当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES期刊下所有文献 > Crystal Structure of Non-Structural Protein 10 from Severe Acute Respiratory Syndrome Coronavirus-2.

Crystal Structure of Non-Structural Protein 10 from Severe Acute Respiratory Syndrome Coronavirus-2.

Abstract：

:Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), causing Coronavirus Disease 19 (COVID-19), emerged at the end of 2019 and quickly spread to cause a global pandemic with severe socio-economic consequences. The early sequencing of its RNA genome revealed its high similarity to SARS, likely to have originated from bats. The SARS-CoV-2 non-structural protein 10 (nsp10) displays high sequence similarity with its SARS homologue, which binds to and stimulates the 3'-to-5' exoribonuclease and the 2'-O-methlytransferase activities of nsps 14 and 16, respectively. Here, we report the biophysical characterization and 1.6 Å resolution structure of the unbound form of nsp10 from SARS-CoV-2 and compare it to the structures of its SARS homologue and the complex-bound form with nsp16 from SARS-CoV-2. The crystal structure and solution behaviour of nsp10 will not only form the basis for understanding the role of SARS-CoV-2 nsp10 as a central player of the viral RNA capping apparatus, but will also serve as a basis for the development of inhibitors of nsp10, interfering with crucial functions of the replication-transcription complex and virus replication.

journal_name

Int J Mol Sci

journal_title

International journal of molecular sciences

authors

Rogstam A,Nyblom M,Christensen S,Sele C,Talibov VO,Lindvall T,Rasmussen AA,André I,Fisher Z,Knecht W,Kozielski F

doi

10.3390/ijms21197375

subject

Has Abstract

pub_date

2020-10-06 00:00:00

issue

19

issn

1422-0067

pii

ijms21197375

journal_volume

21

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES文献大全
  • The Implication of Substance P in the Development of Tendinopathy: A Case Control Study.

    abstract：:It was reported that substance P had beneficial effects in the healing of acute tendon injury. However, the relationship between substance P and degenerative tendinopathy development remains unclear. The purpose of this study was to determine the role of substance P in the pathogenesis of tendinopathy. Healthy and ten...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms18061241

    authors： Han SH,Choi W,Song J,Kim J,Lee S,Choi Y,Byun SE,Ahn T,Ahn H,Ding C,Baik L,Ward S,Ting K,Lee S

    更新日期：2017-06-09 00:00:00

  • Berberine Derivatives Suppress Cellular Proliferation and Tumorigenesis In Vitro in Human Non-Small-Cell Lung Cancer Cells.

    abstract：:Lung cancer is the leading cause of death in the world, and the most common type of lung cancer is non-small-cell lung cancer (NSCLC), accounting for 85% of lung cancer. Patients with NSCLC, when detected, are mostly in a metastatic stage, and over half of patients diagnosed with NSCLC die within one year after diagno...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21124218

    authors： Chang JM,Kam KH,Chao WY,Zhao PW,Chen SH,Chung HC,Li YZ,Wu JY,Lee YR

    更新日期：2020-06-13 00:00:00

  • Gonadal, Not Maternal, Acquisition of Duplicated pax6 Orthologs in Megalobrama Amblycephala.

    abstract：:: The highly conserved transcription factor Pax6 is involved in the development of the eyes, brain, and pancreas in vertebrates and invertebrates, whereas the additional expression pattern in other organs is still elusive. In this study, we cloned and characterized two pax6 homologs in blunt snout bream (Megalobrama a...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20071710

    authors： Pan Q,Xue T,Xia B,Luo J,Wang Q,Jiang Y,Yu M,Chen T

    更新日期：2019-04-05 00:00:00

  • MAPK Pathway under Chronic Copper Excess in Green Macroalgae (Chlorophyta): Involvement in the Regulation of Detoxification Mechanisms.

    abstract：:Following the physiological complementary/parallel Celis-Plá et al., by inhibiting extracellular signal regulated kinases (ERK), c-Jun N-terminal kinases (JNK), and cytokinin specific binding protein (p38), we assessed the role of the mitogen-activated protein kinases (MAPK) pathway in detoxification responses mediate...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20184546

    authors： Rodríguez-Rojas F,Celis-Plá PSM,Méndez L,Moenne F,Muñoz PT,Lobos MG,Díaz P,Sánchez-Lizaso JL,Brown MT,Moenne A,Sáez CA

