Abstract:
:Comet assay is a widely used method, especially in the field of genotoxicity, to quantify and measure DNA damage visually at the level of individual cells with high sensitivity and efficiency. Generally, computer programs are used to analyze comet assay output images following two main steps. First, each comet region must be located and segmented, and next, it is scored using common metrics (e.g., tail length and tail moment). Currently, most studies on comet assay image analysis have adopted hand-crafted features rather than the recent and effective deep learning (DL) methods. In this paper, however, we propose a DL-based baseline method, called DeepComet, for comet segmentation. Furthermore, we created a trainable and testable comet assay image dataset that contains 1037 comet assay images with 8271 manually annotated comet objects. From the comet segmentation test results with the proposed dataset, the DeepComet achieves high average precision (AP), which is an essential metric in image segmentation and detection tasks. A comparative analysis was performed between the DeepComet and the state-of-the-arts automatic comet segmentation programs on the dataset. Besides, we found that the DeepComet records high correlations with a commercial comet analysis tool, which suggests that the DeepComet is suitable for practical application.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Hong Y,Han HJ,Lee H,Lee D,Ko J,Hong ZY,Lee JY,Seok JH,Lim HS,Son WC,Sohn Idoi
10.1038/s41598-020-75592-7subject
Has Abstractpub_date
2020-11-03 00:00:00pages
18915issue
1issn
2045-2322pii
10.1038/s41598-020-75592-7journal_volume
10pub_type
杂志文章abstract::The impact of environment on individuals is particularly critical. In evolutionary games, adopting the strategy of the neighbor who performs better is nontrivial for the survival and maintenance of cooperation, in that such an action may help the agents to obtain higher benefit and more obvious evolutionary advantages...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-34116-0
更新日期:2018-10-23 00:00:00
abstract::Endoplasmic reticulum (ER) stress preconditioning protects cells against methylmercury (MeHg) cytotoxicity by inducing integrated stress responses such as eIF2α phosphorylation, ATF4 accumulation, and nonsense-mediated mRNA decay (NMD) suppression. Here we demonstrated that ER stress preconditioning results in the upr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09435-3
更新日期:2017-09-28 00:00:00
abstract::Recreational boating is an increasing activity in coastal areas and its spatiotemporal overlap with key habitats of marine species pose a risk for negative noise impacts. Yet, recreational vessels are currently unaccounted for in vessel noise models using Automatic Identification System (AIS) data. Here we conduct a c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51222-9
更新日期:2019-10-29 00:00:00
abstract::Articular cartilage (AC) lacks the ability to self-repair and cell-based approaches, primarily based on using chondrocytes and mesenchymal stem cells (MSCs), are emerging as effective technology to restore cartilage functionality, because cells synergic functionality may support the maintenance of chondrogenic phenoty...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51070-7
更新日期:2019-10-10 00:00:00
abstract::An efficient [4 + 1] annulation between α-bromooximes and sulfur ylides via in situ generation of nitrosoalkenes under mild basic reaction conditions has been developed, providing an expeditious and scalable approach to synthesize biologically interesting isoxazoline derivatives with good to excellent yields. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-81370-w
更新日期:2021-01-22 00:00:00
abstract::Phytosterols found naturally in plants are known to reduce cholesterol absorption in the gut. The traditional southern Chinese diet typically contains many vegetables and not much meat, and there is high prevalence of lactose intolerance in Chinese; we therefore aimed to test if phytosterols-enriched milk is effective...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/srep41084
更新日期:2017-01-24 00:00:00
abstract::Drought is one of the major impediments in wheat productivity. Traditional breeding and marker assisted QTL introgression had limited success. Available wheat genomic and RNA-seq data can decipher novel drought tolerance mechanisms with putative candidate gene and marker discovery. Drought is first sensed by root tiss...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49915-2
更新日期:2019-09-26 00:00:00
abstract::In this paper, we investigate the possibility of improving efficiency in non-radiative wireless power transfer (WPT) using metasurfaces embedded between two current varying coils and present a complete theoretical analysis of this system. We use a point-dipole approximation to calculate the fields of the coils. Based ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33174-8
更新日期:2018-10-05 00:00:00
abstract::We provide a unified and exact framework for the variance-based uncertainty relations. This unified framework not only recovers some well-known previous uncertainty relations, but also fixes the deficiencies of them. Utilizing the unified framework, we can construct the new uncertainty relations in both product and su...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56803-2
更新日期:2020-01-10 00:00:00
abstract::Bisacylphosphane oxides (BAPOs) are established as photoinitiators for industrial applications. Light irradiation leads to their photolysis, producing radicals. Radical species induce oxidative stress in cells and may cause cell death. Hence, BAPOs may be suitable as photolatent cytotoxic agents, but such applications...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42026-y
更新日期:2019-04-12 00:00:00
abstract::Temperature index (TI) models are convenient for modelling glacier ablation since they require only a few input variables and rely on simple empirical relations. The approach is generally assumed to be reliable at lower elevations (below 3500 m above sea level, a.s.l) where air temperature (Ta) relates well to the ene...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41657-5
更新日期:2019-03-27 00:00:00
abstract::In this study, the term "ultrasonic ureteral crossing sign" is defined, and the diagnostic accuracy of this sign in the rapid localization of ureteral calculi is assessed. Between January 2017 and June 2018, 535 patients underwent ultrasound examination for suspected ureteral calculi. The "ultrasonic ureteral crossing...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58805-x
更新日期:2020-02-05 00:00:00
abstract::Medulloblastoma is a malignant pediatric tumor that arises from neural progenitors in the cerebellum. Despite a five-year survival rate of ~70%, nearly all patients incur adverse side effects from current treatment strategies that drastically impact quality of life. Roughly one-third of medulloblastoma are driven by a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50088-1
更新日期:2019-09-20 00:00:00
