Abstract:
:Cilia play critical roles during embryonic development and adult homeostasis. Dysfunction of cilia leads to various human genetic diseases, including many caused by defects in transition zones (TZs), the "gates" of cilia. The evolutionarily conserved TZ component centrosomal protein 290 (CEP290) is the most frequently mutated human ciliopathy gene, but its roles in ciliogenesis are not completely understood. Here, we report that CEP290 plays an essential role in the initiation of TZ assembly in Drosophila. Mechanistically, the N-terminus of CEP290 directly recruits DAZ interacting zinc finger protein 1 (DZIP1), which then recruits Chibby (CBY) and Rab8 to promote early ciliary membrane formation. Complete deletion of CEP290 blocks ciliogenesis at the initiation stage of TZ assembly, which can be mimicked by DZIP1 deletion mutants. Remarkably, expression of the N-terminus of CEP290 alone restores the TZ localization of DZIP1 and subsequently ameliorates the defects in TZ assembly initiation in cep290 mutants. Our results link CEP290 to DZIP1-CBY/Rab8 module and uncover a previously uncharacterized important function of CEP290 in the coordination of early ciliary membrane formation and TZ assembly.
journal_name
PLoS Bioljournal_title
PLoS biologyauthors
Wu Z,Pang N,Zhang Y,Chen H,Peng Y,Fu J,Wei Qdoi
10.1371/journal.pbio.3001034subject
Has Abstractpub_date
2020-12-28 00:00:00pages
e3001034issue
12eissn
1544-9173issn
1545-7885pii
PBIOLOGY-D-20-00071journal_volume
18pub_type
杂志文章相关文献
PLOS BIOLOGY文献大全abstract::Genetically identical cells grown in the same culture display striking cell-to-cell heterogeneity in gene expression and other traits. A crucial challenge is to understand how much of this heterogeneity reflects the noise tolerance of a robust system and how much serves a biological function. In bacteria, stochastic g...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001325
更新日期:2012-01-01 00:00:00
abstract::Pseudomonas aeruginosa is an opportunistic pathogen that often infects open wounds or patients with cystic fibrosis. Once established, P. aeruginosa infections are notoriously difficult to eradicate. This difficulty is in part due to the ability of P. aeruginosa to tolerate antibiotic treatment at the individual-cell ...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000573
更新日期:2019-12-16 00:00:00
abstract::We have obtained precatalytic (enzyme-substrate complex) and postcatalytic (enzyme-product complex) crystal structures of an active full-length hammerhead RNA that cleaves in the crystal. Using the natural satellite tobacco ringspot virus hammerhead RNA sequence, the self-cleavage reaction was modulated by substitutin...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0060234
更新日期:2008-09-30 00:00:00
abstract::In adults, the growth of blood vessels, a process known as angiogenesis, is essential for organ growth and repair. In many disorders including cancer, angiogenesis becomes excessive. The cellular origin of new vascular endothelial cells (ECs) during blood vessel growth in angiogenic situations has remained unknown. He...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001407
更新日期:2012-01-01 00:00:00
abstract::The ability to find and consume nutrient-rich diets for successful reproduction and survival is fundamental to animal life. Among the nutrients important for all animals are polyamines, a class of pungent smelling compounds required in numerous cellular and organismic processes. Polyamine deficiency or excess has detr...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002454
更新日期:2016-05-04 00:00:00
abstract::The improper activation of the Abl tyrosine kinase results in chronic myeloid leukemia (CML). The recognition of an inactive conformation of Abl, in which a catalytically important Asp-Phe-Gly (DFG) motif is flipped by approximately 180 degrees with respect to the active conformation, underlies the specificity of the ...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0040144
更新日期:2006-05-01 00:00:00
abstract::Synaptic plasticity is widely believed to constitute a key mechanism for modifying functional properties of neuronal networks. This belief implicitly implies, however, that synapses, when not driven to change their characteristics by physiologically relevant stimuli, will maintain these characteristics over time. How ...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1000136
更新日期:2009-06-23 00:00:00
abstract::The ability of cells to accurately control gene expression levels in response to extracellular cues is limited by the inherently stochastic nature of transcriptional regulation. A change in transcription factor (TF) activity results in changes in the expression of its targets, but the way in which cell-to-cell variabi...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001528
更新日期:2013-01-01 00:00:00
abstract::The ability to identify and react to novelty within the environment is fundamental to survival. Computational models emphasize the potential role of the hippocampus in novelty detection, its unique anatomical circuitry making it ideally suited to act as a comparator between past and present experience. The hippocampus...
