Dual function of perivascular fibroblasts in vascular stabilization in zebrafish.

Abstract:

:Blood vessels are vital to sustain life in all vertebrates. While it is known that mural cells (pericytes and smooth muscle cells) regulate vascular integrity, the contribution of other cell types to vascular stabilization has been largely unexplored. Using zebrafish, we identified sclerotome-derived perivascular fibroblasts as a novel population of blood vessel associated cells. In contrast to pericytes, perivascular fibroblasts emerge early during development, express the extracellular matrix (ECM) genes col1a2 and col5a1, and display distinct morphology and distribution. Time-lapse imaging reveals that perivascular fibroblasts serve as pericyte precursors. Genetic ablation of perivascular fibroblasts markedly reduces collagen deposition around endothelial cells, resulting in dysmorphic blood vessels with variable diameters. Strikingly, col5a1 mutants show spontaneous hemorrhage, and the penetrance of the phenotype is strongly enhanced by the additional loss of col1a2. Together, our work reveals dual roles of perivascular fibroblasts in vascular stabilization where they establish the ECM around nascent vessels and function as pericyte progenitors.

journal_name

PLoS Genet

journal_title

PLoS genetics

authors

Rajan AM,Ma RC,Kocha KM,Zhang DJ,Huang P

doi

10.1371/journal.pgen.1008800

subject

Has Abstract

pub_date

2020-10-26 00:00:00

pages

e1008800

issue

10

eissn

1553-7390

issn

1553-7404

pii

PGENETICS-D-20-00608

journal_volume

16

pub_type

杂志文章
  • TFAP2 paralogs regulate melanocyte differentiation in parallel with MITF.

    abstract::Mutations in the gene encoding transcription factor TFAP2A result in pigmentation anomalies in model organisms and premature hair graying in humans. However, the pleiotropic functions of TFAP2A and its redundantly-acting paralogs have made the precise contribution of TFAP2-type activity to melanocyte differentiation u...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1006636

    authors: Seberg HE,Van Otterloo E,Loftus SK,Liu H,Bonde G,Sompallae R,Gildea DE,Santana JF,Manak JR,Pavan WJ,Williams T,Cornell RA

    更新日期:2017-03-01 00:00:00

  • Examining the evolution of the regulatory circuit controlling secondary metabolism and development in the fungal genus Aspergillus.

    abstract::Filamentous fungi produce diverse secondary metabolites (SMs) essential to their ecology and adaptation. Although each SM is typically produced by only a handful of species, global SM production is governed by widely conserved transcriptional regulators in conjunction with other cellular processes, such as development...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1005096

    authors: Lind AL,Wisecaver JH,Smith TD,Feng X,Calvo AM,Rokas A

    更新日期:2015-03-18 00:00:00

  • Sustained post-mating response in Drosophila melanogaster requires multiple seminal fluid proteins.

    abstract::Successful reproduction is critical to pass genes to the next generation. Seminal proteins contribute to important reproductive processes that lead to fertilization in species ranging from insects to mammals. In Drosophila, the male's accessory gland is a source of seminal fluid proteins that affect the reproductive o...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.0030238

    authors: Ram KR,Wolfner MF

    更新日期:2007-12-01 00:00:00

  • COL4A1 mutations cause ocular dysgenesis, neuronal localization defects, and myopathy in mice and Walker-Warburg syndrome in humans.

    abstract::Muscle-eye-brain disease (MEB) and Walker Warburg Syndrome (WWS) belong to a spectrum of autosomal recessive diseases characterized by ocular dysgenesis, neuronal migration defects, and congenital muscular dystrophy. Until now, the pathophysiology of MEB/WWS has been attributed to alteration in dystroglycan post-trans...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1002062

    authors: Labelle-Dumais C,Dilworth DJ,Harrington EP,de Leau M,Lyons D,Kabaeva Z,Manzini MC,Dobyns WB,Walsh CA,Michele DE,Gould DB

