Abstract:
:Microbial induced calcite precipitation (MICP) based on ureolysis has a high potential for many applications, e.g. restoration of construction materials. The gram-positive bacterium Sporosarcina pasteurii is the most commonly used microorganism for MICP due to its high ureolytic activity. However, Sporosarcina pasteurii is so far cultivated almost exclusively in complex media, which only results in moderate biomass concentrations at the best. Cultivation of Sporosarcina pasteurii must be strongly improved in order to make technological application of MICP economically feasible. The growth of Sporosarcina pasteurii DSM 33 was boosted by detecting auxotrophic deficiencies (L-methionine, L-cysteine, thiamine, nicotinic acid), nutritional requirements (phosphate, trace elements) and useful carbon sources (glucose, maltose, lactose, fructose, sucrose, acetate, L-proline, L-alanine). These were determined by microplate cultivations with online monitoring of biomass in a chemically defined medium and systematically omitting or substituting medium components. Persisting growth limitations were also detected, allowing further improvement of the chemically defined medium by the addition of glutamate group amino acids. Common complex media based on peptone and yeast extract were supplemented based on these findings. Optical density at the end of each cultivation of the improved peptone and yeast extract media roughly increased fivefold respectively. A maximum OD600 of 26.6 ± 0.7 (CDW: 17.1 ± 0.5 g/L) was reached with the improved yeast extract medium. Finally, culture performance and media improvement was analysed by measuring the oxygen transfer rate as well as the backscatter during shake flask cultivation.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Lapierre FM,Schmid J,Ederer B,Ihling N,Büchs J,Huber Rdoi
10.1038/s41598-020-79904-9subject
Has Abstractpub_date
2020-12-31 00:00:00pages
22448issue
1issn
2045-2322pii
10.1038/s41598-020-79904-9journal_volume
10pub_type
杂志文章abstract::Non-alcoholic steatohepatitis (NASH) is a progressive form of non-alcoholic fatty liver disease (NAFLD) that may lead to liver cirrhosis or hepatocellular carcinoma. Here, we examined the diagnostic utility of tri-antennary tri-sialylated mono-fucosylated glycan of alpha-1 antitrypsin (AAT-A3F), a non-invasive glycobi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56947-1
更新日期:2020-01-15 00:00:00
abstract::In order to make full use of the impact of internal and external factors on the performance of title catalyst for ethyl benzene oxidation, the key internal influencing factors on the catalytic performance were modulated by coordinating and grafting manganese porphyrin to mesoporous and macroporous chitosan, and the im...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-70210-y
更新日期:2020-08-24 00:00:00
abstract::Aldehyde dehydrogenase (ALDH) is a key enzyme for the catalytic oxidation of acetaldehyde to acetic acid. Genetic polymorphisms of ALDH2 have been associated with a wide range of diseases and cancers. However, little information is found about the association between ALDH2 polymorphisms and lumbar disc herniation (LDH...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31491-6
更新日期:2018-08-30 00:00:00
abstract::LiYF4:Eu nanophosphors with a single tetragonal phase are synthesized, and various strategies to enhance the Eu(3+) emission from the nanophosphors are investigated. The optimized Eu(3+) concentration is 35 mol%, and the red emission peaks due to the (5)D0 →(7)FJ (J = 1 and 2) transitions of Eu(3+) ions are further en...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07866
更新日期:2015-01-19 00:00:00
abstract::Chemotherapy (CTx)-induced premature ovarian failure (POF) in woman remains clinically irreversible. Amniotic fluid stem cells (AFSCs) have shown the potential to treat CTx-induced POF; however, the underlying mechanism is unclear. Here we demonstrate that AFSC-derived exosomes recapitulate the anti-apoptotic effect o...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23120
更新日期:2016-03-16 00:00:00
abstract::Geographical variation of diabetic emergencies attended by prehospital emergency medical services (EMS) and the relationship between area-level social and demographic factors and risk of a diabetic emergency were examined. All cases of hypoglycaemia and hyperglycaemia attended by Ambulance Victoria between 1/01/2009 a...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1038/s41598-018-23457-5
更新日期:2018-03-23 00:00:00
abstract::Realization of phosphor-free white-light emitters is becoming an important milestone on the road to achieve high quality and reliability in high-power white-light-emitting diodes (LEDs). However, most of reported methods have not been applied to practical use because of their difficulties and complexity. In this study...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17372
