Transformation of nonencapsulated Streptococcus pneumoniae during systemic infection.

Abstract:

:Streptococcus pneumoniae (pneumococcus) is a principal cause of bacterial middle ear infections, pneumonia, and meningitis. Capsule-targeted pneumococcal vaccines have likely contributed to increased carriage of nonencapsulated S. pneumoniae (NESp). Some NESp lineages are associated with highly efficient DNA uptake and transformation frequencies. However, NESp strains lack capsule that may increase disease severity. We tested the hypothesis that NESp could acquire capsule during systemic infection and transform into more virulent pneumococci. We reveal that NESp strains MNZ67 and MNZ41 are highly transformable and resistant to multiple antibiotics. Natural transformation of NESp when co-administered with heat-killed encapsulated strain WU2 in a murine model of systemic infection resulted in encapsulation of NESp and increased virulence during bacteremia. Functional capsule production increased the pathogenic potential of MNZ67 by significantly decreasing complement deposition on the bacterial surface. However, capsule acquisition did not further decrease complement deposition on the relatively highly pathogenic strain MNZ41. Whole genome sequencing of select transformants demonstrated that recombination of up to 56.7 kbp length occurred at the capsule locus, along with additional recombination occurring at distal sites harboring virulence-associated genes. These findings indicate NESp can compensate for lack of capsule production and rapidly evolve into more virulent strains.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Bradshaw JL,Rafiqullah IM,Robinson DA,McDaniel LS

doi

10.1038/s41598-020-75988-5

subject

Has Abstract

pub_date

2020-11-03 00:00:00

pages

18932

issue

1

issn

2045-2322

pii

10.1038/s41598-020-75988-5

journal_volume

10

pub_type

杂志文章
  • Contrasting seasonal patterns of telomere dynamics in response to environmental conditions in the ectothermic sand lizard, Lacerta agilis.

    abstract::Telomeres, the protective, terminal parts of the chromosomes erode during cell division and as a result of oxidative damage by reactive oxygen species (ROS). Ectotherms rely on the ambient temperature for maintaining temperature-dependent metabolic rate, regulated through behavioural thermoregulation. Their temperatur...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-57084-5

    authors: Axelsson J,Wapstra E,Miller E,Rollings N,Olsson M

    更新日期:2020-01-13 00:00:00

  • New molecular insights into the tyrosyl-tRNA synthase inhibitors: CoMFA, CoMSIA analyses and molecular docking studies.

    abstract::Drug resistance caused by excessive and indiscriminate antibiotic usage has become a serious public health problem. The need of finding new antibacterial drugs is more urgent than ever before. Tyrosyl-tRNA synthase was proved to be a potent target in combating drug-resistant bacteria. In silico methodologies including...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-10618-1

    authors: Li S,Fan J,Peng C,Chang Y,Guo L,Hou J,Huang M,Wu B,Zheng J,Lin L,Xiao G,Chen W,Liao G,Guo J,Sun P

    更新日期:2017-09-14 00:00:00

  • Lighting up ultraviolet fluorescence from chicken albumen through plasmon resonance energy transfer of gold nanoparticles.

    abstract::Au nanoparticles (AuNPs), which easily aggregate in organic thin film, are observed to well-disperse in chicken albumen thin films. The incorporated AuNPs is distributed uniformly inside the thin film and is as dense as 650 (particles/μm(3)). In addition, enhanced ultraviolet (UV) fluorescence centered at 350 nm is ob...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep01505

    authors: Tsung Chen I,Chang PH,Chang YC,Guo TF

    更新日期:2013-01-01 00:00:00

  • Early-onset obesity dysregulates pulmonary adipocytokine/insulin signaling and induces asthma-like disease in mice.

    abstract::Childhood obesity is a risk factor for asthma, but the molecular mechanisms linking both remain elusive. Since obesity leads to chronic low-grade inflammation and affects metabolic signaling we hypothesized that postnatal hyperalimentation (pHA) induced by maternal high-fat-diet during lactation leads to early-onset o...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep24168

    authors: Dinger K,Kasper P,Hucklenbruch-Rother E,Vohlen C,Jobst E,Janoschek R,Bae-Gartz I,van Koningsbruggen-Rietschel S,Plank C,Dötsch J,Alejandre Alcázar MA

    更新日期:2016-04-18 00:00:00

  • PANDA: Protein function prediction using domain architecture and affinity propagation.

    abstract::We developed PANDA (Propagation of Affinity and Domain Architecture) to predict protein functions in the format of Gene Ontology (GO) terms. PANDA at first executes profile-profile alignment algorithm to search against PfamA, KOG, COG, and SwissProt databases, and then launches PSI-BLAST against UniProt for homologue ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-21849-1

    authors: Wang Z,Zhao C,Wang Y,Sun Z,Wang N

    更新日期:2018-02-22 00:00:00

  • Dual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis.

