Abstract：
:Mitochondria in neurons contribute to energy supply, the regulation of synaptic transmission, Ca2+ homeostasis, neuronal excitability, and stress adaptation. In recent years, several studies have highlighted that the neurotransmitter serotonin (5-HT) plays an important role in mitochondrial biogenesis in cortical neurons, and regulates mitochondrial activity and cellular function in cardiomyocytes. 5-HT exerts its diverse actions by binding to cell surface receptors that are classified into seven distinct families (5-HT1 to 5-HT7). Recently, it was shown that 5-HT3 and 5-HT4 receptors are located on the mitochondrial membrane and participate in the regulation of mitochondrial function. Furthermore, it was observed that activation of brain 5-HT7 receptors rescued mitochondrial dysfunction in female mice from two models of Rett syndrome, a rare neurodevelopmental disorder characterized by severe behavioral and physiological symptoms. Our Western blot analyses performed on cell-lysate and purified mitochondria isolated from neuronal cell line SH-SY5Y showed that 5-HT7 receptors are also expressed into mitochondria. Maximal binding capacity (Bmax) obtained by Scatchard analysis on purified mitochondrial membranes was 0.081 pmol/mg of 5-HT7 receptor protein. Lastly, we evaluated the effect of selective 5-HT7 receptor agonist LP-211 and antagonist (inverse agonist) SB-269970 on mitochondrial respiratory chain (MRC) cytochrome c oxidase activity on mitochondria from SH-SY5Y cells. Our findings provide the first evidence that 5-HT7 receptor is also expressed in mitochondria.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Tempio A,Niso M,Laera L,Trisolini L,Favia M,Ciranna L,Marzulli D,Petrosillo G,Pierri CL,Lacivita E,Leopoldo Mdoi
10.3390/ijms21249629subject
Has Abstractpub_date
2020-12-17 00:00:00issue
24issn
1422-0067pii
ijms21249629journal_volume
21pub_type
杂志文章abstract：:In this paper, we show a strategy to modify salphen-type Schiff base ligands with naphtol (SYML1) and pyrocathecol (2,3-dihydroxyphenyl) groups (SYML2), or a combination of both (ASYML). Each of these ligands can be used to obtain polynuclear metal complexes following two different strategies. One relies on using meta...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21103574
更新日期：2020-05-18 00:00:00
abstract：:Adiponectin is the adipokine associated with insulin sensitization, reducing liver gluconeogenesis, and increasing fatty acid oxidation and glucose uptake. Adiponectin is present in the kidneys, mainly in the arterial endothelium and smooth muscle cells, as well as in the capillary endothelium, and might be considered...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21249375
更新日期：2020-12-09 00:00:00
abstract：:The protective effect of sesamin (SES) from sesame meal on NIT-1 pancreatic β-cells damaged by streptozotocin (STZ) in vitro was investigated. The cell viability, insulin secretion, the activity of superoxide dismutase(SOD), glutathione peroxidase (GSHpx) and the content of reduced glutathione (GSH) increased signific...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms131216961
更新日期：2012-12-11 00:00:00
abstract：:It has been shown that activation of adenosine monophosphate-activated protein kinase (AMPK) suppresses proliferation of a variety of tumor cells as well as nonmalignant cells. In this study, we used post-transcriptional gene silencing with small interfering RNA (siRNA) to specifically examine the effect of AMPK on pu...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17060844
更新日期：2016-05-31 00:00:00
abstract：:The structure and function of an enzyme can be altered by nanoparticles (NPs). The interaction between enzyme and NPs is governed by the key properties of NPs, such as structure, size, surface chemistry, charge and surface shape. Recent representative studies on the NP-enzyme interactions and the regulation of enzyme ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms10104198
更新日期：2009-11-20 00:00:00
abstract：:Metformin is a biguanide drug that has been used to treat type 2 diabetes mellitus for more than 60 years. The United Kingdom Prospective Diabetic Study (UKPDS) has shown metformin to improve mortality rates in diabetes patients, and recent studies suggest metformin has additional effects in treating cancer, obesity, ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19102863
更新日期：2018-09-21 00:00:00
abstract：:Pulmonary arterial hypertension (PAH) is a rare disease caused by pulmonary vascular remodeling. Current vasodilator treatments have substantially improved patients' survival. This improved survival has led to the appearance of complications related to conditions previously underdiagnosed or even ignored, such as pulm...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21072509