    更新日期:2011-05-01 00:00:00

  • Checkpoint Activation of an Unconventional DNA Replication Program in Tetrahymena.

    abstract::The intra-S phase checkpoint kinase of metazoa and yeast, ATR/MEC1, protects chromosomes from DNA damage and replication stress by phosphorylating subunits of the replicative helicase, MCM2-7. Here we describe an unprecedented ATR-dependent pathway in Tetrahymena thermophila in which the essential pre-replicative comp...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1005405

    authors: Sandoval PY,Lee PH,Meng X,Kapler GM

    更新日期:2015-07-28 00:00:00

  • Autozygome sequencing expands the horizon of human knockout research and provides novel insights into human phenotypic variation.

    abstract::The use of autozygosity as a mapping tool in the search for autosomal recessive disease genes is well established. We hypothesized that autozygosity not only unmasks the recessiveness of disease causing variants, but can also reveal natural knockouts of genes with less obvious phenotypic consequences. To test this hyp...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1004030

    authors: Alsalem AB,Halees AS,Anazi S,Alshamekh S,Alkuraya FS

    更新日期:2013-01-01 00:00:00

  • RAN-binding protein 9 is involved in alternative splicing and is critical for male germ cell development and male fertility.

    abstract::As a member of the large Ran-binding protein family, Ran-binding protein 9 (RANBP9) has been suggested to play a critical role in diverse cellular functions in somatic cell lineages in vitro, and this is further supported by the neonatal lethality phenotype in Ranbp9 global knockout mice. However, the exact molecular ...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1004825

    authors: Bao J,Tang C,Li J,Zhang Y,Bhetwal BP,Zheng H,Yan W

    更新日期:2014-12-04 00:00:00

  • Unintentional miRNA ablation is a risk factor in gene knockout studies: a short report.

    abstract::One of the most powerful techniques for studying the function of a gene is to disrupt the expression of that gene using genetic engineering strategies such as targeted recombination or viral integration of gene trap cassettes. The tremendous utility of these tools was recognized this year with the awarding of the Nobe...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.0040034

    authors: Osokine I,Hsu R,Loeb GB,McManus MT

    更新日期:2008-02-01 00:00:00

  • AVPR1a and SLC6A4 gene polymorphisms are associated with creative dance performance.

    abstract::Dancing, which is integrally related to music, likely has its origins close to the birth of Homo sapiens, and throughout our history, dancing has been universally practiced in all societies. We hypothesized that there are differences among individuals in aptitude, propensity, and need for dancing that may partially be...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.0010042

    authors: Bachner-Melman R,Dina C,Zohar AH,Constantini N,Lerer E,Hoch S,Sella S,Nemanov L,Gritsenko I,Lichtenberg P,Granot R,Ebstein RP

    更新日期:2005-09-01 00:00:00

  • The microRNA mir-71 inhibits calcium signaling by targeting the TIR-1/Sarm1 adaptor protein to control stochastic L/R neuronal asymmetry in C. elegans.

    abstract::The Caenorhabditis elegans left and right AWC olfactory neurons communicate to establish stochastic asymmetric identities, AWC(ON) and AWC(OFF), by inhibiting a calcium-mediated signaling pathway in the future AWC(ON) cell. NSY-4/claudin-like protein and NSY-5/innexin gap junction protein are the two parallel signals ...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1002864

    authors: Hsieh YW,Chang C,Chuang CF

    更新日期:2012-01-01 00:00:00

  • Polymorphisms in the yeast galactose sensor underlie a natural continuum of nutrient-decision phenotypes.

    abstract::In nature, microbes often need to "decide" which of several available nutrients to utilize, a choice that depends on a cell's inherent preference and external nutrient levels. While natural environments can have mixtures of different nutrients, phenotypic variation in microbes' decisions of which nutrient to utilize i...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1006766

    authors: Lee KB,Wang J,Palme J,Escalante-Chong R,Hua B,Springer M

    更新日期:2017-05-24 00:00:00

  • RAD50 is required for efficient initiation of resection and recombinational repair at random, gamma-induced double-strand break ends.

