Abstract：
:Prenatal smoke exposure (PreSE) is a risk factor for nicotine dependence, which is further enhanced by postnatal smoke exposure (PostSE). One susceptibility gene to nicotine dependence is Cytochrome P450 (CYP) 2A6, an enzyme responsible for the conversion of nicotine to cotinine in the liver. Higher CYP2A6 activity is associated with nicotine dependence and could be regulated through DNA methylation. In this study we investigated whether PostSE further impaired PreSE-induced effects on nicotine metabolism, along with Cyp2a5, orthologue of CYP2A6, mRNA expression and DNA methylation. Using a mouse model where prenatally smoke-exposed adult offspring were exposed to cigarette smoke for 3 months, enzyme activity, mRNA levels, and promoter methylation of hepatic Cyp2a5 were evaluated. We found that in male offspring, PostSE increased PreSE-induced cotinine levels and Cyp2a5 mRNA expression. In addition, both PostSE and PreSE changed Cyp2a5 DNA methylation in male groups. PreSE however decreased cotinine levels whereas it had no effect on Cyp2a5 mRNA expression or methylation. These adverse outcomes of PreSE and PostSE were most prominent in males. When considered in the context of the human health aspects, the combined effect of prenatal and adolescent smoke exposure could lead to an accelerated risk for nicotine dependence later in life.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Lkhagvadorj K,Zeng Z,Meyer KF,Verweij LP,Kooistra W,Reinders-Luinge M,Dijkhuizen HW,de Graaf IAM,Plösch T,Hylkema MNdoi
10.3390/ijms22010164subject
Has Abstractpub_date
2020-12-26 00:00:00issue
1issn
1422-0067pii
ijms22010164journal_volume
22pub_type
杂志文章abstract：:There have been no major improvements in the overall survival of ovarian cancer patients in recent decades. Even though more accurate surgery and more effective treatments are available, the mortality rate remains high. Given the differences in origin and the heterogeneity of these tumors, research to elucidate the si...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18081658
更新日期：2017-07-30 00:00:00
abstract：:The uptake and transport of iron (Fe) in plants are both important for plant growth and human health. However, little is known about the mechanism of Fe transport in plants, especially for crops. In the present study, the function of yellow stripe-like 13 (YSL13) in rice was analyzed. OsYSL13 was highly expressed in l...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19113537
更新日期：2018-11-09 00:00:00
abstract：:As a major site of glucose uptake following a meal, skeletal muscle has an important role in whole-body glucose metabolism. Evidence in humans and animal models of insulin resistance and type 2 diabetes suggests that alterations in mitochondrial characteristics accompany the development of skeletal muscle insulin resi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21186948
更新日期：2020-09-22 00:00:00
abstract：:Molecular Dynamics is a method of choice for membrane simulations and the rising of coarse-grained forcefields has opened the way to longer simulations with reduced calculations times. Here, we present an elastic network, SAHBNET (Surface Accessibility Hydrogen-Bonds elastic NETwork), that will maintain the structure ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms140611510
更新日期：2013-05-30 00:00:00
abstract：:Bacteria generate membrane vesicles, which are structures known as extracellular vesicles (EVs), reported to be involved in different pathogenic mechanisms, as it has been demonstrated that EVs participate in biofilm formation, cell-to-cell communication, bacteria-host interactions, and nutrients supply. EVs deliver n...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20215307
更新日期：2019-10-25 00:00:00
abstract：:Rates of loco-regional recurrence and distant metastasis remain high among head and neck squamous cell carcinoma (HNSCC) patients, despite advancing cancer treatment modalities and therapeutic agents. One area that has generated considerable interest is the immune landscape of the tumour, heralding a wave of immune ch...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21218229
更新日期：2020-11-03 00:00:00
abstract：:While mitochondrial dysfunction is acknowledged as a major feature of aging, much less is known about the role of mitochondria in extended longevity. Livers from aged (28-month-old) and extremely aged (32-month-old) rats were analyzed for citrate synthase activity, mitochondrial transcription factor A (TFAM) amount, m...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20102601
更新日期：2019-05-27 00:00:00
