当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > High-level expression and enzymatic properties of a novel thermostable xylanase with high arabinoxylan degradation ability from Chaetomium sp. suitable for beer mashing.

High-level expression and enzymatic properties of a novel thermostable xylanase with high arabinoxylan degradation ability from Chaetomium sp. suitable for beer mashing.

Abstract：

:A novel thermostable xylanase gene from Chaetomium sp. CQ31 was cloned and codon-optimized (CsXynBop). The deduced protein sequence of the gene shared the highest similarity of 75% with the glycoside hydrolase (GH) family 10 xylanase from Achaetomium sp. Xz-8. CsXynBop was over-expressed in Pichia pastoris GS115 by high-cell density fermentation, with the highest xylanase yield of 10,017 U/mL. The recombinant xylanase (CsXynBop) was purified to homogeneity and biochemically characterized. CsXynBop was optimally active at pH 6.5 and 85 °C, respectively, and stable over a broad pH range of 5.0-9.5 and up to 60 °C. The enzyme exhibited strict substrate specificity towards oat-spelt xylan (2, 489 U/mg), beechwood xylan (1522 U/mg), birchwood xylan (1067 U/mg), and showed relatively high activity towards arabinoxylan (1208 U/mg), but exhibited no activity on other tested polysaccharides. CsXynBop hydrolyzed different xylans to yield mainly xylooligosaccharides (XOSs) with degree of polymerization (DP) 2-5. The application of CsXynBop (200 U/g malt) in malt mashing substantially decreased the filtration time and viscosity of malt by 42.3% and 8.6%, respectively. These excellent characteristics of CsXynBop may make it a good candidate in beer industry.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Yu J,Liu X,Guan L,Jiang Z,Yan Q,Yang S

doi

10.1016/j.ijbiomac.2020.12.040

subject

Has Abstract

pub_date

2021-01-31 00:00:00

pages

223-232

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(20)35198-9

journal_volume

168

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES文献大全
  • A simple MWCNTs@paper biosensor for CA19-9 detection and its long-term preservation by vacuum freeze drying.

    abstract：:This paper introduces a cheap simple MWCNTs@paper biosensor for the detection of CA19-9, which is a biomarker of pancreatic cancer. By adding the CA19-9 antibody to the surface of MWCNTs which are deposited on the microporous filter paper, the correlation between the concentration of CA19-9 and resistance of biosensor...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.09.176

    authors： Jin W,Zhang R,Dong C,Jiang T,Tian Y,Yang Q,Yi W,Hou J

    更新日期：2020-02-01 00:00:00

  • Synthesis, characterization and biological evaluation of paeonol thiosemicarbazone analogues as mushroom tyrosinase inhibitors.

    abstract：:A series of hydroxy- and methoxy-substituted paeonol thiosemicarbazone analogues were synthesized as potential tyrosinase inhibitors and their inhibitory effects on mushroom tyrosinase and inhibitory mechanism were evaluated. Paeonol thiosemicarbazone analogues have been found exhibiting more remarkable inhibition tha...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijbiomac.2013.09.056

    authors： Zhu TH,Cao SW,Yu YY

    更新日期：2013-11-01 00:00:00

  • Application of two-dimensional NMR spectroscopy and molecular dynamics simulations to the conformational analysis of oligosaccharides corresponding to the cell-wall polysaccharide of Streptococcus group A.

    abstract：:This paper describes the use of a protocol for conformational analysis of oligosaccharide structures related to the cell-wall polysaccharide of Streptococcus group A. The polysaccharide features a branched structure with an L-rhamnopyranose (Rhap) backbone consisting of alternating alpha-(1-->2) and alpha-(1-->3) link...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/0141-8130(95)92678-j

    authors： Kreis UC,Varma V,Pinto BM

    更新日期：1995-06-01 00:00:00

  • Isolation, cloning and characterization of an azoreductase and the effect of salinity on its expression in a halophilic bacterium.

    abstract：:Understanding the molecular mechanisms of azo dye decolorization is important for the development of effective bioremediation for textile-colored wastewater. A halophilic bacterium Halomonas sp. strain GT was isolated, which could degrade the azo dye Acid Brilliant Scarlet GR at 10% NaCl. The complete genome sequence ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.11.175

    authors： Tian F,Guo G,Zhang C,Yang F,Hu Z,Liu C,Wang SW

    更新日期：2019-02-15 00:00:00

  • In vivo antioxidant and anti-inflammatory effects of soluble dietary fiber Konjac glucomannan in type-2 diabetic rats.

    abstract：:Although the anti-diabetic properties of Konjac glucomannan (KGM) have been reported previously; however, the molecular pathways of its anti-diabetic properties are not clear. The present study hypothesized that KGM could mitigate oxidative stress and inflammation. Three doses of KGM (40, 80, 120 mg/kg b.w.) decreased...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.05.105

    authors： Zhao Y,Jayachandran M,Xu B

    更新日期：2020-09-15 00:00:00

  • Effect of hyaluronic acid molecular weight on the morphology of quantum dot-hyaluronic acid conjugates.