更新日期：2020-04-04 00:00:00
abstract：:Mg and Mg alloys are becoming more and more of interest for several applications. In the case of biomaterial applications, a special interest exists due to the fact that a predictable degradation should be given. Various investigations were made to characterize and predict the corrosion behavior in vitro and in vivo. ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150711456
更新日期：2014-06-26 00:00:00
abstract：:As the majority of experimental studies suggest cadmium being metalloestrogen, we examined cadmium/breast cancer (BC) association by histological and tumor receptor subtype in 509 invasive BC patients and 1170 controls. Urinary cadmium was determined by atomic absorption spectrometry, and categorized using tertiles of...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20123029
更新日期：2019-06-21 00:00:00
abstract：:In wheat, flag leaf, stem, chaff and awns contribute to grain filling through photosynthesis and/or re-mobilization. Environmental and genetic effects on the relative contribution of each organ were examined by analyzing the consequences of sink-source manipulations (shading and excision) and by comparing carbon isoto...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19010056
更新日期：2017-12-25 00:00:00
abstract：:Fingertip response to trauma represents a fascinating example of tissue regeneration. Regeneration derives from proliferative mesenchymal cells (blastema) that subsequently differentiate into soft and skeletal tissues. Clinically, conservative treatment of the amputated fingertip under occlusive dressing can shift the...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18051054
更新日期：2017-05-13 00:00:00
abstract：:Obesity is a worldwide epidemic problem and correlates to varieties of acute or chronic lung diseases such as acute respiratory distress syndrome, chronic obstructive pulmonary disease, and pulmonary fibrosis. An increase of leptin, a kind of adipokine, in lean mice plasma has been found to impair immune responses and...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20051078
更新日期：2019-03-02 00:00:00
abstract：:Barley (Hordeum vulgare L.) is an important cereal crop grown for both the feed and malting industries. The allelic dwarfing gene sdw1/denso has been used throughout the world to develop commercial barley varieties. Proteomic analysis offers a new approach to identify a broad spectrum of genes that are expressed in th...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms130810410
更新日期：2012-01-01 00:00:00
abstract：:Claudin-1 (CLDN1), a tight junctional protein, is highly expressed in lung cancer cells and may contribute to chemoresistance. A drug which decreases CLDN1 expression could be a chemosensitizer for enhancing the efficacy of anticancer drugs, but there is no such drug known. We found that PMTPV, a short peptide, which ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21165909
更新日期：2020-08-17 00:00:00
abstract：:Fourier transform infrared (FTIR) microspectroscopy shows potential as a benign, objective and rapid tool to screen pluripotent and multipotent stem cells for clinical use. It offers a new experimental approach that provides a holistic measurement of macromolecular composition such that a signature representing the in...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms140917453
更新日期：2013-08-26 00:00:00
abstract：:His-tag technology was applied for biosensing purposes involving multi-redox center proteins (MRPs). An overview is presented on various surfaces ranging from flat to spherical and modified with linker molecules with nitrile-tri-acetic acid (NTA) terminal groups to bind his-tagged proteins in a strict orientation. The...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms17030330
更新日期：2016-03-03 00:00:00
abstract：:Several studies have suggested that there is a link between membrane attack complex (MAC) deposition in the retina and the progression of diabetic retinopathy (DR). Our recent investigation demonstrated that circulating IgG-laden extracellular vesicles contribute to an increase in retinal vascular permeability in DR t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21051693
更新日期：2020-03-01 00:00:00
abstract：:Acidosis commonly exists in the tissue microenvironment of various pathophysiological conditions such as tumors, inflammation, ischemia, metabolic disease, and respiratory disease. For instance, the tumor microenvironment is characterized by acidosis and hypoxia due to tumor heterogeneity, aerobic glycolysis (the "War...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18020278
更新日期：2017-01-27 00:00:00
