Abstract：
:One of the potential antibiofilm strategies is to use lytic phages and phage-derived polysaccharide depolymerases. The idea is to uncover bacteria embedded in the biofilm matrix making them accessible and vulnerable to antibacterials and the immune system. Here we present the antibiofilm efficiency of lytic phage KP34 equipped with virion-associated capsule degrading enzyme (depolymerase) and its recombinant depolymerase KP34p57, depolymerase-non-bearing phage KP15, and ciprofloxacin, separately and in combination, using a multidrug-resistant K. pneumoniae biofilm model. The most effective antibiofilm agents were (1) phage KP34 alone or in combination with ciprofloxacin/phage KP15, and (2) depolymerase KP34p57 with phage KP15 and ciprofloxacin. Secondly, applying the commonly used biofilm microtiter assays: (1) colony count, (2) LIVE/DEAD BacLight Bacterial Viability Kit, and (3) crystal violet (CV) biofilm staining, we unravelled the main advantages and limitations of the above methods in antibiofilm testing. The diverse mode of action of selected antimicrobials strongly influenced obtained results, including a false positive enlargement of biofilm mass (CV staining) while applying polysaccharide degrading agents. We suggest that to get a proper picture of antimicrobials' effectiveness, multiple examination methods should be used and the results must be read considering the principle of each technique and the antibacterial mechanism.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Latka A,Drulis-Kawa Zdoi
10.1038/s41598-020-77198-5subject
Has Abstractpub_date
2020-11-23 00:00:00pages
20338issue
1issn
2045-2322pii
10.1038/s41598-020-77198-5journal_volume
10pub_type
杂志文章abstract：:This study describes a new two-step process to cool the thermal vibration of microcantilevers. The process combines active mechanical feedback cooling and optical cavity cooling. A micro-Fabry-Perot interferometer, built in-house, is set atop a microcantilever to measure the vibration amplitude, the high optical power...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-55496-x
更新日期：2019-12-13 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22916-3
更新日期：2018-03-19 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2015-11-19 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41262
更新日期：2017-02-01 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-08-21 00:00:00
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pub_type： 杂志文章
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更新日期：2016-05-13 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18822
更新日期：2016-01-05 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2019-05-23 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
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更新日期：2019-05-02 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31441
更新日期：2016-08-11 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39450
更新日期：2016-12-22 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40731-2
更新日期：2019-05-14 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43029
更新日期：2017-02-22 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50659-2
更新日期：2019-10-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-11-20 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-03-09 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-05-08 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05227
更新日期：2014-06-09 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-03-18 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2015-02-05 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45777
更新日期：2017-04-04 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12079
更新日期：2015-07-15 00:00:00
abstract：:This corrects the article DOI: 10.1038/srep42494. ...
journal_title：Scientific reports
pub_type： 杂志文章,已发布勘误
doi：10.1038/srep46896
更新日期：2017-08-24 00:00:00
