Abstract：
:Activation of the cannabinoid CB1 receptor induces different cellular signaling cascades through coupling to different effector proteins (G-proteins and β-arrestins), triggering numerous therapeutic effects. Conformational changes and rearrangements at the intracellular domain of this GPCR receptor that accompany ligand binding dictate the signaling pathways. The GPCR-binding interface for G proteins has been extensively studied, whereas β-arrestin/GPCR complexes are still poorly understood. To gain knowledge in this direction, we designed peptides that mimic the motifs involved in the putative interacting region: β-arrestin1 finger loop and the transmembrane helix 7-helix 8 (TMH7-H8) elbow located at the intracellular side of the CB1 receptor. According to circular dichroism and NMR data, these peptides form a native-like, helical conformation and interact with each other in aqueous solution, in the presence of trifluoroethanol, and using zwitterionic detergent micelles as membrane mimics. These results increase our understanding of the binding mode of β-arrestin and CB1 receptor and validate minimalist approaches to structurally comprehend complex protein systems.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Morales P,Bruix M,Jiménez MAdoi
10.3390/ijms21218111subject
Has Abstractpub_date
2020-10-30 00:00:00issue
21issn
1422-0067pii
ijms21218111journal_volume
21pub_type
杂志文章abstract：:Bakanae disease (BD), caused by the fungal pathogen Fusarium fujikuroi, has become a serious threat in rice-cultivating regions worldwide. In the present study, quantitative trait locus (QTL) mapping was performed using F2 and F3 plants derived after crossing a BD-resistant and a BD-susceptible Korean japonica rice va...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20102598
更新日期：2019-05-27 00:00:00
abstract：:Partial or complete obstruction of the urinary tract is a common and challenging urological condition caused by a variety of conditions, including ureteral calculi, ureteral pelvic junction obstruction, ureteral stricture, and malignant ureteral obstruction. The condition, which may develop in patients of any age, ind...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21155490
更新日期：2020-07-31 00:00:00
abstract：:A review of the data on the modulatory action of adenosine 5'-triphosphate (ATP), the main co-transmitter with acetylcholine, and adenosine, the final ATP metabolite in the synaptic cleft, on neuromuscular transmission is presented. The effects of these endogenous modulators on pre- and post-synaptic processes are dis...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21176423
更新日期：2020-09-03 00:00:00
abstract：:Overabundance of extracellular matrix resulting from hyperproliferation of keloid fibroblasts (KFs) and dysregulation of apoptosis represents the main pathophysiology underlying keloids. High-mobility group box 1 (HMGB1) plays important roles in the regulation of cellular death. Suppression of HMGB1 inhibits autophagy...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20174134
更新日期：2019-08-24 00:00:00
abstract：:The alarming escalation of infectious diseases resistant to conventional antibiotics requires urgent global actions, including the development of new therapeutics. Antimicrobial peptides (AMPs) represent potential alternatives in the treatment of multi-drug resistant (MDR) infections. Here, we focus on Cecropins (Cecs...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20235862
更新日期：2019-11-22 00:00:00
abstract：:The Mexican axolotl salamander (Ambystoma mexicanum) is one member of a select group of vertebrate animals that have retained the amazing ability to regenerate multiple body parts. In addition to being an important model system for regeneration, the axolotl has also contributed extensively to studies of basic developm...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160922046
更新日期：2015-09-11 00:00:00
abstract：BACKGROUND:Left atrial appendage closure (LAAC) represents the interventional alternative to oral anticoagulation for stroke prevention in atrial fibrillation (AF). The metabolism of acylcarnitines was shown to affect cardiovascular diseases. This study evaluates the influence of successful LAAC on the metabolism of ac...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19020500
更新日期：2018-02-07 00:00:00
abstract：:Regulation of melanogenesis has been the focus of treatment for hyperpigmentary skin disorders. Although hesperidin is one of the most well-known, naturally occurring flavonoids with antioxidant and anti-inflammatory effect, its anti-melanogenic effect is not known. The present study aims to determine the anti-melanog...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160818384
更新日期：2015-08-07 00:00:00
abstract：:A considerable number of drugs and/or their metabolites are excreted by the kidneys through glomerular filtration and active renal tubule secretion via transporter proteins. Uptake transporters in the proximal tubule are part of the solute carrier (SLC) superfamily, and include the organic cation transporters (OCTs). ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21186627
更新日期：2020-09-10 00:00:00
abstract：:Glyphodes pyloalis Walker (Lepidoptera: Pyralididae) is a serious pest in the sericulture industry, which has caused damage and losses in recent years. With the widespread use of insecticides, the insecticide resistance of G. pyloalis has becomes increasingly apparent. In order to find other effective methods to contr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21051904
更新日期：2020-03-10 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms140918557
更新日期：2013-09-09 00:00:00
abstract：:Magnetic resonance spectroscopy (MRS) is a non-invasive method for quantitative estimation of liver fat. Knowledge of its imprecision, which comprises biological variability and measurement error, is required to design therapeutic trials with measurement of change. The role of adipocyte lipolysis in ectopic fat accumu...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17071089
更新日期：2016-07-08 00:00:00
abstract：:Galectin-9 (Gal-9) enhances tumor immunity mediated by T cells, macrophages, and dendritic cells. Its expression level in various cancers correlates with prognosis. Furthermore, Gal-9 directly induces apoptosis in various cancers; however, its mechanism of action and bioactivity has not been clarified. We evaluated Ga...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20112634
更新日期：2019-05-29 00:00:00
abstract：:Radiation therapy is one of the most important treatment modalities for thoracic tumors. Despite significant advances in radiation techniques, radiation-induced lung injury (RILI) still occurs in up to 30% of patients undergoing thoracic radiotherapy, and therefore remains the main dose-limiting obstacle. RILI is a po...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20163876
