Abstract:
:In an era when immunohistochemistry (IHC) is increasingly depended on for histological subtyping, and IHC-determined biomarker informing rapid treatment choices is on the horizon; reproducible, quantifiable techniques are required. This study aimed to compare automated IHC scoring to quantify 6 DNA damage response protein markers using a tissue microarray of 66 ovarian cancer samples. Accuracy of quantification was compared between manual H-score and computer-aided quantification using Aperio ImageScope with and without a tissue classification algorithm. High levels of interobserver variation was seen with manual scoring. With automated methods, inclusion of the tissue classifier mask resulted in greater accuracy within carcinomatous areas and an overall increase in H-score of a median of 11.5% (0%-18%). Without the classifier, the score was underestimated by a median of 10.5 (5.2-25.6). Automated methods are reliable and superior to manual scoring. Fixed algorithms offer the reproducibility needed for high-throughout clinical applications.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Gentles L,Howarth R,Lee WJ,Sharma-Saha S,Ralte A,Curtin N,Drew Y,O'Donnell RLdoi
10.1136/jclinpath-2020-207081subject
Has Abstractpub_date
2020-11-19 00:00:00eissn
0021-9746issn
1472-4146pii
jclinpath-2020-207081pub_type
杂志文章abstract::Serum glycocholic acid (SGC) was measured by radioimmunoassay in 277 samples from 122 children with hepatobiliary disorders and from 23 healthy age-matched controls. In patients with hepatobiliary disease the SGC was more frequently abnormal (83%) than values for serum albumin (7%), prothrombin time (17%), bilirubin (...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.35.9.1011
更新日期:1982-09-01 00:00:00
abstract:AIM:To determine the reliability of international normalised ratio (INR) measurement in primary care by practice nurses using near patient testing (NPT), in comparison with results obtained within hospital laboratories by varied methods. METHODS:As part of an MRC funded study into primary care oral anticoagulation man...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.52.7.494
更新日期:1999-07-01 00:00:00
abstract::Protein HC and protein HC-IgA complex were measured in 18 different types of fluid sample from healthy subjects and patients with different illnesses to determine if the concentrations of protein HC and protein HC-IgA complexes could be used to monitor certain diseases, when measured separately. The normal values for ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.11.1176
更新日期:1988-11-01 00:00:00
abstract::A case of chronic neutrophilic leukaemia (CNL) is reported. The diagnosis was based on leucocytosis with mature neutrophils, a raised leucocyte alkaline phosphatase score, negative Philadelphia chromosome, and extensive infiltration of neutrophils in various differentiation stages into the bone marrow. When viewed by ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.4.367
更新日期:1994-04-01 00:00:00
abstract:AIMS:To identify the amyloid protein in a patient with amyloidosis localised to the urinary bladder, and to see whether subtyping of the protein by sequence analysis increases the understanding of the selection of the urinary bladder as the site of amyloid deposition. METHODS:A patient with gross haematuria and a cong...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.54.12.920
更新日期:2001-12-01 00:00:00
abstract:AIMS:To investigate whether the use of recombinant early antigens for detection of antibodies to human cytomegalovirus (HCMV) gene products CM(2) (UL44, UL57) and p52 (UL44) is specific in the diagnosis and differentiation of active HCMV infection in a subset of patients with chronic fatigue syndrome (CFS), a diagnosis...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2007.050633
更新日期:2008-05-01 00:00:00
abstract::Serum haptoglobin levels have been studied in patients with a number of haemolytic disorders. In general, serum haptoglobins were low in patients with increased haemolysis. Values were reduced irrespective of whether haemolysis occurred intravascularly or extravascularly. The reduction was related to the amount of hae...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.13.6.478
更新日期:1960-11-01 00:00:00
abstract:BACKGROUND:The disorders associated with metabolic syndrome (MS) can lead to renal disease. IgA nephropathy is the most common form of glomerulonephritis, and many patients with this disorder progress to renal failure. AIMS:To identify the effect of MS on IgA nephropathy by retrospectively comparing patients who had I...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2009.074278
