Transcriptome analyses reveals the dynamic nature of oil accumulation during seed development of Plukenetia volubilis L.

Abstract:

:Sacha inchi (Plukenetia volubilis L.) is a shrub native to Amazon rainforests that's of commercial interest as its seeds contain 35-60% edible oil (dry weight). This oil is one of the healthiest vegetable oils due to its high polyunsaturated fatty acid content and favourable ratio of omega-6 to omega-3 fatty acids. De novo transcriptome assembly and comparative analyses were performed on sacha inchi seeds from five stages of seed development in order to identifying genes associated with oil accumulation and fatty acid production. Of 30,189 unigenes that could be annotated in public databases, 20,446 were differentially expressed unigenes. A total of 14 KEGG pathways related to lipid metabolism were found, and 86 unigenes encoding enzymes involved in α-linolenic acid (ALA) biosynthesis were obtained including five unigenes encoding FATA (Unigene0008403), SAD (Unigene0012943), DHLAT (Unigene0014324), α-CT (Unigene0022151) and KAS II (Unigene0024371) that were significantly up-regulated in the final stage of seed development. A total of 66 unigenes encoding key enzymes involved in the synthesis of triacylglycerols (TAGs) were found, along with seven unigenes encoding PDCT (Unigene0000909), LPCAT (Unigene0007846), Oleosin3 (Unigene0010027), PDAT1 (Unigene0016056), GPDH (Unigene0022660), FAD2 (Unigene0037808) and FAD3 (Unigene0044238); these also proved to be up-regulated in the final stage of seed development.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Liu G,Wu Z,Peng Y,Shang X,Xie Y,Arnold RJ

doi

10.1038/s41598-020-77177-w

subject

Has Abstract

pub_date

2020-11-24 00:00:00

pages

20467

issue

1

issn

2045-2322

pii

10.1038/s41598-020-77177-w

journal_volume

10

pub_type

杂志文章
  • Genomic insights into the population structure and history of the Irish Travellers.

    abstract::The Irish Travellers are a population with a history of nomadism; consanguineous unions are common and they are socially isolated from the surrounding, 'settled' Irish people. Low-resolution genetic analysis suggests a common Irish origin between the settled and the Traveller populations. What is not known, however, i...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep42187

    authors: Gilbert E,Carmi S,Ennis S,Wilson JF,Cavalleri GL

    更新日期:2017-02-09 00:00:00

  • Historical and Projected Surface Temperature over India during the 20th and 21st century.

    abstract::Surface Temperature (ST) over India has increased by ~0.055 K/decade during 1860-2005 and follows the global warming trend. Here, the natural and external forcings (e.g., natural and anthropogenic) responsible for ST variability are studied from Coupled Model Inter-comparison phase 5 (CMIP5) models during the 20th cen...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-02130-3

    authors: Basha G,Kishore P,Ratnam MV,Jayaraman A,Agha Kouchak A,Ouarda TBMJ,Velicogna I

    更新日期:2017-06-07 00:00:00

  • Inter-digital capacitors with aerosol-deposited high-K dielectric layer for highest capacitance value in capacitive super-sensing applications.

    abstract::Inter-digital capacitors (IDCs) with aerosol-deposition (AD) high-k dielectric layer were compared via simulation and measurements of bare IDCs and AD IDCs at room temperature and subjected to a post-annealing process for realizing capacitive super-sensing applications. IDCs with thin AD films can provide higher capac...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-37416-7

    authors: Kim ES,Liang JG,Wang C,Cho MY,Oh JM,Kim NY

    更新日期:2019-01-24 00:00:00

  • CT changes of severe coronavirus disease 2019 based on prognosis.

    abstract::This study aimed to determine the characteristics of CT changes in patients with severe coronavirus disease 2019 (COVID-19) based on prognosis. Serial CT scans in 47 patients with severe COVID-19 were reviewed. The patterns, distribution and CT score of lung abnormalities were assessed. Scans were classified according...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-78965-0

    authors: Liang B,Xie L,Yang F,Makamure J,Zhang L,Pang R,Du P,Fan W,Zheng C

    更新日期:2020-12-14 00:00:00

  • A unique clade of light-driven proton-pumping rhodopsins evolved in the cyanobacterial lineage.

    abstract::Microbial rhodopsin is a photoreceptor protein found in various bacteria and archaea, and it is considered to be a light-utilization device unique to heterotrophs. Recent studies have shown that several cyanobacterial genomes also include genes that encode rhodopsins, indicating that these auxiliary light-utilizing pr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-73606-y

    authors: Hasegawa M,Hosaka T,Kojima K,Nishimura Y,Nakajima Y,Kimura-Someya T,Shirouzu M,Sudo Y,Yoshizawa S

