Abstract:
OBJECTIVES:This in vivo study compared the antibacterial effect of a self-etch adhesive with and without the brominated monomer 12-methacryloyloxydodecyl-pyridinium bromide (MDPB) on carious dentin after selective caries removal. METHODS:10 patients showing deep primary carious lesions at two posterior teeth without pulpal symptoms were included. At visit I, carious tissue was selectively removed and carious dentin was sampled with a sterile roundbur (Komet No. 18). One cavity was restored with composite (SDR, Ceram X; DENTSPLY DeTrey) using an MDPB-containing self-etch adhesive (Clearfil Protect Bond, Kuraray Noritake; PB). The other restoration served as a control (Clearfil SE Bond II, Kuraray Noritake; SE). At visit II after 8 weeks, carious dentin was sampled again. Bacterial growth in carious dentin was differentiated using microbial cultivation. Bacterial DNA from intact cells and cell-free DNA were quantified using 16S rRNA gene-based real-time PCR and the microbial community composition was analyzed by amplicon deep-sequencing. Wilcoxon test was applied for statistical analysis. RESULTS:Both treatments showed a decrease of intact bacterial cells in carious dentin at visit II compared to visit I (PB: visit I: 1.1*106, visit II: 1.7*105 (p = 0.03); SE: visit I: 1.1*107, visit II = 2.4*105 (p = 0.002)). No statistically significant reduction of cell-free bacterial DNA was detected (PB: visit I: 6.1*105, visit II: 1.6*105 (p = 0.08); SE: visit I: 5.3*105, visit II: 2.9*105 (p = 0.10)). The decrease of intact cell-derived (p = 0.371) and cell-free DNA (p = 0.455) did not differ significantly between PB and SE. Lactobacillus was most abundant within the microbial community at both visits. Alpha-diversity was not affected by treatment and samples showed high intra- and interindividual diversity. CONCLUSION AND CLINICAL SIGNIFICANCE:Both self-etch adhesives have an antibacterial effect due to a decrease of bacterial DNA after selective caries removal. However, the results do not reveal any additional antibacterial effect by MDPB. The study is registered with the German Clinical Trials Register (DRKS00011532).
journal_name
J Dentjournal_title
Journal of dentistryauthors
Schmidt J,Krohn S,Kallies R,Schneider H,Zeller K,Ziebolz D,Berg T,Haak Rdoi
10.1016/j.jdent.2020.103555subject
Has Abstractpub_date
2020-12-17 00:00:00pages
103555eissn
0300-5712issn
1879-176Xpii
S0300-5712(20)30303-1journal_volume
105pub_type
杂志文章abstract:OBJECTIVES:This systematic review aimed to verify if there is difference in the longevity of minimally invasive techniques compared to the complete replacement for the treatment of defective direct restorations in permanent teeth. DATA:The data included randomized controlled clinical trials comparing the clinical perf...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2018.09.001
更新日期:2018-11-01 00:00:00
abstract:OBJECTIVES:Purpose of this study was to determine the influence of grey-scale reversal of digital radiographic images on the detection of proximal caries. METHODS:Five observers assessed digital bitewing radiographs (Sidexis((R)), Sirona) of 320 unrestored surfaces of extracted posterior teeth embedded in 20 models, s...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2004.08.003
更新日期:2005-01-01 00:00:00
abstract:OBJECTIVES:Some studies have been conducted to evaluate the effect of different topical fluoride regimens on the remineralization of initial carious lesions. This study was conducted to compare the effects of 3 topical fluoride treatments on the surface microhardness, fluoride uptake, and fluorescence lesion area in en...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2009.10.002
更新日期:2010-02-01 00:00:00
abstract:OBJECTIVES:Wear of dental hard tissues, e.g. dental erosion, is reported to be a growing problem. A non-destructive measurement of enamel layer thickness would provide the opportunity for both early diagnosis, and longitudinal measurement of progressive enamel loss. It was the aim of this study to investigate the poten...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/s0300-5712(99)00063-9
更新日期:2000-03-01 00:00:00
abstract:OBJECTIVES:The objective of this study was to assess the clinical performance of a direct placement gallium alloy sealed with an established dentine adhesive system. In addition, microanalysis of a few gallium restorations that failed in clinical service was performed. Clinical factors such as pulpal sensitivity, fract...
