Abstract:
:Metformin has demonstrated substantial potential for use in cancer treatments. Liver kinase B (LKB)-AMP-activated protein kinase (AMPK) and mTOR are reported to be the main targets of metformin in relation to its ability to prevent cancer cell proliferation. However, the role of metformin in the control of neoplastic cancer cell growth is possibly independent of LKB-AMPK and mTOR. Using C. elegans as a model, we found that the neuronal Q-cell divisions in L1-arrested worms were suppressed following metformin treatment in AMPK-deficient mutants, suggesting that the mechanism by which metformin suppresses these cell divisions is independent of AMPK. Our results showed that the mTOR pathway indeed played a role in controlling germ cell proliferation, but it was not involved in the neuronal Q-cell divisions occurring in L1-arrested worms. We found that the neuronal Q-cells divisions were held at G1/S cell stage by metformin in vivo. Additionally, we demonstrated that metformin could reduce the phosphorylation activity of BRAF and block the BRAF-MAPK oncogenesis pathway to regulate neuronal Q-cell divisions during L1 arrest. This work discloses a new mechanism by which metformin treatment acts to promote neuronal cancer prevention, and these results will help promote the study of the anticancer mechanisms underlying metformin treatments.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Qu Z,Ji S,Zheng Sdoi
10.3390/ijms22010178subject
Has Abstractpub_date
2020-12-26 00:00:00issue
1issn
1422-0067pii
ijms22010178journal_volume
22pub_type
杂志文章abstract::The objective of the present study is to determine whether methoxychlor (MXC) exposure in adulthood affects rat Leydig cell regeneration and to compare its effects with estradiol (E2). Adult 90-day-old male Sprague-Dawley rats received ethane dimethane sulfonate (EDS) to eliminate the adult Leydig cell population. Sub...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15057812
更新日期:2014-05-06 00:00:00
abstract::Aqueous solutions of ethoxylated alcohols which form lyotropic liquid crystals at high concentrations (40-80%) were selected as model lubricating substances. Microscopic studies under polarized light and viscosity measurements were carried out in order to confirm the presence of liquid crystalline structures in the ca...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms11010189
更新日期:2010-01-12 00:00:00
abstract::Double-spikes Phalaenopsis orchids have greater market value than those with single-spike. In this study, a gene designated as Spike Activator 1 (SPK1), which encodes a basic helix-loop-helix (bHLH) transcription factor, was isolated and characterized from Phalaenopsis aphrodite (moth orchid). SPK1 was highly expresse...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20215406
更新日期:2019-10-30 00:00:00
abstract::Chondrocyte hypertrophy is a hallmark of osteoarthritis (OA) pathology. In the present study, we elucidated the mechanism underlying the relationship between the hypertrophy/apoptotic phenotype and OA pathogenesis in bone marrow-derived mesenchymal stem cells (BM-MSCs) via gene targeting of distal-less homeobox 5 (DLX...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21144823
更新日期:2020-07-08 00:00:00
abstract::Down syndrome (DS) or trisomy of chromosome 21 (Hsa21) is characterized by impaired hippocampal-dependent learning and memory. These alterations are due to defective neurogenesis and to neuromorphological and functional anomalies of numerous neuronal populations, including hippocampal granular cells (GCs). It has been...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22031259
更新日期:2021-01-27 00:00:00
abstract::d-tagatose, a monosaccharide as well as a dietary supplement, has been reported as having a wide range of applicability in the food industry, however, the prebiotic activity, anticonstipation effects, and related mechanisms are still unclear. In this study, using the loperamide-induced constipation Kunming mice as the...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20225721
更新日期:2019-11-14 00:00:00
abstract::We have tested the hypothesis that neuropathic pain acting as a stressor drives functional plasticity in the sympathoadrenal system. The relation between neuropathic pain and adrenal medulla function was studied with behavioral, immunohistochemical and electrophysiological techniques in rats subjected to chronic const...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20010155
更新日期:2019-01-03 00:00:00
abstract::Aging is associated with a dysregulation of the immune system, leading to a general pro-inflammatory state of the organism, a process that has been named inflamm-aging. Oxidative stress has an important role in aging and in the regulation of immune responses, probably playing a role in the development of age-related d...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18122612