    更新日期:2020-10-07 00:00:00

  • Soil microbial carbon utilization, enzyme activities and nutrient availability responses to Bidens pilosa and a non-invasive congener under different irradiances.

    abstract::Two Bidens species (Bidens pilosa and B. bipinnata) that originate from America have been introduced widely in pan-tropics, with the former regarded as a noxious invasive weed whereas the latter naturalized as a plant resource. Whether the two species exhibit different effects on the belowground system remains rarely ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-11707-x

    authors: Wei H,Yan W,Quan G,Zhang J,Liang K

    更新日期:2017-09-12 00:00:00

  • Visual cortex and cerebellum hyperactivation during negative emotion picture stimuli in migraine patients.

    abstract::Migraines are a common and undertreated disease and often have psychiatric comorbidities; however, the abnormal mechanism of emotional processing in migraine patients has not been well clarified. This study sought to investigate the different brain functional activation to neutral, positive and negative emotional stim...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep41919

    authors: Wang M,Su J,Zhang J,Zhao Y,Yao Q,Zhang Q,Zhang H,Wang S,Li GF,Liu JR,Du X

    更新日期:2017-02-09 00:00:00

  • Enhancement of Environmental Hazard Degradation in the Presence of Lignin: a Proteomics Study.

    abstract::Proteomics studies of fungal systems have progressed dramatically based on the availability of more fungal genome sequences in recent years. Different proteomics strategies have been applied toward characterization of fungal proteome and revealed important gene functions and proteome dynamics. Presented here is the ap...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-10132-4

    authors: Sun S,Xie S,Cheng Y,Yu H,Zhao H,Li M,Li X,Zhang X,Yuan JS,Dai SY

    更新日期:2017-09-12 00:00:00

  • The effect of ionic strength on oil adhesion in sandstone--the search for the low salinity mechanism.

    abstract::Core flood and field tests have demonstrated that decreasing injection water salinity increases oil recovery from sandstone reservoirs. However, the microscopic mechanism behind the effect is still under debate. One hypothesis is that as salinity decreases, expansion of the electrical double layer decreases attraction...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep09933

    authors: Hilner E,Andersson MP,Hassenkam T,Matthiesen J,Salino PA,Stipp SL

    更新日期:2015-04-22 00:00:00

  • CLE peptide-encoding gene families in Medicago truncatula and Lotus japonicus, compared with those of soybean, common bean and Arabidopsis.

    abstract::CLE peptide hormones are critical regulators of many cell proliferation and differentiation mechanisms in plants. These 12-13 amino acid glycosylated peptides play vital roles in a diverse range of plant tissues, including the shoot, root and vasculature. CLE peptides are also involved in controlling legume nodulation...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-09296-w

    authors: Hastwell AH,de Bang TC,Gresshoff PM,Ferguson BJ

    更新日期:2017-08-24 00:00:00

  • Long-range inputome of cortical neurons containing corticotropin-releasing hormone.

    abstract::Dissection of the neural circuits of the cerebral cortex is essential for studying mechanisms underlying brain function. Herein, combining a retrograde rabies tracing system with fluorescent micro-optical sectional tomography, we investigated long-range input neurons of corticotropin-releasing hormone containing neuro...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-68115-x

    authors: Zhao P,Zhao M,Wang H,Jiang T,Jia X,Tian J,Li A,Gong H,Li X

    更新日期:2020-07-22 00:00:00

  • Radiomics prognostication model in glioblastoma using diffusion- and perfusion-weighted MRI.

    abstract::We aimed to develop and validate a multiparametric MR radiomics model using conventional, diffusion-, and perfusion-weighted MR imaging for better prognostication in patients with newly diagnosed glioblastoma. A total of 216 patients with newly diagnosed glioblastoma were enrolled from two tertiary medical centers and...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-61178-w

    authors: Park JE,Kim HS,Jo Y,Yoo RE,Choi SH,Nam SJ,Kim JH

    更新日期:2020-03-06 00:00:00

  • Usefulness of continuous probability distributions of rates for modelling radionuclide biokinetics in humans and animals.

    abstract::Modelling the biokinetics of radionuclide excretion or retention is important in nuclear medicine and following accidental/malicious radioactivity releases. Sums of discrete exponential decay rates are often used, but we hypothesized that continuous probability distributions (CPD) of decay rates can describe the data ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-38046-9

    authors: Shuryak I,Dadachova E

    更新日期:2019-02-04 00:00:00

  • Programmable hydraulic resistor for microfluidic chips using electrogate arrays.

    abstract::Flow rates play an important role in microfluidic devices because they affect the transport of chemicals and determine where and when (bio)chemical reactions occur in these devices. Flow rates can conveniently be determined using external peripherals in active microfluidics. However, setting specific flow rates in pas...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-53885-w

    authors: Salva ML,Temiz Y,Rocca M,Arango YC,Niemeyer CM,Delamarche E

    更新日期:2019-11-21 00:00:00

  • Use of Zebrafish Larvae as a Multi-Endpoint Platform to Characterize the Toxicity Profile of Silica Nanoparticles.