journal_title:Journal of dentistry
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0300-5712(99)00051-2
更新日期:2000-02-01 00:00:00
abstract:OBJECTIVES:To investigate repairs of direct restorations by a group of Dutch general dental practitioners (GDPs) and its consequences on longevity of restorations. METHODS:Data set was based on dental records of patients attending 11 general dental practices (24 Dentists) in the Netherlands. Patients that received Cla...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2017.06.002
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVES:The objectives of this study were two-fold: (1) to determine (by surfometry) loss of deciduous and permanent enamel and dentine following 15days' exposure to a single low pH orange drink; and (2) to determine (by surfometry) loss of deciduous and permanent enamel and dentine following exposure to the product...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/s0300-5712(99)00074-3
更新日期:2000-05-01 00:00:00
abstract::Enamel blocks bearing artificial caries are used in intra-oral appliances for cariogenicity tests. These blocks are often sterilised to prevent the possibility of cross-infection via this route. This study therefore aimed to determine the effect of sterilisation methods on the structural integrity of artificial enamel...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/s0300-5712(98)00064-5
更新日期:1999-05-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to evaluate the internal adaptation and marginal properties of ceramic laminate veneers fabricated using pressable and machinable CAD/CAM techniques. MATERIALS AND METHODS:40 ceramic laminate veneers were fabricated by either milling ceramic blocks using a CAD/CAM system (group 1 n...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2012.04.019
更新日期:2012-08-01 00:00:00
abstract:OBJECTIVES:To evaluate the effect of addition of zinc oxide and copper nanoparticles (ZnO/CuNp) into universal adhesives, on antimicrobial activity (AMA), cytotoxicity (CTX), water sorption (WS) and solubility (SO), microhardness (MH) and in vitro degree of conversion (DC), as well as resin-dentin microtensile bond str...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2019.01.012
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVES:To evaluate the effect of using a bulk-fill flowable base material on fracture strength and fracture patterns of root-filled maxillary premolars with MOD preparations restored with laminate restorations. METHODS:Fifty extracted maxillary premolars were selected for the study. Standardized MOD cavities with ...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2017.05.016
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVES:During laboratory fabrication procedures and/or clinical adjustments, pressable materials: IPS Empress and Empress 2, may be ground, polished or sandblasted. These treatments may affect their strength by introducing microscopic flaws and defects. This study investigates the effect of these procedures on the ...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2003.08.006
更新日期:2004-02-01 00:00:00
abstract:OBJECTIVES:Full-ceramic systems can be produced by different techniques (layering, heatpressing, CAD/CAM) and have various compositions with different crystalline contents that may affect the optical properties of laminate restorations. METHODS:A total of 60 specimens were prepared from e.max Press, e.max CAD, Empress...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2012.11.013
更新日期:2013-08-01 00:00:00
abstract:OBJECTIVES:To investigate differentially expressed salivary peptides in the development of early childhood caries (ECC) in 3-4 year-old children. MATERIALS AND METHODS:Eighty-two caries-free children at baseline were followed-up for 1year, during which period 15 of them had developed ECC (Group C), whilst another 15 c...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2017.04.006
更新日期:2017-06-01 00:00:00
abstract:OBJECTIVE:The main aim of this in vitro study was to evaluate the influence of Streptococcus mutans on the corrosion of titanium. METHODS:S. mutans biofilms were formed on commercially pure titanium (CP-Ti) square samples (10mm×10mm×1mm) using a culture medium enriched with sucrose. Open circuit potential (OCP) and el...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2013.03.008
更新日期:2013-06-01 00:00:00
abstract:OBJECTIVES:To determine the longevity of heat-pressed glass ceramic inlays and onlays luted with solely light-curing composite resin. METHODS:The records of patients who had received a ceramic inlay or onlay at the Heidelberg University Department of Conservative Dentistry from 1993 to 2002 were evaluated. The mean ob...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2004.10.026
更新日期:2005-05-01 00:00:00
abstract:OBJECTIVE:This study aimed to evaluate the addition of copper nanoparticles (CuNp) on the clinical performance of a universal adhesive system used as etch-and-rinse (ER) and self-etch (SE). METHODS:216 restorations were randomly placed in 36 subjects according to the following groups: ERcu = etch-and-rinse with 0.1% C...
journal_title:Journal of dentistry
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.jdent.2019.103219
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVES:A low pH environment is created by cariogenic bacteria. This study was aimed to measure pH of carious lesions intraorally using a micro-pH sensor, and assess predominant acid-producing cariogens by qPCR to differentiate caries activities. METHODS:103 dentine lesions classified as active or arrested caries b...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2011.12.013
更新日期:2012-03-01 00:00:00
abstract:OBJECTIVES:To monitor zirconia-ceramic and metal-ceramic posterior FDPs with respect to survival and technical/biological complication rates. MATERIALS AND METHODS:Fifty-eight patients received 76 3- to 5-unit posterior FDPs. The sites were randomly assigned to 40 zirconia-based (ZC) and 36 metal-based (MC) FDPs. FDPs...
