Abstract:
:Juvenile hormone diol kinase (JHDK) is an important enzyme involved in the juvenile hormone metabolism pathway, which catalyzes the phosphorylation of juvenile hormone diol to form the polar metabolite JH diol phosphate. Here, we reported the first crystal structure of insect JHDK from Bombyx mori, BmJHDK-L2, determined at a resolution of 1.22 Å. The structure of BmJHDK-L2 mainly comprises of eight α-helical segments linked with loops, forming four helix-loop-helix motifs. In these four helix-loop-helix motifs with only one calcium ion bound in the first motif. Circular dichroism spectra indicated that BmJHDK-L2 has strong thermal stability, which is independent of the divalent cation. The structure of BmJHDK-L2 further allowed us to define an ATP-binding site using computational simulation and binding assays, providing a structural basis for development of inhibitor of JHDK. Moreover, the expression profile of BmJHDK-L2 indicated a predominant role in juvenile hormone metabolism in the Malpighian tubules of silkworm. Collectively, these findings expand our knowledge regarding the structural and biochemical features of insect JHDK proteins.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Xu H,Zhang Y,Zhang L,Wang Z,Guo P,Zhao Pdoi
10.1016/j.ijbiomac.2020.11.138subject
Has Abstractpub_date
2021-01-15 00:00:00pages
570-577eissn
0141-8130issn
1879-0003pii
S0141-8130(20)35065-0journal_volume
167pub_type
杂志文章abstract::The present investigation determines the extraction and characterization of seed galactomannans from the Leguminosae taxa of Bauhinia vahlii, Delonix elata, and Peltophorum pterocarpum. The seed galactomannans presented a total yield of 30.82, 24.01 and 25.25% with a Man/Gal ratios of 4.21:1, 2.55:1 and 3.03:1 for B. ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.05.080
更新日期:2019-08-01 00:00:00
abstract::A multi-functionalized poly ether sulfone (PES)/ chitosan (CS)/Fe3O4-NH2-SH nanofiber was applied for single and simultaneous removal of Pb(II) and Ni(II) ions from water systems. The structure of nanofibers was evaluated using FTIR, FESEM, BET, BJH, TGA, EDX and XPS analyses. The characterization results showed that ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.01.017
更新日期:2020-04-01 00:00:00
abstract::Coriolus versicolor has been known to be an immune stimulator effects. For further understanding of the phagocytic activity and the intracellular mechanisms of β-glucan from C. versicolor (CVG), we examined the phagocytic activity, phosphorylation of Akt and CK2, nucleus translocation of p65 and Ikaros activity in β-g...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2013.03.017
更新日期:2013-06-01 00:00:00
abstract::A novel approach in fabricating anionic inter-polymeric complex polysaccharide bio-platform with enhanced mechanical, structural and chemical properties for burn wound dressings. This approach implies a facile, insitu method to design soft nano-floral inter-polymeric complexes. In particular, sodium alginate (SA) and ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.04.157
更新日期:2020-08-15 00:00:00
abstract::The functional properties of food hydrocolloids are remarkably affected by the quality of solvent/cosolutes and temperature in a food system. In this paper, dilute solution properties of sage seed gum (SSG) as a function of salt type (NaCl, KCl, MgCl2 and CaCl2), salt concentration (10, 50, 100 and 200mM) and temperat...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2014.03.026
更新日期:2014-06-01 00:00:00
abstract::This study was conducted to confirm the effects of salinity stress on bioactive compounds and antioxidant activity of wheat microgreen extract. The microgreens were cultivated for 8 days in organic media with different concentrations of Na [0 (control), 12.5, 25, 50, and 100 mM from sodium chloride] which was containe...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.08.090
更新日期:2019-11-01 00:00:00
abstract::Boron is reported in this study to stabilize the structure of heme proteins exposed to peroxides. The oxidized heme protein (15 microM) was treated with H(2)O(2) (10mM) in 1M glycine-NaOH buffer (pH 9.2) at 25 degrees C in absence/presence of boron, and characterized by visible absorption spectroscopy, gel exclusion c...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2010.05.013
更新日期:2010-08-01 00:00:00
abstract::Electrospinning technique was used to fabricate chloroamphenicol loaded poly (L-lactic acid)/ZrO2 (CP-PLLA/ZrO2) nanofibrous membranes. The average diameter of drug carrier PLLA/ZrO2 nanofibers increased with ZrO2 concentration increasing and ribbon-shape fibers were present when ZrO2 content reached 3% and above. The...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2013.10.002