    abstract::Resection of DNA double-strand break (DSB) ends is generally considered a critical determinant in pathways of DSB repair and genome stability. Unlike for enzymatically induced site-specific DSBs, little is known about processing of random "dirty-ended" DSBs created by DNA damaging agents such as ionizing radiation. He...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1000656

    authors: Westmoreland J,Ma W,Yan Y,Van Hulle K,Malkova A,Resnick MA

    更新日期:2009-09-01 00:00:00

  • Multiple mechanisms regulate H3 acetylation of enhancers in response to thyroid hormone.

    abstract::Hormone-dependent activation of enhancers includes histone hyperacetylation and mediator recruitment. Histone hyperacetylation is mostly explained by a bimodal switch model, where histone deacetylases (HDACs) disassociate from chromatin, and histone acetyl transferases (HATs) are recruited. This model builds on decade...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1008770

    authors: Præstholm SM,Siersbæk MS,Nielsen R,Zhu X,Hollenberg AN,Cheng SY,Grøntved L

    更新日期:2020-05-26 00:00:00

  • Histone methyltransferase DOT1L drives recovery of gene expression after a genotoxic attack.

    abstract::UV-induced DNA damage causes repression of RNA synthesis. Following the removal of DNA lesions, transcription recovery operates through a process that is not understood yet. Here we show that knocking-out of the histone methyltransferase DOT1L in mouse embryonic fibroblasts (MEF(DOT1L)) leads to a UV hypersensitivity ...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1003611

    authors: Oksenych V,Zhovmer A,Ziani S,Mari PO,Eberova J,Nardo T,Stefanini M,Giglia-Mari G,Egly JM,Coin F

    更新日期:2013-01-01 00:00:00

  • Identifying neural substrates of competitive interactions and sequence transitions during mechanosensory responses in Drosophila.

    abstract::Nervous systems have the ability to select appropriate actions and action sequences in response to sensory cues. The circuit mechanisms by which nervous systems achieve choice, stability and transitions between behaviors are still incompletely understood. To identify neurons and brain areas involved in controlling the...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1008589

    authors: Masson JB,Laurent F,Cardona A,Barré C,Skatchkovsky N,Zlatic M,Jovanic T

    更新日期:2020-02-14 00:00:00

  • Importance of Polη for damage-induced cohesion reveals differential regulation of cohesion establishment at the break site and genome-wide.

    abstract::Genome integrity depends on correct chromosome segregation, which in turn relies on cohesion between sister chromatids from S phase until anaphase. S phase cohesion, together with DNA double-strand break (DSB) recruitment of cohesin and formation of damage-induced (DI) cohesion, has previously been shown to be require...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1003158

    authors: Enervald E,Lindgren E,Katou Y,Shirahige K,Ström L

    更新日期:2013-01-01 00:00:00

  • Hard selective sweep and ectopic gene conversion in a gene cluster affording environmental adaptation.

    abstract::Among the rare colonizers of heavy-metal rich toxic soils, Arabidopsis halleri is a compelling model extremophile, physiologically distinct from its sister species A. lyrata, and A. thaliana. Naturally selected metal hypertolerance and extraordinarily high leaf metal accumulation in A. halleri both require Heavy Metal...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1003707

    authors: Hanikenne M,Kroymann J,Trampczynska A,Bernal M,Motte P,Clemens S,Krämer U

    更新日期:2013-01-01 00:00:00

  • Genome patterns of selection and introgression of haplotypes in natural populations of the house mouse (Mus musculus).

    abstract::General parameters of selection, such as the frequency and strength of positive selection in natural populations or the role of introgression, are still insufficiently understood. The house mouse (Mus musculus) is a particularly well-suited model system to approach such questions, since it has a defined history of spl...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1002891

    authors: Staubach F,Lorenc A,Messer PW,Tang K,Petrov DA,Tautz D

    更新日期:2012-01-01 00:00:00

  • Interplay between UNG and AID governs intratumoral heterogeneity in mature B cell lymphoma.

