Abstract：
OBJECTIVES:Dentifrices containing zinc reduce gingival inflammation and bleeding better than control dentifrices (no zinc). How zinc might work is not understood. We have shown that lysine decarboxylase (LdcE), an enzyme from Eikenella corrodens, converts lysine to cadaverine in dental biofilms. The lack of lysine impairs the dentally attached cell barrier to biofilm, causing biofilm products to leak into junctional epithelium and stimulate inflammation. In year-old beagle dogs, immunization with LdcE, induces antibodies that inhibit LdcE activity and retard gingivitis development. We therefore examined whether a zinc-mediated loss of LdcE activity could explain the beneficial effect of zinc dentifrices. METHODS:We grew E. corrodens in modified tryptic soy broth with or without zinc chloride, and extracted LdcE from the cell surface using a Potter Elvehjem homogenizer. RESULTS:Up to 0.96 mM zinc chloride in the bacterial growth medium did not change cell yield, but reduced the extracted protein content by 41% (R2 = 0.27, p < 0.05) and LdcE activity/mg extracted protein by 85% (R2 = 0.90, p < 0.001). In extracts from cells grown without zinc, 78 times this zinc chloride concentration (73 mM) was required to reduce LdcE activity by 75%. CONCLUSIONS:Zinc ions inhibit the production of protein with LdcE activity at E. corrodens cell surfaces. The zinc ions may attach to cysteine residues that are unique to the N-terminal region of LdcE by interfering with the non-covalent polypeptide assembly that produces enzyme activity. CLINICAL SIGNIFICANCE:Zinc ion-mediated inhibition of LdcE assembly may provide a rationale for the improved control of gingival inflammation by zinc dentifrices.
journal_name
J Dentjournal_title
Journal of dentistryauthors
Levine M,Collins LM,Lohinai Zdoi
10.1016/j.jdent.2020.103533subject
Has Abstractpub_date
2020-11-12 00:00:00pages
103533eissn
0300-5712issn
1879-176Xpii
S0300-5712(20)30281-5journal_volume
104pub_type
杂志文章abstract：OBJECTIVE:Cytokine networks regulate innate and adaptive immune responses, which in turn are recognised to direct the progression or arrest of periodontal disease. This study aimed to compare the profile of seven cytokines, implicated in regulating T-cell networks, in gingival crevicular fluid (GCF) samples with differ...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2019.04.009
更新日期：2019-06-01 00:00:00
abstract：OBJECTIVES:To evaluate the ability of a Near Infrared Light Transillumination (NILT) device to detect non-cavitated approximal caries lesions; and to compare its performance to Digital Radiography (DR). METHODS:Thirty human extracted premolars (sound to lesions into the outer one-third of dentin) were selected. Lesion...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2017.05.018
更新日期：2017-08-01 00:00:00
abstract：OBJECTIVES:The objectives of this study were to investigate: (1) the antibacterial activity of two antibacterial monomers, dimethylaminododecyl methacrylate (DMADDM) and dimethylammoniumethyl dimethacrylate (DMAEDM), against eight different species of oral pathogens for the first time; (2) the cytotoxicity of DMAEDM an...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2013.07.006
更新日期：2013-10-01 00:00:00
abstract：OBJECTIVES:The aim of the present study is to investigate variables of the past caries experience as indicators of future caries in a Chinese child population. METHODS:Caries was recorded at baseline and 2 years later in 433, 6-7-year-old Chinese children. Correlation coefficients between variables of the past caries ...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2005.12.010
更新日期：2006-10-01 00:00:00
abstract：OBJECTIVES:A monomer methacryloyloxydodecylpyridinium bromide (MDPB) has antibacterial activity before polymerization. Furthermore, the antibacterial agent is immobilized by the polymerization of MDPB and the resin-based material incorporating MDPB is able to show an antibacterial effect even after being cured. The pur...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/s0300-5712(97)00013-4
更新日期：1998-03-01 00:00:00
abstract：OBJECTIVES:The bonding of acrylic resin to dental prostheses constructed from metal has been the subject of extensive research in recent years, much of it between base-metal alloys such as Ni-Cr alloys and dental composite. The development of techniques for the manufacture of dental appliances from titanium and titaniu...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/s0300-5712(96)00051-6
更新日期：1997-09-01 00:00:00
abstract：BACKGROUND:Untreated dental caries in children remains a public health challenge in poor communities. OBJECTIVES:This prospective controlled clinical trial investigated the effectiveness of a new anti-caries agent, Nano Silver Fluoride (NSF), applied once a year to arrest caries in children. METHODS:One hundred thirt...
