Abstract:
:The human sweet taste receptor (T1R2) monomer-a member of the G-protein coupled receptor family that detects a wide variety of chemically and structurally diverse sweet tasting molecules, is known to pose a significant threat to human health. Protein that lack crystal structure is a challenge in structure-based protein design. This study focused on the interaction of the T1R2 monomer with rebaudioside A (Reb-A), a steviol glycoside with potential use as a natural sweetener using in-silico and biosensing methods. Herein, homology modelling, docking studies, and molecular dynamics simulations were applied to elucidate the interaction between Reb-A and the T1R2 monomer. In addition, the electrochemical sensing of the immobilised T1R2-Reb-A complex with zinc oxide nanoparticles (ZnONPs) and graphene oxide (GO) were assessed by testing the performance of multiwalled carbon nanotube (MWCNT) as an adsorbent experimentally. Results indicate a strong interaction between Reb-A and the T1R2 receptor, revealing the stabilizing interaction of the amino acids with the Reb-A by hydrogen bonds with the hydroxyl groups of the glucose moieties, along with a significant amount of hydrophobic interactions. Moreover, the presence of the MWCNT as an anchor confirms the adsorption strength of the T1R2-Reb-A complex onto the GO nanocomposite and supported with electrochemical measurements. Overall, this study could serve as a cornerstone in the development of electrochemical immunosensor for the detection of Reb-A, with applications in the food industry.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Arodola OA,Kanchi S,Hloma P,Bisetty K,Asiri AM,Inamuddindoi
10.1038/s41598-020-75123-4subject
Has Abstractpub_date
2020-10-27 00:00:00pages
18391issue
1issn
2045-2322pii
10.1038/s41598-020-75123-4journal_volume
10pub_type
杂志文章abstract::Caloric restriction (CR) is known to promote health and longevity, likely via modification of the gut microbiota (GM). However, functional and metabolic changes induced in the GM during CR are still unidentified. Here, we investigated the short- and long-term effects of CR on the rat GM using a metaproteogenomic appro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33100-y
更新日期:2018-10-03 00:00:00
abstract::Flavivirus-mediated inflammation causes neuronal death, but whether the infected neurons can evoke an innate immune response to elicit their own protection, is unknown. In an earlier study we have shown that neuronal RIG-I, play a significant role in inducing production and release of molecules that are related to inf...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00347
更新日期:2012-01-01 00:00:00
abstract::Individuals differ in their level of general anxiety as well as in their level of anxiety towards specific activities, such as mathematics and spatial tasks. Both specific anxieties correlate moderately with general anxiety, but the aetiology of their association remains unexplored. Moreover, the factor structure of s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42218
更新日期:2017-02-21 00:00:00
abstract::The predictions for a warmer and drier climate and for increased likelihood of climate extremes raise high concerns about the possible collapse of dryland ecosystems, and about the formation of new drylands where native species are less tolerant to water stress. Using a dryland-vegetation model for plant species that ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56080-z
更新日期:2019-12-20 00:00:00
abstract::For one-dimensional (1d) nonlinear atomic lattices, the models with on-site nonlinearities such as the Frenkel-Kontorova (FK) and ϕ4 lattices have normal energy transport while the models with inter-site nonlinearities such as the Fermi-Pasta-Ulam-β (FPU-β) lattice exhibit anomalous energy transport. The 1d Discrete N...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23719-2
更新日期:2018-03-28 00:00:00
abstract::Besides lifetime risks, we estimated life expectancy (LE), expected years of life lost (EYLL), lifetime costs, and cost-per-LY (life-year) stratified by different stages of esophageal cancer (EC). From the Taiwan Cancer Registry, we collected 14,420 EC to estimate the incidence rates during 2008-2014. They were follow...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60701-3
更新日期:2020-02-28 00:00:00
abstract::Functional reactivation of p53 pathway, although arduous, can potentially provide a broad-based strategy for cancer therapy owing to frequent p53 inactivation in human cancer. Using a phosphoprotein-screening array, we found that Benzyl Isothiocynate, (BITC) increases p53 phosphorylation in breast cancer cells and rev...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40070
更新日期:2017-01-10 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-66186-4
更新日期:2020-06-16 00:00:00
abstract::Adenocarcinoma, large cell carcinoma and squamous cell carcinoma are the most commonly diagnosed subtypes of non-small cell lung cancers (NSCLC). Numerous lung cancer cell types have exhibited electrotaxis under direct current electric fields (dcEF). Physiological electric fields (EF) play key roles in cancer cell mig...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-44505-8
更新日期:2019-05-30 00:00:00
abstract::Obesity is a progressive, chronic disease, which can be caused by long-term miscommunication between organs. It remains challenging to understand how chronic dysfunction in a particular tissue remotely impairs other organs to eventually imbalance organismal energy homeostasis. Here we introduce RNAi Pulse Induction (R...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43327-y
更新日期:2019-05-06 00:00:00
abstract::Metallic glass (MG) generally fails in a brittle manner under uniaxial tension loading at room temperature. The lack of plastic strain of MG is due to the severe plastic instability via the easily formed one dominate shear band. There have been several approaches to improve the ductility in MG, but achieving uniform t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03319
更新日期:2013-11-25 00:00:00
abstract::There is increasing interest in applications which use the 30 to 90 GHz frequency range, including automotive radar, 5 G cellular networks and wireless local area links. This study investigated pulsed 30-90 GHz radiation penetration into the human ear canal and tympanic membrane using computational phantoms. Modelling...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58091-7
更新日期:2020-01-30 00:00:00
