Abstract:
:A new protein detector of 2,4,6-trinitrophenol (TNP, picric acid) is concisely reported herein for the first time. In brief, the gene of a specific scFv fragment, namely 3.5, was fused separately with the gene of β-lactamase and, subsequently, expressed in the periplasmic space of Escherichia coli, affording in such a way the recombinant fusion protein 3.5-scFv-β-lactamase. This bifunctional immunoreagent tool was further convincingly demonstrated for being capable to directly detect trinitrophenol-tris(hydroxymethyl)aminomethane (TNP-Tris), a less toxic TNP derivative of choice compared to TNP itself, with competitive sensitivity (50 ± 2 fmol or 175 ± 6 pg/mL).
journal_name
Nat Prod Resjournal_title
Natural product researchauthors
Simonovic M,Mix T,Glumac M,Pejin Bdoi
10.1080/14786419.2020.1860977subject
Has Abstractpub_date
2020-12-18 00:00:00pages
1-4eissn
1478-6419issn
1478-6427pub_type
杂志文章abstract::The composition and antibacterial activity of the essential oil of the aerial flowering parts of Nepeta hormozganica Jamzad have been studied. Analysis of the oil was conducted by GC-FID and GC-MS. Thirty-two components were characterized accounting for 99.4% of the total oil. Oxygenated monoterpenes (87.5%) were foun...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2017.1295237
更新日期:2017-12-01 00:00:00
abstract::Inula graveolens (L.) Desf. is an annual aromatic herb which has various uses on alternative medicine in many region of the world. In this study, antioxidant activities of ethanol and water extracts of the plant leaves were determined by in vitro DPPH method and phenolic composition of the plant sample was determined ...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2017.1350673
更新日期:2018-06-01 00:00:00
abstract::A series of unexpected triterpenic C17-[5-methyl-1,3]-oxazoles along with targeted N-propargylamides was synthesized by an interaction of acid chlorides with propargylamine hydrochloride. We proposed that the formation of methyl oxazole passes through an alternative pathway by the participation of the terminal alkyne ...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2020.1744139
更新日期:2020-03-28 00:00:00
abstract::The chemical constituents identified in the essential oils hydrodistilled from the leaves of Amomum gagnepainii T.L.Wu, K.Larsen and Turland and Amomum repoense Pierre ex Gagnep (Zingiberaceae) of Vietnam origin are reported. The chemical analyses were performed by means of gas chromatography-flame ionisation detector...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2017.1346643
更新日期:2018-02-01 00:00:00
abstract::Phytochemical investigation on the stem bark of Shorea maxwelliana yielded five oligostilbenoids identified as α-viniferin (1), maximol A (2), vaticanol A (3), suffruticosol A (4) and vaticanol G (5). Chemotaxonomy of isolated compounds was discussed briefly. Major compounds were tested for neurotoxic and cytotoxic ac...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2012.730047
更新日期:2013-01-01 00:00:00
abstract::Alzheimer's disease (AD) is a multifactorial health problem widespread over the world. Regarding the historical importance of the alkaloids in the central nervous system pharmacology they remain as promising drug candidates against AD. Seven alkaloids from Amaryllidaceae and Fabaceae were evaluated in vivo, in vitro a...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2020.1727471
更新日期:2020-02-18 00:00:00
abstract::Nine resorcinol derivatives including two new ones, 5-[(8Z,11Z,14Z)-nonadeca-8,11,14-trienyl] resorcinol (1) and 5-[(8Z,11Z,14E)-heptadeca-8,11,14-trienyl] resorcinol (2), were isolated from the leaves of Syzygium samarangense. The new structures were elucidated by means of extensive spectroscopic techniques including...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2020.1805606
更新日期:2020-08-13 00:00:00
abstract::Four fractions were prepared from the crude extract of Caesalpinia minax Hance and the inhibitory activity of nitric oxide (NO) production release of RAW 264.7 cells stimulated by lipopolysaccharide (LPS) was evaluated. The ethyl acetate (EtOAc) fraction showed obvious inhibitory effect. Bioassay-guided fractionation ...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2017.1366480
更新日期:2018-04-01 00:00:00
abstract::Three new phenanthrenone constituents, trigoxyphins U-W (1, 7 and 9), together with eight known ones, trigoxyphin M (2), 6,9-O-dimethyltrigonostemone (3), trigonstemone (4), thrigonosomone B (5), trigonochinene E (6), actephiiol A (8), epiactephilol A (10) and neoboutomannin (11), were obtained from the methanol extra...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2015.1009066
