Abstract:
:Soluble envelope (Env) trimers, stabilized in a prefusion-closed conformation, can elicit neutralizing responses against HIV-1 strains closely related to the immunizing trimer. However, to date such stabilization has succeeded with only a limited number of HIV-1 strains. To address this issue, here we develop ADROITrimer, an automated procedure involving structure-based stabilization and consensus repair, and generate "RnS-DS-SOSIP"-stabilized Envs from 180 diverse Env sequences. The vast majority of these RnS-DS-SOSIP Envs fold into prefusion-closed conformations as judged by antigenic analysis and size exclusion chromatography. Additionally, representative strains from clades AE, B, and C are stabilized in prefusion-closed conformations as shown by negative-stain electron microscopy, and the crystal structure of a clade A strain MI369.A5 Env trimer provides 3.5 Å resolution detail into stabilization and repair mutations. The automated procedure reported herein that yields well-behaved, soluble, prefusion-closed Env trimers from a majority of HIV-1 strains could have substantial impact on the development of an HIV-1 vaccine.
journal_name
Cell Repjournal_title
Cell reportsauthors
Rawi R,Rutten L,Lai YT,Olia AS,Blokland S,Juraszek J,Shen CH,Tsybovsky Y,Verardi R,Yang Y,Zhang B,Zhou T,Chuang GY,Kwong PD,Langedijk JPMdoi
10.1016/j.celrep.2020.108432subject
Has Abstractpub_date
2020-11-24 00:00:00pages
108432issue
8issn
2211-1247pii
S2211-1247(20)31421-2journal_volume
33pub_type
杂志文章相关文献
Cell Reports文献大全abstract::The chemokine receptor CCR7 plays a crucial role in the homing of central memory and naive T cells to peripheral lymphoid organs. Here, we show that the HIV-1 accessory protein Vpu downregulates CCR7 on the surface of CD4(+) T cells. Vpu and CCR7 were found to specifically interact and colocalize within the trans-Golg...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.05.015
更新日期:2014-06-26 00:00:00
abstract::Interest in manipulating the gut microbiota to treat disease has led to a need for understanding how organisms can establish themselves when introduced into a host with an intact microbial community. Here, we employ the concept of orthogonal niche engineering: a resource typically absent from the diet, seaweed, create...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.07.032
更新日期:2018-08-14 00:00:00
abstract::Clinical transplantation of tissue fragments, including islets, faces a critical challenge because of a lack of effective strategies that ensure efficient engraftment through the timely integration of vascular networks. We recently developed a complex organoid engineering method by "self-condensation" culture based on...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.03.123
更新日期:2018-05-08 00:00:00
abstract::Gli proteins are transcriptional effectors of the Hedgehog (Hh) pathway in both normal development and cancer. We describe a program of multisite phosphorylation that regulates the conversion of Gli proteins into transcriptional activators. In the absence of Hh ligands, Gli activity is restrained by the direct phospho...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2013.12.003
更新日期:2014-01-16 00:00:00
abstract::Leptin has beneficial effects on glucose metabolism via actions in the hypothalamus, but the roles of specific subgroups of neurons responsible for these antidiabetic effects remain unresolved. We generated diabetic Lep(ob/ob) or Lepr(db/db) mice lacking or re-expressing leptin receptors (LepRb) in subgroups of neuron...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.04.010
更新日期:2014-05-22 00:00:00
abstract::To ensure a successful infection, herpesviruses have developed elegant strategies to counterbalance the host anti-viral responses. Sterile alpha motif and HD domain 1 (SAMHD1) was recently identified as an intrinsic restriction factor for a variety of viruses. Aside from HIV-2 and the related simian immunodeficiency v...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.04.020
更新日期:2019-07-09 00:00:00
abstract::Recurrent copy-number alterations, mutations, and transcript fusions of the genes encoding CDKs/cyclins are characterized in >10,000 tumors. Genomic alterations of CDKs/cyclins are dominantly driven by copy number aberrations. In contrast to cell-cycle-related CDKs/cyclins, which are globally amplified, transcriptiona...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.107884
更新日期:2020-07-14 00:00:00
abstract::Lineage analysis of autoreactive B cells can reveal the origins of autoimmunity. In the autoimmune disease pemphigus vulgaris (PV), desmoglein 3 (DSG3) and DSG1 autoantibodies are predominantly of the IgG4 subclass and less frequently of IgG1 and IgA subclasses, prompting us to investigate whether anti-DSG IgG4 B cell...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.07.093