    abstract::The success of mesenchymal stem cell transplantation is highly dependent on their survival and controlled fate regulation. This study demonstrates that dual-delivery of connective tissue growth factor (CTGF) and fibroblast growth factor 2 (FGF-2) from a core-shell fiber of Silk Fibroin/poly(L-lactic acid-co-ε-caprolac...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-08226-0

    authors: Xu R,Zhao H,Muhammad H,Dong M,Besenbacher F,Chen M

    更新日期:2017-08-17 00:00:00

  • Opportunistic brood theft in the context of colony relocation in an Indian queenless ant.

    abstract::Brood is a very valuable part of an ant colony and behaviours increasing its number with minimum investment is expected to be favoured by natural selection. Brood theft has been well documented in ants belonging to the subfamilies Myrmicinae and Formicinae. In this study we report opportunistic brood theft in the cont...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep36166

    authors: Paul B,Paul M,Annagiri S

    更新日期:2016-10-31 00:00:00

  • Confinement Effect on Porosity and Permeability of Shales.

    abstract::Porosity and permeability are the key factors in assessing the hydrocarbon productivity of unconventional (shale) reservoirs, which are complex in nature due to their heterogeneous mineralogy and poorly connected nano- and micro-pore systems. Experimental efforts to measure these petrophysical properties posse many li...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-56885-y

    authors: Goral J,Panja P,Deo M,Andrew M,Linden S,Schwarz JO,Wiegmann A

    更新日期:2020-01-08 00:00:00

  • Multifunctional rhodamine B appended ROMP derived fluorescent probe detects Al3+ and selectively labels lysosomes in live cells.

    abstract::There a few reports of rhodamine-based fluorescent sensors for selective detection of only Al3+, due to the challenge of identifying a suitable ligand for binding Al3+ ion. The use of fluorophore moieties appended to a polymer backbone for sensing applications is far from mature. Here, we report a new fluorescent prob...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-76525-0

    authors: Gandra UR,Courjaret R,Machaca K,Al-Hashimi M,Bazzi HS

    更新日期:2020-11-11 00:00:00

  • Spatio-temporal processes drive fine-scale genetic structure in an otherwise panmictic seabird population.

    abstract::When and where animals breed can shape the genetic structure and diversity of animal populations. The importance of drivers of genetic diversity is amplified in island populations that tend to have more delineated gene pools compared to continental populations. Studies of relatedness as a function of the spatial distr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-77517-w

    authors: Garrett LJH,Myatt JP,Sadler JP,Dawson DA,Hipperson H,Colbourne JK,Dickey RC,Weber SB,Reynolds SJ

    更新日期:2020-11-26 00:00:00

  • Enhanced absorption in all-dielectric metasurfaces due to magnetic dipole excitation.

    abstract::All-dielectric nanophotonics lies at a forefront of nanoscience and technology as it allows to control light at the nanoscale using its electric and magnetic components. Bulk silicon does not experience any magnetic response, nevertheless, we demonstrate that the metasurface made of silicon parallelepipeds allows to e...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-40226-0

    authors: Terekhov PD,Baryshnikova KV,Greenberg Y,Fu YH,Evlyukhin AB,Shalin AS,Karabchevsky A

    更新日期:2019-03-05 00:00:00

  • Comparison of multi echo T2 relaxation and steady state approaches for myelin imaging in the central nervous system.

    abstract::The traditional approach for measuring myelin-associated water with quantitative magnetic resonance imaging (MRI) uses multi-echo T2 relaxation data to calculate the myelin water fraction (MWF). A fundamentally different approach, abbreviated "mcDESPOT", uses a more efficient steady-state acquisition to generate an eq...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-80585-7

    authors: Dvorak AV,Ljungberg E,Vavasour IM,Lee LE,Abel S,Li DKB,Traboulsee A,MacKay AL,Kolind SH

    更新日期:2021-01-14 00:00:00

  • Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean.

    abstract::Thaumarchaeota and the gene encoding for a subunit of ammonia monooxygenase (amoA) are ubiquitous in Polar Seas, and some Thaumarchaeota also have a gene coding for ureC, diagnostic for urease. Using quantitative PCR we investigated the occurrence of genes and transcripts of ureC and amoA in Arctic samples from winter...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep04661

    authors: Pedneault E,Galand PE,Potvin M,Tremblay JÉ,Lovejoy C

    更新日期:2014-04-11 00:00:00

  • Magnesium prevents vascular calcification in vitro by inhibition of hydroxyapatite crystal formation.