更新日期:2015-01-01 00:00:00
abstract::This study investigated the flavonoid constituents of a traditional Chinese medical plant Ziziphora clinopodioides Lam. by using ultra-performance liquid chromatography coupled with quadruple time-of-flight mass spectrometry and screened the active components in regulating autophagy.Normal rat kidney (NRK) cells trans...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2018.1452002
更新日期:2019-09-01 00:00:00
abstract::The current study focuses on the chemical composition, and evaluation of antioxidant and antibacterial activity of the aerial parts of Centaurea resupinata subsp. dufourii. Using different chromatographic methods nine compounds 1-9 were isolated. The structural identification of isolated compounds was achieved using s...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2020.1715397
更新日期:2020-01-23 00:00:00
abstract::This article investigates the effect of microwaves on the amount of volatile compounds Achillea tenuifolia Lam with two methods, headspace single-drop microextraction and microwave-assisted headspace single-drop microextraction (MA-SDME), for the analysis of essential oil. Solvent selection, solvent volume, microwave ...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2015.1079911
更新日期:2016-01-01 00:00:00
abstract::Extracts obtained from Cyrtocarpa procera Kunth were evaluated to determine their toxicity on Artemia salina Leach. All the extracts showed a significant activity. Bioguided fractionation of these extracts led to the isolation of beta-sitosterol and the identification of 1,3-propyl-dipentadecanoate, 3-hydroxypropyl-9-...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786410500045358
更新日期:2006-01-01 00:00:00
abstract::Two new norsesquiterpenes were isolated from the extracts of the roots and rhizomes of Ligularia lapathifolia (Franch.) Hand.-Mazz. Their structures were identified as 2-acetyl-3a-methyl-5-(2-methyl-but-2-enoyloxy)-3a,4,5,6,7,7a-hexahydro-H-indene-4-carboxylic acid (1) and 2-acetyl-8a-methyl-2-(2-methyl-but-2-enoyloxy...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/1057563031000122149
更新日期:2004-04-01 00:00:00
abstract::The stem-derived essential oil of Aglaia odorata Lour. was obtained by hydrodistillation using a Clevenger-type system. Gas chromatography-mass spectrometry analysis of the oil revealed the identification of 39 compounds, representing 76.4% of the oil; germacrene D (20.3%), α-humulene (17.1%), α-himachalene (12.7%) an...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2014.924934
更新日期:2014-01-01 00:00:00
abstract::The chemical composition and antioxidant activities of the essential oil from Nandina domestica fruits were studied for the first time. Twenty-two compounds, representing 82.79% of the oil, were identified from the oil. The major compounds were 3-hexen-1-ol (12.9%), linalool (12.3%), 2-methoxy-4-vinylphenol (9.9%), ol...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2015.1057584
更新日期:2016-01-01 00:00:00
abstract::The chemical composition of the essential oil (EO) obtained from the aerial parts of Rydingia michauxii (Briq.) Scheen & V.A. Albert was analysed by gas chromatography and gas chromatography/mass spectrometry (GC/MS). Eighty components were identified in the oil with caryophyllene oxide (20.1%), trans-verbenol (10.2%)...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2017.1343321
更新日期:2018-01-01 00:00:00
abstract::Lavandin (Lavandula × intermedia Emeric ex Loiseleur) essential oils (EOs), from Abrial, Super and Grosso cultivars, cultivated and extracted in the South East of Spain, were analysed by using GC/MS to determine their composition, in both relative (peak area) and absolute (using standard curves) concentrations. Linalo...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2015.1043632
更新日期:2016-01-01 00:00:00
abstract::The aim of the present study was to evaluate the phenolic contents in capsule and root methanolic extracts of Euphorbia retusa followed by identification of phenolic on capsule extracts through high-performance liquid chromatography (HPLC- DAD) for the first time. Likewise, the antioxidant activity of both extracts wa...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2019.1582040
更新日期:2020-12-01 00:00:00