更新日期:2018-08-28 00:00:00
abstract::Spontaneous neurotransmitter release is a core element of synaptic communication in mature neurons, but despite exceptionally high levels of spontaneous vesicle cycling occurring in developing axons, little is known of its function during this period. We now show that high-level, spontaneous axonal release of the neur...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.01.032
更新日期:2015-02-17 00:00:00
abstract::The quest to extend healthspan via pharmacological means is becoming increasingly urgent, both from a health and economic perspective. Here we show that lithium, a drug approved for human use, promotes longevity and healthspan. We demonstrate that lithium extends lifespan in female and male Drosophila, when administer...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.03.041
更新日期:2016-04-19 00:00:00
abstract::During development, neurons are constantly refining their connections in response to changes in activity. Experience-dependent plasticity is a key form of synaptic plasticity, involving changes in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) accumulation at synapses. Here, we report a critical...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.04.054
更新日期:2014-06-12 00:00:00
abstract::Cytotoxic CD8(+) T lymphocytes play a critical role in the host response to infection by viruses. The ability to secrete cytotoxic chemicals and cytokines is considered pivotal for eliminating virus. Of equal importance is how effector CD8(+) T cells home to virus-infected tissues. L-selectin has not been considered i...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.12.090
更新日期:2016-02-02 00:00:00
abstract::Complex architectural rearrangements are associated to the control of the HoxD genes in different cell types; yet, how they are implemented in single cells remains unknown. By use of polymer models, we dissect the locus 3D structure at the single DNA molecule level in mouse embryonic stem and cortical neuronal cells, ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.07.013
更新日期:2019-08-06 00:00:00
abstract::Although it is well established that tumor-associated macrophages take part in each step of cancer progression, less is known about the distinct role of the so-called metastasis-associated macrophages (MAMs) at the metastatic site. Previous studies reported that Caveolin-1 (Cav1) has both tumor-promoting and tumor-sup...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.11.034
更新日期:2017-12-05 00:00:00
abstract::Squamous cell carcinoma (SCC) of the lung is the second most common subtype of lung cancer. With limited treatment options, the 5-year survival rate of SCC is only 15%. Although genomic alterations in SCC have been characterized, identifying the alterations that drive SCC is critical for improving treatment strategies...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.05.036
更新日期:2014-07-10 00:00:00
abstract::The ability to link events that are separated in time is important for extracting meaning from experiences and guiding behavior in the future. This ability likely requires the brain to continue representing events even after they have passed, a process that may involve the prefrontal cortex and takes the form of susta...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.05.021
更新日期:2016-06-14 00:00:00
abstract::The molecular basis of higher regenerative capacity of cold-blooded animals comparing to warm-blooded ones is poorly understood. Although this difference in regenerative capacities is commonly thought to be a result of restructuring of the same regulatory gene network, we hypothesized that it may be due to loss of som...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.09.038
更新日期:2019-10-22 00:00:00
abstract::EEG reverse-correlation techniques have revealed that visual information processing entails a ∼10-Hz (alpha) occipital response that reverberates sensory inputs up to 1 s. However, the spatial distribution of these perceptual echoes remains unknown: are they synchronized across the brain, or do they propagate like a t...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.12.058
更新日期:2019-01-08 00:00:00
abstract::The Piwi-piRNA pathway is well known for its germline function, yet its somatic role remains elusive. We show here that Piwi is required autonomously not only for germline stem cell (GSC) but also for somatic cyst stem cell (CySC) maintenance in the Drosophila testis. Reducing Piwi activity in the testis caused defect...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.06.004
