Abstract：
:Inhibition of programmed death 1 (PD-1), expressed on activated T cells, can break through immune resistance and elicit durable responses in human melanoma as well as other types of cancers. Canine oral malignant melanoma is one of the most aggressive tumors bearing poor prognosis due to its high metastatic potency. However, there are few effective treatments for the advanced stages of melanoma in veterinary medicine. Only one previous study indicated the potential of the immune checkpoint inhibitor, anti-canine PD-L1 therapeutic antibody in dogs, and no anti-canine PD-1 therapeutic antibodies are currently available. Here, we developed two therapeutic antibodies, rat-dog chimeric and caninized anti-canine PD-1 monoclonal antibodies and evaluated in vitro functionality for these antibodies. Moreover, we conducted a pilot study to determine their safety profiles and clinical efficacy in spontaneously occurring canine cancers. In conclusion, the anti-canine PD-1 monoclonal antibody was relatively safe and effective in dogs with advanced oral malignant melanoma and other cancers. Thus, our study suggests that PD-1 blockade may be an attractive treatment option in canine cancers.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Igase M,Nemoto Y,Itamoto K,Tani K,Nakaichi M,Sakurai M,Sakai Y,Noguchi S,Kato M,Tsukui T,Mizuno Tdoi
10.1038/s41598-020-75533-4subject
Has Abstractpub_date
2020-10-27 00:00:00pages
18311issue
1issn
2045-2322pii
10.1038/s41598-020-75533-4journal_volume
10pub_type
杂志文章abstract：:The successful determination of reliable protein interaction networks (PINs) in several species in the post-genomic era has hitherto facilitated the quest to understanding systems and structural properties of such networks. It is envisaged that a clearer understanding of their intrinsic topological properties would el...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07628
更新日期：2015-02-27 00:00:00
abstract：:Taste receptors on enteroendocrine cells sense nutrients and transmit signals that control gut hormone release. This study aimed to investigate the amino acid (AA) sensing mechanisms of the ghrelin cell in a gastric ghrelinoma cell line, tissue segments and mice. Peptone and specific classes of amino acids stimulate g...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15725
更新日期：2015-10-29 00:00:00
abstract：:Free-living animals must make dietary choices in terms of chemical and physical properties, depending on their digestive physiology and availability of food resources. Here we comprehensively evaluated the dietary choices of proboscis monkeys (Nasalis larvatus) consuming young leaves. We analysed the data for leaf tou...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42774
更新日期：2017-02-17 00:00:00
abstract：:Freshwater reservoirs, in particular tropical ones, are an important source of methane (CH4) to the atmosphere, but current estimates are uncertain. The CH4 emitted from reservoirs is microbially produced in their sediments, but at present, the rate of CH4 formation in reservoir sediments cannot be predicted from sedi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47346-7
更新日期：2019-07-29 00:00:00
abstract：:Several proteins and peptides in saliva were shown to stimulate gingival wound repair, but the role of salivary metabolites in this process remains unexplored. In vitro gingival re-epithelialization kinetics were determined using unstimulated saliva samples from healthy individuals collected during an experimental gin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59988-z
更新日期：2020-02-20 00:00:00
abstract：:Ziziphus is a genus of spiny shrubs and small trees in the Rhamnaceae family. This group has a controversial taxonomy, with more than 200 species described, including Chinese jujube (Ziziphus jujuba Mill. var. jujuba) and Indian jujube (Z. mauritiana), as well as several other important cultivated fruit crops. Using 2...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31503
更新日期：2016-08-17 00:00:00
abstract：:PAP80-2a, purified from Pholiota adiposa mycelia, is a polysaccharide exhibiting prominent antitumor effects. However, the yield of PAP80-2a was low and its structure has not been characterized, impeding the exploration of its structure-function relationship, thus influencing the development of oral drugs for antitumo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-38251-6
更新日期：2019-02-11 00:00:00
abstract：:Given the rapidly expanding library of disease biomarkers and targeting agents, the number of unique targeted nanoparticles is growing exponentially. The high variability and expense of animal testing often makes it unfeasible to examine this large number of nanoparticles in vivo. This often leads to the investigation...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05840
更新日期：2014-07-28 00:00:00
abstract：:Manganese has long been employed as a T1-shortening agent in magnetic resonance imaging (MRI) applications, but these techniques are limited by the biotoxicity of bulk-manganese. Positron emission tomography (PET) offers superior contrast sensitivity compared with MRI, and recent preclinical PET studies employing 52gM...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03202-0
更新日期：2017-06-08 00:00:00
abstract：:Sleep, in addition to its brain restorative processes, plays an important role in memory transfer from its temporary store in the hippocampus to the more permanent storage in the neocortex. Alzheimer's disease (AD) affects memory and sleep. The aim of this study was to explore disturbances in global and local synchron...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41851-5
更新日期：2019-03-29 00:00:00
abstract：:We investigated risk factors for postoperative serious adverse events (SAEs) in elderly patients with preoperative chronic hypoxaemia undergone total hip arthroplasty (THA) or hemiarthroplasty and performed an implementation to modify and improve clinical outcome. A retrospective medical record review was performed to...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-55607-8
更新日期：2019-12-16 00:00:00
abstract：:Studies suggest that semen quality is declining globally, however, the debate remains open due to the possible effects of ethnic and geographical differences. This study aimed to explore whether semen quality of sperm donor candidates has changed in Henan Province, China from 2009 to 2019. In this retrospective study,...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67707-x
更新日期：2020-07-01 00:00:00
abstract：:In order to screen out an effective bone loss protectant from natural plant polyphenol and to elucidate the mechanism of the plant polyphenols that alleviate bone loss under simulated microgravity, the proliferation activities of 9 total polyphenol extracts from natural product (TPENP) on osteoblasts were measured. Po...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30992-8