更新日期:2013-11-01 00:00:00
abstract::We isolated and purified polysaccharide from the Camellia oleifera Abel. seed cake (CCP) and studied its hypoglycemic activity in vitro. The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8. The CCP contained conjuga...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.04.054
更新日期:2018-08-01 00:00:00
abstract::In the present study, a bacterium resistance to heavy metals was isolated from contaminated areas. An eco-friendly and simple method was found to biosynthesis of silver nanoparticles (AgNPs) by reducing of aqueous Ag+ using the heavy metals resistance MKH1 bacterium. The biosynthesized AgNPs were characterized by UV-v...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.08.179
更新日期:2018-02-01 00:00:00
abstract::The effects of temperature-cycled retrogradation treatment on the structural characteristics and in vitro digestibility of waxy potato starch were investigated in this study. The results showed that the maximum yield of slowly digestible starch (SDS) in waxy potato starch reached 38.63% by retrogradation treatment und...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2014.03.007
更新日期:2014-06-01 00:00:00
abstract::Decompaction of DNA-CTA self-assembled complexes by 2-hydroxypropyl-beta-cyclodextrin (2-HP-beta-CD) was studied and the results were compared with beta-CD. Different degrees of 2-HP substitution (0.6, 0.8 and 1.0, respectively) were used and the decompaction was successful with all degrees of substitution. Fluorescen...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2009.12.002
更新日期:2010-03-01 00:00:00
abstract::Human α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase (hACMSD) is a zinc containing amidohydrolase which is a vital enzyme of the kynurenine pathway in tryptophan metabolism. It prevents the accumulation of quinolinic acid (QA) and helps in the maintenance of basal Trp-niacin ratio. To assess the structure base...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.12.005
更新日期:2018-03-01 00:00:00
abstract::Polycationic chitosan has a strong coordination to heavy metal ions due to its multifunctional hydroxyl and amino groups. However, due to the fast and facile dissolution of chitosan in acidic medium, it is difficult to measure the exact adsorption amount or coordination capacity accurately. In this work, a simple meth...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.01.203
更新日期:2020-04-01 00:00:00
abstract::к-Carrageenan and hydroxypropyl methylcellulose were used as matrix materials in which the extract of Prunus maackii pomace (EPm) was added to prepare a simultaneously antioxidant and pH-responsive film. EPm effectively increased the flexibility, barrier properties, antioxidant activity and pH-responsiveness of the fi...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.11.114
更新日期:2020-07-15 00:00:00
abstract::The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var. tuoliensis on high-fat-high-cholesterol emulsion-induced hyperlipidemic mice. The in vivo experiments demonstrated ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.11.060
更新日期:2017-02-01 00:00:00
abstract::It is of great clinical significance to design wound dressing materials with combined excellent wound healing properties and superior capability to suppress hypertrophic scar formation. This study aimed to examine if and how the cationicity of chitosan would affect the hypertrophic scar-related outcomes, through prepa...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.03.161
更新日期:2020-07-01 00:00:00
abstract::This study was designed to investigate the chemical characterization of scrophularia ningpoensis polysaccharides (SNP) and its protective activity on the cerebral ischemia/reperfusin injury (CIRI) rats. SNP was successfully isolated and fractionated by anion exchange chromatography and gel filtration into different fa...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.08.040
更新日期:2019-10-15 00:00:00
abstract::Indole-3-acetic acid (IAA)/diol based pH-sensitive biopolymeric hydrogels with tunable biological properties (cytotoxicity, anti-oxidant and anti-fungal) have been synthesized via condensation polymerization. The present study focused on the synthesis of heterocyclic hydrogel using citric acid (CA), indole-3-acetic ac...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.11.068
更新日期:2017-02-01 00:00:00