journal_title:PLoS biology
pub_type: 杂志文章,随机对照试验
doi:10.1371/journal.pbio.0040424
更新日期:2006-11-01 00:00:00
abstract::Do neuronal oscillations play a causal role in brain function? In a study in this issue of PLOS Biology, Helfrich and colleagues address this long-standing question by attempting to drive brain oscillations using transcranial electrical current stimulation. Remarkably, they were able to manipulate visual perception by...
journal_title:PLoS biology
pub_type: 评论,杂志文章
doi:10.1371/journal.pbio.1002032
更新日期:2014-12-30 00:00:00
abstract::Fibroblastic reticular cells (FRCs) form the cellular scaffold of lymph nodes (LNs) and establish distinct microenvironmental niches to provide key molecules that drive innate and adaptive immune responses and control immune regulatory processes. Here, we have used a graph theory-based systems biology approach to dete...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002515
更新日期:2016-07-14 00:00:00
abstract::The molecular mechanisms by which floral homeotic genes act as major developmental switches to specify the identity of floral organs are still largely unknown. Floral homeotic genes encode transcription factors of the MADS-box family, which are supposed to assemble in a combinatorial fashion into organ-specific multim...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1000090
更新日期:2009-04-21 00:00:00
abstract::The endoplasmic reticulum (ER) is the site of synthesis of secreted and membrane proteins. To exit the ER, proteins are packaged into COPII vesicles through direct interaction with the COPII coat or aided by specific cargo receptors. Despite the fundamental role of such cargo receptors in protein traffic, only a few h...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001329
更新日期:2012-01-01 00:00:00
abstract::Organisms evolve two routes to surviving infections-they can resist pathogen growth (resistance) and they can endure the pathogenesis of infection (tolerance). The sum of these two properties together defines the defensive capabilities of the host. Typically, studies of animal defenses focus on either understanding re...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0060305
更新日期:2008-12-09 00:00:00
abstract::While multiple studies have reported the accelerated evolution of brain gene expression in the human lineage, the mechanisms underlying such changes are unknown. Here, we address this issue from a developmental perspective, by analyzing mRNA and microRNA (miRNA) expression in two brain regions within macaques, chimpan...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001214
更新日期:2011-12-01 00:00:00
abstract::There is currently a demand for new highly efficient and specific drugs to treat osteoporosis, a chronic bone disease affecting millions of people worldwide. We have developed a combinatorial strategy for engineering bispecific inhibitors that simultaneously target the unique combination of c-FMS and αvβ3 integrin, wh...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.2002979
更新日期:2018-08-24 00:00:00
abstract::Over the past two decades, neuroscientists have increasingly turned their attention to the question of how the brain implements decisions between differently valued options. This emerging field, called neuroeconomics, has made quick progress in identifying a plethora of brain areas that track or are modulated by rewar...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002174
更新日期:2015-06-18 00:00:00
abstract::RNA-mediated interference (RNAi) is a method to inhibit gene function by introduction of double-stranded RNA (dsRNA). Recently, an RNAi library was constructed that consists of bacterial clones expressing dsRNA, corresponding to nearly 90% of the 19,427 predicted genes of C. elegans. Feeding of this RNAi library to th...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0000012
更新日期:2003-10-01 00:00:00
abstract::The human body is a complex organism, the gross mechanical properties of which are enabled by an interconnected musculoskeletal network controlled by the nervous system. The nature of musculoskeletal interconnection facilitates stability, voluntary movement, and robustness to injury. However, a fundamental understandi...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.2002811
更新日期:2018-01-18 00:00:00