abstract：:Understanding how cell organelles and compartments communicate with each other has always been an important field of knowledge widely explored by many researchers. However, despite years of investigations, one point-and perhaps the only point that many agree on-is that our knowledge about cellular-signaling pathways s...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21176173
更新日期：2020-08-26 00:00:00
abstract：:Aquaporin-5 (AQP5) is a membrane water channel widely distributed in human tissues that was found up-regulated in different tumors and considered implicated in carcinogenesis in different organs and systems. Despite its wide distribution pattern and physiological importance, AQP5 short-term regulation was not reported...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17122090
更新日期：2016-12-13 00:00:00
abstract：:Obesity and diabetes are the most prevailing health concerns worldwide and their incidence is increasing at a high rate, resulting in enormous social costs. Obesity is a complex disease commonly accompanied by insulin resistance and increases in oxidative stress and inflammatory marker expression, leading to augmented...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms17040569
更新日期：2016-04-15 00:00:00
abstract：:In this review, the latest research concerning the structure and function of the 5'-terminal region of p53 mRNA was discussed. Special attention was focused on defined structural motifs which are present in this region, as well as their conservation and plausible functional role in translation. It is known that the le...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20215382
更新日期：2019-10-29 00:00:00
abstract：:Traumatic brain injury (TBI) represents an important problem of global health. The damage related to TBI is first due to the direct injury and then to a secondary phase in which neuroinflammation plays a key role. NLRP3 inflammasome is a component of the innate immune response and different diseases, such as neurodege...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21176204
更新日期：2020-08-27 00:00:00
abstract：:The present editorial aims to summarize the 17 scientific papers that have contributed to this Special Issue focusing on different aspects of basic and translational research in colorectal cancer. ...
journal_title：International journal of molecular sciences
pub_type： 社论
doi：10.3390/ijms20123095
更新日期：2019-06-25 00:00:00
abstract：:The genus of Termitomyces purchased from the market has been identified as Termitomyces eurrhizus using the Internal Transcribed Spacer (ITS) method. An α-galactosidase from T. eurrhizus (TEG), a monomeric protein with a molecular mass of 72 kDa, was purified 146 fold by employing ion exchange chromatography and gel f...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms161226159
更新日期：2015-12-08 00:00:00
abstract：:Human induced pluripotent stem cells (iPSCs) hold enormous promise for regenerative medicine. The major safety concern is the tumorigenicity of transplanted cells derived from iPSCs. A potential solution would be to introduce a suicide gene into iPSCs as a safety switch. The herpes simplex virus type 1 thymidine kinas...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20040810
更新日期：2019-02-14 00:00:00
abstract：:Pseudomonas aeruginosa and Staphylococcus aureus are two major pathogens involved in a large variety of infections. Their co-occurrence in the same site of infection has been frequently reported and is linked to enhanced virulence and difficulty of treatment. Herein, the antimicrobial and antibiofilm activities of an ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20143604
更新日期：2019-07-23 00:00:00
abstract：:Sericin is a major constituent of silk produced by silkworms. We previously found that the instillation of sericin enhanced the proliferation of corneal epithelial cells, and acted to promote corneal wound healing in both normal and diabetic model rats. However, the mechanisms by which sericin promotes the proliferati...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19041123
更新日期：2018-04-09 00:00:00
abstract：:In view of major economic problems caused by viruses, the development of genetically resistant crops is critical for breeders but remains limited by the evolution of resistance-breaking virus mutants. During the plant breeding process, the introgression of traits from Crop Wild Relatives results in a dramatic change o...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,meta分析,评审
doi：10.3390/ijms19102856
更新日期：2018-09-20 00:00:00
abstract：:Collaterals are unique blood vessels present in the microcirculation of most tissues that, by cross-connecting a small fraction of the outer branches of adjacent arterial trees, provide alternate routes of perfusion. However, collaterals are especially susceptible to rarefaction caused by aging, other vascular risk fa...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20153608