abstract::The current research work focuses on the medical application of the cost-effective cross-linked starch nanoparticles, for the transdermal delivery using Diclofenac sodium (DS) as a model drug. The prepared DS-cross-linked starch nanoparticles were synthesized using nanoprecipitation technique at different concentratio...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.09.005
更新日期:2015-11-01 00:00:00
abstract::Although synthetic antioxidant food additives are widely used in a variety of food products, some of them are suspected of having a noxious effect on human health. As a consequence, much research attention has been focused on developing natural antioxidant compounds from plants. Riang (Parkia timoriana (DC.) Merr.) is...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.09.079
更新日期:2020-12-01 00:00:00
abstract::Grifola frondosa is an edible and medicinal mushroom with great nutritional values and bioactivities. In the present study, a soluble homogeneous β-glucan, GFPS, with high molecular mass of 5.42 × 106 Da was purified from the fruit bodies of Grifola frondosa using 5% cold NaOH. The structure of GFPS was determined wit...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.01.236
更新日期:2020-04-15 00:00:00
abstract::Nanoparticles are produced by means of polyelectrolyte complexation (PEC) of oppositely charged polycationic chitosan (CH) with polyanionic polysaccharide extracted from Sterculia striata exudates (rhamnogalacturonoglycan (RG)-type polysaccharide). The nanoparticles formed with low-molar-mass CH are larger than those ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.03.070
更新日期:2016-07-01 00:00:00
abstract::Cross-linking agents play an important part in the physical properties of collagen based biomaterials. Oxazolidines are novel aldehydic tanning agents that are widely used to stabilise collagens in the leather industry. The exact mechanism through which they cross-link collagens is, however, not well understood. When ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2006.09.003
更新日期:2007-03-10 00:00:00
abstract::Polysaccharides are essential compounds that contribute to the biological activities of mushrooms. Two new galactomannans (Galactomannan I and II) were isolated from R. luteolus and G. adspersum. Their structures were characterized using FT-IR, 1D, and 2D-NMR techniques. Both isolated galactomannans I and II mainly in...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.10.040
更新日期:2020-12-15 00:00:00
abstract::Arginine kinase (AK), a crucial enzyme in energy metabolism, buffers cellular ATP levels by catalyzing the reversible phosphoryl transfer between ATP and arginine. To better understand the role of Cys271 in conformational changes of AK from greasyback shrimp (Metapenaeus ensis), we replaced the residue with serine and...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2011.04.002
更新日期:2011-07-01 00:00:00
abstract::The swollen culms of Zizania latifolia have been used as a vegetable and traditional herbal medicine in China, Japan, Korea and Southeast Asia countries. Up to date, there is little information about the polysaccharides from the swollen culms of Zizania latifolia and their potential bioactivities. In the present study...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.12.010
更新日期:2017-02-01 00:00:00
abstract::Chitosan and heparin were covalently immobilized onto a poly(lactic acid-co-glycolic acid) (PLGA) surface using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC), N-hydroxysuccinimide (NHS) in a 2-morpholinoethane sulfonic acid (MES) buffer system. The properties of the modified PLGA surface and the control were in...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/s0141-8130(03)00072-2
更新日期:2003-11-01 00:00:00
abstract::As iron supplement, the antioxidant activities of APS-iron (III) complex were comprehensively evaluated by 5-axe cobweb charts, which indicated the APS-iron (III) complex had a certain antioxidant activity and been weaker than that of APS. The results of immunological activity experiments indicated the stimulation ind...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.03.196
更新日期:2019-07-01 00:00:00
abstract::A set of fungal polysaccharide samples was characterised by elemental analysis and FTIR spectroscopy and compared with reference chitins, chitosans and β-D-glucans. The nitrogen to carbon (N/C) values and FTIR spectra were used to compare the samples based on their composition. It was found that the N/C ratio correlat...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.05.059
更新日期:2015-09-01 00:00:00