Silver nanoparticles-induced H2O2 triggers apoptosis-like death and is associated with dinF in Escherichia coli.

Abstract:

:Silver nanoparticles (AgNPs) are the most widely used nanomaterials as antimicrobial agents. AgNPs have been shown to inhibit the growth of and induce apoptosis-like death in Escherichia coli. However, the precise mechanism of AgNPs-induced apoptosis-like death and association with DNA damage-inducible protein F (dinF), a gene of SOS response, is unknown. Here, AgNPs-contributing depletion of intracellular glutathione levels and deactivation of glutathione peroxidase were shown. This step, indicating disruption of the antioxidant system, resulted in overall oxidative stress. Furthermore, DNA oxidation was accompanied, leading to DNA fragmentation. In addition, AgNPs appeared to induce apoptosis-like death via the SOS response. We used sodium pyruvate - an H2O2 quencher - to study the contribution of H2O2, which showed attenuation of AgNPs-induced DNA damage, SOS response, and apoptosis-like death. In dinF mutant, the strain showed a higher degree of DNA damage and apoptotic features. In conclusion, AgNPs mediate apoptosis-like cell death by H2O2-induced oxidative DNA damage. Furthermore, our result demonstrates that dinF participates in this process, which further supports that AgNPs induces SOS response. Our findings may contribute to expanding the new applications of AgNP-based nanomaterials in biomedical fields.

journal_name

Free Radic Res

journal_title

Free radical research

authors

Kim S,Lee DG

doi

10.1080/10715762.2020.1866178

subject

Has Abstract

pub_date

2021-01-06 00:00:00

pages

1-12

eissn

1071-5762

issn

1029-2470

pub_type

杂志文章
  • Molecular and biochemical investigations of inborn errors of metabolism-altered redox homeostasis in branched-chain amino acid disorders, organic acidurias, and homocystinuria.

    abstract::India, resembling other developing nations, is confronting a hastening demographic switch to non-communicable diseases. Inborn errors of metabolism (IEM) constitute a varied heterogeneous group of disorders with variable clinical appearance, primarily in the pediatric populace. Congenital deformities and genetic disor...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715762.2021.1877286

    authors: Ray SK,Mukherjee S

    更新日期:2021-01-27 00:00:00

  • Degradation of DMPO adducts from hydroxyl and 1-hydroxyethyl radicals by rat liver microsomes.

    abstract::Hydroxyl and 1-hydroxyethyl radical adducts of 5,5-dimethylpyrroline N-oxide (DMPO) were prepared by photolysis, and mechanisms for loss of their EPR signals in rat liver microsomal suspensions were evaluated. Rates of NADPH-dependent EPR signal loss were more rapid in phosphate buffer than in Tris buffer. Addition of...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715769609149069

    authors: Reinke LA,Moore DR,McCay PB

    更新日期:1996-12-01 00:00:00

  • Membrane raft redox signalosomes in endothelial cells.

    abstract::Membrane rafts (MRs) are specialized microdomains in the cell membrane with an altered lipid composition. Upon various stimulations, MRs can be clustered to aggregate or recruit NADPH oxidase sub-units and related proteins to form MR redox signalosomes in the membrane of cells like vascular endothelial cells (ECs). Mu...

    journal_title:Free radical research

    pub_type: 杂志文章,评审

    doi:10.3109/10715762.2010.485994

    authors: Zhang C,Li PL

    更新日期:2010-08-01 00:00:00

  • Natural and newly synthesized hydroxy-1-aryl-isochromans: a class of potential antioxidants and radical scavengers.

    abstract::We investigated the antioxidant and radical scavenging activity of polyphenolic isochromans. To assess the relation between structure and scavenging properties the natural occurring 1-(3'-methoxy-4'-hydroxy)phenyl-6,7-dihydroxy-isochroman (ISO-3, three OH groups) was compared with three newly synthesized derivatives t...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760500073733

    authors: Lorenz P,Zeh M,Martens-Lobenhoffer J,Schmidt H,Wolf G,Horn TF

    更新日期:2005-05-01 00:00:00

  • Chronic antioxidant therapy reduces oxidative stress in a mouse model of Alzheimer's disease.