abstract::The COVID-19 pandemic has motivated many open and collaborative analytical research projects with real-world impact. However, despite their value, such activities are generally overlooked by traditional academic metrics. Science is ultimately improved by analytical work, whether ensuring reproducible and well-document...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000913
更新日期:2020-10-16 00:00:00
abstract::The oversupply of postdoctoral scholars relative to available faculty positions has led to calls for better assessment of career outcomes. Here, we report the results of a study of postdoctoral outcomes at the University of California, San Francisco, and suggest that institutions have an obligation to determine where ...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002458
更新日期:2016-05-06 00:00:00
abstract::CD1d-mediated presentation of glycolipid antigens to T cells is capable of initiating powerful immune responses that can have a beneficial impact on many diseases. Molecular analyses have recently detailed the lipid antigen recognition strategies utilized by the invariant Vα24-Jα18 TCR rearrangements of iNKT cells, wh...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001412
更新日期:2012-01-01 00:00:00
abstract::Rodent whisker input consists of dense microvibration sequences that are often temporally integrated for perceptual discrimination. Whether primary somatosensory cortex (S1) participates in temporal integration is unknown. We trained rats to discriminate whisker impulse sequences that varied in single-impulse kinemati...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002549
更新日期:2016-08-30 00:00:00
abstract::Invariant representations of stimulus features are thought to play an important role in producing stable percepts of objects. In the present study, we assess the invariance of neural representations of tactile motion direction with respect to other stimulus properties. To this end, we record the responses evoked in in...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1000305
更新日期:2010-02-02 00:00:00
abstract::Aminoacyl-tRNA synthetases (aaRSs) are well studied for their role in binding and charging tRNAs with cognate amino acids. Recent RNA interactome studies had suggested that these enzymes can also bind polyadenylated RNAs. Here, we explored the mRNA repertoire bound by several yeast aaRSs. RNA immunoprecipitation (RIP)...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000274
更新日期:2019-05-17 00:00:00
abstract::Ever since the discovery of neural stem cells in the mammalian brain, the possibility of brain tissue regeneration has captured the minds of scientists, clinicians, and the public. Neural stem cells have been envisioned as a source of donor cells for transplantation and vectors for the delivery of gene therapy. Over t...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.2002329
更新日期:2017-05-15 00:00:00
abstract::Modification of proteins by SUMO is essential for the maintenance of genome integrity. During DNA replication, the Mms21-branch of the SUMO pathway counteracts recombination intermediates at damaged replication forks, thus facilitating sister chromatid disjunction. The Mms21 SUMO ligase docks to the arm region of the ...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002089
更新日期:2015-03-12 00:00:00
abstract::Movements overtly sample sensory information, making sensory analysis an active-sensing process. In this study, we show that visual information sampling is not just locked to the (overt) movement dynamics but to the internal (covert) dynamics of cortico-motor control. We asked human participants to perform continuous ...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000898
更新日期:2020-10-20 00:00:00
abstract::HAPLESS2 (HAP2) is a broadly conserved, gamete-expressed transmembrane protein that was shown recently to be structurally homologous to viral class II fusion proteins, which initiate fusion with host cells via insertion of fusion loops into the host membrane. However, the functional conformation of the HAP2 fusion loo...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.2006357
更新日期:2018-08-13 00:00:00
abstract::The most common cystic fibrosis (CF) causing mutation, deletion of phenylalanine 508 (ΔF508 or Phe508del), results in functional expression defect of the CF transmembrane conductance regulator (CFTR) at the apical plasma membrane (PM) of secretory epithelia, which is attributed to the degradation of the misfolded chan...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002462
更新日期:2016-05-11 00:00:00