journal_title：Journal of dentistry
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jdent.2014.05.017
更新日期：2014-08-01 00:00:00
abstract：:Formaldehyde is responsible for allergic inflammation in acrylic denture wearers and the quantitation of formaldehyde is necessary to study its leaching from denture-base materials. Flow injection analysis was developed to quantify the formaldehyde leached from acrylic resins. Different resins were immersed in aqueous...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/0300-5712(93)90136-e
更新日期：1993-08-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to evaluate the alterations of etched deciduous dentin when submitted to different time and concentrations of sodium hypochlorite (NaOCl), using scanning electron microscopy (SEM). MATERIAL AND METHODS:Forty deciduous anterior teeth were selected, cleaned and ground until expose a f...
journal_title：Journal of dentistry
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jdent.2005.10.003
更新日期：2006-08-01 00:00:00
abstract：OBJECTIVES:To investigate the influence of pre-heating of high-viscosity bulk-fill composite materials on their degree of conversion and shrinkage force formation. METHODS:Four bulk-fill composite materials (Tetric EvoCeram Bulk Fill-TECBF, x-tra fil-XF, QuixFil-QF, SonicFill-SF) and one conventional nano-hybrid resin...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2015.07.014
更新日期：2015-11-01 00:00:00
abstract：OBJECTIVES:To investigate the current prevalence of open access (OA) in the field of dentistry, the means used to provide OA, as well as the association between OA and citation counts. METHODS:PubMed was searched for dental articles published in 2013. The OA status of each article was determined by manually checking G...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2016.02.005
更新日期：2016-04-01 00:00:00
abstract：OBJECTIVES:Some studies have been conducted to evaluate the effect of different topical fluoride regimens on the remineralization of initial carious lesions. This study was conducted to compare the effects of 3 topical fluoride treatments on the surface microhardness, fluoride uptake, and fluorescence lesion area in en...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2009.10.002
更新日期：2010-02-01 00:00:00
abstract：OBJECTIVES:This study investigated whether novel liposome formulations loaded with transforming growth factor β1 (TGF-β1) could promote the odontogenic differentiation of human dental pulp stem cells (hDPSCs) for dentine-pulp regeneration. METHODS:0-100 ng/mL of liposomal TGF-β1 was prepared using the thin-film hydrat...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2020.103501
更新日期：2020-12-01 00:00:00
abstract：INTRODUCTION:Quantification of collagen degradation is an important parameter to evaluate dentin caries progression or the efficacy of caries prevention aid. The aim of this study was to validate the simple light microscopic technique (LM) to evaluate collagen degradation by comparing with hydroxyproline assay techniqu...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2016.01.001
更新日期：2016-04-01 00:00:00
abstract：OBJECTIVES:One of the reasons for using composite inlays is that restorations can be tempered. Composites tempered with heat and light have, at least initially, an enhanced degree of cure which, it is claimed, improves their physical properties, including wear resistance. The aim of this study was to compare the wear r...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/0300-5712(95)00120-4
更新日期：1997-01-01 00:00:00
abstract：OBJECTIVES:All-ceramic crowns have become increasingly used also in the posterior regions, but there are only few studies documenting the clinical outcome of these restorations. The aim was therefore to present the 3-year outcome of zirconia (NobelProcera) single crowns. METHODS:All patients treated with porcelain-ven...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2009.06.002
更新日期：2009-09-01 00:00:00
abstract：OBJECTIVES:The main objective is the characterisation of the setting reaction in glass ionomer cements based on experimental ionomer glasses with different fluorine content and a commercial glass ionomer cement liquid by using 13C CP/MAS-NMR, 29Si, 27Al and 31P MAS-NMR spectroscopy in order to receive information speci...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2005.08.006
更新日期：2006-09-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to evaluate the internal adaptation and marginal properties of ceramic laminate veneers fabricated using pressable and machinable CAD/CAM techniques. MATERIALS AND METHODS:40 ceramic laminate veneers were fabricated by either milling ceramic blocks using a CAD/CAM system (group 1 n...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2012.04.019
更新日期：2012-08-01 00:00:00