更新日期:2010-08-01 00:00:00
abstract:AIM:To determine whether the pattern of involvement of the gastric mucosa in lymphocytic gastritis is predictive of the presence or absence of duodenal pathology. METHODS:50 cases (M:F, 26:24; median age 57 years) diagnosed as lymphocytic gastritis between 1986 and 1998 with concurrent duodenal (D2) biopsies were iden...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.52.11.815
更新日期:1999-11-01 00:00:00
abstract::A case of acute myelomonocytic leukaemia (AMMOL) is reported in which skin infiltration with xanthomatous nodules was the presenting feature. The histological, including ultrastructural, appearances are described. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.35.11.1200
更新日期:1982-11-01 00:00:00
abstract::Haematological malignancies are a diverse group of cancers that affect the blood, bone marrow and lymphatic systems. Laboratory diagnosis of haematological malignancies is dependent on combining several technologies, including morphology, immunophenotyping, cytogenetics and molecular genetics correlated clinical detai...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2016-204029
更新日期:2017-06-01 00:00:00
abstract::In a case of atypical mucin-producing bronchial carcinoid in a 40-year-old man covert metastases were present at the time of lobectomy and were revealed at necropsy--a feature highlighting the malignant potential of the atypical carcinoid. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.12.1218
更新日期:1978-12-01 00:00:00
abstract:AIMS:Congenital neutropenia (CN) is a rare inherited disease that results in recurrent, life-threatening bacterial infections due to a deficiency of mature neutrophils. They are usually caused by heterozygous ELANE mutations although mutations in other genes like HAX-1, G6PC3 and GFI1 have also been reported. Identifyi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2019-206306
更新日期:2020-06-01 00:00:00
abstract:AIM:Definitive distinction between low-grade astrocytoma and astrogliosis is a long-standing difficulty due to their similar histopathological characteristics. To clarify differences in biological significance, this study focused on various components of the cell cycle machinery and proliferation as key parameters, com...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2007.051961
更新日期:2008-03-01 00:00:00
abstract:AIMS:To ascertain the effect of enhanced surveillance following an outbreak of waterborne cryptosporidiosis on the number of faecal specimens submitted to the local microbiology laboratory and the number positive for common enteric pathogens. The outbreak provided an opportunity to estimate the extent of routine under ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.55.4.271
更新日期:2002-04-01 00:00:00
abstract::Attempts to detect hepatitis B antigen in faeces and bile should take into account the degradation and disappearance of the surface antigens in an environment containing proteolytic enzymes in the presence of bile salts as in the intestinal lumen. The cores of the Dane particles are much more stable than the surface a...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.27.9.693
更新日期:1974-09-01 00:00:00
abstract:AIMS:To evaluate and compare the long term prognostic value of the WHO classification and the computerised multivariate morphometrical D score in endometrial hyperplasia. To test the reproducibility of the D score in two different centres. METHODS:Histopathological WHO classification and computerised morphometrical an...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,多中心研究
doi:10.1136/jcp.53.9.697
更新日期:2000-09-01 00:00:00
abstract::Laboratory studies on clotrimazole showed that it had marked activity in vitro against all the Candida spp. and Cryptococcus spp. tested, against almost all strains of dermatophytes, and against Aspergillus spp and other fungal genera responsible for systemic mycoses; it had limited activity towards Gram-positive bact...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.25.12.1089
更新日期:1972-12-01 00:00:00
abstract::Angiogenesis plays a crucial role in melanoma metastasis and progression. In recent years, numerous studies have investigated the prognostic and clinical significance of this phenomenon, and the development of molecular techniques has enabled us to achieve a better understanding of angiogenesis in melanoma. Herein, we...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2015-203482
更新日期:2016-06-01 00:00:00
