Abstract:
:Evaluation of the effect of different parameters for designing a non-viral vector in gene delivery systems has great importance. In this manner, 2D crystals, precisely layered double hydroxides, have attracted the attention of scientists due to their significant adjustability and low-toxicity and low-cost preparation procedure. In this work, the relationship between different physicochemical properties of LDH, including pH, size, zeta potential, and synthesis procedure, was investigated and optimized for CRISPR/Cas9 delivery and reverse fluorescence response to the EGFP. In this manner, ZnAl LDH and ZnAl HMTA LDH were synthesized and characterized and applied in the HEK-293 cell line to deliver CRISPR/Cas9. The results were optimized by different characterizations as well as Gel Electrophoresis and showed acceptable binding ability with the DNA that could be considered as a promising and also new gold-standard for the delivery of CRISPR/Cas9. Also, the relationship of the presence of tertiary amines (in this case, hexamethylenetetramine (HMTA) as the templates) in the structure of the ZnAl LDH, as well as the gene delivery application, was evaluated. The results showed more than 79% of relative cell viability in most of the weight ratios of LDH to CRISPR/Cas9; fully quenching the fluorescence intensity of the EGFP/LDH in the presence of 15 µg mL-1 of the protoporphyrins along with the detection limit of below 2.1 µg mL-1, the transfection efficiency of around 33% of the GFP positive cell for ZnAl LDH and more than 38% for the ZnAl LDH in the presence of its tertiary amine template.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Rabiee N,Bagherzadeh M,Ghadiri AM,Salehi G,Fatahi Y,Dinarvand Rdoi
10.1038/s41598-020-77809-1subject
Has Abstractpub_date
2020-11-26 00:00:00pages
20672issue
1issn
2045-2322pii
10.1038/s41598-020-77809-1journal_volume
10pub_type
杂志文章abstract::Glaucoma optic neuropathy (GON) is a condition where pathogenic intraocular pressure (IOP) results in axonal damage following retinal ganglion cell (RGC) death, and further results in secondary damage of the lateral geniculate nucleus (LGN). Therapeutic targets for glaucoma thus focus on both the LGN and RGC. However,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06501
更新日期:2014-10-13 00:00:00
abstract::The tumour suppressor p53 is mutated in cancer, including over 96% of high-grade serous ovarian cancer (HGSOC). Mutations cause loss of wild-type p53 function due to either gain of abnormal function of mutant p53 (mutp53), or absent to low mutp53. Massively parallel sequencing (MPS) enables increased accuracy of detec...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26191
更新日期:2016-05-18 00:00:00
abstract::The objective of this study was to examine the relationship between sleep duration and ischemic and hemorrhagic stroke in a community-based cohort. The current analysis included 95,023 Chinese participants who were free of stroke at the baseline survey (2006-2007). Cox proportional hazards models were used to calculat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33664
更新日期:2016-09-16 00:00:00
abstract::One of the ways dredging can affect benthic habitats is through high levels of sediment deposition, which has the potential to smother sessile organisms such as sponges. In order to provide pressure-response values to sedimentation and tease apart the different cause-effect pathways of high turbidity, 5 sponge species...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05243-x
更新日期:2017-07-14 00:00:00
abstract::Herein, we have fabricated rutile TiO2 nanorod-coupled α-Fe2O3 by a wet-chemical process. It is demonstrated that the visible activities for photoelectrochemical water oxidation and for degrading pollutant of α-Fe2O3 are greatly enhanced after coupling a proper amount of rutile nanorods. The enhanced activity is attri...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06180
更新日期:2014-08-26 00:00:00
abstract::Diapause, a programmed developmental arrest, is common in insects, enabling them to survive adverse seasons. It is well established that pupal diapause is regulated by ecdysteroids secreted by the prothoracic glands (PGs), with cessation of ecdysteroid secretion after pupal ecdysis leading to pupal diapause. A major f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41651
更新日期:2017-01-31 00:00:00
abstract::Current studies addressing the influence of interleukin-33 or its receptor (IL-33R/ST2) on development of atopic dermatitis-like inflammation in mice have reported conflicting results. We compared the response in single- and double-deficient IL-33-/-/ST2-/- C57BL/6J BomTac mice in the well-established calcipotriol-ind...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-63410-z