    abstract::Oxidative modifications are a hallmark of oxidative imbalance in the brains of individuals with Alzheimer's, Parkinson's and prion diseases and their respective animal models. While the causes of oxidative stress are relatively well-documented, the effects of chronically reducing oxidative stress on cognition, patholo...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760802644694

    authors: Siedlak SL,Casadesus G,Webber KM,Pappolla MA,Atwood CS,Smith MA,Perry G

    更新日期:2009-02-01 00:00:00

  • Haem oxygenase-1 inhibits neointimal hyperplasia in rat by histone deacetylase 2.

    abstract::It has been reported that haem oxygenase-1 (Hmox1) induction attenuates neointimal thickening. Here we further investigated the potential mechanisms regulating this important pathological process. We revealed that histone deacetylase 2 (HDAC2) was induced following Hmox1 induction under 1% oxygen treatment and this in...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715762.2018.1524578

    authors: Ni J,Yang W,Shen W,Zhang R

    更新日期:2018-10-01 00:00:00

  • Spatially resolved two-photon irradiation of an intracellular singlet oxygen photosensitizer: correlating cell response to the site of localized irradiation.

    abstract::The response of HeLa cells to subcellular spatially localized two-photon irradiation of a singlet oxygen photosensitizer (protoporphyrin IX, PpIX) using a focused laser was assessed. Upon irradiation under these conditions, a localized population of PpIX excited states can be produced with meaningful intracellular spa...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715762.2013.817670

    authors: Gollmer A,Besostri F,Breitenbach T,Ogilby PR

    更新日期:2013-09-01 00:00:00

  • Validation of markers for their relevance to health and disease.

    abstract::Validation of markers requires two different steps. The first one is the analytical validation including analytical procedures, definition of standard reference, and round-tests analysis. The second one is the assessment of the biological relevance of the selected marker. This biological relevance may be in the field ...

    journal_title:Free radical research

    pub_type: 杂志文章,评审

    doi:

    authors: Antoine JM,Diplock AT

    更新日期:2000-11-01 00:00:00

  • Comparison of the phenolic composition of fruit juices by single step gradient HPLC analysis of multiple components versus multiple chromatographic runs optimised for individual families.

    abstract::After minimal sample preparation, two different HPLC methodologies, one based on a single gradient reversed-phase HPLC step, the other on multiple HPLC runs each optimised for specific components, were used to investigate the composition of flavonoids and phenolic acids in apple and tomato juices. The principal compon...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760000300551

    authors: Bremner PD,Blacklock CJ,Paganga G,Mullen W,Rice-Evans CA,Crozier A

    更新日期:2000-06-01 00:00:00

  • NAP (davunetide) protects primary hippocampus culture by modulating expression profile of antioxidant genes during limiting oxygen conditions.

    abstract::Hypoxia is a well-known threat to neuronal cells and triggers the pathophysiological syndromes in extreme environments such as high altitudes and traumatic conditions such as stroke. Among several prophylactic molecules proven suitable for ameliorating free radical damage, NAP (an octapeptide with initial amino acids:...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715762.2015.1011153

    authors: Arya A,Meena R,Sethy NK,Das M,Sharma M,Bhargava K

    更新日期:2015-04-01 00:00:00

  • Coenzyme Q10 as a possible treatment for neurodegenerative diseases.

    abstract::Coenzyme Q10 (CoQ10) is an essential cofactor of the electron transport gene as well as an important antioxidant, which is particularly effective within mitochondria. A number of prior studies have shown that it can exert efficacy in treating patients with known mitochondrial disorders. We investigated the potential u...