更新日期：2019-07-24 00:00:00
abstract：:In this review we discuss the development of paramagnetic liposomes incorporating MRI contrast agents and show how these are utilized in cellular imaging in vitro. Bi-functional, bi-modal imaging paramagnetic liposome systems are also described. Next we discuss the upgrading of paramagnetic liposomes into bi-modal ima...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms11041759
更新日期：2010-04-15 00:00:00
abstract：:Many heritable genetic disorders arise from nonsense mutations, which generate premature termination codons (PTCs) in transcribed mRNA. PTCs ablate protein synthesis by prematurely terminating the translation of mutant mRNA, as well as reducing mutant mRNA quantity through targeted degradation by nonsense-mediated dec...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22010344
更新日期：2020-12-31 00:00:00
abstract：:The strong association with the Major Histocompatibility Complex (MHC) class I genes represents a shared trait for a group of autoimmune/autoinflammatory disorders having in common immunopathogenetic basis as well as clinical features. Accordingly, the main risk factors for Ankylosing Spondylitis (AS), prototype of th...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21249608
更新日期：2020-12-17 00:00:00
abstract：:The activating leukocyte immunoglobulin-like receptors (LILRAs) play an important role in innate immunity. However, most of the LILRA members have not been characterized in avian species including chickens. The present study is the first attempt at cloning, structural analysis and functional characterization of two LI...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19092710
更新日期：2018-09-11 00:00:00
abstract：:Anthocyanins are the largest class of water soluble plant pigments and a common part of the human diet. They may have many potential health benefits, including antioxidant, anti-inflammatory, anti-cancer, and cardioprotective activities. However, anthocyanin metabolism is not well understood. Studies suggest that anth...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18020446
更新日期：2017-02-18 00:00:00
abstract：:The objective of the present study was to determine the antimicrobial resistance profile of planktonic and biofilm cells of Staphylococcus aureus and coagulase-negative staphylococci (CoNS). Two hundred Staphylococcus spp. strains were studied, including 50 S. aureus and 150 CoNS strains (50 S. epidermidis, 20 S. haem...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17091423
更新日期：2016-09-01 00:00:00
abstract：:Genetic analysis of plants relies on high yields of pure DNA. For the strawberry tree (Arbutus unedo) this represents a great challenge since leaves can accumulate large amounts of polysaccharides, polyphenols and secondary metabolites, which co-purify with DNA. For this specie, standard protocols do not produce effic...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12064156
更新日期：2011-01-01 00:00:00
abstract：:Platycodin D (PD), a major saponin (platycoside) in Platycodi radix (balloon flower root), has higher pharmacological activity than the other major platycosides; however, its content in the plant root is only approximately 10% (w/w) and the productivities of PD by several enzymes are still too low for industrial appli...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20163854
更新日期：2019-08-07 00:00:00
abstract：:Cystic fibrosis is an inherited multi-organ disorder caused by mutations in the CFTR gene. Patients with this disease exhibit characteristic abnormalities in the levels of unsaturated fatty acids in blood and tissue. Recent studies have uncovered an underlying biochemical mechanism for some of these changes, namely in...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms150916083
更新日期：2014-09-11 00:00:00
abstract：:The present study was designed to probe the effects of Huperzine A (HupA) on diabetes-associated cognitive decline (DACD) using a streptozotocin (STZ)-injected rat model. Diabetic rats were treated with HupA (0.05 and 0.1 mg/kg) for seven weeks. Memory functions were evaluated by the water maze test. Nissl staining wa...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15057667
更新日期：2014-05-05 00:00:00
abstract：:Proteolipid protein 2 (PLP2), a membrane protein of the endoplasmic reticulum, is related to tumor proliferation and metastasis in some human cancers, but not in gliomas. First, we performed western-blot analysis, real-time quantitative PCR and immunohistochemical stains to detect PLP2 expression in 4 glioma cell line...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19113353
更新日期：2018-10-26 00:00:00