abstract：OBJECTIVES:The failure of the bond between acrylic resin denture base material and resin teeth remains a significant problem. This study evaluated the tensile bond strength of specimens produced by commonly employed tooth preparation and processing methods as used in dental laboratories. METHODS:Twenty-two experimenta...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/s0300-5712(98)00034-7
更新日期：1999-02-01 00:00:00
abstract：OBJECTIVE:This study compared the effect of water sorption on the extent of marginal gap reduction in a compomer (Dyract AP, Dentsply) and a composite (Spectrum, Dentsply) over a 12-week storage period. MATERIALS AND METHODS:Artificial gaps were created in 40 borosilicate glass cylinders, each 5mm deep and with an int...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/s0300-5712(01)00053-7
更新日期：2002-01-01 00:00:00
abstract：OBJECTIVES:To validate a new questionnaire for evaluating the 'Oral aesthetic-related quality of life (OARQoL)' of prosthetically restored patients. 'OARQoL' assesses the impact of the self-perceived dental aesthetics on patients' well-being. METHODS:The 'Quality of Life associated with Dental Aesthetic Satisfaction (...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2015.08.010
更新日期：2015-11-01 00:00:00
abstract：OBJECTIVES:To determine the phenotypic and molecular characteristics of Enterococcus faecalis recovered from primary endodontic infections in Brazilian patients. METHODS:Twenty isolates of E. faecalis recovered from 43 Brazilian patients with primary endodontic infections were identified by biochemical profiling (API2...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2013.07.004
更新日期：2013-09-01 00:00:00
abstract：OBJECTIVES:This prospective clinical study compared the performance of implant-retained (study group) and tooth-retained (control) zirconia based fixed dental prostheses (FDPs) with at least 4 units. The null-hypothesis stated that complication rates in both groups are equally distributed. METHODS:The study included p...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2014.10.011
更新日期：2015-01-01 00:00:00
abstract：OBJECTIVES:Long-term survival of two highly viscous glass ionomer cements (Fuji IX GP Fast and Equia Fil) over a period of 6 years in vivo. METHODS:A total of 85 two- or three-surface class II restorations, comprising 43 Equia Fil / Equia Coat and 42 Fuji IX GP Fast / Fuji Coat LC, were placed in 34 patients. The rest...
journal_title：Journal of dentistry
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jdent.2020.103333
更新日期：2020-06-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to investigate the novel mesocellular siliceous foams (MCF) containing CaCO3 nanoparticles (denoted as CMCF) combined with phosphoric acid could occlude dentinal tubules through the formation of biomimetic crystal barrier. METHODS:Ultrastructures of MCF and CMCF were examined by tr...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2019.02.007
更新日期：2019-04-01 00:00:00
abstract：OBJECTIVES:Systemic conditions may affect host susceptibility, disease progression and severity as well as treatment response. Previously, low oestrogen (E(2)) levels were associated with increased bone resorption, due to increased osteoclastogenesis and decreased osteoclast apoptosis. Osteoprotegerin (OPG) is an essen...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2012.01.013
更新日期：2012-05-01 00:00:00
abstract：OBJECTIVES:The in-vitro dissolution of bioactive glass-based toothpastes and their capacity to form apatite-like phases in buffer solutions have been investigated. MATERIALS AND METHODS:The commercial toothpaste samples were tested on immersion in artificial saliva, Earle's salt solution and Tris buffer for duration f...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2017.10.015
更新日期：2018-01-01 00:00:00
abstract：UNLABELLED:Soy beverages are water extracts of whole soybeans and are often promoted as a healthy alternative to bovine milk. Little analysis has been carried out to determine the effects of soy beverages on oral health, especially their potential acidogenicity. OBJECTIVES:The aim of this study was to determine the po...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2012.05.004
更新日期：2012-09-01 00:00:00
abstract：OBJECTIVES:To measure the denaturation temperature (Td) of demineralised dentine matrix as a function of infiltration with water vs. polar solvents vs. adhesive resins. METHODS:Small discs of normal dentine were completely demineralised in 0.5M EDTA. Dried demineralised specimens were placed in water, methanol, ethano...
journal_title：Journal of dentistry
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jdent.2007.10.003
更新日期：2008-01-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to assess the prevalence of direct restorations in posterior teeth in children aged 12, from a birth cohort, and to test the association between the quality of the restorations and individual variables experienced in the life cycle. METHODS:All live-born children in Pelotas, in 200...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2019.103183
更新日期：2019-10-01 00:00:00
