Single cell profiling of capillary blood enables out of clinic human immunity studies.

Abstract:

:An individual's immune system is driven by both genetic and environmental factors that vary over time. To better understand the temporal and inter-individual variability of gene expression within distinct immune cell types, we developed a platform that leverages multiplexed single-cell sequencing and out-of-clinic capillary blood extraction to enable simplified, cost-effective profiling of the human immune system across people and time at single-cell resolution. Using the platform, we detect widespread differences in cell type-specific gene expression between subjects that are stable over multiple days.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Dobreva T,Brown D,Park JH,Thomson M

doi

10.1038/s41598-020-77073-3

subject

Has Abstract

pub_date

2020-11-25 00:00:00

pages

20540

issue

1

issn

2045-2322

pii

10.1038/s41598-020-77073-3

journal_volume

10

pub_type

杂志文章
  • Hidden Hypocalcemia as a Risk Factor for Cardiovascular Events and All-Cause Mortality among Patients Undergoing Incident Hemodialysis.

    abstract::Lower corrected calcium (cCa) levels are associated with a better prognosis among incident dialysis patients. However, cCa frequently overestimates ionized calcium (iCa) levels. The prognostic importance of the true calcium status defined by iCa remains to be revealed. We conducted a retrospective cohort study of inci...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-61459-4

    authors: Yamaguchi S,Hamano T,Doi Y,Oka T,Kajimoto S,Kubota K,Yasuda S,Shimada K,Matsumoto A,Hashimoto N,Sakaguchi Y,Matsui I,Isaka Y

    更新日期:2020-03-10 00:00:00

  • A new perspective in understanding rainfall from satellites over a complex topographic region of India.

    abstract::Present study focuses on rainfall over Western Ghats (WG), a complex topographic region (elevation > 500 m) of India to evaluate and to better understand the satellite behavior in contrast with a flat region (FR) (elevation < 500 m) of central India from 1998 to 2016 using the combinatory data sets of TMPA and IMERG (...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-52075-y

    authors: Thakur MK,Kumar TVL,Koteswara Rao K,Barbosa H,Rao VB

    更新日期:2019-10-30 00:00:00

  • Key role of 15-LO/15-HETE in angiogenesis and functional recovery in later stages of post-stroke mice.

    abstract::This study sought to clarify the effects of 15-lipoxygenase/15-hydroxyeicosatetraenoic acid in angiogenesis and neurological functional recovery after cerebral ischaemic stroke in mice. In vivo, we performed behavioural tests to determine functional recovery after stroke. Double immunofluorescence staining of CD31 and...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep46698

    authors: Wang D,Liu Y,Chen L,Li P,Qu Y,Zhu Y,Zhu Y

    更新日期:2017-04-24 00:00:00

  • Spatial Distribution of the Cannabinoid Type 1 and Capsaicin Receptors May Contribute to the Complexity of Their Crosstalk.

    abstract::The cannabinoid type 1 (CB1) receptor and the capsaicin receptor (TRPV1) exhibit co-expression and complex, but largely unknown, functional interactions in a sub-population of primary sensory neurons (PSN). We report that PSN co-expressing CB1 receptor and TRPV1 form two distinct sub-populations based on their pharmac...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep33307

    authors: Chen J,Varga A,Selvarajah S,Jenes A,Dienes B,Sousa-Valente J,Kulik A,Veress G,Brain SD,Baker D,Urban L,Mackie K,Nagy I

    更新日期:2016-09-22 00:00:00

  • A novel orally bioavailable compound KPT-9274 inhibits PAK4, and blocks triple negative breast cancer tumor growth.

    abstract::Breast cancer is a heterogeneous disease consisting of several subtypes. Among these subtypes, triple negative breast cancer is particularly difficult to treat. This is due to a lack of understanding of the mechanisms behind the disease, and consequently a lack of druggable targets. PAK4 plays critical roles in cell s...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep42555

    authors: Rane C,Senapedis W,Baloglu E,Landesman Y,Crochiere M,Das-Gupta S,Minden A

    更新日期:2017-02-15 00:00:00

  • The Plasmodiophora brassicae genome reveals insights in its life cycle and ancestry of chitin synthases.

    abstract::Plasmodiophora brassicae causes clubroot, a major disease of Brassica oil and vegetable crops worldwide. P. brassicae is a Plasmodiophorid, obligate biotrophic protist in the eukaryotic kingdom of Rhizaria. Here we present the 25.5 Mb genome draft of P. brassicae, developmental stage-specific transcriptomes and a tran...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep11153

    authors: Schwelm A,Fogelqvist J,Knaust A,Jülke S,Lilja T,Bonilla-Rosso G,Karlsson M,Shevchenko A,Dhandapani V,Choi SR,Kim HG,Park JY,Lim YP,Ludwig-Müller J,Dixelius C