    abstract：:The morphological analysis of novel quantum dot-hyaluronic acid (QDot-HA) conjugates was carried out with a transmission electron microscope (TEM). Adipic acid dihydrazide-modified HA (HA-ADH) was synthesized and conjugated to quantum dots (QDots) having carboxyl terminal ligands which were activated with 1-ethyl-3-(3...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2007.09.002

    authors： Kim J,Park K,Hahn SK

    更新日期：2008-01-01 00:00:00

  • Roles of amino acid residues H66 and D326 in the creatine kinase activity and structural stability.

    abstract：:Creatine kinase (CK) is a key enzyme for cellular energy metabolism, catalyzing the reversible phosphoryl transfer from phosphocreatine to ADP in vertebrates. CK contains a pair of highly conserved amino acids (H66 and D326) which might play an important role in sustaining the compact structure of CK by linking its N-...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.09.020

    authors： Wu QY,Wei F,Zhu YY,Tong YX,Cao J,Zhou P,Li ZY,Zeng LY,Li F,Wang XY,Xu KL

    更新日期：2018-02-01 00:00:00

  • Factors affecting the association of single- and double-stranded RNAs with montmorillonite nanoclays.

    abstract：:Montmorillonite (MMT) nanoclays exist as single and stacked sheet-like structures with large surface areas that can form stable associations with many naturally occurring biomolecules, including nucleic acids. They have been utilized successfully as vehicles for delivery of both drugs and genes into cells. Most previo...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.12.124

    authors： Gujjari A,Rodriguez BV,Pescador J,Maeder C,Beall GW,Lewis LK

    更新日期：2018-04-01 00:00:00

  • Silk sericin loaded alginate nanoparticles: Preparation and anti-inflammatory efficacy.

    abstract：:In this study, silk sericin loaded alginate nanoparticles were prepared by the emulsification method followed by internal crosslinking. The effects of various silk sericin loading concentration on particle size, shape, thermal properties, and release characteristics were investigated. The initial silk sericin loadings...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.07.018

    authors： Khampieng T,Aramwit P,Supaphol P

    更新日期：2015-09-01 00:00:00

  • pH dependent green synthesis of gold nanoparticles by completely C6-carboxylated curdlan under high temperature and various pH conditions.

    abstract：:A C6-carboxylated curdlan (C6-Cc) obtained from 4-acetamido-TEMPO-mediated oxidation of curdlan was used both as a reducing and stabilizing agent for green synthesis of pH-responsive AuNPs, which was carried out by controlling the pH of the C6-Cc solution at a high temperature (100°C). C6-Cc presented a semi-flexible ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.08.029

    authors： Qiu WY,Wang K,Wang YY,Ding ZC,Wu LX,Cai WD,Yan JK

    更新日期：2018-01-01 00:00:00

  • Effects of heat-moisture and acid treatments on the structural, physicochemical, and in vitro digestibility properties of lily starch.

    abstract：:Starch extracted from lily bulb (Lilium brownii var. Viridulum Baker) was modified via heat-moisture treatment (HMT) at different moisture levels (15-35%) and acid treatment (AT) with hydrochloric acid at five different concentrations (0.25-2.0 M). The effects of HMT and AT on the physicochemical properties and in vit...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.01.181

    authors： Li H,Wang R,Liu J,Zhang Q,Li G,Shan Y,Ding S

    更新日期：2020-04-01 00:00:00

  • ESI-MS measurements for the equilibrium constants of copper(II)-insulin complexes.

    abstract：:Trace elements regulate many biological reactions in the body. Copper(II) is known as one of trace elements and capable of binding to proteins. Insulin is a blood glucose-lowering peptide hormone and it is secreted by the pancreatic β-cells. In this study, Cu(II)-insulin complexes were investigated by using ESI-MS met...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.01.150

    authors： Gülfen M,Özdemir A,Lin JL,Chen CH

    更新日期：2018-06-01 00:00:00

  • Phosphorylated serine clusters of phosvitin plays a crucial role in the regulatory mineralization.

    abstract：:Phosphorylation of phosvitin has been proved to play an important role in the mineralization, but the active region of phosvitin is still not known yet. Four phosvitin phosphopeptides (PPPs) were obtained after separating from ion exchange column and desalting from gel filtration, and named as PPP0, PPP1, PPP3 and PPP...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.04.130

    authors： Zhang X,Huang X,Ma M

    更新日期：2018-08-01 00:00:00

  • Bioactivity and biocompatibility of a chitosan-tobermorite composite membrane for guided tissue regeneration.