journal_title:Journal of dentistry
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.jdent.2018.05.015
更新日期:2018-09-01 00:00:00
abstract:OBJECTIVES:To integrate items from two widely used oral health-related quality of life (OHRQoL) questionnaires, the General Oral Health Assessment Index (GOHAI) and the Oral Impacts on Daily Performances (OIDP), as well as culturally-specific items of the Oral Health Impact Profile (OHIP) into a four-dimensional OHRQoL...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2016.06.006
更新日期:2016-10-01 00:00:00
abstract:OBJECTIVES:The development of a micro plate assay for cytotoxicity testing of dental materials based on a bioassay using brine shrimp larvae (artemia salina) as sensitive organisms. METHODS:Brine shrimp larvae are commonly used for cytotoxicity assays in pharmacology. These larvae are sensitive to toxic substances. Th...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/s0300-5712(00)00007-5
更新日期:2000-07-01 00:00:00
abstract:OBJECTIVES:To compare the effect of bioavailable gluconate-chelated stannous fluoride (SnF2) toothpaste with control toothpastes for treatment of dentine hypersensitivity (DH) and enamel erosion. DATA AND SOURCES:A Procter & Gamble Oral Care archive of clinical studies was reviewed from 2000 to 2020. Eligible studies ...
journal_title:Journal of dentistry
pub_type: 杂志文章,评审
doi:10.1016/j.jdent.2020.103566
更新日期:2020-12-28 00:00:00
abstract:OBJECTIVE:To investigate whether bleaching prior to placement of a composite veneer had an effect on the final shade of the restoration and whether this was affected by the thickness or shade of the veneer. METHODS:Twenty bovine teeth were collected, sectioned and divided into two groups. One group was the non-bleache...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2008.04.004
更新日期:2008-07-01 00:00:00
abstract:OBJECTIVES:The bonding of resin to dentine is dependent largely on surface modifications. The purpose of the present study was to test the hypothesis that a role of the metal chlorides used as primers is to initiate polymerization of resin at the resin-dentine interface, thereby affecting bond strength. METHODS:Nine p...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/s0300-5712(97)00030-4
更新日期:1998-09-01 00:00:00
abstract:OBJECTIVES:Previously, we have reported that dental composite incorporating the new monomer methacryloyloxydodecylpyridinium bromide (MDB) showed no release of antibacterial components after being cured but still exhibited antibacterial activity against Streptococcus mutants on its surface. In this study, in order to e...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/0300-5712(95)93576-n
更新日期:1995-06-01 00:00:00
abstract:OBJECTIVES:Teeth in a patient's mouth in a dental office, or in the natural environment, represent very complex stimuli for the human color vision system. Predicting their perceived color is a daunting task at best. Colorimetry is designed mainly for the evaluation of uniform, flat, opaque, materials of fairly large si...
journal_title:Journal of dentistry
pub_type: 杂志文章,评审
doi:10.1016/j.jdent.2010.05.008
更新日期:2010-01-01 00:00:00
abstract:OBJECTIVES:This randomized, parallel group, single centre clinical trial was conducted to evaluate the safety and compare the whitening efficacy for an extended wear of an experimental 9.5% H₂O₂ high-adhesion whitening strip, relative to a marketed 10% H₂O₂ control strip. METHODS:Twenty-nine eligible adult volunteers ...
journal_title:Journal of dentistry
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.jdent.2012.11.019
更新日期:2013-08-01 00:00:00
abstract:OBJECTIVES:Dentine modifies pulpward diffusion of monomers leaching from restorative materials. Thus, remaining dentine thickness must be taken into account during in vitro cytotoxicity tests. This in vitro study was designed to determine the influence of dentine permeability on the outcome of a cytotoxicity test. MET...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/s0300-5712(98)00019-0
更新日期:1998-07-01 00:00:00
abstract:OBJECTIVES:Host modifying factors, such as genetic predisposition, may increase severity of periodontitis. Genetic polymorphisms in interleukin-4 (IL-4) genes seem to influence host response to microbial challenge. Two IL-4 polymorphisms were found in association with asthma and atopy, and later with aggressive periodo...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2003.11.004
更新日期:2004-03-01 00:00:00
abstract:OBJECTIVES:To investigate in vitro a range of differently characterised toothpastes with respect to their efficacy in an erosion/abrasion setting with special emphasis on the role of the particulate ingredients. METHODS:Human enamel samples were erosively demineralised with citric acid (2min, 6×/day; 0.5%, pH 2.5; 10 ...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2016.09.005
更新日期:2016-11-01 00:00:00