Abstract:
:Errors of folate/homocysteine pathways which are critical for transferring methyl groups have been suggested to affect male fertility. We aimed to evaluate the methylation patterns of the promoter of methylenetetrahydrofolate reductase (MTHFR) gene in infertile males and to investigate the association between MTHFR promoter methylation and success of sperm retrieval. Thirty-five nonobstructive azoospermic and 46 severe oligozoospermic patients constituted the study group and were compared with 49 fertile and/or normozoospermic men. The methylation status was analysed by methylation-specific polymerase chain reaction. MTHFR promoter methylation was detected in infertile men with NOA and SO in the ratio of 48.6% and 58.7%, respectively. Methylation was also observed in 51% of controls. MTHFR promoter was methylated in 65% of men with viable spermatozoon during TESE. No association was found regarding to the profile of MTHFR promoter methylation between both NOA and SO patients and controls (p = .621). There was no relation between the methylation status of MTHFR promoter and low motility and poor morphology (p = .682 and p = .413, respectively). No association was found between MTHFR promoter methylation and presence of viable spermatozoa (p = .382). Our data indicate that the promoter methylation of MTHFR gene may not be associated with male infertility.
journal_name
Andrologiajournal_title
Andrologiaauthors
Kulac T,Hekim N,Kocamanoglu F,Beyaz C,Gunes S,Asci Rdoi
10.1111/and.13942subject
Has Abstractpub_date
2020-12-28 00:00:00pages
e13942eissn
0303-4569issn
1439-0272pub_type
杂志文章相关文献
ANDROLOGIA文献大全abstract::The reduction of sperm motility and subsequently reduced ability to undergo capacitation and acrosome reaction are considered as common causes of male infertility. The β-defensin family is a group of well-known secretory proteins with antimicrobial activity that contribute to the process of "sperm maturation" during t...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13455
更新日期:2020-02-01 00:00:00
abstract::Steroid hormone progesterone has been found to play an important role in the migration of spermatozoa through the reproductive tract, as well as to induce hyperactive motility and increase sperm velocity. The aim of this study was to examine whether progesterone could induce beneficial effects in vitrified and slow-fr...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13133
更新日期:2018-12-01 00:00:00
abstract::Analysis of seminal fluid samples from 20 ADS cases is reported for the first time. A significant decrease in the total volume of complete semen sample and sperm concentration was observed. Percentage of morphologically abnormal sperms was higher in samples from ADS cases than controls, though no linear correlation wa...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1985.tb01714.x
更新日期:1985-11-01 00:00:00
abstract::One prerequisite for fertilization is the specific binding of spermatozoa to the zona pellucida. However, the factors and mechanisms involved in this gamete contact are not well understood. Gamete recognition and binding are species-specific and are controlled by oligosaccharides of the zona and their corresponding ca...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1046/j.1439-0272.2001.00412.x
更新日期:2001-09-01 00:00:00
abstract::Prostatitis has been associated with abnormal semen parameters and may be the cause of infertility in some patients. Sperm antibodies and impaired sperm motility have been observed in the semen of patients with prostatitis. More recently, seminal oxidative stress has been detected in patients with prostatitis or acces...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:
更新日期:2003-10-01 00:00:00
abstract::The aim of this study was to investigate the relationship between sulphur dioxide (SO2 ) signalling pathway and the changes in erectile function under low androgen levels. Thirty-six healthy male Sprague Dawley (SD) rats aged eight weeks were randomly divided into androgen replacement group, castration group and sham ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13167
更新日期:2019-02-01 00:00:00
abstract::For patients with threatened fertility, preservation of it is a major concern. Although promising results have been obtained in animal models using testicular germ cell suspensions, in humans, it is crucial to first develop an efficient method of cryopreservation to be able to apply to transplantation. Thus, four reli...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2012.01290.x
更新日期:2012-12-01 00:00:00
abstract::D-chloramphenicol, at concentrations of 20 and 40 micrograms/ml, inhibited over 80% of the newly synthesized mitochondrial proteins expressed by incorporation of 3H-amino acid mixture. At concentrations of 20 and 40 micrograms ml-1, D-chloramphenicol enhanced the collective motility of washed ram spermatozoa. The coll...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1994.tb00815.x