    abstract：:A polymer-mineral composite membrane of the mucopolysaccharide derivative, chitosan, and calcium silicate hydrate phase, tobermorite, was prepared by solvent casting and characterised by scanning electron microscopy (SEM) and Fourier Transform infrared spectroscopy (FTIR). The bioactivity and biocompatibility of the c...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2013.11.020

    authors： Hurt AP,Getti G,Coleman NJ

    更新日期：2014-03-01 00:00:00

  • In situ decorated Au NPs on pectin-modified Fe3O4 NPs as a novel magnetic nanocomposite (Fe3O4/Pectin/Au) for catalytic reduction of nitroarenes and investigation of its anti-human lung cancer activities.

    abstract：:In recent days, the green synthesized nanomagnetic biocomposites have been evolved with tremendous potential as the future catalysts. This has encouraged us to design and synthesis of a novel Au NPs immobilized pectin modified magnetic nanoparticles (Fe3O4/Pectin/Au). It was meticulously characterized using advanced a...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.09.102

    authors： Li Y,Li N,Jiang W,Ma G,Zangeneh MM

    更新日期：2020-11-15 00:00:00

  • Developing a simultaneously antioxidant and pH-responsive κ-carrageenan/hydroxypropyl methylcellulose film blended with Prunus maackii extract.

    abstract：:к-Carrageenan and hydroxypropyl methylcellulose were used as matrix materials in which the extract of Prunus maackii pomace (EPm) was added to prepare a simultaneously antioxidant and pH-responsive film. EPm effectively increased the flexibility, barrier properties, antioxidant activity and pH-responsiveness of the fi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.11.114

    authors： Sun G,Chi W,Xu S,Wang L

    更新日期：2020-07-15 00:00:00

  • Synthesis and physico-chemical characterization of modified starches from banana (Musa AAB) and its biological activities in diabetic rats.

    abstract：:This study describes a simple method of preparation and physico-chemical properties of modified starches (type-3 resistant starches) from banana (Musa AAB), and the modified starches investigated as functional food with a beneficial effect on type-2 diabetes. RS3 was prepared using a method combined with debranching m...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.10.050

    authors： Reddy CK,Suriya M,Vidya PV,Haripriya S

    更新日期：2017-01-01 00:00:00

  • Macromolecular agents with antimicrobial potentialities: A drive to combat antimicrobial resistance.

    abstract：:In recent years, the antimicrobial resistance (AMR) or multidrug resistance (MDR) has become a serious health concern and major challenging issue, worldwide. After decades of negligence, the AMR has now captured global attention. The increasing number of antibiotic-resistant strains has threatened the achievements of ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijbiomac.2017.05.071

    authors： Bilal M,Rasheed T,Iqbal HMN,Hu H,Wang W,Zhang X

    更新日期：2017-10-01 00:00:00

  • How to calculate starch lamellar features with improved accuracy by small angle X-ray scattering.

    abstract：:Starch lamellar structures unraveled by small angle X-ray scattering (SAXS) have been critically correlated with its functionalities. However, the lamellar features calculated by the classical linear correlation function based on SAXS data are not well convincingly analyzed, because the thickness of amorphous lamellae...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.09.054

    authors： Lin L,Chi C,Wu C

    更新日期：2019-12-01 00:00:00

  • Size of the aliphatic chain of sodium houttuyfonate analogs determines their affinity for renin and angiotensin I converting enzyme.

    abstract：:Sodium houttuyfonate analogs (SHAs), CH(3)-(CH(2))(n)-CO-CH(2)-CH(OH)SO(3)Na, (n=6-14) were synthesized and their molecular interactions with renin and angiotensin I converting enzyme (ACE) studied using fluorescence quenching techniques. Unlike renin, inhibition of ACE activity was not directly proportional to the al...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2007.03.004

    authors： Yuan L,Wu J,Aluko RE

    更新日期：2007-08-01 00:00:00

  • Structure of citrus pectins and viscometric study of their solution properties.

    abstract：:Citrus pectins with degrees of methylation between 30 and 72% were carefully characterized in order to determine their charge density and molecular weight distribution, the content in galacturonic acid and in neutral sugars, the degree of methylation and acetylation. Using enzymic degradation it has been found that pe...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/0141-8130(89)90066-4

    authors： Axelos MA,Thibault JF,Lefebvre J

    更新日期：1989-06-01 00:00:00

  • Effect of nitrogen source on curdlan production by Alcaligenes faecalis ATCC 31749.

    abstract：:This study aims to investigate the effect of nitrogen source on curdlan production by Alcaligenes faecalis ATCC 31749. Curdlan production fell when excess nitrogen source was present, while biomass accumulation increased as the level of nitrogen source raised. Curdlan production and biomass accumulation were greater w...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2012.10.010

    authors： Jiang L

    更新日期：2013-01-01 00:00:00

  • Synthesis and characterization of stable and biological active chitin-based polyurethane elastomers.