    journal_title:Free radical research

    pub_type: 杂志文章,评审

    doi:10.1080/10715760290021315

    authors: Beal MF

    更新日期:2002-04-01 00:00:00

  • Aldehyde adducts generated during lipid peroxidation modification of proteins.

    abstract::Various lines of evidence indicate that an important part in the pathogenesis of atherosclerosis is the modification of the plasma low-density lipoproteins (LDLs). A large number of pro-inflammatory and pro-atherogenic properties have been ascribed to the oxidatively modified LDLs and their components. There is consid...

    journal_title:Free radical research

    pub_type: 杂志文章,评审

    doi:10.3109/10715762.2015.1036052

    authors: Uchida K

    更新日期:2015-01-01 00:00:00

  • Absence of synproportionation between oxy and ferryl leghemoglobin. off.

    abstract::The synproportionation reaction between ferryl leghemoglobin and oxyleghemoglobin does not occur, at least under conditions where this process could be clearly demonstrated with myoglobin and hemoglobin. In contrast, a cross synproportionation can occur between oxyleghemoglobin and ferryl myoglobin or between ferryl l...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715769709097848

    authors: Mathieu C,Swaraj K,Davies MJ,Trinchant JC,Puppo A

    更新日期:1997-08-01 00:00:00

  • BSC2 enhances cell resistance to AmB by inhibiting oxidative damage in Saccharomyces cerevisiae.

    abstract::Amphotericin B has been the gold standard for the treatment of invasive mycosis for many years. Its resistance mechanisms are reported to be mainly related to the decrease of ergosterol content or the changes of cell wall. Previous study has shown that Saccharomyces cerevisiae strain lack of BSC2 was sensitive signifi...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715762.2020.1751151

    authors: Kong Y,Wang Q,Cao F,Zhang X,Fang Z,Shi P,Wang H,Shen Y,Huang Z

    更新日期:2020-04-01 00:00:00

  • Quantum mechanical and experimental oxidation studies of pentadecylresorcinol, olivetol, orcinol and resorcinol.

    abstract::Resorcinols (pentadecylresorcinol, olivetol, orcinol and resorcinol) exhibit antioxidant properties in liposomal systems. Antioxidant potency depends on the length of the alkyl chain. Pentadecylresorcinol has been demonstrated to be the most active antioxidant, indicating significance of its alkyl chain in a lipid bil...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715769809070804

    authors: Hładyszowski J,Zubik L,Kozubek A

    更新日期:1998-04-01 00:00:00

  • A comparison between different methods for the determination of reduced and oxidized glutathione in mammalian tissues.

    abstract::In this study, three rapid assay techniques for the determination of glutathione, one enzymatic, one fluorometric and one newly patented colorimetric method, were compared by measuring reduced (GSH) and oxidized (GSSG) glutathione in guinea-pig heart and liver. The HPLC technique was used as a standard, since it is co...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715769709084481

    authors: Floreani M,Petrone M,Debetto P,Palatini P

    更新日期:1997-05-01 00:00:00

  • The antioxidant Trolox enhances the oxidation of 2',7'-dichlorofluorescin to 2',7'-dichlorofluorescein.

    abstract::The use of antioxidants to prevent intracellular free radical damage is an area currently attracting considerable research interest. The compound 2',7'-dichlorofluorescin diacetate (DCFH-DA) is a probe for intracellular peroxide formation commonly used in such studies. During our studies we unexpectedly found that inc...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715769709097782

    authors: Kalinich JF,Ramakrishnan N,McClain DE

    更新日期:1997-01-01 00:00:00

  • The impact of different antioxidant agents alone or in combination on reactive oxygen species, antioxidant enzymes and cytokines in a series of advanced cancer patients at different sites: correlation with disease progression.