    更新日期:2015-06-18 00:00:00

  • Effects of Lead and Mercury on Sulfate-Reducing Bacterial Activity in a Biological Process for Flue Gas Desulfurization Wastewater Treatment.

    abstract::Biological sulfate-reducing bacteria (SRB) may be effective in removing toxic lead and mercury ions (Pb(II) and Hg(II)) from wet flue gas desulfurization (FGD) wastewater through anaerobic sulfite reduction. To confirm this hypothesis, a sulfite-reducing up-flow anaerobic sludge blanket reactor was set up to treat FGD...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep30455

    authors: Zhang L,Lin X,Wang J,Jiang F,Wei L,Chen G,Hao X

    更新日期:2016-07-26 00:00:00

  • Scaling and correlation properties of RR and QT intervals at the cellular level.

    abstract::We study complex scaling properties of RR and QT intervals of electrocardiograms (ECGs) with their equivalences at the cellular level, that is, inter-beat intervals (IBI) and field potential durations (FPD) of spontaneously beating human-induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) aggregates. Our de...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-40247-9

    authors: Kim J,Shah D,Potapov I,Latukka J,Aalto-Setälä K,Räsänen E

    更新日期:2019-03-06 00:00:00

  • A novel approach for discovering stochastic models behind data applied to El Niño-Southern Oscillation.

    abstract::Stochastic differential equations (SDEs) are ubiquitous across disciplines, and uncovering SDEs driving observed time series data is a key scientific challenge. Most previous work on this topic has relied on restrictive assumptions, undermining the generality of these approaches. We present a novel technique to uncove...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-021-81162-2

    authors: Olson R,An SI,Kim SK,Fan Y

    更新日期:2021-01-29 00:00:00

  • Structural and functional characterization of M. tuberculosis sedoheptulose- 7-phosphate isomerase, a critical enzyme involved in lipopolysaccharide biosynthetic pathway.

    abstract::M. tuberculosis GmhA enzyme catalyzes the isomerization of D-sedoheptulose 7-phosphate into D-glycero-D-α-manno-heptose-7-phosphate in GDP-D-glycero-α-D-manno-heptose biosynthetic pathway. The D-glycero-α-D-manno-heptose is a major constituent of lipopolysaccharide and contributes to virulence and antibiotic resistanc...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-77230-8

    authors: Karan S,Pratap B,Yadav SP,Ashish F,Saxena AK

    更新日期:2020-11-30 00:00:00

  • Ultralow Lattice Thermal Conductivity of the Random Multilayer Structure with Lattice Imperfections.

    abstract::Randomizing the layer thickness of superlattices (SL) can lead to localization of coherent phonons and thereby reduces the lattice thermal conductivity κ l . In this work, we propose strategies that can suppress incoherent phonon transport in the above random multilayer (RML) structure to further reduce κ l . Molecula...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-08359-2

    authors: Chakraborty P,Cao L,Wang Y

    更新日期:2017-08-15 00:00:00

  • Modelling water levels of northwestern India in response to improved irrigation use efficiency.

    abstract::The groundwater crisis in northwestern India is the result of over-exploitation of groundwater resources for irrigation. The Government of India has targeted a 20 percent improvement in irrigation groundwater use efficiency. In this perspective, and using a regional-scale calibrated and validated three-dimensional gro...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-70416-0

    authors: Shekhar S,Kumar S,Densmore AL,van Dijk WM,Sinha R,Kumar M,Joshi SK,Rai SP,Kumar D

    更新日期:2020-08-10 00:00:00

  • A prospective, randomised study of the effect of fixation sutures during phacotrabeculectomy on intraocular pressure and incidence of ptosis.

    abstract::We investigated the effects of different intraoperative eyeball fixation techniques (superior rectus muscle suture [MS] and traction suture at the corneal limbus [CS]), on intraocular pressure (IOP) and the incidence of ptosis after phacotrabeculectomy. Forty-one eyes with different glaucoma types which qualified for ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-79635-x

    authors: Konopińska J,Lisowski Ł,Mariak Z,Wojnar M,Obuchowska I,Rękas M

    更新日期:2021-01-12 00:00:00

  • Sporadic colon cancer murine models demonstrate the value of autoantibody detection for preclinical cancer diagnosis.

    abstract::Although autoantibody detection has been proposed for diagnosis of colorectal cancer, little is known about their initial production and development correlation with cancer progression. Azoxymethane/dextran sodium sulfate (AOM/DSS)-treated mice developed colon adenocarcinoma in the distal colon similar to human sporad...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep02938

    authors: Barderas R,Villar-Vázquez R,Fernández-Aceñero MJ,Babel I,Peláez-García A,Torres S,Casal JI