abstract::An aroma chemical emitted from Gerris paludum insularis was analyzed by means of GC and GC-MS. The main constituent was identified as iso amyl alcohol (3-methyl-1-butanol). The chemical showed a characteristic aroma of Gerris paludum insularis. ...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786410310001620655
更新日期:2004-06-01 00:00:00
abstract::The natural product lupeol 1 was isolated from aerial parts of Vernonia scorpioides with satisfactory yield, which made it viable to be used as starting material in semisynthetic approach. Ten lupeol derivatives 2-11 were prepared by classical procedures. Including, five new esters derivatives 7-11, which were obtaine...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2017.1353982
更新日期:2018-02-01 00:00:00
abstract::A novel macrolide with dioxa and double ring pentadecanone, named as phoxalone, isolated from the culture broth of a myxobacterium Sorangium cellulosum WXNXJ-C, was well investigated. The chemical structure of Phoxalone was elucidated by spectroscopic analysis including UV, IR, (1)H NMR, MALDI-TOF-MS, and LC/MS spectr...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786410701766190
更新日期:2007-12-01 00:00:00
abstract::The dichloromethane extracts of the leaves, stem bark, bark and the roots of the three species of the primitive endemic genus Hortonia, H. angustifolia, H. floribunda and H. ovalifolia, collected from nine geographical locations ranging from lower elevations (84-420 m) to higher (2000 m) showed comparable HPLC profile...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786410701722433
更新日期:2008-01-01 00:00:00
abstract::Three new compounds; epi-mukulin, (Z)-Δ(1,2) dehydroguggulsterone and Δ(6,7)dehydro-20-hydroxygugglsterone were isolated from the n-hexane-soluble fraction (HSF) of the methanol extract of guggul, the oleogum resin of Commiphora wightii together with six known compounds: diasesartemin, (+)-epi-magnolin, (+)-diayangamb...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2012.662651
更新日期:2013-01-01 00:00:00
abstract::The aim of this study was to investigate the influence of both facility location and typology of fish farm on some ecotoxicological, qualitative and reproductive variables in European sea bass Dicentrarchus labrax L. Several variables were investigated: gonado-somatic index (GSI), liver-somatic index (LSI); 7-ethoxyre...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2012.678353
更新日期:2013-04-01 00:00:00
abstract::Coumarin (2H-1-benzopyran-2-one) is a phenolic compound derived from the shikimate pathway and synthesized by various medicinal and aromatic plants as parent molecule of a large group of secondary metabolites, namely coumarins. Its main utilization is as fixative in perfumes and flavour enhancer. Given its role as phy...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2019.1660334
更新日期:2019-09-11 00:00:00
abstract::Berteroa incana is a wild herb widespread in temperate zones which was practically not studied for its biological effects. Methanolic and aqueous extracts of B. incana were assessed for the content in polyphenols and the related antioxidant and antimicrobial activities and the polysaccharide extract for the content in...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2017.1380014
更新日期:2018-12-01 00:00:00
abstract::Penicillium polonicum MCCC3A00951 is a fungus with influenza neuraminidase (NA) inhibition activity derived from a sediment of the mangrove forest of Zhangjiangkou in Fujian province, China. Chemical investigation on an ethyl acetate extract of its fermentation led to the isolation of a new compound, 7-hydroxy-3,10-de...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2020.1817015
更新日期:2020-09-14 00:00:00
abstract::A new dehydrodigallic acid derivative (1), along with 19 known compounds, including 6 phenolic derivatives, 2 steroids and 11 triterpenoids were isolated from the ethanol extract of the root of Geum urbanum. Herein, there is the first report of steroid and triterpene in Geum urbanum. The purified metabolites were char...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786419.2018.1425844
更新日期:2018-11-01 00:00:00
abstract::From the aerial parts of Citharexylum spinosum L., one new iridoid glucoside, the 7-ss-O-acetate (1) of lamiide, along with four known iridoid glucosides, lamiide (2), lamiidoside (3), duranterectoside C (4), 8-epiloganin (5) and one known lignan glucoside (+)-lyonirenisol-3a-O-ss-D-glucopyranoside (6) were isolated. ...
journal_title:Natural product research
pub_type: 杂志文章
doi:10.1080/14786410500056694
更新日期:2006-02-01 00:00:00