    abstract::In the present study we tested the ability of different antioxidant agents, used alone or in combination, to reduce the reactive oxygen species (ROS) levels and to increase the glutathione peroxidase (GPx) activity. Moreover, we tested the ability of such antioxidant agents to reduce the serum levels of proinflammator...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760303849

    authors: Mantovani G,Macciò A,Madeddu C,Mura L,Gramignano G,Lusso MR,Murgia V,Camboni P,Ferreli L,Mocci M,Massa E

    更新日期:2003-02-01 00:00:00

  • Oxidative stress, d-ROMs test, and ceruloplasmin.

    abstract::Human serum samples were evaluated for oxidative stress with the d-ROMs test. The ceruloplasmin (CP) and copper contents of the samples was independently measured and compared to those calculated on the basis of the d-ROMs test results for pure CP solutions. The d-ROMs readings did not show any significant correlation...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715762.2015.1136063

    authors: Colombini F,Carratelli M,Alberti A

    更新日期:2016-01-01 00:00:00

  • Fruit and vegetable and fried food consumption and 3-(2-deoxy-β-D-erythro-pentafuranosyl)pyrimido[1,2-α] purin-10(3H)-one deoxyguanosine adduct formation.

    abstract::Diet has been shown to modulate M(1)dG adduct, a biomarker of oxidative stress and lipid peroxidation. Thus, we analysed the association between diet and M(1)dG in 120 controls and 67 Map Ta Phut industrial estate workers in Rayong, Thailand, to evaluate the influence of fruit and vegetables, and fried and charcoal-gr...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715762.2011.640676

    authors: Peluso M,Munnia A,Piro S,Jedpiyawongse A,Sangrajrang S,Giese RW,Ceppi M,Boffetta P,Srivatanakul P

    更新日期:2012-01-01 00:00:00

  • Inhibition of reactive oxygen species and pre-neoplastic lesions by quercetin through an antioxidant defense mechanism.

    abstract::There is a correlation between oxidative stress generated by diethylnitrosamine (DEN) metabolism and liver cancer development. Quercetin is a flavonoid with anti-carcinogenic and antioxidant properties. This study demonstrates the mechanism of action for the chemopreventive effect of quercetin. A 10 mg/kg dose of quer...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760802626535

    authors: Vásquez-Garzón VR,Arellanes-Robledo J,García-Román R,Aparicio-Rautista DI,Villa-Treviño S

    更新日期:2009-02-01 00:00:00

  • Peroxyl radical scavenging activities of hamamelitannin in chemical and biological systems.

    abstract::The antioxidative activities of hamamelitannin (2',5-di-O-galloyl-hamamelose), gallic acid and dl-alpha-tocopherol against lipid peroxyl radicals were evaluated in chemical and biological systems. The peroxyl radical scavenging activity was evaluated by electron spin resonance (ESR) method in which both spin-trapping ...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715769509147550

    authors: Masaki H,Atsumi T,Sakurai H

    更新日期:1995-05-01 00:00:00

  • Ubiquinol supplementation protects against renal ischemia and reperfusion injury in rats.

    abstract::Generation of toxic oxygen metabolites followed by oxidant- and inflammatory-mediated tissue injury plays a crucial role in the pathogenesis of ischemia and reperfusion (IR). Ubiquinol, the reduced form of coenzyme Q10, is recognized for its potent antioxidant and anti-inflammatory properties in biological membranes. ...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715762.2013.858148

    authors: Peerapanyasut W,Thamprasert K,Wongmekiat O

    更新日期:2014-02-01 00:00:00

  • Synthesis and spin trapping properties of polystyrene supported trifluoromethylated cyclic nitrones.