    更新日期:2013-10-15 00:00:00

  • Potential use of mealworm frass as a fertilizer: Impact on crop growth and soil properties.

    abstract::Rearing insects is expected to dramatically increase during the next few years, and this will be associated with generating high quantities of frass (insect excreta). It is necessary to find solutions allowing the efficient valorization of these by-products before a major upscaling of the industry takes place. Therefo...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-61765-x

    authors: Houben D,Daoulas G,Faucon MP,Dulaurent AM

    更新日期:2020-03-13 00:00:00

  • Mesoporous Platinum Prepared by Electrodeposition for Ultralow Loading Proton Exchange Membrane Fuel Cells.

    abstract::The porosity and utilization of platinum catalysts have a direct impact on their performance within proton exchange membrane fuel cells. It is desirable to identify methods that can prepare these catalysts with the desired features, and that can be widely implemented using existing and industrially scalable techniques...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-38855-6

    authors: Paul MTY,Gates BD

    更新日期:2019-03-11 00:00:00

  • Precoce and opposite response of proteasome activity after acute or chronic exposure of C. elegans to γ-radiation.

    abstract::Species are chronically exposed to ionizing radiation, a natural phenomenon which can be enhanced by human activities. The induced toxicity mechanisms still remain unclear and seem depending on the mode of exposure, i.e. acute and chronic. To better understand these phenomena, studies need to be conducted both at the ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-29033-1

    authors: Dubois C,Lecomte C,Ruys SPD,Kuzmic M,Della-Vedova C,Dubourg N,Galas S,Frelon S

    更新日期:2018-07-27 00:00:00

  • Postnatal development of the small intestinal mucosa drives age-dependent, regio-selective susceptibility to Escherichia coli K1 infection.

    abstract::The strong age dependency of neonatal systemic infection with Escherichia coli K1 can be replicated in the neonatal rat. Gastrointestinal (GI) colonization of two-day-old (P2) rats leads to invasion of the blood within 48 h of initiation of colonization; pups become progressively less susceptible to infection over the...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-00123-w

    authors: Birchenough GM,Dalgakiran F,Witcomb LA,Johansson ME,McCarthy AJ,Hansson GC,Taylor PW

    更新日期:2017-03-06 00:00:00

  • Gasoline cars produce more carbonaceous particulate matter than modern filter-equipped diesel cars.

    abstract::Carbonaceous particulate matter (PM), comprising black carbon (BC), primary organic aerosol (POA) and secondary organic aerosol (SOA, from atmospheric aging of precursors), is a highly toxic vehicle exhaust component. Therefore, understanding vehicle pollution requires knowledge of both primary emissions, and how thes...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-03714-9

    authors: Platt SM,El Haddad I,Pieber SM,Zardini AA,Suarez-Bertoa R,Clairotte M,Daellenbach KR,Huang RJ,Slowik JG,Hellebust S,Temime-Roussel B,Marchand N,de Gouw J,Jimenez JL,Hayes PL,Robinson AL,Baltensperger U,Astorga C,Prévô

    更新日期:2017-07-13 00:00:00

  • Interactive effect of sleep duration and sleep quality on risk of stroke: An 8-year follow-up study in China.

    abstract::Inappropriate sleep duration and poor sleep quality are associated with risk of stroke, but their interactive effect on stroke is unknown. We explored the interactive effect of sleep quality and duration on stroke risk. A prospective cohort study was conducted with 41,786 adults. Sleep quality was assessed using the P...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-65611-y

    authors: Ji A,Lou H,Lou P,Xu C,Zhang P,Qiao C,Yang Q

    更新日期:2020-05-26 00:00:00

  • Publisher Correction: AKT-mTOR signaling modulates the dynamics of IRE1 RNAse activity by regulating ER-mitochondria contacts.

    abstract::A correction to this article has been published and is linked from the HTML version of this paper. The error has not been fixed in the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-018-24796-z

    authors: Sanchez-Alvarez M,Del Pozo MA,Bakal C

    更新日期:2018-04-19 00:00:00

  • TLR4 counteracts BVRA signaling in human leukocytes via differential regulation of AMPK, mTORC1 and mTORC2.

    abstract::TLR4 is implicated in diseases associated with chronic low-grade inflammation, yet homeostatic signaling mechanisms that prevent and/or are affected by chronic TLR4 activation are largely uncharacterized. We recently reported that LPS/TLR4 activates in human leukocytes signaling intermediates (SI), abbreviated TLR4-SI...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-43347-8

    authors: Zhang Z,Amorosa LF,Petrova A,Coyle S,Macor M,Nair M,Lee LY,Haimovich B

    更新日期:2019-05-07 00:00:00

  • Evolution of deceptive and true courtship songs in moths.