    abstract::Most B cell lymphomas originate from B cells that have germinal center (GC) experience and bear chromosome translocations and numerous point mutations. GC B cells remodel their immunoglobulin (Ig) genes by somatic hypermutation (SHM) and class switch recombination (CSR) in their Ig genes. Activation Induced Deaminase ...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1008960

    authors: Delgado P,Álvarez-Prado ÁF,Marina-Zárate E,Sernandez IV,Mur SM,de la Barrera J,Sanchez-Cabo F,Cañamero M,de Molina A,Belver L,de Yébenes VG,Ramiro AR

    更新日期:2020-12-23 00:00:00

  • A candidate gene approach identifies the CHRNA5-A3-B4 region as a risk factor for age-dependent nicotine addiction.

    abstract::People who begin daily smoking at an early age are at greater risk of long-term nicotine addiction. We tested the hypothesis that associations between nicotinic acetylcholine receptor (nAChR) genetic variants and nicotine dependence assessed in adulthood will be stronger among smokers who began daily nicotine exposure...

    journal_title:PLoS genetics

    pub_type: 杂志文章,随机对照试验

    doi:10.1371/journal.pgen.1000125

    authors: Weiss RB,Baker TB,Cannon DS,von Niederhausern A,Dunn DM,Matsunami N,Singh NA,Baird L,Coon H,McMahon WM,Piper ME,Fiore MC,Scholand MB,Connett JE,Kanner RE,Gahring LC,Rogers SW,Hoidal JR,Leppert MF

    更新日期:2008-07-11 00:00:00

  • Chromosomal Integrity after UV Irradiation Requires FANCD2-Mediated Repair of Double Strand Breaks.

    abstract::Fanconi Anemia (FA) is a rare autosomal recessive disorder characterized by hypersensitivity to inter-strand crosslinks (ICLs). FANCD2, a central factor of the FA pathway, is essential for the repair of double strand breaks (DSBs) generated during fork collapse at ICLs. While lesions different from ICLs can also trigg...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1005792

    authors: Federico MB,Vallerga MB,Radl A,Paviolo NS,Bocco JL,Di Giorgio M,Soria G,Gottifredi V

    更新日期:2016-01-14 00:00:00

  • DNA damage as a consequence of NLR activation.

    abstract::DNA damage observed during plant immune responses is reported to be an intrinsic component of plant immunity. However, other immune responses may suppress DNA damage to maintain host genome integrity. Here, we show that immunity-related DNA damage can be abrogated by preventing cell death triggered by Nucleotide-bindi...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1007235

    authors: Rodriguez E,Chevalier J,El Ghoul H,Voldum-Clausen K,Mundy J,Petersen M

    更新日期:2018-02-20 00:00:00

  • Threshold-dependent repression of SPL gene expression by miR156/miR157 controls vegetative phase change in Arabidopsis thaliana.

    abstract::Vegetative phase change is regulated by a decrease in the abundance of the miRNAs, miR156 and miR157, and the resulting increase in the expression of their targets, SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors. To determine how miR156/miR157 specify the quantitative and qualitative changes in lea...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1007337

    authors: He J,Xu M,Willmann MR,McCormick K,Hu T,Yang L,Starker CG,Voytas DF,Meyers BC,Poethig RS

    更新日期:2018-04-19 00:00:00

  • MicroRNA93 regulates proliferation and differentiation of normal and malignant breast stem cells.

