Med23 Regulates Sox9 Expression during Craniofacial Development.

Abstract:

:The etiology and pathogenesis of craniofacial birth defects are multifactorial and include both genetic and environmental factors. Despite the identification of numerous genes associated with congenital craniofacial anomalies, our understanding of their etiology remains incomplete, and many affected individuals have an unknown genetic diagnosis. Here, we show that conditional loss of a Mediator complex subunit protein, Med23 in mouse neural crest cells (Med23 fx/fx ;Wnt1-Cre), results in micrognathia, glossoptosis, and cleft palate, mimicking the phenotype of Pierre Robin sequence. Sox9 messenger RNA and protein levels are both upregulated in neural crest cell-derived mesenchyme surrounding Meckel's cartilage and in the palatal shelves in Med23 fx/fx ;Wnt1-Cre mutant embryos compared to controls. Consistent with these observations, we demonstrate that Med23 binds to the promoter region of Sox9 and represses Sox9 expression in vitro. Interestingly, Sox9 binding to β-catenin is enhanced in Med23 fx/fx ;Wnt1-Cre mutant embryos, which, together with downregulation of Col2a1 and Wnt signaling target genes, results in decreased proliferation and altered jaw skeletal differentiation and cleft palate. Altogether, our data support a cell-autonomous requirement for Med23 in neural crest cells, potentially linking the global transcription machinery through Med23 to the etiology and pathogenesis of craniofacial anomalies such as micrognathia and cleft palate.

journal_name

J Dent Res

authors

Dash S,Bhatt S,Falcon KT,Sandell LL,Trainor PA

doi

10.1177/0022034520969109

subject

Has Abstract

pub_date

2020-11-06 00:00:00

pages

22034520969109

eissn

0022-0345

issn

1544-0591

pub_type

杂志文章
  • The measurement of the effect of toothbrushes on soft tissue abrasion.

    abstract::The effect of toothbrushes on soft tissue abrasion was measured using hamster cheek pouch tissue brushed mechanically for various intervals. The detection of protein removed during brushing was used as an index of tissue abrasion. The method was found to be sensitive in detecting the effects of brush load, number of s...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345770560070301

    authors: Alexander JF,Saffir AJ,Gold W

    更新日期:1977-07-01 00:00:00

  • Effects of positive pressure in odontogenic keratocysts.

    abstract::Intracystic fluid pressure is thought to be involved in odontogenic cyst growth. In this study, we investigated the effects of positive pressure on the expression of interleukin-1alpha (IL-1alpha), matrix metalloproteinases (MMPs), and prostaglandin E2 (PGE2) in odontogenic keratocysts to determine whether this pressu...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910508401008

    authors: Oka S,Kubota Y,Yamashiro T,Ogata S,Ninomiya T,Ito S,Shirasuna K

    更新日期:2005-10-01 00:00:00

  • Calcifiability comparison among selected microorganisms.

    abstract::Seven ATCC strains of bacteria were examined for apatite formation in a chemically-defined calcification-supporting medium and also in a metastable calcium phosphate solution. One, E. coli, calcified in both. One, S. aureus, calcified in the solution, but not in the medium. The other five did not calcify in either. Th...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345810600101001

    authors: Ennever J,Streckfuss JL,Goldschmidt MC

    更新日期:1981-10-01 00:00:00

  • Effect of local vs. systemic bisphosphonate delivery on dental implant fixation in a model of osteonecrosis of the jaw.

    abstract::Locally applied bisphosphonates may improve the fixation of metal implants in bone. However, systemic bisphosphonate treatment is associated with a risk of osteonecrosis of the jaw (ONJ). We hypothesized that local delivery of bisphosphonate from the implant surface improves the fixation of dental implants without com...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034512472335

    authors: Abtahi J,Agholme F,Sandberg O,Aspenberg P

    更新日期:2013-03-01 00:00:00

  • Adherent interactions which may affect microbial ecology in the mouth.

    abstract::Bacteria attach to teeth and oral mucosal surfaces in a surprisingly selective manner, and attachment appears to be the first step in the colonization process. Attachment is thought to involve lectin-like and/or hydrophobic ligands, called adhesins, often present in bacterial surface appendages which interact with rec...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345840630030401

    authors: Gibbons RJ

    更新日期:1984-03-01 00:00:00

  • microRNA-21 Contributes to Orthodontic Tooth Movement.