更新日期:2015-12-02 00:00:00
abstract::For nearly a century, researchers have tried to understand the swimming of aquatic animals in terms of a balance between the forward thrust from swimming movements and drag on the body. Prior approaches have failed to provide a separation of these two forces for undulatory swimmers such as lamprey and eels, where most...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07329
更新日期:2014-12-10 00:00:00
abstract::During plaque progression, inflammatory cells progressively accumulate in the adventitia, paralleled by an increased presence of leaky vasa vasorum. We here show that next to vasa vasorum, also the adventitial lymphatic capillary bed is expanding during plaque development in humans and mouse models of atherosclerosis....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45263
更新日期:2017-03-28 00:00:00
abstract::The recent emergence of highly virulent human adenoviruses (HAdVs) with new tissue tropisms underscores the need to determine their ontogeny. Here we report complete high quality genome sequences and analyses for all the previously unsequenced HAdV serotypes (n = 20) within HAdV species D. Analysis of nucleotide seque...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01812
更新日期:2013-01-01 00:00:00
abstract::Hetero-nanostructures based on magnetic contrast oxides have been prepared as highly dense nanoconsolidates. Cobalt ferrite-cobalt oxide core-shell type nanoparticles (NPs) were synthesized by seed mediated growth in polyol and subsequently consolidated by Spark Plasma Sintering (SPS) at 500 °C for a few minutes while...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55649-y
更新日期:2019-12-19 00:00:00
abstract::The present study aimed to develop a plate-screening method, based on the specific color development of complexes formed between chlorogenic acid, a valuable plant-derived compound, and aluminum (III), to detect chlorogenic acid-producing microbial strains. Modified media with 0.75 mM aluminum chloride were developed ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32968-0
更新日期:2018-10-03 00:00:00
abstract::We developed a virus-like particle (VLP)-based therapeutic vaccine against angiotensin II receptor type 1, ATR-AP205-001, which could significantly reduce the blood pressure and protect target organs of hypertensive animals. In this study, we focused on the immunological effect and safety of the VLP-based vaccine. By ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12996-y
更新日期:2017-10-03 00:00:00
abstract::The ability of radial glial progenitors (RGPs) to generate cortical neurons is determined by local extracellular factors and signaling pathways intrinsic to RGPs. Here we find that GPR157, an orphan G protein-coupled receptor, localizes to RGPs' primary cilia exposed to the cerebrospinal fluid (CSF). GPR157 couples wi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25180
更新日期:2016-05-04 00:00:00
abstract::Human pluripotent stem cells (hPSCs) offer tremendous promise in tissue engineering and cell-based therapies due to their unique combination of two properties: pluripotency and unlimited proliferative capacity. However, directed differentiation of hPSCs to clinically relevant cell lineages is needed to achieve the goa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01684-6
更新日期:2017-05-08 00:00:00
abstract::We investigated the effect of vacancy formation on brittle (D022) to ductile (L12-like) transition in Al3Ti using DFT calculations. The well-known pseudogap on the density of states of Al3Ti migrates towards its Fermi level from far above, via a W - M co-doping strategy, where M is Si, Ge, Sn or Pb respectively. In pa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14398-6
更新日期:2017-10-25 00:00:00
abstract::Grapevine is an important and extensively grown fruit crop, which is severely hampered by drought worldwide. So, comprehending the impact of drought on grapevine genetic resources is necessary. In the present study, RNA-sequencing was executed using cDNA libraries constructed from both drought-stress and control plant...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13464-3
更新日期:2017-10-13 00:00:00
abstract::Glioblastoma (GBM) is the most prevalent and lethal type of primary malignant brain tumour. Recent studies suggest that the discovery of human cytomegalovirus (HCMV)-encoded microRNAs (miRNAs) might play a role in the pathogenesis of diseases, including GBM. In this study, we aimed to analyse the expression and functi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44705
更新日期:2017-03-17 00:00:00
