Abstract:
:Human group-specific component protein (Gc protein) is a multifunctional serum protein which has three common allelic variants, Gc1F, Gc1S and Gc2 in humans. Gc1 contains an O-linked trisaccharide [sialic acid-galactose-N-acetylgalactosamine (GalNAc)] on the threonine420 (Thr420) residue and can be converted to a potent macrophage activating factor (GcMAF) by selective removal of sialic acid and galactose, leaving GalNAc at Thr420. In contrast, Gc2 is not glycosylated. GcMAF is considered a promising candidate for immunotherapy and antiangiogenic therapy of cancers and has attracted great interest, but it remains difficult to compare findings among research groups because different procedures have been used to prepare GcMAF. Here, we present a simple, practical method to prepare high-quality GcMAF by overexpressing Gc-protein in a serum-free suspension culture of ExpiCHO-S cells, without the need for a de-glycosylation step. We believe this protocol is suitable for large-scale production of GcMAF for functional analysis and clinical testing.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Nabeshima Y,Abe C,Kawauchi T,Hiroi T,Uto Y,Nabeshima YIdoi
10.1038/s41598-020-75571-ysubject
Has Abstractpub_date
2020-11-05 00:00:00pages
19122issue
1issn
2045-2322pii
10.1038/s41598-020-75571-yjournal_volume
10pub_type
杂志文章abstract::The autoimmune disorder Aicardi-Goutières syndrome (AGS) is characterized by a constitutive type I interferon response. SAMHD1 possesses both dNTPase and RNase activities and mutations in SAMHD1 cause AGS; however, how SAMHD1-deficiency causes the type I interferon response in patients with AGS remains unknown. Here, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-18308-8
更新日期:2018-01-08 00:00:00
abstract::Human induced pluripotent stem cells (hiPSCs) represent an almost limitless source of cells for disease modelling and drug screening applications. Here we established an efficient and robust 3D platform for cardiomyocyte (CMs) production from hiPSCs, solely through small-molecule-based temporal modulation of the Wnt s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45047-9
更新日期:2019-06-25 00:00:00
abstract::In response to infestation by herbivores, rice plants rapidly biosynthesize defense compounds by activating a series of defense-related pathways. However, which defensive compounds in rice are effective against herbivores remains largely unknown. We found that the infestation of white-backed planthopper (WBPH) Sogatel...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62752-y
更新日期:2020-04-01 00:00:00
abstract::Circadian clocks are endogenous oscillators driving daily rhythms in physiology. The cell-autonomous clock is governed by an interlocked network of transcriptional feedback loops. Hundreds of clock-controlled genes (CCGs) regulate tissue specific functions. Transcriptome studies reveal that different organs (e.g. live...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05782
更新日期:2014-07-22 00:00:00
abstract::While molecular-targeted drugs have demonstrated strong therapeutic efficacy against diverse diseases such as cancer and infection, the appearance of drug resistance associated with genetic variations in individual patients or pathogens has severely limited their clinical efficacy. Therefore, precision medicine approa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58877-9
更新日期:2020-02-07 00:00:00
abstract::Dinosaurs thrived and reproduced in various regions worldwide, including the Arctic. In order to understand their nesting in diverse or extreme environments, the relationships between nests, nesting environments, and incubation methods in extant archosaurs were investigated. Statistical analyses reveal that species of...
journal_title:Scientific reports
pub_type: 历史文章,杂志文章
doi:10.1038/s41598-018-21386-x
更新日期:2018-03-15 00:00:00
abstract::Machine learning algorithms have recently shown their precision and potential in many different use cases and fields of medicine. Most of the algorithms used are supervised and need a large quantity of labeled data to achieve high accuracy. Also, most applications of machine learning in medicine are attempts to mimic ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73902-7
更新日期:2020-10-12 00:00:00
abstract::Nowadays, one of the main challenges is moving towards an eco-sustainable agriculture, able to preserve the food production through a reduced use of pesticides. Current global food sustenance by intensive agriculture is mainly based on economic crop monocultures and drastically reduces the biodiversity, increasing the...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76247-3
更新日期:2020-11-05 00:00:00
abstract::Steep-slope transistors allow to scale down the supply voltage and the energy per computed bit of information as compared to conventional field-effect transistors (FETs), due to their sub-60 mV/decade subthreshold swing at room temperature. Currently pursued approaches to achieve such a subthermionic subthreshold swin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00359-6
更新日期:2017-03-23 00:00:00
abstract::Eph receptors have emerged as targets for therapy in both neoplastic and non-neoplastic disease, however, particularly in non-neoplastic diseases, redundancy of function limits the effectiveness of targeting individual Eph proteins. We have shown previously that a soluble fusion protein, where the EphA4 ectodomain was...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06685-z
更新日期:2017-07-26 00:00:00
abstract::The discovery of circulating tumour DNA molecules created a paradigm shift in tumour biomarkers as predictors of recurrence. Non-invasive prenatal testing (NIPT) to detect circulating cell-free foetal DNA in maternal plasma is increasingly recognised as a valuable substitute to perceive foetal copy number variation (C...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29381-y
更新日期:2018-07-25 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-65968-0
更新日期:2020-06-04 00:00:00
abstract::Cooperative behaviors are common in humans, and they are the fundamental basis of our society. Theoretical and experimental studies have modeled environments where the behaviors of humans, or agents, have been restricted to analyze their social behavior. However, it is important that such studies can be generalized to...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12481
更新日期:2015-07-28 00:00:00
abstract::Ca2+ release-activated Ca2+ (CRAC) channels elevate cytoplasmic Ca2+ concentration, which is essential for T cell activation, differentiation and effector functions. T cell receptor stimulation induces depletion of the endoplasmic reticulum (ER) Ca2+ stores, which is sensed by stromal interaction molecule 1 (STIM1). S...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71489-7