abstract：:Avascular meniscus tears show poor intrinsic regenerative potential. Thus, lesions within this area predispose the patient to developing knee osteoarthritis. Current research focuses on regenerative approaches using growth factors or mesenchymal stem cells (MSCs) to enhance healing capacity within the avascular menisc...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20051120
更新日期：2019-03-05 00:00:00
abstract：:The progressive decline of the nervous system, including protein aggregate formation, reflects the subtle dysregulation of multiple functional pathways. Our previous work has shown intermittent fasting (IF) enhances longevity, maintains adult behaviors and reduces aggregates, in part, by promoting autophagic function ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19041140
更新日期：2018-04-10 00:00:00
abstract：:Epigenetic modifications have emerged into one of the cancer hallmarks, replacing the concept of malignant pathologies as being solely genetic-based conditions. The epigenetic landscape is responsible for normal development but also for the heterogeneity among tissues in terms of gene expression patterns. Dysregulatio...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19030670
更新日期：2018-02-27 00:00:00
abstract：:Colorectal cancer (CRC) remains a serious health problem worldwide. Approximately half of patients will develop distant metastasis after CRC resection, usually with very poor prognosis afterwards. Because patient performance after distant metastasis surgery remains very heterogeneous, ranging from death within 2 years...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21155255
更新日期：2020-07-24 00:00:00
abstract：:To develop a biodegradable polymer possessing elasticity and flexibility, we synthesized MPEG-b-(PCL-co-PLA) copolymers (PC x L y A), which display specific rates of flexibility and elasticity. We synthesize the PC ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18030671
更新日期：2017-03-21 00:00:00
abstract：:With the avalanche of biological sequences generated in the postgenomic age, molecular science is facing an unprecedented challenge, i.e., how to timely utilize the huge amount of data to benefit human beings. Stimulated by such a challenge, a rapid development has taken place in molecular science, particularly in the...
journal_title：International journal of molecular sciences
pub_type： 社论
doi：10.3390/ijms151120072
更新日期：2014-11-04 00:00:00
abstract：:Marine sponges remain representative of a unique source of renewable biological materials. The demosponges of the family Ianthellidae possess chitin-based skeletons with high biomimetic potential. These three-dimensional (3D) constructs can potentially be used in tissue engineering and regenerative medicine. In this s...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20205105
更新日期：2019-10-15 00:00:00
abstract：:There is a complex collection of neuroendocrine function during the postpartum period. Prolactin (PRL) released by suckling stimulus and its PRL receptors (PRL-R) in the central nervous system (CNS) are involved in hyporesponsiveness of the hypothalamic-pituitary-adrenal (HPA) axis in lactating mammals including roden...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18071370
更新日期：2017-06-27 00:00:00
abstract：:The reaction product of nitric oxide and superoxide, peroxynitrite, is a potent biological oxidant. The most important oxidative protein modifications described for peroxynitrite are cysteine-thiol oxidation and tyrosine nitration. We have previously demonstrated that intrinsic heme-thiolate (P450)-dependent enzymatic...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14047542
更新日期：2013-04-08 00:00:00
abstract：:Human induced pluripotent stem cells (iPSCs) hold enormous promise for regenerative medicine. The major safety concern is the tumorigenicity of transplanted cells derived from iPSCs. A potential solution would be to introduce a suicide gene into iPSCs as a safety switch. The herpes simplex virus type 1 thymidine kinas...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20040810
更新日期：2019-02-14 00:00:00
abstract：:Plutonium has potential applications in energy production in well-controlled nuclear reactors. Since nuclear power plants have great merit as environmentally friendly energy sources with a recyclable system, a recycling system for extracting Pu from spent fuels using suitable extractants has been proposed. Pu leakage ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21082791
更新日期：2020-04-17 00:00:00
abstract：:Superparamagnetic iron oxide nanoparticles (SPIONs) have attracted great attention in many biomedical fields and are used in preclinical/experimental drug delivery, hyperthermia and medical imaging. In this study, biocompatible magnetite drug carriers, stabilized by a dextran shell, were developed to carry tissue plas...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18091837
更新日期：2017-08-24 00:00:00