abstract::Novelty seeking (NS) is a personality trait reflecting excitement in response to novel stimuli. High NS is usually a predictor of risky behaviour such as drug abuse. However, the relationships between NS and risk-related cognitive processes, including individual risk preference and the brain activation associated with...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/srep10534
更新日期:2015-06-11 00:00:00
abstract::Image classification uses computers to simulate human understanding and cognition of images by automatically categorizing images. This study proposes a faster image classification approach that parallelizes the traditional Adaboost-Backpropagation (BP) neural network using the MapReduce parallel programming model. Fir...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38201
更新日期:2016-12-01 00:00:00
abstract::The biological function of sterol glucosides (SGs), the most abundant sterol derivatives in higher plants, remains uncertain. In an effort to improve our understanding of these membrane lipids we examined phenotypes exhibited by the roots of Arabidopsis (Arabidopsis thaliana) lines carrying insertions in the UDP-Glc:s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05925-6
更新日期:2017-07-18 00:00:00
abstract::Enamel proteins form a scaffold for growing hydroxyapatite crystals during enamel formation. They are then almost completely degraded during enamel maturation, resulting in a protein content of only 1% (w/v) in mature enamel. Nevertheless, this small amount of remaining proteins has important effects on the mechanical...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15194
更新日期:2015-10-15 00:00:00
abstract::Proteins are the primary effectors of cellular function, including cellular metabolism, structural dynamics, and information processing. However, quantitative characterization of proteins at the single-cell level is challenging due to the tiny amount of protein available. Here, we present Abseq, a method to detect and...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44447
更新日期:2017-03-14 00:00:00
abstract::As a follow-up on the data presented for seals, we herein report and discuss outcomes resulting from a retrospective evaluation of harbour porpoise stranding and necropsy data from Schleswig-Holstein, Germany (n = 4463) to enable an objective evaluation of potential ecological effects of grey seal predation on porpois...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73258-y
更新日期:2020-10-01 00:00:00
abstract::A numerical method of solving for the elastic wave eigenmodes in acoustic waveguides of arbitrary cross-section is presented. Operating under the assumptions of linear, isotropic materials, it utilizes a finite-difference method on a staggered grid to solve for the acoustic eigenmodes (field and frequency) of the vect...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17511-x
更新日期:2017-12-13 00:00:00
abstract::Induction of cathelicidin-mediated antimicrobial pathway against intracellular M. tuberculosis by 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), the active form of vitamin D, has been documented in vitro. However, in in vivo studies related to inflammatory disorders, 1,25(OH)2D3 has been demonstrated to induce an anti-inflam...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34469
更新日期:2016-10-04 00:00:00
abstract::Realization of phosphor-free white-light emitters is becoming an important milestone on the road to achieve high quality and reliability in high-power white-light-emitting diodes (LEDs). However, most of reported methods have not been applied to practical use because of their difficulties and complexity. In this study...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17372
更新日期:2015-12-02 00:00:00
abstract::The Tcf/Lef family of transcription factors mediates the Wnt/β-catenin pathway that is involved in a wide range of biological processes, including vertebrate embryogenesis and diverse pathogenesis. Post-translational modifications, including phosphorylation, sumoylation and acetylation, are known to be important for t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42590
更新日期:2017-02-15 00:00:00
abstract::The accumulation of single nucleotide polymorphisms (SNPs) in the displacement loop (D-loop) of mitochondrial DNA (mtDNA) has been described in various types of cancers, and their association with cancer risk and disease outcome has been extensively identified. In the present study, we investigated the association bet...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02408
更新日期:2013-01-01 00:00:00
abstract::A scheme is presented to realize a single-photon transistor based on cavity quantum electrodynamics (QED) with Rydberg atomic ensemble. By combining the advantages of the cavity-enhanced interaction and Rydberg blockade, we achieve a high gain single-photon transistor. The numerical calculation shows that by using one...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41185-2
更新日期:2019-03-18 00:00:00
abstract::Mobility of people and goods during the Upper Paleolithic has proven difficult to reconstruct given the relative rareness of remains. Nevertheless, archaeological contexts like the Late Pleistocene horizon of Borsuka Cave (Southern Poland) represent a unique opportunity to explore patterns of objects' transportation a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67017-2
更新日期:2020-06-25 00:00:00
abstract::The natural course of intermittent exotropia and the factors affecting its control has been unclear. We aim to report the natural course of our cohort of 117 Chinese children with intermittent exotropia and to identify baseline parameters that may have predictive value in the control deterioration of the disease. The ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27113
更新日期:2016-06-03 00:00:00
abstract::We discovered a technical solution of such outstanding importance that it can trigger new approaches in silicon wet etching processing and, in particular, photovoltaic cell manufacturing. The so called inverted pyramid arrays, outperforming conventional pyramid textures and black silicon because of their superior ligh...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep10843
更新日期:2015-06-02 00:00:00
abstract::Fishes in the mesopelagic zone (200-1000 m) have recently been highlighted for potential exploitation. Here we assess global phylogeography in Maurolicus, the Pearlsides, an ecologically important group. We obtained new sequences from mitochondrial COI and nuclear ITS-2 from multiple locations worldwide, representing ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77528-7
更新日期:2020-11-25 00:00:00
abstract::Nerve fibers are known to reside within malignant tumors and the greater the neuronal density the worse prognosis for the patient. Recent discoveries using tumor bearing animal models have eluded to the autonomic nervous system having a direct effect on tumor growth and metastasis. We report the first direct and chron...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71670-y
更新日期:2020-09-09 00:00:00