更新日期:2020-09-02 00:00:00
abstract::Autosomal dominant North Carolina macular dystrophy (NCMD) is believed to represent a failure of macular development. The disorder has been linked to two loci, MCDR1 (chromosome 6q16) and MCDR3 (chromosome 5p15-p13). Recently, non-coding variants upstream of PRDM13 (MCDR1) and a duplication including IRX1 (MCDR3) have...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06387-6
更新日期:2017-08-08 00:00:00
abstract::Bioactive gibberellins (GAs) are essential phytohormones involved in the regulation of many aspects of plant development. GA receptors are crucial in GA signal transduction in plants. The GA receptor GoGID1 promotes plant elongation and improves biomass production when ectopically expressed in tobacco. Here, we discov...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41296
更新日期:2017-01-27 00:00:00
abstract::A major challenge to routine non-invasive, nanoscale magnetic imaging is the development of Hall sensors that are stable under ambient conditions and retain low minimum detectable fields down to nanoscale dimensions. To address these issues we have fabricated and characterised chemical vapour deposition (CVD) graphene...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50823-8
更新日期:2019-10-08 00:00:00
abstract::The most important goals of brain network analyses are to (a) detect pivotal regions and connections that contribute to disproportionate communication flow, (b) integrate global information, and (c) increase the brain network efficiency. Most centrality measures assume that information propagates in networks with the ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42322-7
更新日期:2019-04-11 00:00:00
abstract::Given that many fundamental questions in neuroscience are still open, it seems pertinent to explore whether the brain might use other physical modalities than the ones that have been discovered so far. In particular it is well established that neurons can emit photons, which prompts the question whether these biophoto...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36508
更新日期:2016-11-07 00:00:00
abstract::Methamphetamine (METH) is a highly addictive psychostimulant that causes long-lasting effects in the brain and increases the risk of developing neurodegenerative diseases. The cellular and molecular effects of METH in the brain are functionally linked to alterations in glutamate levels. Despite the well-documented eff...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80917-7
更新日期:2021-01-14 00:00:00
abstract::Climatic changes can induce geographic expansion and altitudinal shifts in the distribution of invasive species by offering more thermally suitable habitats. At the remote sub-Antarctic Kerguelen Islands, the predatory insect Merizodus soledadinus (Coleoptera: Carabidae), introduced in 1913, rapidly invaded coastal ha...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-57868-0
更新日期:2020-01-27 00:00:00
abstract::Owing to its simplicity and low temperature conditions, magnesiothermic reduction of silica is one of the most powerful methods for producing silicon nanostructures. However, incomplete reduction takes place in this process leaving unconverted silica under the silicon layer. This phenomenon limits the use of this meth...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09014
更新日期:2015-03-11 00:00:00
abstract::Alzheimer's disease (AD) is a severe neurodegenerative disorder for which identification of differentially expressed genes is one way to find new therapeutic targets. Here, we conducted analysis to identify age-independent, AD-specific genes. We found that the MET, WIF1, and NPTX2 genes are downregulated in AD. WIF1 a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21256-6
更新日期:2018-02-09 00:00:00
abstract::We propose a novel method to determine the complex amplitude of each eigenfunction composing a vibrational wavepacket of / molecular ions evolving with a ~10 fs time scale. We find that the two-dimensional spectrogram of the kinetic energy release (KER) of H(+)/D(+) fragments plotted against the time delay of the prob...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11366
更新日期:2015-06-12 00:00:00
abstract::Understanding interactions between cell-penetrating peptides and biomembrane under tension can help improve drug delivery and elucidate mechanisms underlying fundamental cellular events. As far as the effect of membrane tension on translocation, it is generally thought that tension should disorder the membrane structu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12808
更新日期:2015-08-03 00:00:00
abstract::MicroRNA is responsible for the fine-tuning of fundamental cellular activities and human disease development. The altered availability of microRNAs, target mRNAs, and other types of endogenous RNAs competing for microRNA interactions reflects the dynamic and conditional property of microRNA-mediated gene regulation th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13470-5
更新日期:2017-10-17 00:00:00
abstract::The geometry of metal nanoparticles greatly affects the properties of the localized surface plasmon resonance and surface-enhanced Raman scattering. The synthesis of metal nanoparticles with controllable geometry has thus attracted extensive attentions. In this work, we report a modified polyol synthesis approach of s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep10772
更新日期:2015-06-09 00:00:00
abstract:ABSTARCT:Shape memory alloys (SMAs) have the ability to show large recoverable shape changes upon temperature, stress or magnetic field cycling. Their shape memory, material and magnetic properties (e.g. transformation temperatures, strain, saturation magnetization and strength) determine their prospects for applicatio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13434-9
更新日期:2017-11-01 00:00:00
abstract::Breast cancer is a heterogeneous disease with differences in its clinical, molecular and biological features. Traditionally, immunohistochemical markers together with clinicopathologic parameters are used to classify breast cancer and to predict disease outcome. Triple-negative breast cancer (TNBC) is a particular typ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44414
更新日期:2017-03-15 00:00:00
abstract::Malaria in India, while decreasing, remains a serious public health problem, and the contribution of submicroscopic and asymptomatic infections to its persistence is poorly understood. We conducted community surveys and clinic studies at three sites in India differing in their eco-epidemiologies: Chennai (Tamil Nadu),...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53386-w
更新日期:2019-11-19 00:00:00