    更新日期：2019-09-13 00:00:00

  • Argonaute and Argonaute-Bound Small RNAs in Stem Cells.

    abstract：:Small RNAs are essential for a variety of cellular functions. Argonaute (AGO) proteins are associated with all of the different classes of small RNAs, and are indispensable in small RNA-mediated regulatory pathways. AGO proteins have been identified in various types of stem cells in diverse species from plants and ani...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms17020208

    authors： Zhai L,Wang L,Teng F,Zhou L,Zhang W,Xiao J,Liu Y,Deng W

    更新日期：2016-02-04 00:00:00

  • Disease progression mediated by egr-1 associated signaling in response to oxidative stress.

    abstract：:When cellular reducing enzymes fail to shield the cell from increased amounts of reactive oxygen species (ROS), oxidative stress arises. The redox state is misbalanced, DNA and proteins are damaged and cellular transcription networks are activated. This condition can lead to the initiation and/or to the progression of...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms131013104

    authors： Pagel JI,Deindl E

    更新日期：2012-10-12 00:00:00

  • Injectable Gel Form of a Decellularized Bladder Induces Adipose-Derived Stem Cell Differentiation into Smooth Muscle Cells In Vitro.

    abstract：:Biologic scaffolds composed of extracellular matrix components have been proposed to repair and reconstruct a variety of tissues in clinical and pre-clinical studies. Injectable gels can fill and conform any three-dimensional shape and can be delivered to sites of interest by minimally invasive techniques. In this stu...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21228608

    authors： Moreno-Manzano V,Zaytseva-Zotova D,López-Mocholí E,Briz-Redón Á,Løkensgard Strand B,Serrano-Aroca Á

    更新日期：2020-11-15 00:00:00

  • Genetic Overlap between General Cognitive Function and Schizophrenia: A Review of Cognitive GWASs.

    abstract：:General cognitive (intelligence) function is substantially heritable, and is a major determinant of economic and health-related life outcomes. Cognitive impairments and intelligence decline are core features of schizophrenia which are evident before the onset of the illness. Genetic overlaps between cognitive impairme...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms19123822

    authors： Ohi K,Sumiyoshi C,Fujino H,Yasuda Y,Yamamori H,Fujimoto M,Shiino T,Sumiyoshi T,Hashimoto R

    更新日期：2018-11-30 00:00:00

  • Using a novel microRNA delivery system to inhibit osteoclastogenesis.

    abstract：:Previously, we developed a novel microRNA (miRNA) delivery system based on bacteriophage MS2 virus-like particles (MS2 VLPs). In this current study, we used this system to transport miR-146a into human peripheral blood mononuclear cells (PBMCs), and demonstrated the inhibition of osteoclastogenesis in precursors. Two ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms16048337

    authors： Yao Y,Jia T,Pan Y,Gou H,Li Y,Sun Y,Zhang R,Zhang K,Lin G,Xie J,Li J,Wang L

    更新日期：2015-04-14 00:00:00

  • The PERK-Dependent Molecular Mechanisms as a Novel Therapeutic Target for Neurodegenerative Diseases.

    abstract：:Higher prevalence of neurodegenerative diseases is strictly connected with progressive aging of the world population. Interestingly, a broad range of age-related, neurodegenerative diseases is characterized by a common pathological mechanism-accumulation of misfolded and unfolded proteins within the cells. Under certa...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms21062108

    authors： Rozpędek-Kamińska W,Siwecka N,Wawrzynkiewicz A,Wojtczak R,Pytel D,Diehl JA,Majsterek I

    更新日期：2020-03-19 00:00:00

  • T-Tubular Electrical Defects Contribute to Blunted β-Adrenergic Response in Heart Failure.

    abstract：:Alterations of the β-adrenergic signalling, structural remodelling, and electrical failure of T-tubules are hallmarks of heart failure (HF). Here, we assess the effect of β-adrenoceptor activation on local Ca(2+) release in electrically coupled and uncoupled T-tubules in ventricular myocytes from HF rats. We employ an...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms17091471

    authors： Crocini C,Coppini R,Ferrantini C,Yan P,Loew LM,Poggesi C,Cerbai E,Pavone FS,Sacconi L

    更新日期：2016-09-03 00:00:00

  • mTOR and Aging: An Old Fashioned Dress.