    abstract::Ultrasonic mating signals in moths are argued to have evolved via exploitation of the receivers' sensory bias towards bat echolocation calls. We have demonstrated that female moths of the Asian corn borer are unable to distinguish between the male courtship song and bat calls. Females react to both the male song and b...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep02003

    authors: Nakano R,Takanashi T,Surlykke A,Skals N,Ishikawa Y

    更新日期:2013-01-01 00:00:00

  • Full annual monitoring of Subantarctic Emiliania huxleyi populations reveals highly calcified morphotypes in high-CO2 winter conditions.

    abstract::Ocean acidification is expected to have detrimental consequences for the most abundant calcifying phytoplankton species Emiliania huxleyi. However, this assumption is mainly based on laboratory manipulations that are unable to reproduce the complexity of natural ecosystems. Here, E. huxleyi coccolith assemblages colle...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-59375-8

    authors: Rigual-Hernández AS,Trull TW,Flores JA,Nodder SD,Eriksen R,Davies DM,Hallegraeff GM,Sierro FJ,Patil SM,Cortina A,Ballegeer AM,Northcote LC,Abrantes F,Rufino MM

    更新日期:2020-02-13 00:00:00

  • NKG2D+CD4+ T Cells Kill Regulatory T Cells in a NKG2D-NKG2D Ligand- Dependent Manner in Systemic Lupus Erythematosus.

    abstract::Systemic lupus erythematosus (SLE) features a decreased pool of CD4+CD25+Foxp3+ T regulatory (Treg) cells. We had previously observed NKG2D+CD4+ T cell expansion in contrast to a decreased pool of Treg cells in SLE patients, but whether NKG2D+CD4+ T cells contribute to the decreased Treg cells remains unclear. In the ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-01379-y

    authors: Yang D,Tian Z,Zhang M,Yang W,Tang J,Wu Y,Ni B

    更新日期:2017-04-28 00:00:00

  • Physico-chemical properties based differential toxicity of graphene oxide/reduced graphene oxide in human lung cells mediated through oxidative stress.

    abstract::Graphene derivatives (GD) are currently being evaluated for technological and biomedical applications owing to their unique physico-chemical properties over other carbon allotrope such as carbon nanotubes (CNTs). But, the possible association of their properties with underlying in vitro effects have not fully examined...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep39548

    authors: Mittal S,Kumar V,Dhiman N,Chauhan LK,Pasricha R,Pandey AK

    更新日期:2016-12-21 00:00:00

  • Mitochondrial behaviour throughout the lytic cycle of Toxoplasma gondii.

    abstract::Mitochondria distribution in cells controls cellular physiology in health and disease. Here we describe the mitochondrial morphology and positioning found in the different stages of the lytic cycle of the eukaryotic single-cell parasite Toxoplasma gondii. The lytic cycle, driven by the tachyzoite life stage, is respon...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep42746

    authors: Ovciarikova J,Lemgruber L,Stilger KL,Sullivan WJ,Sheiner L

    更新日期:2017-02-16 00:00:00

  • Adjusted Troponin I for Improved Evaluation of Patients with Chest Pain.

    abstract::The use of cardiac troponins (cTn) is the gold standard for diagnosing myocardial infarction. Independent of myocardial infarction (MI), however, sex, age and kidney function affect cTn levels. Here we developed a method to adjust cTnI levels for age, sex, and renal function, maintaining a unified cut-off value such a...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-26120-1

    authors: Boeckel JN,Palapies L,Klotsche J,Zeller T,von Jeinsen B,Perret MF,Kleinhaus SL,Pieper L,Tzikas S,Leistner D,Bickel C,Stalla GK,Lehnert H,Lindahl B,Wittchen HU,Silber S,Baldus S,Maerz W,Dimmeler S,Blankenberg S,Mün

    更新日期:2018-05-24 00:00:00

  • Photonic transistor and router using a single quantum-dot-confined spin in a single-sided optical microcavity.

    abstract::The future Internet is very likely the mixture of all-optical Internet with low power consumption and quantum Internet with absolute security guaranteed by the laws of quantum mechanics. Photons would be used for processing, routing and com-munication of data, and photonic transistor using a weak light to control a st...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep45582

    authors: Hu CY

    更新日期:2017-03-28 00:00:00

  • Apparent Effects of Opioid Use on Neural Responses to Reward in Chronic Pain.

    abstract::Neural responses to incentives are altered in chronic pain and by opioid use. To understand how opioid use modulates the neural response to reward/value in chronic pain, we compared brain functional magnetic resonance imaging (fMRI) responses to a monetary incentive delay (MID) task in patients with fibromyalgia takin...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-45961-y

    authors: Martucci KT,MacNiven KH,Borg N,Knutson B,Mackey SC

    更新日期:2019-07-03 00:00:00