更新日期：2019-08-08 00:00:00
abstract：:Acute lung injury is characterized by the infiltration of neutrophils into lungs and the subsequent impairment of lung function. Here we explored the role of TDAG8 in lung injury induced by lipopolysaccharide (LPS) administrated intratracheally. In this model, cytokines and chemokines released from resident macrophage...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms161226145
更新日期：2015-12-04 00:00:00
abstract：:For possible future use in Photodynamic Therapy (PDT) and/or Photothermal Therapy (PTT) of cancer and screening of cancer cells a new type of ionic liquid photosensitizer -Cholinium-Purpurin-18 (Chol-Pu-18) - was synthesized and small gold (Au) nanoparticles, stabilized by this photosensitizer were prepared without ad...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms9050864
更新日期：2008-05-01 00:00:00
abstract：:In this article, we present a method for the enhanced molecular dynamics simulation of protein and DNA systems called potential of mean force (PMF)-enriched sampling. The method uses partitions derived from the potentials of mean force, which we determined from DNA and protein structures in the Protein Data Bank (PDB)...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19113405
更新日期：2018-10-30 00:00:00
abstract：:We have recently found that diabetes-induced premature senescence of retinal endothelial cells is accompanied by NOX2-NADPH oxidase-induced increases in the ureohydrolase enzyme arginase 1 (A1). Here, we used genetic strategies to determine the specific involvement of A1 in diabetes-induced endothelial cell senescence...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19041215
更新日期：2018-04-17 00:00:00
abstract：:The phytohormone auxin regulates various developmental programs in plants, including cell growth, cell division and cell differentiation. The auxin efflux carriers are essential for the auxin transport. To show an involvement of auxin transporters in the coordination of fruit development in bitter gourd, a juicy fruit...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18112343
更新日期：2017-11-06 00:00:00
abstract：:In 1993, the Ambros lab reported the cloning and developmental function of lin-4, the first microRNA [...]. ...
journal_title：International journal of molecular sciences
pub_type： 社论
doi：10.3390/ijms20081987
更新日期：2019-04-23 00:00:00
abstract：:Insulin-resistance is one of the main factors responsible for the onset and progression of Metabolic Syndrome (MetS). Among all polyphenols, the effects of flavonoids and their main food sources on insulin sensitivity have been widely evaluated in molecular and clinical studies. The aim of this review is to analyse th...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20092061
更新日期：2019-04-26 00:00:00
abstract：:Uterine fibroids (UFs) are benign tumors of the female genital tract made of the smooth muscle of the uterus. UF growth depends mostly on the influence of the steroid hormones and selected growth factors. Transforming growth factor β (TGF-βs) is a polypeptide that consists of three isoforms: TGF-β1, TGF-β2, and TGF-β3...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18112435
更新日期：2017-11-17 00:00:00
abstract：:The SGNH family esterases are highly effective biocatalysts due to their strong catalytic efficiencies, great stabilities, relatively small sizes, and ease of immobilization. Here, a novel SGNH family esterase (LaSGNH1) from Lactobacillus acidophilus NCFM, which has homologues in many Lactobacillus species, was identi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21010091
更新日期：2019-12-21 00:00:00
abstract：:The ovarian cancer biomarker CA125 has been extensively investigated over the last 30 years. The knowledge about the exact molecular nature of this protein, however, remains fragmented. This review provides an overview of the structural research regarding CA125, and presents an orthogonal verification method to confir...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms130810568
更新日期：2012-01-01 00:00:00
abstract：:Glu-1Ay, one of six genes encoding a high molecular weight glutenin subunit (HMW-GS), is frequently silenced in hexaploid common wheat. Here, an active allele of Glu-1Ay was integrated from wild emmer wheat (Triticum turgidum ssp. dicoccoides) accession D97 into the common wheat (Triticum aestivum) cultivar Chuannong ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19040923
更新日期：2018-03-21 00:00:00
abstract：:The natural rubber biosynthetic pathway is well described in Hevea, although the final stages of rubber elongation are still poorly understood. Small Rubber Particle Proteins and Rubber Elongation Factors (SRPPs and REFs) are proteins with major function in rubber particle formation and stabilization. Their correspond...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21124220
更新日期：2020-06-13 00:00:00
abstract：:This work describes the chemical synthesis, combinatorial selection, and enzymatic evaluation of peptidomimetic fluorescent substrates specific for the trypsin-like (β2) subunit of the 20S human proteasome. After deconvolution of a library comprising nearly 6000 compounds composed of peg substituted diaminopropionic a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21072396
更新日期：2020-03-31 00:00:00
abstract：:Paired box gene 3 (Pax3) and cAMP responsive element-binding protein (CREB) directly interact with the cis-acting elements on the promoter of microphthalmia-associated transcription factor isoform M (MITF-M) for transcriptional activation in the melanogenic process. Tyrosinase (Tyro) is a target gene of MITF-M, and fu...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21249631
更新日期：2020-12-17 00:00:00
abstract：:Balanced osteoclast and osteoblast activity is necessary for skeletal health, whereas unbalanced osteoclast activity causes bone loss in many skeletal conditions. A better understanding of pathways that regulate osteoclast differentiation and activity is necessary for the development of new therapies to better manage ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21031056
更新日期：2020-02-05 00:00:00
abstract：:Adiponectin is the adipokine associated with insulin sensitization, reducing liver gluconeogenesis, and increasing fatty acid oxidation and glucose uptake. Adiponectin is present in the kidneys, mainly in the arterial endothelium and smooth muscle cells, as well as in the capillary endothelium, and might be considered...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21249375
更新日期：2020-12-09 00:00:00