更新日期:2020-04-15 00:00:00
abstract::Emerging evidence suggests substantial health benefits from using meditation. While there are some indications that the popularity of meditation is increasing, little is known about the prevalence, patterns, and predictors of meditation use in the general population. In this secondary analysis of data from the 2012 US...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36760
更新日期:2016-11-10 00:00:00
abstract::The quantum mechanical motion of electrons and nuclei in systems spatially confined to the molecular dimensions occurs on the sub-femtosecond to the femtosecond timescales respectively. Consequently, the study of ultrafast electronic and, in specific cases, nuclear dynamics requires the availability of light pulses wi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60331-9
更新日期:2020-02-28 00:00:00
abstract::Lung cancer represents a major cause of cancer-related death worldwide. Although various tactics and anti-tumor drugs have been used to improve curative effects, five-year survival rate of lung cancer patients remains poor. In this study, we investigated the action and underlying mechanisms of our recently optimized C...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05146-x
更新日期:2017-07-10 00:00:00
abstract::Although collateral vessel growth is distinctly enhanced by elevated fluid shear stress (FSS), the underlying regulatory mechanism of this process remains incompletely understood. Recent studies have shown that microRNAs (miRNAs) play a pivotal role in vascular development, homeostasis and a variety of diseases. There...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06910-9
更新日期:2017-07-27 00:00:00
abstract::When complementary resources are required for an optimal life cycle, most animals need to move between different habitats. However, the level of connectivity between resources can vary and, hence, influence individuals' behaviour. We show that landscape composition and configuration affect the connectivity between bre...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21321-0
更新日期:2018-02-14 00:00:00
abstract::Fluorescence-activated cell sorting (FACS) applying flow cytometry to separate cells on a molecular basis is a widespread method. We demonstrate that both fluorescent and unlabeled live cells in a Petri dish observed with a microscope can be automatically recognized by computer vision and picked up by a computer-contr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01088
更新日期:2013-01-01 00:00:00
abstract::Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non-transgenic tomato variety resistant to the powdery mildew fungal pathogen using the CRISPR/Cas9 technology. We used whole-genome sequencing to show...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00578-x
更新日期:2017-03-28 00:00:00
abstract::The development of well-organized structures with high luminescent properties in the solid and aggregated states is of both scientific and technological interest due to their applications in nanotechnology. In this paper we described the synthesis of amphiphilic and dumbbell shaped AIE-active tetraphenylethylene (TPE)...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42898
更新日期:2017-02-23 00:00:00
abstract::Stroke incidence is a multifactorial disease and especially hyperhomocysteinemia is associated with a higher risk of stroke. Previous studies have reported a folate metabolism disorder associated with the MTHFR gene. We investigated four single nucleotide polymorphisms in the MTHFR 3'-UTR [2572 C > A (rs4846049), 4869...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12668-x
更新日期:2017-09-29 00:00:00
abstract::In this paper, phosphorene nanoribbons (PNRs) are theoretically studied using a multiscale simulation flow from the ab initio level to the tight binding (TB) level. The scaling effects of both armchair PNRs (aPNRs) and zigzag PNRs (zPNRs) from material properties to device properties are explored. The much larger effe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38009
更新日期:2016-11-29 00:00:00
abstract::Human protein tyrosine phosphatase non-receptor type 4 (PTPN4) has been shown to prevent cell death. The active form of human PTPN4 consists of two globular domains, a PDZ (PSD-95/Dlg/ZO-1) domain and a phosphatase domain, tethered by a flexible linker. Targeting its PDZ domain abrogates this protection and triggers a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08193-6
更新日期:2017-08-11 00:00:00
abstract::The development of effective central nervous system (CNS) drugs has been hampered by the lack of robust strategies to mimic the blood-brain barrier (BBB) and cerebrovascular impairments in vitro. Recent technological advancements in BBB modeling using induced pluripotent stem cells (iPSCs) allowed to overcome some of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52213-6