    abstract::Magnesium has been shown to effectively prevent vascular calcification associated with chronic kidney disease. Magnesium has been hypothesized to prevent the upregulation of osteoblastic genes that potentially drives calcification. However, extracellular effects of magnesium on hydroxyapatite formation are largely neg...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-20241-3

    authors: Ter Braake AD,Tinnemans PT,Shanahan CM,Hoenderop JGJ,de Baaij JHF

    更新日期:2018-02-01 00:00:00

  • Otopathogenic Staphylococcus aureus Invades Human Middle Ear Epithelial Cells Primarily through Cholesterol Dependent Pathway.

    abstract::Chronic suppurative otitis media (CSOM) is one of the most common infectious diseases of the middle ear especially affecting children, leading to delay in language development and communication. Although Staphylococcus aureus is the most common pathogen associated with CSOM, its interaction with middle ear epithelial ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-47079-7

    authors: Mittal R,Debs LH,Patel AP,Nguyen D,Blackwelder P,Yan D,Weckwerth PH,Liu XZ

    更新日期:2019-07-25 00:00:00

  • Multiple and diverse structural changes affect the breakpoint regions of polymorphic inversions across the Drosophila genus.

    abstract::Chromosomal polymorphism is widespread in the Drosophila genus, with extensive evidence supporting its adaptive character in diverse species. Moreover, inversions are the major contributors to the genus chromosomal evolution. The molecular characterization of a reduced number of polymorphic inversion breakpoints in Dr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep36248

    authors: Puerma E,Orengo DJ,Aguadé M

    更新日期:2016-10-26 00:00:00

  • Downregulation of the small GTPase SAR1A: a key event underlying alcohol-induced Golgi fragmentation in hepatocytes.

    abstract::The hepatic asialoglycoprotein receptor (ASGP-R) is posttranslationally modified in the Golgi en route to the plasma membrane, where it mediates clearance of desialylated serum glycoproteins. It is known that content of plasma membrane-associated ASGP-R is decreased after ethanol exposure, although the mechanisms rema...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep17127

    authors: Petrosyan A,Cheng PW,Clemens DL,Casey CA

    更新日期:2015-11-26 00:00:00

  • Relationship between Daily and In-laboratory Gait Speed among Healthy Community-dwelling Older Adults.

    abstract::Gait speed in laboratory settings (in-laboratory gait speed) is one of the important indicators associated with the decline in functional abilities in older adulthood. Recently, it has become possible to measure gait speed during daily living (daily gait speed) using accelerometers. However, the relationship between t...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-39695-0

    authors: Takayanagi N,Sudo M,Yamashiro Y,Lee S,Kobayashi Y,Niki Y,Shimada H

    更新日期:2019-03-05 00:00:00

  • Inductively actuated micro needles for on-demand intracellular delivery.

    abstract::Methods that provide controlled influx of molecules into cells are of critical importance for uncovering cellular mechanisms, drug development and synthetic biology. However, reliable intracellular delivery without adversely affecting the cells is a major challenge. We developed a platform for on-demand intracellular ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-28194-3

    authors: Kavaldzhiev MN,Perez JE,Sougrat R,Bergam P,Ravasi T,Kosel J

    更新日期:2018-07-02 00:00:00

  • CRISRP/Cas9-mediated knockout of Mct8 reveals a functional involvement of Mct8 in testis and sperm development in a rat.

    abstract::Thyroid hormone (TH) has long been believed to play a minor role in male reproduction. However, evidences from experimental model of thyrotoxicosis or hypothyroidism suggests its role in spermatogenesis. Cellular action of TH requires membrane transport via specific transporters such as monocarboxylate transporter 8 (...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-67594-2

    authors: Bae HS,Jin YK,Ham S,Kim HK,Shin H,Cho GB,Lee KJ,Lee H,Kim KM,Koo OJ,Jang G,Lee JM,Lee JY

    更新日期:2020-07-07 00:00:00

  • Horseradish peroxidase inactivation: heme destruction and influence of polyethylene glycol.

    abstract::Horseradish peroxidase (HRP) mediates efficient conversion of many phenolic contaminants and thus has potential applications for pollution control. Such potentially important applications suffer however from the fact that the enzyme becomes quickly inactivated during phenol oxidation and polymerization. The work here ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep03126

    authors: Mao L,Luo S,Huang Q,Lu J

    更新日期:2013-11-04 00:00:00

  • Controllable thermal rectification realized in binary phase change composites.