    abstract::Nanomaterials are being extensively produced and applied in society. Human and environmental exposures are, therefore, inevitable and so increased attention is being given to nanotoxicity. While silica nanoparticles (NP) are one of the top five nanomaterials found in consumer and biomedical products, their toxicity pr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep37145

    authors: Pham DH,De Roo B,Nguyen XB,Vervaele M,Kecskés A,Ny A,Copmans D,Vriens H,Locquet JP,Hoet P,de Witte PA

    更新日期:2016-11-22 00:00:00

  • Fetuin-B (FETUB): a Plasma Biomarker Candidate Related to the Severity of Lung Function in COPD.

    abstract::Biomarkers for the progression of lung function in COPD are currently scarce. Plasma fetuin-B (FETUB) was identified by iTRAQ-based proteomics and was verified by ELISA in another group. Information regarding acute exacerbation (AE) was collected in a one-year follow-up programme. FETUB concentrations (1652 ± 427 ng/m...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep30045

    authors: Diao WQ,Shen N,Du YP,Liu BB,Sun XY,Xu M,He B

    更新日期:2016-07-22 00:00:00

  • Loss of Endometrial Sodium Glucose Cotransporter SGLT1 is Detrimental to Embryo Survival and Fetal Growth in Pregnancy.

    abstract::Embryo implantation requires a hospitable uterine environment. A key metabolic change that occurs during the peri-implantation period, and throughout early pregnancy, is the rise in endometrial glycogen content. Glycogen accumulation requires prior cellular uptake of glucose. Here we show that both human and murine en...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-11674-3

    authors: Salker MS,Singh Y,Zeng N,Chen H,Zhang S,Umbach AT,Fakhri H,Kohlhofer U,Quintanilla-Martinez L,Durairaj RRP,Barros FSV,Vrljicak P,Ott S,Brucker SY,Wallwiener D,Vrhovac Madunić I,Breljak D,Sabolić I,Koepsell H,Brosens

    更新日期:2017-10-03 00:00:00

  • Hydrogen segregation and its roles in structural stability and metallization: silane under pressure.

    abstract::We present results from first-principles calculations on silane (SiH4) under pressure. We find that a three dimensional P-3 structure becomes the most stable phase above 241 GPa. A prominent structural feature, which separates the P-3 structure from previously observed/predicted SiH4 structures, is that a fraction of ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep13039

    authors: Cui W,Shi J,Liu H,Yao Y,Wang H,Iitaka T,Ma Y

    更新日期:2015-08-12 00:00:00

  • Laser sintering of gravure printed indium tin oxide films on polyethylene terephthalate for flexible electronics.

    abstract::Tin doped indium oxide (ITO) thin films provide excellent transparency and conductivity for electrodes in displays and photovoltaic systems. Current advances in producing printable ITO inks are reducing the volume of wasted indium during thin film patterning. However, their applicability to flexible electronics is hin...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-38043-y

    authors: Serkov AA,Snelling HV,Heusing S,Amaral TM

    更新日期:2019-02-11 00:00:00

  • In vivo two-photon microscopy of the human eye.

    abstract::Two-photon (2P) microscopy is a powerful tool for imaging and exploring label-free biological tissues at high resolution. Although this type of microscopy has been demonstrated in ex vivo ocular tissues of both humans and animal models, imaging the human eye in vivo has always been challenging. This work presents a no...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-46568-z

    authors: Ávila FJ,Gambín A,Artal P,Bueno JM

    更新日期:2019-07-12 00:00:00

  • Mitochondrial Dysfunction may explain symptom variation in Phelan-McDermid Syndrome.

    abstract::Phelan-McDermid Syndrome (PMS), which is defined by a deletion within 22q13, demonstrates significant phenotypic variation. Given that six mitochondrial genes are located within 22q13, including complex I and IV genes, we hypothesize that mitochondrial complex activity abnormalities may explain phenotypic variation in...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep19544

    authors: Frye RE,Cox D,Slattery J,Tippett M,Kahler S,Granpeesheh D,Damle S,Legido A,Goldenthal MJ

    更新日期:2016-01-29 00:00:00

  • Essential ion binding residues for Na+ flow in stator complex of the Vibrio flagellar motor.

    abstract::The bacterial flagellar motor is a unique supramolecular complex which converts ion flow into rotational force. Many biological devices mainly use two types of ions, proton and sodium ion. This is probably because of the fact that life originated in seawater, which is rich in protons and sodium ions. The polar flagell...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-46038-6

    authors: Onoue Y,Iwaki M,Shinobu A,Nishihara Y,Iwatsuki H,Terashima H,Kitao A,Kandori H,Homma M