更新日期:2019-11-05 00:00:00
abstract::Controlling light and heat via metamaterials has presented interesting technological applications using transformation optics (TO) and transformation thermodynamics (TT). However, such devices are commonly mono-physics and mono-purpose, because the used metamaterial is designed to deal with one type of physical mechan...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29866-w
更新日期:2018-07-30 00:00:00
abstract::The aim of this study was to assess the systemic and analgesic effects of epidural dexmedetomidine in thoracic epidural anaesthesia (TEA) combined with total intravenous anaesthesia during thoracic surgery. Seventy-one patients undergoing open thoracotomy were included in this study and randomly divided into three gro...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/s41598-017-04382-5
更新日期:2017-06-21 00:00:00
abstract::Blood coagulation is regulated through protein-protein and protein-lipid interactions that occur at the sub-endothelium following vascular damage. Soluble clotting proteins bind to membrane components in a phosphatidylserine (PS) dependent manner to assemble multi-protein complexes that regulate clot formation; howeve...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73647-3
更新日期:2020-10-15 00:00:00
abstract::Real-time PCR targeting lytA (the major autolysin gene) and piaB (permease gene of the pia ABC transporter) are currently used as the gold-standard culture-independent assays for Streptococcus pneumoniae identification. We evaluated the performance of a new real-time PCR assay - targeting SP2020 (putative transcriptio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39791-1
更新日期:2019-03-01 00:00:00
abstract::Prostate cancer (PCa) is the most common malignancy and second leading cause of cancer-related deaths in American men. Proliferating cells have higher need for nutrients and oxygen, triggering angiogenesis that plays a critical role in tumor growth, progression and metastasis. Consequently, immense focus has converged...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27831-1
更新日期:2018-06-22 00:00:00
abstract::Clinical trials have recently demonstrated the effectiveness of Pre-Exposure Prophylaxis (PrEP) in preventing HIV infection. Consequently, PrEP may soon be used for epidemic control. We model the dynamic interactions that will occur between treatment programs and potential PrEP interventions in resource-constrained co...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00185
更新日期:2011-01-01 00:00:00
abstract::The 1111-type iron-based superconductor LnFeAsO(1-x)Fx (Ln stands for lanthanide) is the first material with a Tc above 50 K, other than cuprate superconductors. Electron doping into LaFeAsO by H, rather than F, revealed a double-dome-shaped Tc-x diagram, with a first dome (SC1, 0.05 journal_title:Scientific reports pub_type: 杂志文章 doi:10.1038/srep07829 更新日期:2015-01-16 00:00:00
abstract::The objective of this study was to investigate associations between education in early life and cognitive impairment in later life in Colombia. Participants were community-dwelling adults aged 60 years or older from the National Study of Health, Wellbeing and Ageing. Trained interviewers administered a shorter version...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74822-2
更新日期:2020-10-19 00:00:00
abstract::The threshold size for enlarged abdominal lymph nodes (E-ALNs), a common pediatric disorder, has yet to be standardized. According to the maximum short-axis diameter, this study divided ALNs into Grade A (≥ 10 mm), Grade B (8-10 mm), Grade C (5-8 mm), and Grade D (< 5 mm, normal). To identify the threshold size for E-...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-81339-9
更新日期:2021-01-19 00:00:00
abstract::Based on a panel of 30 provinces and a timeframe from January 2009 to December 2013, we estimate the association between monthly human immunodeficiency virus/acquired immune deficiency syndrome (HIV/AIDS) incidence and the relevant Internet search query volumes in Baidu, the most widely used search engine among the Ch...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27413-1
更新日期:2018-06-13 00:00:00
abstract::Our previous studies demonstrated that volatile oil from saussurea lappa root (VOSL), rich in two natural sesquiterpene lactones, costunolide (Cos) and dehydrocostuslactone (Dehy), exerts better anti-breast cancer efficacy and lower side effects than Cos or Dehy alone in vivo, however, their anti-cancer molecular mech...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41254
更新日期:2017-01-24 00:00:00