更新日期：2018-08-24 00:00:00
abstract：:Melimine and Mel4 are chimeric cationic peptides with broad-spectrum antimicrobial activity. They have been shown to be highly biocompatible in animal models and human clinical trials. The current study examined the mechanism of action of these two antimicrobial peptides against P. aeruginosa. The effect of the peptid...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42440-2
更新日期：2019-05-08 00:00:00
abstract：:Osthole, an active coumarin isolated from Cnidium monnieri (L.) Cusson, has long been used in China as an antipruritic herbal medicine; however, the antipruitic mechanism of osthole is unknown. We studied the molecular mechanism of osthole in histamine-dependent itch by behavioral test, Ca(2+) imaging, and electrophys...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25657
更新日期：2016-05-10 00:00:00
abstract：:Multi-atlas-based segmentation (MAS) methods have demonstrated superior performance in the field of automatic image segmentation, and label fusion is an important part of MAS methods. In this paper, we propose a label fusion method that incorporates pixel greyscale probability information. The proposed method combines...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54527-x
更新日期：2019-11-29 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-019-55968-0
更新日期：2019-12-27 00:00:00
abstract：:The fungal microbiota is an important component of the human gut microbiome and may be linked to gastrointestinal disease. In this study, the fungal microbiota of biopsy samples from adenomas and adjacent tissues was characterized by deep sequencing. Ascomycota, Glomeromycota and Basidiomycota were identified as the d...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/srep07980
更新日期：2015-01-23 00:00:00
abstract：:We show that H-bonded host-guest systems associate in ionic liquids (ILs), pure salts with melting point below room temperature, in which dipole-dipole electrostatic interactions should be negligible in comparison with dipole-charge interactions. Binding constants (Ka) obtained from titrations of four H-bonded host-gu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71803-3
更新日期：2020-09-22 00:00:00
abstract：:Genetically engineered mouse cancer models allow tumors to be imaged in vivo via co-expression of a reporter gene with a tumor-initiating gene. However, differential transcriptional and translational regulation between the tumor-initiating gene and the reporter gene can result in inconsistency between the actual tumor...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08053
更新日期：2015-01-27 00:00:00
abstract：:Fishes in the mesopelagic zone (200-1000 m) have recently been highlighted for potential exploitation. Here we assess global phylogeography in Maurolicus, the Pearlsides, an ecologically important group. We obtained new sequences from mitochondrial COI and nuclear ITS-2 from multiple locations worldwide, representing ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77528-7
更新日期：2020-11-25 00:00:00
abstract：:Sense of body ownership and body representation are fundamental parts of human consciousness, but the contribution of the visual modality to their development remains unclear. We tested congenitally and late blind adults on a somatosensory version of the rubber hand illusion, and on the Aristotle illusion, in which si...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06622
更新日期：2014-10-23 00:00:00
abstract：:We propose a new express method of the correlation characterization of the particles suspended in the volume of optically transparent medium. It utilizes inline digital holography technique for obtaining two images of the adjacent layers from the investigated volume with subsequent matching of the cross-correlation fu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43840
更新日期：2017-03-02 00:00:00
abstract：:Behavioral alterations emerging after central or peripheral vision loss suggest that cerebral reorganization occurs for both the afferented and deafferented early visual cortex (EVC). We explored the functional reorganization of the central and peripheral EVC following visual field defects specifically affecting centr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43223
更新日期：2017-02-24 00:00:00
abstract：:Bioluminescence resonance energy transfer (BRET) has been a vital tool for understanding G protein-coupled receptor (GPCR) function. It has been used to investigate GPCR-protein and/or -ligand interactions as well as GPCR oligomerisation. However the utility of BRET is limited by the requirement that the fusion protei...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03486-2
更新日期：2017-06-09 00:00:00
abstract：:Antibody based bio-molecular drugs are an exciting, new avenue of drug development as an alternative to the more traditional small chemical compounds. However, the binding mechanism and the effect on the conformational ensembles of a therapeutic antibody to its peptide or protein antigen have not yet been well studied...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-58320-z
更新日期：2020-01-29 00:00:00
abstract：:The discovery of novel ligand chemotypes allows to explore uncharted regions in chemical space, thereby potentially improving synthetic accessibility, potency, and the drug-likeness of molecules. Here, we demonstrate the scaffold-hopping ability of the new Weighted Holistic Atom Localization and Entity Shape (WHALES) ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34677-0
更新日期：2018-11-07 00:00:00
abstract：:Memory performance results from plasticity, the ability to change with experience. We show that benefit from practice over a few trials, learning slope, is predictive of long-term recall and hippocampal volume across a broad age range and a long period of time, relates to memory training benefit, and is heritable. Fir...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78225-1
更新日期：2020-12-03 00:00:00
abstract：:Verteporfin (VP), a light-activated drug used in photodynamic therapy for the treatment of choroidal neovascular membranes, has also been shown to be an effective inhibitor of malignant cells. Recently, studies have demonstrated that, even without photo-activation, VP may still inhibit certain tumor cell lines, includ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07632-8
更新日期：2017-08-08 00:00:00
abstract：:Natural biochemical systems are ubiquitously organized both in space and time. Engineering the spatial organization of biochemistry has emerged as a key theme of synthetic biology, with numerous technologies promising improved biosynthetic pathway performance. One strategy, however, may produce disparate results for d...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26399-0
更新日期：2018-05-29 00:00:00