abstract::Interleukin 17 (IL-17) stimulates the osteogenic differentiation of progenitor cells in vitro through a synergy with bone morphogenetic protein (BMP)-2. This study investigates whether the diverse responses mediated by IL-17 in vivo also lead to enhanced BMP-2-induced bone formation. Since IL-17 is known to induce ost...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-25564-9
更新日期:2018-05-08 00:00:00
abstract::The coherent state preparation and control of single quantum systems is an important prerequisite for the implementation of functional quantum devices. Prominent examples for such systems are semiconductor quantum dots, which exhibit a fine structure split single exciton state and a V-type three level structure, given...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep10313
更新日期:2015-05-22 00:00:00
abstract::Drug addiction remains a prevalent and fatal disease worldwide that carries significant social and economic impacts. Recent reports suggest illicit pregabalin (Lyrica) use may be increasing among youth, however the addictive potential of pregabalin has not been well established. Drug seeking behavior and chronic drug ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51556-4
更新日期:2019-10-22 00:00:00
abstract::Odorant binding proteins (OBPs) play critical roles in chemical communication of insects, as they recognize and transport environmental chemical signals to receptors. The diving beetle Cybister japonicus Sharp shows a remarkable sexual dimorphism. The foreleg tarsi of males are equipped with large suction cups, believ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep31848
更新日期:2016-08-22 00:00:00
abstract::We report a low cost and scalable method to synthesize solar selective nanofluids from 'used engine oil'. The as-prepared nanofluids exhibit excellent long-term stability (presently tested up to 6 months under undisturbed stagnant conditions at room temperature) and photo-thermal conversion efficiency. Moreover, these...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47126-3
更新日期:2019-07-22 00:00:00
abstract::Patient-matched transcriptomic studies using tumour samples before and after treatment allow inter-patient heterogeneity to be controlled, but tend not to include an untreated comparison. Here, Illumina BeadArray technology was used to measure dynamic changes in gene expression from thirty-seven paired diagnostic core...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29434
更新日期:2016-07-07 00:00:00
abstract::Impaired immune function contributes to the development of chronic obstructive pulmonary disease (COPD). Disease progression is further exacerbated by pathogen infections due to impaired immune responses. Elimination of infected cells is achieved by cytotoxic CD8(+) T cells that are activated by MHC I-mediated presen...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep10230
更新日期:2015-05-19 00:00:00
abstract::We propose a new method to extend the path length tunability of rotary delay-lines. This method was shown to achieve a duty cycle of >80% and repetition rates of over 40 kHz. The new method relies on a new multi-segmented micro-machined mirror and serial injection of a single reflection onto separate segments of this ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09576-5
更新日期:2017-08-24 00:00:00
abstract::Short QT syndrome (SQTS) is a rare condition characterized by abnormally 'short' QT intervals on the ECG and increased susceptibility to cardiac arrhythmias and sudden death. This simulation study investigated arrhythmia dynamics in multi-scale human ventricle models associated with the SQT2-related V307L KCNQ1 'gain-...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08367-2
更新日期:2017-08-16 00:00:00
abstract::Uncovering complex oil-water flow structure represents a challenge in diverse scientific disciplines. This challenge stimulates us to develop a new distributed conductance sensor for measuring local flow signals at different positions and then propose a novel approach based on multi-frequency complex network to uncove...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08222
更新日期:2015-02-04 00:00:00
abstract::The temporal structure of sound such as in music and speech increases the efficiency of auditory processing by providing listeners with a predictable context. Musical meter is a good example of a sound structure that is temporally organized in a hierarchical manner, with recent studies showing that meter optimizes neu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72714-z
更新日期:2020-09-29 00:00:00
abstract::Miscanthus is a rhizomatous C4 grass which is considered as potential high-yielding energy crop with the low-nutrient requirements, high water-use efficiency, and capability of C mitigation. To better understand the genetic basis, an integrative analysis of the transcriptome and proteome was performed to identify impo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14151-z
更新日期:2017-10-23 00:00:00