更新日期:2015-07-07 00:00:00
abstract::Self-renewal is a key characteristic of leukemia stem cells (LSCs) responsible for the development and maintenance of leukemia. In this study, we identify CD93 as an important regulator of self-renewal and proliferation of murine and human LSCs, but not hematopoietic stem cells (HSCs). The intracellular domain of CD93...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108663
更新日期:2021-01-26 00:00:00
abstract::Glioblastoma (GBM) is highly refractory to therapy and associated with poor clinical outcome. Here, we reveal a critical function of the promitotic and adhesion-mediating discoidin domain receptor 1 (DDR1) in modulating GBM therapy resistance. In GBM cultures and clinical samples, we show a DDR1 and GBM stem cell mark...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.02.096
更新日期:2019-03-26 00:00:00
abstract::Virus infection is sensed by pattern recognition receptors (PRRs) detecting virus nucleic acids and initiating an innate immune response. DNA-dependent protein kinase (DNA-PK) is a PRR that binds cytosolic DNA and is antagonized by vaccinia virus (VACV) protein C16. Here, VACV protein C4 is also shown to antagonize DN...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.10.034
更新日期:2018-11-13 00:00:00
abstract::The intestinal tract is constantly exposed to various stimuli. Group 3 innate lymphoid cells (ILC3s) reside in lymphoid organs and in the intestinal tract and are required for immunity to enteric bacterial infection. However, the mechanisms that regulate the ILC3s in vivo remain incompletely defined. Here, we show tha...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.05.099
更新日期:2018-06-26 00:00:00
abstract::Two hallmarks of cancer cells are their resistance to apoptosis and ability to thrive despite reduced levels of vital serum components. c-jun N-terminal kinase (JNK) activation is crucial for apoptosis triggered by serum starvation (SS), and isocitrate dehydrogenase 1 (IDH1) mutations are tumorigenic, in part, because...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.03.053
更新日期:2017-04-11 00:00:00
abstract::Atrophy of neurons in the prefrontal cortex (PFC) plays a key role in the pathophysiology of depression and related disorders. The ability to promote both structural and functional plasticity in the PFC has been hypothesized to underlie the fast-acting antidepressant properties of the dissociative anesthetic ketamine....
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.05.022
更新日期:2018-06-12 00:00:00
abstract::Mammalian neocortex size primarily reflects the number and mode of divisions of neural stem and progenitor cells. Cortical stem cells (apical progenitors) switching from symmetric divisions, which expand their population, to asymmetric divisions, which generate downstream neuronal progenitors (basal progenitors), star...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.03.033
更新日期:2014-04-24 00:00:00
abstract::CD4 and CD8 T cells play a pivotal role in controlling tumor growth. However, the interplay of both cell types and their role in tumor suppression still remain elusive. In this study, we investigated the regulation of CD4 and CD8 T cell responses to different classes of tumor-specific antigens in liver cancer mouse mo...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.10.086
更新日期:2016-11-22 00:00:00
abstract::Information gained during goal pursuit motivates adaptive behavior. The anterior cingulate cortex (ACC) supports adaptive behavior, but how ACC signals are translated into motivational signals remains unclear. Rats with implants in the ACC and ventral tegmental area (VTA), a dopaminergic brain area implicated in motiv...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.05.062
更新日期:2017-06-13 00:00:00
abstract::Hypertrophic white adipose tissue (WAT) represents a maladaptive mechanism linked to the risk for developing type 2 diabetes in humans. However, the molecular events that predispose WAT to hypertrophy are poorly defined. Here, we demonstrate that adipocyte hypertrophy is triggered by loss of the corepressor GPS2 durin...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.08.032
更新日期:2018-09-11 00:00:00
abstract::While the I kappa kinase (IKK) scaffolding protein NF-κB essential modulator (NEMO) binds to polyubiquitin chains to transmit inflammatory signals, NEMO itself is also ubiquitinated in response to a variety of inflammatory agonists. Although there have been hints that polyubiquitination of NEMO is essential for avoidi...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2013.06.036
更新日期:2013-07-25 00:00:00