abstract::Mast cells were identified by electronmicroscopy in the alveolar wall of the lung in 20 subjects (10 normal, 10 abnormal). A quantitative and qualitative study was made of the mast cells. In the normal lung there was an average concentration of 350 mast cells/mm2 of alveolar wall and in the abnormal 523/mm2. Mast cell...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.12.1333
更新日期:1981-12-01 00:00:00
abstract::This paper urges the benefits of applying more widely a method for pathology laboratory costing originally devised for a clinical chemistry department, and illustrates these with examples drawn from costing studies in three clinical laboratories. Heads of pathology departments, laboratory managers, administrators and ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.9.1028
更新日期:1983-09-01 00:00:00
abstract::Serum IgM, IgA, and IgG concentrations were measured in 19 patients suffering from blood-dependent homozygous beta-thalassaemia. The study was prospective and the same patients served as presplenectomy controls. IgG levels rose rapidly and continuously after splenectomy IgA levels also rose but not so sharply. IgM fel...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.6.546
更新日期:1978-06-01 00:00:00
abstract::Incomplete expression of human haemoglobin beta-chains at birth may lead to difficulty in the early demonstration of an inherited beta-chain variant. In this case, the rare unstable variant, Hb Tacoma beta30 (B12) arg leads to ser, although not present in cord blood in sufficient amounts to be easily detected by routi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.9.883
更新日期:1978-09-01 00:00:00
abstract::Autoinfective strongyloidiasis is often fatal in immunosuppressed patients or in immunocomprised hosts. An interesting case of Strongyloides stercolaris hyperinfection was seen in an immunocompetent patient. This report describes a case of fatal strogyloidiasis in a 79 year old man, who had suffered gastrointestinal d...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2004.017756
更新日期:2005-04-01 00:00:00
abstract::Human epidermal growth factor receptor 2 (HER2) testing is required for newly diagnosed breast cancer and advised for recurrent and metastatic breast cancer, to determine treatment planning using HER2-directed therapy in the neoadjuvant, adjuvant and advanced disease settings. Wide variation, nationally, in the turnar...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2013-201819
更新日期:2014-02-01 00:00:00
abstract::Immunoreactive plasminogen activators were studied in tissue sections using a peroxidase method and monospecific antibodies to tissue plasminogen activator produced by a melanoma. Tissue plasminogen activator reactivity was found in skin melanomas and in endothelial and smooth muscle cells of arteries and veins. Vesse...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.2.140
更新日期:1985-02-01 00:00:00
abstract:BACKGROUND:Epidermal growth factor receptor (EGFR) gene mutation at the kinase domain and EGFR gene amplification are reported to be predictors of the response to EGFR tyrosine kinase inhibitors in lung cancer cases. In malignant mesothelioma (MM), the role of EGFR is less clear. METHODS:Thirty-eight MM specimens were...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2011-200631
更新日期:2012-06-01 00:00:00
abstract:AIM:To examine a model for the evaluation of appropriateness of testing in an emergency biochemistry laboratory. METHODS:A model was devised in which incoming emergency test requests were categorised as appropriate or inappropriate. Explicit criteria were used to define eight minor categories, which were chosen to ref...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.12.1126
更新日期:1995-12-01 00:00:00
abstract:AIMS:To study the overexpression of p53 protein in Barrett's oesophagus with adenocarcinoma, and to correlate this expression with the pathological features of Barrett's syndrome. METHODS:Immunohistochemical staining was performed on frozen sections with a monoclonal antibody directed against wild type and mutated p53...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.4.330
更新日期:1993-04-01 00:00:00
abstract::Muscle biopsy samples were analysed from five control subjects, four patients with mild to moderate fibre atrophy and four patients with severe atrophy. Patchy increase in lipid was noted with oil red O staining but there was no consistent association of lipid with selective fibre types. Ultrastructural studies demons...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.7.778
更新日期:1983-07-01 00:00:00