更新日期:1994-11-01 00:00:00
abstract::Androgen insensitivity syndrome (AIS) is an X-linked disorder due to mutations of androgen receptor (AR) gene. Various AR mutations have been identified, and the characterisation of these mutations greatly facilitates our understanding of AR structure-function. In this study, we have analysed an AR missense mutation (...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2011.01219.x
更新日期:2012-05-01 00:00:00
abstract::The testis of the donkey was used as a model to study age-related changes in the period between puberty and senium. From the age of 1.5 years to the middle of sexual maturity (5 to 6 years) a number of histophysiological features, all indicative of the spermatogenetic efficiency, increase continuously. Then without a ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1997.tb00310.x
更新日期:1997-05-01 00:00:00
abstract::In mammals, pituitary control of spermatogenesis varies with age. In the rat, before puberty FSH is highly active whereas LH is not; after puberty it seems that LH alone is able to support spermatogenesis. The mode of action of hormones is discussed. In the human, in case of infertility due to hypogonadotrophic hypogo...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1976.tb02134.x
更新日期:1976-01-01 00:00:00
abstract::In order to detect abnormalities in the size of the penis, firstly, the penis length must be measured correctly. Controversies about the best technique on to be used to measure the length of the penis still persist. The hypothesis of this study is to show that penile length in children is longer than measured under no...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13864
更新日期:2020-12-01 00:00:00
abstract::Mast cells in the bilateral testicular biopsies of 30 patients with a 'mixed atrophy' of seminiferous tubules were analysed. Seven biopsies from vasectomized patients served as controls. With regard to their characteristic location within testicular tissue, two groups of mast cells could be distinguished, in both cont...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1046/j.1439-0272.1999.00287.x
更新日期:1999-07-01 00:00:00
abstract::Adult male bonnet monkeys maintained under regulated light: dark conditions exhibit a nycthemeral surge of testosterone. The present study attempts to determine the effect of administration of drugs that modulate prolactin levels like ergobromocriptine (EBC) and chlorpromazine (CPZ) on testosterone production. The inj...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1990.tb01975.x
更新日期:1990-05-01 00:00:00
abstract::Psychological stress is a known aetiology of infertility. However, the mechanisms translating it to reproductive dysfunction are not fully elucidated. Three experiments were performed on Wistar rats were designed to evaluate Sertoli cell function under stress. In Experiment I, rats were randomised into three groups: s...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13958
更新日期:2021-01-19 00:00:00
abstract::Morphologically normal spermatozoa from groups of men attending the infertility clinic with normal density and motility had subcellular elemental composition measurements from various parts of the spermatozoa compared with anatomically similar spermatozoa from semen with marked oligo- or asthenozoospermia. In the two ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1988.tb01060.x
更新日期:1988-05-01 00:00:00
abstract::Several studies have shown that high oxidative stress levels are associated with varicocele. The GST (glutathione S-transferase) family of genes is critical in the protection of cells from oxidative stress because they utilise as substrates a wide variety of products of oxidative stress. The objective of this study wa...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2010.01103.x
更新日期:2012-02-01 00:00:00
abstract::Testicular torsion is a urology urgent disease which causes testicular injury and potential sterility. In this study, we explored the protective influence of rosiglitazone on testicular ischaemia-reperfusion damage. There were 28 male Sprague Dawley rats in total, which were assigned randomly to four groups. Group A w...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12947
更新日期:2018-01-09 00:00:00
abstract::The concentration of sodium and potassium ions was measured by flame photometry in human seminal plasma and human sperm cells as well as in a sperm cell suspension infected experimentally with Ureaplasma urealyticum during incubation as long as 22 hours. The sodium concentration is 86.2 +/- 21.97 mmol/l cells in sperm...