更新日期:2017-12-05 00:00:00
abstract::Myoblasts, the contractile cells of skeletal muscle, have been invaluable for fundamental studies of muscle development and clinical applications for muscle loss. A major limitation to the myoblast-based therapeutic approach is contamination with non-contractile fibroblasts, which overgrow during cell expansion. To ov...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18112242
更新日期:2017-10-30 00:00:00
abstract::Duloxetine (DLX) is a potent drug investigated for the treatment of depression and urinary incontinence. DLX is extensively metabolized in the liver by two P450 isozymes, CYP2D6 and CYP1A2. Propolis (PPL) is one of the popular functional foods known to have effects on activities of CYPs, including CYP1A2. Due to the h...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21051862
更新日期:2020-03-09 00:00:00
abstract::The lipophilic composition of wild Arbutus unedo L. berries, collected from six locations in Penacova (center of Portugal), as well as some general chemical parameters, namely total soluble solids, pH, titratable acidity, total phenolic content and antioxidant activity was studied in detail to better understand its po...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160614194
更新日期:2015-06-23 00:00:00
abstract::Acidic environments, such as in inflamed tissues, favor the charged form of local anesthetics (LA). Hence, these drugs show less cell permeation and diminished potency. Since the analgesic capsaicin (CAP) triggers opening of the TRPV1 receptor pore, its combination with LAs could result in better uptake and improved a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21165741
更新日期:2020-08-10 00:00:00
abstract::Liquid water is considered to be a peculiar example of glass forming materials because of the possibility of giving rise to amorphous phases with different densities and of the thermodynamic anomalies that characterize its supercooled liquid phase. In the present work, literature data on the density of bulk liquid wat...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21197269
更新日期:2020-10-01 00:00:00
abstract::In the protozoan ciliate Euplotes, a transduction pathway resulting in a mitogenic cell growth response is activated by autocrine receptor binding of cell type-specific, water-borne signaling protein pheromones. In Euplotes raikovi, a marine species of temperate waters, this transduction pathway was previously shown t...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms14047457
更新日期:2013-04-03 00:00:00
abstract:BACKGROUND:Growth failure and delayed puberty are well known features of children and adolescents with inflammatory bowel disease (IBD), in addition to the chronic course of the disease. Urinary metabonomics was applied in order to better understand metabolic changes between healthy and IBD children. METHODS:21 Pediat...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17081310
更新日期:2016-08-11 00:00:00
abstract::In eukaryotes, DNA is compacted into a complex structure known as chromatin. The unravelling of DNA is a crucial step in DNA repair, replication, transcription and recombination as this allows access to DNA for these processes. Failure to package DNA into the nucleosome, the individual unit of chromatin, can lead to g...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms12118063
更新日期:2011-01-01 00:00:00
abstract::Psychedelic and stimulating phenethylamines belong to the family of new psychoactive substances (NPS). The acute toxicity framework has begun to be investigated, while studies showing genotoxic potential are very limited or not available. Therefore, in order to fill this gap, the aim of the present work was to evaluat...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21249616
更新日期:2020-12-17 00:00:00
abstract::General cognitive (intelligence) function is substantially heritable, and is a major determinant of economic and health-related life outcomes. Cognitive impairments and intelligence decline are core features of schizophrenia which are evident before the onset of the illness. Genetic overlaps between cognitive impairme...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19123822
更新日期:2018-11-30 00:00:00
abstract::Diabetic nephropathy (DN) is the leading cause of end-stage renal disease in diabetes mellitus. Oxidative stress, insulin resistance and pro-inflammatory cytokines have been shown to play an important role in pathogeneses of renal damage on type 2 diabetes mellitus (DM). Inonotus obliquus (IO) is a white rot fungus th...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17091535