    abstract::Polystyrene supported fluorinated cyclic nitrone spin-traps: Resin-2-HFDMPO (2-hydroxymethyl-2-methyl-5-(trifluoromethyl)-3,4-dihydro-2H-pyrrole-1-oxide) and Resin-2-PFDMPO (2-(3-hydroxypropyl)-2-methyl-5-(trifluoromethyl)-3,4-dihydro-2H-pyrrole 1-oxide) containing a trifluoromethyl pyrroline-N-oxide core were develop...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715762.2019.1683171

    authors: Earla A,Walter ED,Braslau R

    更新日期:2019-12-01 00:00:00

  • A validated method for rapid analysis of ethane in breath and its application in kinetic studies in human volunteers.

    abstract::Oxidative stress may initiate lipid peroxidation that generates ethane. Ethane, at low concentrations, is eliminated by pulmonary exhalation. Previous methods have not allowed frequent sampling, thus ethane kinetics has not been studied in man. A validated method over the range 3.8-100,000 ppb with a limit of quantita...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/1071576031000107353

    authors: Dale O,Bergum H,Lund T,Nilsen T,Aadahl P,Stenseth R

    更新日期:2003-08-01 00:00:00

  • Detection of a stable free radical in the B2 subunit of the manganese ribonucleotide reductase (Mn-RRase) of Corynebacterium ammoniagenes.

    abstract::Ribonucleotide reductases catalyze the irreversible reductive formation of 2'-deoxyribonucleotides required for DNA replication and cell proliferation, and a radical mechanism was assumed to be involved in this reaction. In order to search for a radical in the aerobic manganese ribonucleotide reductase (Mn-RRase) by e...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715769609088046

    authors: Griepenburg U,Lassmann G,Auling G

    更新日期:1996-06-01 00:00:00

  • Vitamin A deficiency induces prooxidant environment and inflammation in rat aorta.

    abstract::We evaluated whether nutritional vitamin A deficiency generates oxidative stress and inflammation in aorta. Wistar male rats (21 days old) were given free access to a control (8 mg retinol as retinyl palmitate/kg) or a vitamin A- deficient diet for three months. One group of deficient animals was fed with the control ...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760500072214

    authors: Gatica L,Alvarez S,Gomez N,Zago MP,Oteiza P,Oliveros L,Gimenez MS

    更新日期:2005-06-01 00:00:00

  • Local and systemic oxidant/antioxidant status before and during lung cancer radiotherapy.

    abstract::To examine local and systemic oxidative status of lung cancer (LC) and oxidant effects of radiotherapy (RT), this study evaluated antioxidants and markers of oxidative and nitrosative stress in bronchoalveolar lavage (BAL) fluid and in the blood of 36 LC patients and 36 non-cancer controls at baseline and during and a...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760902942824

    authors: Crohns M,Saarelainen S,Kankaanranta H,Moilanen E,Alho H,Kellokumpu-Lehtinen P

    更新日期:2009-07-01 00:00:00

  • Modulation of NF-κB and FOXOs by baicalein attenuates the radiation-induced inflammatory process in mouse kidney.

    abstract::The bioactive flavonoid baicalein has been shown to have radioprotective activity, although the molecular mechanism is poorly understood in vivo. C57BL/6 mice were irradiated with X-rays (15 Gy) with and without baicalein treatment (5 mg/kg/day). Irradiation groups showed an increase of NF-κB-mediated inflammatory fac...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715762.2011.555479

    authors: Lee EK,Kim JM,Choi J,Jung KJ,Kim DH,Chung SW,Ha YM,Yu BP,Chung HY

    更新日期:2011-05-01 00:00:00

  • Rapid and sensitive determination of protein-nitrotyrosine by ELISA: Application to human plasma.

    abstract::3-Nitrotyrosine (3NT) is known as an important indicator of nitrosative stress and has been linked to various diseases. Our aim was to develop an indirect ELISA (enzyme-linked immunosorbent assay) method suitable for the detection of protein-bound 3NT in clinical plasma and serum samples. Nitrated protein standards an...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715762.2011.652627

    authors: Weber D,Kneschke N,Grimm S,Bergheim I,Breusing N,Grune T

    更新日期:2012-03-01 00:00:00