journal_title:Andrologia
pub_type: 杂志文章
doi:
更新日期:1986-03-01 00:00:00
abstract::The new electronic spermcounter was developed to make andrological routine work easier. The apparatus is a complete microcomputer and has three main functions: The counter function allows to determine the number of spermatozoa and round cells at the same time. The morphological function allows the detailed morphologic...
journal_title:Andrologia
pub_type: 杂志文章
doi:
更新日期:1982-03-01 00:00:00
abstract::The aim of this study was to investigate possible ultrasound seminal vesicle (SV) changes in infertile patients with 'hypertrophic-congestive' (HCUF) or 'fibro-sclerotic' (FSUF) ultrasound form of male accessory gland infection (MAGI) after prolonged administration of tadalafil (TAD), a selective phosphodiesterase-5 i...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12027
更新日期:2013-12-01 00:00:00
abstract::The possible physiological role of testicular corticotropin-releasing factor (CRF) in the regulation of testicular functions was studied in neonatal rats. Two microlitres of anti-CRF-antiserum (dilution: 1:10 or 1:100) was injected intratesticularly to 5 d-old rats with two testes and to hemicastrates. Five days after...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1993.tb02711.x
更新日期:1993-07-01 00:00:00
abstract::This study was a 12-week double blind placebo-controlled, randomized, parallel trial in which active treatment with different doses of Maca Gelatinizada was compared with placebo. The study aimed to demonstrate if effect of Maca on subjective report of sexual desire was because of effect on mood or serum testosterone ...
journal_title:Andrologia
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1439-0272.2002.00519.x
更新日期:2002-12-01 00:00:00
abstract::Corpus cavernosum smooth muscle cells (CCSMCs) are important functional cells for penile erection. We evaluated the effect of transforming growth factor β1 (TGFβ1) and hepatocyte growth factor (HGF) on the viability and apoptosis of CCSMCs in vitro. CCSMCs from healthy male Sprague Dawley rats were randomly divided in...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12372
更新日期:2015-11-01 00:00:00
abstract::A critical review of the gonadotropin therapy in male infertility or hypofertility is presented. In the author's opinion, the poor and conflicting results so far obtained wih this kind of treatment are mainly due to inadequate selection of the patients admitted to the treatment itself. The author stresses some points ...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0272.1981.tb00030.x
更新日期:1981-05-01 00:00:00
abstract::In sows, the oviductal sperm-binding glycoprotein (SBG), which binds to the periacrosomal region of boar sperm, has been shown to be involved in sperm selection. In this work, we isolated porcine sperm proteins that interact with SBG. One of them is identified as a homologue of human S100A7 (psoriasin). Anti-human S10...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2011.01264.x
更新日期:2012-05-01 00:00:00
abstract::Men with hypertension often develop erectile dysfunction (ED). The present study aimed to examine the effects of sodium hydrosulphide (NaHS), a hydrogen (H2 S) donor, treatment on ED in nitric oxide synthase (NOS) inhibitor (L-NAME)-induced hypertensive rats. Forty adult Sprague-Dawley rats were divided into four grou...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13240
更新日期:2019-06-01 00:00:00
abstract::Sperm morphology has been consistently correlated with fertilisation success or failure. The clinical relevance of the percentage normal spermatozoa has been a widely discussed topic amongst infertility specialists and scientists. This study aimed to evaluate the role of sperm morphology as an indicator of additional ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12324
更新日期:2015-08-01 00:00:00
abstract::The rate of increase of sperm DNA fragmentation (rsDF) in fresh and frozen-thawed and processed sperm samples after a density gradient for sperm selection was analysed after 0, 0.5, 1.5, 4.5, 6, 24, 48 and 72 h of incubation at 37 °C, in five donors with proven fertility. The results showed that: (i) sperm DNA fragmen...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2010.01022.x
更新日期:2011-12-01 00:00:00
abstract::The aim of this study was to investigate the predictive value of the parameters that might have an effect on the success of microscopic testicular sperm extraction (micro-TESE) in infertile patients with nonobstructive azoospermia (NOA). Between 2003 and 2014, 860 patients with NOA were retrospectively analysed. The e...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13441
更新日期:2019-12-01 00:00:00