    abstract：:Aging is a physiologic/pathologic process characterized by a progressive impairment of cellular functions, supported by the alterations of several molecular pathways, leading to an increased cell susceptibility to injury. This deterioration is the primary risk factor for several major human pathologies. Numerous cellu...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms20112774

    authors： Stallone G,Infante B,Prisciandaro C,Grandaliano G

    更新日期：2019-06-06 00:00:00

  • Identification of NPB, NPW and Their Receptor in the Rat Heart.

    abstract：:Members of neuropeptide B/W signaling system have been predominantly detected and mapped within the CNS. In the rat, this system includes neuropeptide B (NPB), neuropeptide W (NPW) and their specific receptor NPBWR1. This signaling system has a wide spectrum of functions including a role in modulation of inflammatory ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21217827

    authors： Pandey S,Tuma Z,Peroni E,Monasson O,Papini AM,Chottova Dvorakova M

    更新日期：2020-10-22 00:00:00

  • Applications of Alternative Nucleases in the Age of CRISPR/Cas9.

    abstract：:Breakthroughs in the development of programmable site-specific nucleases, including zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), meganucleases (MNs), and most recently, the clustered regularly interspaced short palindromic repeats (CRISPR) associated proteins (including Cas9)...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms18122565

    authors： Guha TK,Edgell DR

    更新日期：2017-11-29 00:00:00

  • Transcriptome-Wide Identification and Expression Profiling Analysis of Chrysanthemum Trihelix Transcription Factors.

    abstract：:Trihelix transcription factors are thought to feature a typical DNA-binding trihelix (helix-loop-helix-loop-helix) domain that binds specifically to the GT motif, a light-responsive DNA element. Members of the trihelix family are known to function in a number of processes in plants. Here, we characterize 20 trihelix f...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms17020198

    authors： Song A,Wu D,Fan Q,Tian C,Chen S,Guan Z,Xin J,Zhao K,Chen F

    更新日期：2016-02-02 00:00:00

  • Application of the SwissDrugDesign Online Resources in Virtual Screening.

    abstract：:SwissDrugDesign is an important initiative led by the Molecular Modeling Group of the SIB Swiss Institute of Bioinformatics. This project provides a collection of freely available online tools for computer-aided drug design. Some of these web-based methods, i.e., SwissSimilarity and SwissTargetPrediction, were especia...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms20184612

    authors： Daina A,Zoete V

    更新日期：2019-09-18 00:00:00

  • Restraining Akt1 Phosphorylation Attenuates the Repair of Radiation-Induced DNA Double-Strand Breaks and Reduces the Survival of Irradiated Cancer Cells.

    abstract：:The survival kinase protein kinase B (Akt) participates in the regulation of essential subcellular processes, e.g., proliferation, growth, survival, and apoptosis, and has a documented role in promoting resistance against genotoxic stress including radiotherapy, presumably by influencing the DNA damage response and DN...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms19082233

    authors： Szymonowicz K,Oeck S,Krysztofiak A,van der Linden J,Iliakis G,Jendrossek V

    更新日期：2018-07-31 00:00:00

  • Common Transcriptional Program of Liver Fibrosis in Mouse Genetic Models and Humans.

    abstract：:Multifactorial metabolic diseases, such as non-alcoholic fatty liver disease, are a major burden to modern societies, and frequently present with no clearly defined molecular biomarkers. Herein we used system medicine approaches to decipher signatures of liver fibrosis in mouse models with malfunction in genes from un...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms22020832

    authors： Blagotinšek Cokan K,Urlep Ž,Moškon M,Mraz M,Kong XY,Eskild W,Rozman D,Juvan P,Režen T

    更新日期：2021-01-15 00:00:00

  • CD155: A Multi-Functional Molecule in Tumor Progression.

    abstract：:CD155 is an adhesion molecule belonging to the Nectin/Nectin-like family often overexpressed on tumor cells and involved in many different processes such as cell adhesion, migration and proliferation. In contrast to these pro-tumorigenic functions, CD155 is also a ligand for the activating receptor DNAM-1 expressed on...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms21030922

    authors： Molfetta R,Zitti B,Lecce M,Milito ND,Stabile H,Fionda C,Cippitelli M,Gismondi A,Santoni A,Paolini R

    更新日期：2020-01-30 00:00:00

  • Chronic Kidney Disease and Exposure to Nephrotoxic Metals.