    abstract::microRNAs could be mechanosensitive and emerge as critical posttranscriptional regulators in the bone-remodeling process. During orthodontic tooth movement (OTM), the application of mechanical force induces alveolar bone remodeling, but whether microRNAs respond to orthodontic force and contribute to OTM is unknown. m...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034516657043

    authors: Chen N,Sui BD,Hu CH,Cao J,Zheng CX,Hou R,Yang ZK,Zhao P,Chen Q,Yang QJ,Jin Y,Jin F

    更新日期:2016-11-01 00:00:00

  • Enamel defects in the deciduous dentition as a potential predictor of defects in the permanent dentition of 8- and 9-year-old children in fluoridated Cheshire, England.

    abstract::It is claimed that dental fluorosis in both deciduous and permanent teeth is increasing in fluoridated and non-fluoridated communities. What is unclear is whether fluoride-induced enamel opacities in the deciduous dentition are associated with the subsequent appearance of enamel defects in the permanent dentition. The...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345960750040301

    authors: Milsom KM,Woodward M,Haran D,Lennon MA

    更新日期:1996-04-01 00:00:00

  • Synergistic, growth-inhibitory effects of chlorhexidine and copper combinations on Streptococcus mutans, Actinomyces viscosus, and Actinomyces naeslundii.

    abstract::The purpose of this investigation was to determine whether chlorhexidine combined with copper would exert a synergistic, growth-inhibitory effect against selected oral bacteria. Actinomyces viscosus, Actinomyces naeslundii, and Streptococcus mutans were all susceptible to chlorhexidine individually, with S. mutans dis...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345930720020901

    authors: Drake DR,Grigsby W,Cardenzana A,Dunkerson D

    更新日期:1993-02-01 00:00:00

  • The reduction in fatigue crack growth resistance of dentin with depth.

    abstract::The fatigue crack growth resistance of dentin was characterized as a function of depth from the dentino-enamel junction. Compact tension (CT) specimens were prepared from the crowns of third molars in the deep, middle, and peripheral dentin. The microstructure was quantified in terms of the average tubule dimensions a...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034511408429

    authors: Ivancik J,Neerchal NK,Romberg E,Arola D

    更新日期:2011-08-01 00:00:00

  • Studies on hypoxia: XII. Detrimental effects of synthetic polyribonucleotides on epiphyseal plates of rats exposed to hypoxia.

    abstract::The effect of different doses of polyadenylic and polyuridylic acids (poly A:U) was studied in control rats and in rats exposed to hypoxia. In the control rats, administration of different doses of poly A:U did not change the thickness of the epiphyseal plate or increase the incorporation of 3H-phenylalanine as judged...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345760550041701

    authors: Koo CS,Kim MK,Han SS

    更新日期:1976-07-01 00:00:00

  • Determinants of requests for water fluoride assay among North Carolina dentists.

    abstract::Fluoride content of drinking water is an important criterion in prescribing fluoride supplements. The majority of practicing dentists and physicians prescribe fluoride supplements, yet many apparently are unaware of the fluoride levels in their patients' drinking water. Characteristics of North Carolina (NC) dentists,...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345860650011301

    authors: Levy SM,Rozier RG,Bawden JW

    更新日期:1986-01-01 00:00:00

  • Trigeminal nociceptors express TLR-4 and CD14: a mechanism for pain due to infection.

    abstract::Although certain bacterial species appear to be risk factors for pain due to odontogenic infections, comparatively little is known about the potential mechanisms mediating this effect. In this study, we tested the hypothesis that trigeminal nociceptive neurons express the TLR4 or CD14 receptors, thus enabling sensory ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910608500108

    authors: Wadachi R,Hargreaves KM

    更新日期:2006-01-01 00:00:00

  • Clinical studies of dental cements: V. Recall evaluation and a zinc phosphate cement.

    abstract::Patients with restorations cemented with a reinforced zinc oxide-eugenol cement and a zinc phosphate cement were recalled after a four- to seven-year interval. No significant differences in clinical success were observed between restoration luted with the two cements. No significant difference in the incidence of cari...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345760550022201

    authors: Silvey RG,Myers GE

    更新日期:1976-03-01 00:00:00

  • The Evolution of Dental Materials over the Past Century: Silver and Gold to Tooth Color and Beyond.