    abstract::MicroRNAs (miRNAs) play important roles in normal cellular differentiation and oncogenesis. microRNA93 (mir-93), a member of the mir106b-25 cluster, located in intron 13 of the MCM7 gene, although frequently overexpressed in human malignancies may also function as a tumor suppressor gene. Using a series of breast canc...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1002751

    authors: Liu S,Patel SH,Ginestier C,Ibarra I,Martin-Trevino R,Bai S,McDermott SP,Shang L,Ke J,Ou SJ,Heath A,Zhang KJ,Korkaya H,Clouthier SG,Charafe-Jauffret E,Birnbaum D,Hannon GJ,Wicha MS

    更新日期:2012-01-01 00:00:00

  • E Unibus Plurum: genomic analysis of an experimentally evolved polymorphism in Escherichia coli.

    abstract::Microbial populations founded by a single clone and propagated under resource limitation can become polymorphic. We sought to elucidate genetic mechanisms whereby a polymorphism evolved in Escherichia coli under glucose limitation and persisted because of cross-feeding among multiple adaptive clones. Apart from a 29 k...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1000713

    authors: Kinnersley MA,Holben WE,Rosenzweig F

    更新日期:2009-11-01 00:00:00

  • Calcium-responsive transactivator (CREST) toxicity is rescued by loss of PBP1/ATXN2 function in a novel yeast proteinopathy model and in transgenic flies.

    abstract::Proteins associated with familial neurodegenerative disease often aggregate in patients' neurons. Several such proteins, e.g. TDP-43, aggregate and are toxic when expressed in yeast. Deletion of the ATXN2 ortholog, PBP1, reduces yeast TDP-43 toxicity, which led to identification of ATXN2 as an amyotrophic lateral scle...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1008308

    authors: Park S,Park SK,Watanabe N,Hashimoto T,Iwatsubo T,Shelkovnikova TA,Liebman SW

    更新日期:2019-08-07 00:00:00

  • Multilevel regulation of the glass locus during Drosophila eye development.

    abstract::Development of eye tissue is initiated by a conserved set of transcription factors termed retinal determination network (RDN). In the fruit fly Drosophila melanogaster, the zinc-finger transcription factor Glass acts directly downstream of the RDN to control identity of photoreceptor as well as non-photoreceptor cells...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1008269

    authors: Fritsch C,Bernardo-Garcia FJ,Humberg TH,Mishra AK,Miellet S,Almeida S,Frochaux MV,Deplancke B,Huber A,Sprecher SG

    更新日期:2019-07-12 00:00:00

  • Role of duplicate genes in robustness against deleterious human mutations.

    abstract::It is now widely recognized that robustness is an inherent property of biological systems [1],[2],[3]. The contribution of close sequence homologs to genetic robustness against null mutations has been previously demonstrated in simple organisms [4],[5]. In this paper we investigate in detail the contribution of gene d...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1000014

    authors: Hsiao TL,Vitkup D

    更新日期:2008-03-14 00:00:00

  • Loss of Trabid, a new negative regulator of the drosophila immune-deficiency pathway at the level of TAK1, reduces life span.

    abstract::A relatively unexplored nexus in Drosophila Immune deficiency (IMD) pathway is TGF-beta Activating Kinase 1 (TAK1), which triggers both immunity and apoptosis. In a cell culture screen, we identified that Lysine at position 142 was a K63-linked Ubiquitin acceptor site for TAK1, required for signalling. Moreover, Lysin...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1004117

    authors: Fernando MD,Kounatidis I,Ligoxygakis P

    更新日期:2014-02-20 00:00:00

  • Identification of 526 conserved metazoan genetic innovations exposes a new role for cofactor E-like in neuronal microtubule homeostasis.

    abstract::The evolution of metazoans from their choanoflagellate-like unicellular ancestor coincided with the acquisition of novel biological functions to support a multicellular lifestyle, and eventually, the unique cellular and physiological demands of differentiated cell types such as those forming the nervous, muscle and im...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1003804

    authors: Frédéric MY,Lundin VF,Whiteside MD,Cueva JG,Tu DK,Kang SY,Singh H,Baillie DL,Hutter H,Goodman MB,Brinkman FS,Leroux MR

    更新日期:2013-01-01 00:00:00