    更新日期:2019-08-02 00:00:00

  • The role of the residence-effect on the outcome of intergroup encounters in Verreaux's sifakas.

    abstract::Intergroup competition has an important impact on the survival and fitness of individuals in group-living species. However, factors influencing the probability of winning an encounter are not fully understood. We studied the influence of numerical advantage and location of the encounter on the chances of winning in ei...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep28457

    authors: Koch F,Signer J,Kappeler PM,Fichtel C

    更新日期:2016-06-22 00:00:00

  • Zinc ion rapidly induces toxic, off-pathway amyloid-β oligomers distinct from amyloid-β derived diffusible ligands in Alzheimer's disease.

    abstract::Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the elderly. Zinc (Zn) ion interacts with the pathogenic hallmark, amyloid-β (Aβ), and is enriched in senile plaques in brain of AD patients. To understand Zn-chelated Aβ (ZnAβ) species, here we systematically characterized ZnAβ aggregates by ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-23122-x

    authors: Lee MC,Yu WC,Shih YH,Chen CY,Guo ZH,Huang SJ,Chan JCC,Chen YR

    更新日期:2018-03-19 00:00:00

  • New Insight into Mixing Fluoride and Chloride in Bioactive Silicate Glasses.

    abstract::Adding fluoride into bioactive glasses leads to fluorapatite formation and a decrease in glass transition temperature. Recently, chloride has been introduced into glasses as an alternative to fluoride. The presence of the large chloride ion lowers glass crystallisation tendency and increases glass molar volume, which ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-19544-2

    authors: Chen X,Chen X,Pedone A,Apperley D,Hill RG,Karpukhina N

    更新日期:2018-01-22 00:00:00

  • Targeting extra-oral bitter taste receptors modulates gastrointestinal motility with effects on satiation.

    abstract::Bitter taste receptors (TAS2Rs) are present in extra-oral tissues, including gut endocrine cells. This study explored the presence and mechanism of action of TAS2R agonists on gut smooth muscle in vitro and investigated functional effects of intra-gastric administration of TAS2R agonists on gastric motility and satiat...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep15985

    authors: Avau B,Rotondo A,Thijs T,Andrews CN,Janssen P,Tack J,Depoortere I

    更新日期:2015-11-06 00:00:00

  • Infrared laser pulse triggers increased singlet oxygen production in tumour cells.

    abstract::Photodynamic therapy (PDT) is a technique developed to treat the ever-increasing global incidence of cancer. This technique utilises singlet oxygen ((1)O2) generation via a laser excited photosensitiser (PS) to kill cancer cells. However, prolonged sensitivity to intensive light (6-8 weeks for lung cancer), relatively...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep03484

    authors: Sokolovski SG,Zolotovskaya SA,Goltsov A,Pourreyron C,South AP,Rafailov EU

    更新日期:2013-12-12 00:00:00

  • Vlasouliolides A-D, four rare C17/C15 sesquiterpene lactone dimers with potential anti-inflammatory activity from Vladimiria souliei.

    abstract::Vlasouliolides A-D (1-4), four rare sesquiterpene lactone dimers, were isolated from Vladimiria souliei. The common structural characteristic of 1-4 is the C32 skeleton comprising two sesquiterpene lactone units linked by a C11-C13' single bond with one acetyl connected to the C-13 position of one of the two sesquiter...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep43837

    authors: Chen LP,Wu GZ,Zhang JP,Ye J,Liu QX,Shen YH,Li HL,Zhang WD

    更新日期:2017-03-03 00:00:00

  • Significance of penile hypersensitivity in premature ejaculation.

    abstract::To assess the correlation between penile hypersensitivity and premature ejaculation (PE), a total of 420 consecutive subjects attending our andrologic clinic for suspected PE were enrolled. The entire cohort was asked to complete the self-report intravaginal ejaculation latency time (IELT) by stopwatch. According to t...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-09155-8

    authors: Guo L,Liu Y,Wang X,Yuan M,Yu Y,Zhang X,Zhao S

    更新日期:2017-09-05 00:00:00

  • p53 functional states are associated with distinct aldehyde dehydrogenase transcriptomic signatures.

    abstract::p53 and aldehyde dehydrogenase (ALDH) have been implicated in key tumorigenesis processes including cancer initiating cell (CIC) maintenance; however, the relationship between these two mediators remains poorly defined. In this study, ALDH isoform expression diversity was revealed in CICs with disparate p53 functional...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-57758-5

    authors: Gui S,Xie X,O'Neill WQ,Chatfield-Reed K,Yu JG,Teknos TN,Pan Q

    更新日期:2020-01-23 00:00:00