Abstract：
:Confinement and crowding have been shown to affect protein fates, including folding, functional stability, and their interactions with self and other proteins. Using both theoretical and experimental studies, researchers have established the independent effects of confinement or crowding, but only a few studies have explored their effects in combination; therefore, their combined impact on protein fates is still relatively unknown. Here, we investigated the combined effects of confinement and crowding on protein stability using the pores of agarose hydrogels as a confining agent and the biopolymer, dextran, as a crowding agent. The addition of dextran further stabilized the enzymes encapsulated in agarose; moreover, the observed increases in enhancements (due to the addition of dextran) exceeded the sum of the individual enhancements due to confinement and crowding. These results suggest that even though confinement and crowding may behave differently in how they influence protein fates, these conditions may be combined to provide synergistic benefits for protein stabilization. In summary, our study demonstrated the successful use of polymer-based platforms to advance our understanding of how in vivo like environments impact protein function and structure.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Ross ML,Kunkel J,Long S,Asuri Pdoi
10.3390/ijms21228516subject
Has Abstractpub_date
2020-11-12 00:00:00issue
22issn
1422-0067pii
ijms21228516journal_volume
21pub_type
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更新日期：2017-01-26 00:00:00
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