    abstract::The field of dental materials has undergone more of a revolution than an evolution over the past 100 y. The development of new products, especially in the past half century, has occurred at a staggering pace, and their introduction to the market has been equally impressive. The movement has mostly come in the area of ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034518822808

    authors: Bayne SC,Ferracane JL,Marshall GW,Marshall SJ,van Noort R

    更新日期:2019-03-01 00:00:00

  • Analysis of vitamin D and Fcgamma receptor polymorphisms in Japanese patients with generalized early-onset periodontitis.

    abstract::Early-onset periodontitis (EOP) is considered to have a genetic basis which has not been clearly defined. Genetic polymorphisms of the vitamin D receptor (VDR-B-b) and the immunoglobulin-Fc-gamma receptor IIIb (FcgammaRIIIb-NA1-NA2) are associated with bone metabolism and infectious diseases, respectively. The purpose...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345010800120501

    authors: Yoshihara A,Sugita N,Yamamoto K,Kobayashi T,Miyazaki H,Yoshi H

    更新日期:2001-12-01 00:00:00

  • Degradation, fatigue, and failure of resin dental composite materials.

    abstract::The intent of this article is to review the numerous factors that affect the mechanical properties of particle- or fiber-filler-containing indirect dental resin composite materials. The focus will be on the effects of degradation due to aging in different media, mainly water and water and ethanol, cyclic loading, and ...

    journal_title:Journal of dental research

    pub_type: 杂志文章,评审

    doi:10.1177/154405910808700802

    authors: Drummond JL

    更新日期:2008-08-01 00:00:00

  • Resilience of the Oral Microbiota in Health: Mechanisms That Prevent Dysbiosis.

    abstract::Dental diseases are now viewed as a consequence of a deleterious shift in the balance of the normally stable resident oral microbiome. It is known that frequent carbohydrate consumption or reduced saliva flow can lead to caries, and excessive plaque accumulation increases the risk of periodontal diseases. However, whe...

    journal_title:Journal of dental research

    pub_type: 杂志文章,评审

    doi:10.1177/0022034517742139

    authors: Rosier BT,Marsh PD,Mira A

    更新日期:2018-04-01 00:00:00

  • Biological mechanisms of fluorosis and level and timing of systemic exposure to fluoride with respect to fluorosis.

    abstract::Enamel fluorosis can occur following either an acute or chronic exposure to fluoride during tooth formation. Fluorosed enamel is characterized by a retention of amelogenins in the early-maturation stage, and by the formation of a more porous enamel with a subsurface hypomineralization. The mechanisms by which fluoride...

    journal_title:Journal of dental research

    pub_type: 杂志文章,评审

    doi:10.1177/00220345920710051701

    authors: DenBesten PK,Thariani H

    更新日期:1992-05-01 00:00:00

  • Effect of splint placement on black-pigmented Bacteroides and spirochetes in the dental plaque of beagle dogs.

    abstract::The gingival microbiota in beagle dogs was investigated during a period of 42 days after the placement of continuous wire and arch bar splints. The splints were found to induce an increase in helicoidal-shaped micro-organisms and black-pigmented Bacteroides. Two different asaccharolytic Bacteroides types were isolated...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345840630110601

    authors: Mikx FH,Ngassapa DN,Reijntjens FM,Maltha JC

    更新日期:1984-11-01 00:00:00

  • Kinetics of bone-resorbing activity in developing periapical lesions.

    abstract::The presence and kinetics of bone-resorbing activity in periapical lesions were studied with a rat model system. Lesions were found to expand most rapidly between induction on day 0 and day 15 ("active phase"), with enlargement occurring at a slower rate thereafter (days 20 and 30, "chronic phase"), as assessed by mea...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345910700100901

    authors: Wang CY,Stashenko P

    更新日期:1991-10-01 00:00:00

  • The tensile and shear strength of a base metal weld joint used in dentistry.

    abstract::Samples of a base mental alloy were cast and welded together using a conventional welding procedure. The joints were loaded and placed either in a corrosive environment or in air for varying time periods. The tensile and shear strength of the joints was unaffected by the corrosive environment, and the joints, despite ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345810600021101

    authors: MacEntee MI,Hawbolt EB,Zahel JI

    更新日期:1981-02-01 00:00:00

  • Chondroitin sulfate in palatal wound healing.