更新日期:2016-09-13 00:00:00
abstract::NASH is becoming increasingly common worldwide because of the growing global prevalence of obesity and consequently NAFLD. Unfortunately, the mechanism of progression of NAFLD to NASH and then cirrhosis is not completely understood. Several factors, including insulin resistance, inflammation, oxidative stress, lipotox...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19072034
更新日期:2018-07-13 00:00:00
abstract::Bacteria belonging to the Pectobacterium genus are the causative agents of the blackleg and soft-rot diseases that affect potato plants and tubers worldwide. In Pectobacterium, the expression of the virulence genes is controlled by quorum-sensing (QS) and N-acylhomoserine lactones (AHLs). In this work, we screened a c...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms141019976
更新日期:2013-10-08 00:00:00
abstract::Acute kidney injury (AKI) is a fatal medical episode caused by sudden kidney damage or failure, leading to the death of patients within a few hours or days. Previous studies demonstrated that exosomes derived from various mesenchymal stem/stromal cells (MSC-exosomes) have positive effects on renal injuries in multiple...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21134774
更新日期:2020-07-05 00:00:00
abstract::A bstract: Quorum sensing (QS) enables single-celled bacteria to communicate with chemical signals in order to synchronize group-level bacterial behavior. Pseudoalteromonas are marine bacteria found in versatile environments, of which QS regulation for their habitat adaptation is extremely fragmentary. To distinguish ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19113636
更新日期:2018-11-18 00:00:00
abstract::In vivo inoculation of cells such as tumor cells and induced pluripotent stem (iPS)/embryonic stem (ES) cells into immunocompromised mice has been considered as a powerful technique to evaluate their potential to proliferate or differentiate into various cell types originating from three germ cell layers. Subcutaneous...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18081678
更新日期:2017-08-02 00:00:00
abstract::The chloromethyl-functionalized polystyrene is the most commonly used ammonium cation precursor for making anion exchange resins (AER) and membranes (AEM). However, the chloromethylation of polystyrene or styrene involves highly toxic and carcinogenic raw materials (e.g., chloromethyl ether) and the resultant ammonium...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21249404
更新日期:2020-12-10 00:00:00
abstract::Triazophos is a widely used organophosphorous insecticide that has potentially adverse effects to organisms. In the present study, a high-affinity single-chain variable fragment (scFv) antibody with specific lambda light chain was developed for residue monitoring. First, the specific variable regions were correctly am...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17060823
更新日期:2016-06-07 00:00:00
abstract::Evidence suggests that high-density lipoprotein (HDL) components distinct from cholesterol, such as sphingosine-1-phosphate (S1P), may account for the anti-atherothrombotic effects attributed to this lipoprotein. The current method for the determination of plasma levels of S1P as well as levels associated with HDL par...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18081800
更新日期:2017-08-18 00:00:00
abstract::The progressive decline of the nervous system, including protein aggregate formation, reflects the subtle dysregulation of multiple functional pathways. Our previous work has shown intermittent fasting (IF) enhances longevity, maintains adult behaviors and reduces aggregates, in part, by promoting autophagic function ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19041140
更新日期:2018-04-10 00:00:00
abstract::GM2 gangliosidoses are a group of pathologies characterized by GM2 ganglioside accumulation into the lysosome due to mutations on the genes encoding for the β-hexosaminidases subunits or the GM2 activator protein. Three GM2 gangliosidoses have been described: Tay-Sachs disease, Sandhoff disease, and the AB variant. Ce...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21176213
更新日期:2020-08-27 00:00:00
abstract::Ubiquitination is a reversible post-translational modification involved in a plethora of different physiological functions. Among the substrates that are ubiquitinated, neurotrophin receptors (TrkA, TrkB, TrkC, and p75NTR) have been studied recently. TrkA is the most studied receptor in terms of its ubiquitination, an...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18030630
更新日期:2017-03-14 00:00:00