abstract::The synthesis and subsequent genomic integration of DNA that is complementary to the genomes of non-retroviral RNA viruses are rarely observed. However, upon infection of various human cell lines and primary fibroblasts with the vesicular stomatitis virus (VSV), we detected DNA complementary to the VSV RNA. The VSV DN...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05074
更新日期:2014-05-30 00:00:00
abstract::Gradient Mn-La-Pt catalysts with three-layered structure of manganese dioxide (MnO2), lanthanum oxide (La2O3), and Platinum (Pt) for Li-O2 battery are prepared in this study. The mass ratio of the catalysts is respectively 5:2:3, 4:2:4, and 3:2:5 (MnO2: La2O3: Pt) which is start from the side of the electrolyte. The r...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34950
更新日期:2016-10-12 00:00:00
abstract::Chemotherapy (CTx)-induced premature ovarian failure (POF) in woman remains clinically irreversible. Amniotic fluid stem cells (AFSCs) have shown the potential to treat CTx-induced POF; however, the underlying mechanism is unclear. Here we demonstrate that AFSC-derived exosomes recapitulate the anti-apoptotic effect o...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23120
更新日期:2016-03-16 00:00:00
abstract::The purpose of this work is to make a case for epidemiological models with fractional exponent in the contribution of sub-populations to the incidence rate. More specifically, we question the standard assumption in the literature on epidemiological models, where the incidence rate dictating propagation of infections i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77849-7
更新日期:2020-11-30 00:00:00
abstract::This corrects the article DOI: 10.1038/srep40037. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/srep46991
更新日期:2018-05-22 00:00:00
abstract::The cytoskeletal filament vimentin is inherent to the endothelial phenotype and is critical for the proper function of endothelial cells in adult mice. It is unclear, however, if the presence of vimentin is necessary during differentiation to the endothelial phenotype. Here we evaluated gene and protein expression of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30814
更新日期:2016-08-02 00:00:00
abstract::It is highly important to understand the heterogeneous wettability properties of porous media for enhanced oil recovery (EOR). However, wettability measurements are still challenging in directly investigating the wettability of porous media. In this paper, we propose a multidimensional nuclear magnetic resonance (NMR)...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31803-w
更新日期:2018-09-07 00:00:00
abstract::Phaseonium is a three-level Λ quantum system, in which a coherent microwave and an optical control (pump) beams can be used to actively modulate the dielectric response. Here we propose a new metamaterial structure comprising of a periodic array of triangular phaseonium metamolecules arranged as a trefoil. We present ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21083
更新日期:2016-02-16 00:00:00
abstract::Filamentous fungi grow exclusively at their tips, where many growth-related fungal processes, such as enzyme secretion and invasion into host cells, take place. Hyphal tips are also a site of active metabolism. Understanding metabolic dynamics within the tip region is therefore important for biotechnology and medicine...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80270-9
更新日期:2021-01-14 00:00:00
abstract::Pot experiments were carried out to study the effects of rice straw (RS) and rice straw ash (RSA) on the growth of early rice and α-diversity of bacterial community in soils around rare earth mining areas of Xunwu and Xinfeng counties in South Jiangxi of China. The results showed that the exploitation of rare earth re...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67160-w
更新日期:2020-06-25 00:00:00
abstract::Soil zinc (Zn) plays a crucial role in plant growth, but excessive accumulation in the environment may lead to air, water and soil pollution. It is affected by various chemical, environmental and spatial factors. Therefore, it is important to identify the factors influencing Zn content in the landscape. The main motiv...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33745-9
更新日期:2018-10-22 00:00:00
abstract::The effects of antithrombotic drugs on random and free flap survival have been investigated in the past, but the experimental and clinical results are not in agreement. A perforator-based critical ischaemia model was used to evaluate the effects of different perioperatively administered pharmaceutical agents on tissue...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35833
更新日期:2016-10-21 00:00:00
abstract::Infectious bronchitis virus (IBV), a member of the Coronaviridae family, causes serious losses to the poultry industry. Intestinal microbiota play an important role in chicken health and contribute to the defence against colonization by invading pathogens. The aim of this study was to investigate the link between the ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60564-8
更新日期:2020-02-26 00:00:00
abstract::Surface soil moisture plays a crucial role on the terrestrial water, energy, and carbon cycles. Characterizing its variability in space and time is critical to increase our capability to forecast extreme weather events, manage water resources, and optimize agricultural practices. Global estimates of surface soil moist...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52650-3
更新日期:2019-11-06 00:00:00
abstract::To investigate confounding factors of real-time ultrasound elastography (RTE) and to evaluate the diagnostic performance of ultrasound (US)-guided FNA for thyroid nodules with indeterminate elastography compared with conventional US. This study included 244 nodules with indeterminate elastography caused by several con...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-18121-3
更新日期:2017-12-21 00:00:00
abstract::A Quality Improvement bundle was implemented with the goal of standardizing the multidisciplinary approach to delivery room management. We used a Pre-Post Quality Improvement initiative with the following aims: (1) Placement of a functioning pulse oximeter by two minutes after birth, (2) Delayed intubation, (3) Normot...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37397
更新日期:2016-11-21 00:00:00
abstract::Enhanced oil recovery using low-salinity solutions to sweep sandstone reservoirs is a widely-practiced strategy. The mechanisms governing this remain unresolved. Here, we elucidate the role of Ca2+ by combining chemical force microscopy (CFM) and molecular dynamics (MD) simulations. We probe the influence of electroly...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10327-9
更新日期:2017-09-08 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-33284-3
更新日期:2018-10-23 00:00:00