    abstract：:In this work, the preparation of novel biocompatible polyurethane (PU) elastomers were carried out using curcumin and 1,4-butanediol (1,4-BDO) via step growth polymerization reaction of hydroxyl terminated polybutadiene (HTPB), toluene diisocyanate (TDI) and chitin to improve the biocompatibility, antibacterial and an...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.11.097

    authors： Amjed N,Bhatti IA,Zia KM,Iqbal J,Jamil Y

    更新日期：2020-07-01 00:00:00

  • Production of novel chia-mucilage nanocomposite films with starch nanocrystals; An inclusive biological and physicochemical perspective.

    abstract：:In the current study, chia mucilage composite films with starch nanocrystals (3% and 6%) were produced. The films were analyzed physicochemically (FT-IR, AFM, TGA, DSC), mechanically (Tensile strength and contact angle) and biologically (antimicrobial, antioxidant and cytotoxicity) properties. The incorporation of sta...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.04.146

    authors： Mujtaba M,Koc B,Salaberria AM,Ilk S,Cansaran-Duman D,Akyuz L,Cakmak YS,Kaya M,Khawar KM,Labidi J,Boufi S

    更新日期：2019-07-15 00:00:00

  • Glu residues of βDELSEED-motif are essential for peptide binding in Escherichia coli ATP synthase.

    abstract：:The current study establishes the necessity of Glu-381, Glu-384, and Glu-385 in the βDELSEED-motif of Escherichia coli ATP synthase for peptide binding and inhibition. Inhibitory profiles of wild type and mutant E. coli membrane bound F1Fo ATP synthase were studied in the presence and absence of C-terminal NH2 bound m...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.05.118

    authors： Azim S,Ahmad Z

    更新日期：2018-09-01 00:00:00

  • Transcriptome analysis of the effects of Hericium erinaceus polysaccharide on the lymphocyte homing in Muscovy duck reovirus-infected ducklings.

    abstract：:Hericium erinaceus polysaccharide (HEP) is a bioactive substance present in the fruiting bodies of H. erinaceus. Previously we have shown that HEP can repair the intestinal injury caused by Muscovy duck reovirus (MDRV) infection in Muscovy ducklings. To examine the effect of HEP on intestine mucosal MDRV immunity and ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.08.130

    authors： Liu Z,Li M,Yan P,Zhu Z,Liao L,Chen Q,Luo Y,Li H,Li J,Wang Q,Huang Y,Wu Y

    更新日期：2019-11-01 00:00:00

  • Gelatin-chitosan-pectin films incorporated with rosemary essential oil: Optimized formulation using mixture design and response surface methodology.

    abstract：:The aim of this study was to optimize the mechanical and texture properties of edible film improving its antibacterial property after adding rosemary essential oil (REO) using a Doehlert matrix. Films with the highest mechanical properties were acquired using a polymer composition of 65.2% glycerol, 24.3% gelatin, 10....

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.03.092

    authors： Yeddes W,Djebali K,Aidi Wannes W,Horchani-Naifer K,Hammami M,Younes I,Saidani Tounsi M

    更新日期：2020-07-01 00:00:00

  • Characterization of a novel oligomeric SGNH-arylesterase from Sinorhizobium meliloti 1021.

    abstract：:A novel oligomeric SGNH-arylesterase (Sm23) from Sinorhizobium meliloti 1021 was characterized using biochemical and biophysical methods. A sequence comparison of Sm23 with other SGNH members confirmed the presence of catalytic triad (Ser(10), Asp(187), and His(190)) and oxyanion holes (Ser(10)-Gly(50)-Asn(90)). The w...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2009.12.010

    authors： Hwang H,Kim S,Yoon S,Ryu Y,Lee SY,Kim TD

    更新日期：2010-03-01 00:00:00

  • Kinetics of collagen denaturation in mammalian lens capsules studied by differential scanning calorimetry.

    abstract：:Calorimetry was used to measure the rates of irreversible denaturation of collagen in intact mammalian lens capsules, isothermally stored in water at different temperatures, and the kinetic characteristics of the process determined. At 47.4 degrees C, the number of native collagen molecules declined by a factor of 10 ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/0141-8130(93)90025-h

    authors： Miles CA

    更新日期：1993-10-01 00:00:00

  • Development and in vitro characterization of chitosan/starch/halloysite nanotubes ternary nanocomposite films.

    abstract：:In this present study, ternary nanocomposite films of chitosan/starch/halloysite nanotubes (ChSHT) were fabricated by solution casting process. The ternary nanocomposite films were characterized by using various techniques such as Fourier transform infrared (FTIR), X-ray Diffraction (XRD), water contact angle, water a...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.01.047

    authors： Devi N,Dutta J

    更新日期：2019-04-15 00:00:00