    abstract::Chondroitin sulfate is up-regulated in granulation tissue during wound healing. To investigate the role of chondroitin sulfate in the wound-healing process after surgical repair of cleft palate, we isolated and cultured rabbit palatal fibroblasts. Treatment with chondroitin-6-sulfate resulted in a dose-dependent incre...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910408301111

    authors: Zou XH,Foong WC,Cao T,Bay BH,Ouyang HW,Yip GW

    更新日期:2004-11-01 00:00:00

  • Massively Increased Caries Susceptibility in an Irf6 Cleft Lip/Palate Model.

    abstract::Patients with cleft lip/palate (CLP) have been reported, in some studies, to exhibit an increased prevalence of caries, although the underlying cause for this increase is unknown. In genetically defined mouse models, studies of postnatal sequelae associated with CLP have been hampered by neonatal lethality. Using a co...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034516679376

    authors: Tamasas B,Cox TC

    更新日期:2017-03-01 00:00:00

  • SHED differentiate into functional odontoblasts and endothelium.

    abstract::Studies on mechanisms underlying the differentiation of dental pulp stem cells are critical for the understanding of the biology of odontogenesis and for dental tissue engineering. Here, we tested the hypothesis that stem cells from exfoliated deciduous teeth (SHED) differentiate into functional odontoblasts and endot...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034510368647

    authors: Sakai VT,Zhang Z,Dong Z,Neiva KG,Machado MA,Shi S,Santos CF,Nör JE

    更新日期:2010-08-01 00:00:00

  • VIP Inhibits Porphyromonas gingivalis LPS-induced immune responses in human monocytes.

    abstract::Lipopolysaccharide (LPS) from the Gram-negative pathogen Porphyromonas gingivalis (Pg) stimulates cytokine secretion in immune cells, and thereby initiates the inflammation associated with periodontitis. Modulation of pro-inflammatory cytokine activity is a plausible therapeutic target in periodontal disease. Vasoacti...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910508401106

    authors: Foster N,Cheetham J,Taylor JJ,Preshaw PM

    更新日期:2005-11-01 00:00:00

  • The effects of etching, micro-abrasion, and bleaching on surface enamel.

    abstract::Conservative techniques for improving the appearance of discolored teeth have become popular in the past decade. These include: in-office bleaching with 30% hydrogen peroxide, which is applied on etched enamel with a gauze pad and then exposed to a bleaching light; home bleaching with a mild form of peroxide, such as ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345930720011001

    authors: Tong LS,Pang MK,Mok NY,King NM,Wei SH

    更新日期:1993-01-01 00:00:00

  • Influence of an experimental sodium fluoride dentifrice on dental caries incidence in children.

    abstract::Schoolchildren residing in a fluoride-deficient area were provided either a sodium fluoride (0.1% F) or a nonfluoride dentifrice for home use. About half the children provided each type of dentifrice were also given an initial prophylaxis. Combining the data with regard to the type of dentifrice indicated that the sod...

    journal_title:Journal of dental research

    pub_type: 临床试验,杂志文章

    doi:10.1177/00220345750540013601

    authors: Stookey GK,Beiswanger BB

    更新日期:1975-01-01 00:00:00

  • Impact of Obesity on Orthodontic Tooth Movement in Adolescents: A Prospective Clinical Cohort Study.

    abstract::Obesity is a widespread chronic inflammatory disorder characterized by an increased overall disease burden and significant association with periodontitis. The aim of this prospective clinical cohort study was to investigate the effect of obesity on orthodontic tooth movement. Fifty-five adolescent patients (27 males, ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034516688448

    authors: Saloom HF,Papageorgiou SN,Carpenter GH,Cobourne MT

    更新日期:2017-05-01 00:00:00

  • Relationships between masticatory muscle cross-section and skull shape.

    abstract::Cross-sectional areas of the masseter, temporalis and medial, and lateral pterygoid muscles were determined in 16 subjects by means of computer tomography. In each subject three scans were made, intersecting the thickest part of the muscles at right angles to the fiber direction. The masseter and medial pterygoid musc...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345840630091201

    authors: Weijs WA,Hillen B

    更新日期:1984-09-01 00:00:00

  • Anticariogenic effects of tea in rats.

    abstract::Teas varying in fluoride and tannin concentration were evaluated in rats for anticariogenic activity. There was a direct correlation between fluoride in tea and the inhibition of sulcal caries in rats, whereas no relationship was observed between tannin and this type of lesion. Teas also had a significant effect on ca...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345840630051001

    authors: Rosen S,Elvin-Lewis M,Beck FM,Beck EX

    更新日期:1984-05-01 00:00:00