    abstract::Phase transition is a natural phenomenon happened around our daily life, represented by the process from ice to water. While melting and solidifying at a certain temperature, a high heat of fusion is accompanied, classified as the latent heat. Phase change material (PCM) has been widely applied to store and release la...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep08884

    authors: Chen R,Cui Y,Tian H,Yao R,Liu Z,Shu Y,Li C,Yang Y,Ren T,Zhang G,Zou R

    更新日期:2015-03-09 00:00:00

  • The PRC2-binding long non-coding RNAs in human and mouse genomes are associated with predictive sequence features.

    abstract::Recently, long non-coding RNAs (lncRNAs) have emerged as an important class of molecules involved in many cellular processes. One of their primary functions is to shape epigenetic landscape through interactions with chromatin modifying proteins. However, mechanisms contributing to the specificity of such interactions ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep41669

    authors: Tu S,Yuan GC,Shao Z

    更新日期:2017-01-31 00:00:00

  • Author Correction: Ultra high dose rate (35 Gy/sec) radiation does not spare the normal tissue in cardiac and splenic models of lymphopenia and gastrointestinal syndrome.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-020-67913-7

    authors: Venkatesulu BP,Sharma A,Pollard-Larkin JM,Sadagopan R,Symons J,Neri S,Singh PK,Tailor R,Lin SH,Krishnan S

    更新日期:2020-06-30 00:00:00

  • Electrospun Fibrous Membranes with Super-large-strain Electric Superhydrophobicity.

    abstract::Large-strain elastic superhydrophobicity is highly desirable for its enhanced use performance and functional reliability in mechanically dynamic environments, but remains challenging to develop. Here we have, for the first time, proven that an elastic fibrous membrane after surface hydrophobization can maintain superh...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep15863

    authors: Zhou H,Wang H,Niu H,Lin T

    更新日期:2015-10-29 00:00:00

  • Role of agonistic autoantibodies against type-1 angiotensin II receptor in the pathogenesis of retinopathy in preeclampsia.

    abstract::To investigate the mechanism underlying AT1-AA-induced retinopathy in severe preeclampsia by measuring the positive rate and titer of AT1-AA in plasma from women with severe preeclampsia and normal pregnant women to see whether AT1-AA titer was correlated with the grade of retinopathy. A preeclampsia rat model was als...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep29036

    authors: Liu F,Wang Y,Wang X,Zheng Y,Jin Z,Zhi J

    更新日期:2016-07-06 00:00:00

  • A ParDE-family toxin antitoxin system in major resistance plasmids of Enterobacteriaceae confers antibiotic and heat tolerance.

    abstract::Toxin-antitoxin (TA) systems were initially discovered as plasmid addiction systems on low-copy-number plasmids. Thousands of TA loci have since been identified on chromosomes, plasmids and mobile elements in bacteria and archaea with diverse roles in bacterial physiology and in maintenance of genetic elements. Here, ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-46318-1

    authors: Kamruzzaman M,Iredell J

    更新日期:2019-07-08 00:00:00

  • Associations between antioxidant vitamins and the risk of invasive cervical cancer in Chinese women: A case-control study.

    abstract::Previous studies on the associations between dietary antioxidant vitamins and the risk of cervical cancer remain inconsistent, and little evidence is available for serum antioxidant vitamins, which provide more accurate measurements of these nutrients. We conducted a case-control study of 458 incident cases with invas...

    journal_title:Scientific reports

    pub_type: 杂志文章,多中心研究

    doi:10.1038/srep13607

    authors: Guo L,Zhu H,Lin C,Che J,Tian X,Han S,Zhao H,Zhu Y,Mao D

    更新日期:2015-09-04 00:00:00

  • Dampening of Submesoscale Currents by Air-Sea Stress Coupling in the Californian Upwelling System.

    abstract::Oceanic submesoscale currents (SMCs) occur on an scale of 0.1-10 km horizontally and have a large influence on the oceanic variability and on ecosystems. At the mesoscale (10-250 km), oceanic thermal and current feedbacks are known to have a significant influence on the atmosphere and on oceanic dynamics. However, air...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-31602-3

    authors: Renault L,McWilliams JC,Gula J

    更新日期:2018-09-06 00:00:00

  • Identification of strong candidate genes for backfat and intramuscular fatty acid composition in three crosses based on the Iberian pig.

    abstract::Meat quality has an important genetic component and can be modified by the fatty acid (FA) composition and the amount of fat contained in adipose tissue and muscle. The present study aimed to find genomic regions associated with the FA composition in backfat and muscle (longissimus dorsi) in 439 pigs with three differ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-70894-2

    authors: Crespo-Piazuelo D,Criado-Mesas L,Revilla M,Castelló A,Noguera JL,Fernández AI,Ballester M,Folch JM

    更新日期:2020-08-18 00:00:00