts changing of seasons at mid-latitudes, but additional environmental cues (e.g., temperature, rainfall) give more precise information about timing of food peaks critical for offspring survival. We tested the effects of temperature on seasonal reproductive development of male song sparrows (Melospiza ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2005.03.004
更新日期:2005-09-01 00:00:00
abstract::In anaesthetized young (6 weeks old) and adult (22-30 weeks old) domestic fowl, the administration of thyrotrophin releasing hormone (TRH; 1.0 micrograms/kg in young birds; 10.0 micrograms/kg in adults) or human pancreatic growth hormone-releasing factor (hpGRF(1-44)NH2; 10.0 micrograms/kg in both cases) markedly incr...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(85)90413-7
更新日期:1985-07-01 00:00:00
abstract::This article is focused on the effect of specific phase relation of serotonergic and dopaminergic oscillations on the gonadal responses of different avian species. These species include (i) summer breeding birds - Red headed bunting (exhibiting post-reproductive absolute photorefractoriness) and Indian Weaver bird (wh...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2013.06.019
更新日期:2013-09-01 00:00:00
abstract::Secretin (Sct), traditionally a gastrointestinal hormone backed by a century long research, is now beginning to be recognized also as a neuroactive peptide. Substantiation by recent evidence on the functional role of Sct in various regions of the brain, especially on its potential neurosecretion from the posterior pit...
journal_title:General and comparative endocrinology
pub_type: 杂志文章,评审
doi:10.1016/j.ygcen.2012.11.017
更新日期:2013-01-15 00:00:00
abstract::In captivity, the adrenocortical stress response can be permanently altered by events that occur during early life. Free-living animals have rarely been examined in this regard. To examine whether early-life events impact the hypothalamo-pituitary-adrenal (HPA) axis in the natural setting, we evaluated the stress resp...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2011.08.022
更新日期:2011-11-01 00:00:00
abstract::The dynamics of androgen production by perfused Xenopus testis explants has been investigated under various gonadotropic stimulations. Androgens were measured in perfusate by radioimmunoassay. The behavior of hormones in the perfusion system was assessed. Pieces of testis were capable of producing androgens after more...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(83)90057-6
更新日期:1983-09-01 00:00:00
abstract::The response of plasma prolactin (PRL) to manipulations of plasma calcium were investigated in coho salmon. Injection of 3.16 mumol EGTA/10 g body weight (to reduce the plasma calcium activity) resulted in significantly higher plasma PRL levels. Lower doses of EGTA (1.06 and 2.13 mumol/10 g body wt) had no effect on p...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(89)90197-4
更新日期:1989-03-01 00:00:00