    abstract：:Chronic kidney disease (CKD) is a common progressive disease that is typically characterized by the permanent loss of functional nephrons. As injured nephrons become sclerotic and die, the remaining healthy nephrons undergo numerous structural, molecular, and functional changes in an attempt to compensate for the loss...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms18051039

    authors： Orr SE,Bridges CC

    更新日期：2017-05-12 00:00:00

  • A Gene Signature of Survival Prediction for Kidney Renal Cell Carcinoma by Multi-Omic Data Analysis.

    abstract：:Kidney renal cell carcinoma (KIRC), which is the most common subtype of kidney cancer, has a poor prognosis and a high mortality rate. In this study, a multi-omics analysis is performed to build a multi-gene prognosis signature for KIRC. A combination of a DNA methylation analysis and a gene expression data analysis r...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20225720

    authors： Hu F,Zeng W,Liu X

    更新日期：2019-11-14 00:00:00

  • Integrated Proteomics and Metabolomics Analysis Provides Insights into Ganoderic Acid Biosynthesis in Response to Methyl Jasmonate in Ganoderma Lucidum.

    abstract：:Ganoderma lucidum is widely recognized as a medicinal basidiomycete. It was previously reported that the plant hormone methyl jasmonate (MeJA) could induce the biosynthesis of ganoderic acids (GAs), which are the main active ingredients of G. lucidum. However, the regulatory mechanism is still unclear. In this study, ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20246116

    authors： Jiang AL,Liu YN,Liu R,Ren A,Ma HY,Shu LB,Shi L,Zhu J,Zhao MW

    更新日期：2019-12-04 00:00:00

  • Pine bark and green tea concentrated extracts: antioxidant activity and comprehensive characterization of bioactive compounds by HPLC-ESI-QTOF-MS.

    abstract：:The consumption of polyphenols has frequently been associated with low incidence of degenerative diseases. Most of these natural antioxidants come from fruits, vegetables, spices, grains and herbs. For this reason, there has been increasing interest in identifying plant extract compounds. Polymeric tannins and monomer...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms151120382

    authors： de la Luz Cádiz-Gurrea M,Fernández-Arroyo S,Segura-Carretero A

    更新日期：2014-11-06 00:00:00

  • Molecular quantum spintronics: supramolecular spin valves based on single-molecule magnets and carbon nanotubes.

    abstract：:We built new hybrid devices consisting of chemical vapor deposition (CVD) grown carbon nanotube (CNT) transistors, decorated with TbPc(2) (Pc = phthalocyanine) rare-earth based single-molecule magnets (SMMs). The drafting was achieved by tailoring supramolecular π-π interactions between CNTs and SMMs. The magnetoresis...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms12106656

    authors： Urdampilleta M,Nguyen NV,Cleuziou JP,Klyatskaya S,Ruben M,Wernsdorfer W

    更新日期：2011-01-01 00:00:00

  • Epigenetic Suppression of the T-box Subfamily 2 (TBX2) in Human Non-Small Cell Lung Cancer.

    abstract：:(1) The TBX2 subfamily of transcription factors (TBXs 2, 3, 4 and 5) are markedly down-regulated in human non-small cell lung cancer (NSCLC) and exert tumor suppressor effects in lung malignancy. Yet, mechanisms underlying suppressed expression of the TBX2 subfamily in NSCLC are elusive. Here, we interrogated probable...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20051159

    authors： Nehme E,Rahal Z,Sinjab A,Khalil A,Chami H,Nemer G,Kadara H

    更新日期：2019-03-07 00:00:00

  • Mutagenic Study of Benzimidazole Derivatives with (+S9) and without (-S9) Metabolic Activation.

    abstract：:Benzimidazole derivatives have a diverse range of biological activities, including antiulcer, antihypertensive, antiviral, antifungal, anti-inflammatory, and anticancer. Despite these activities, previous studies have revealed that some of the derivatives can induce mutations. This study aimed to screen for potential ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20184324

    authors： Azahar NH,Ab Dullah SS,Abdullah R,Ahmat N,Md Akim A,Ab Hamid H

    更新日期：2019-09-04 00:00:00

  • Overexpression of S-Adenosyl-l-Methionine Synthetase 2 from Sugar Beet M14 Increased Arabidopsis Tolerance to Salt and Oxidative Stress.

    abstract：:The sugar beet monosomic addition line M14 is a unique germplasm that contains genetic materials from Beta vulgaris L. and Beta corolliflora Zoss, and shows tolerance to salt stress. Our study focuses on exploring the molecular mechanism of the salt tolerance of the sugar beet M14. In order to identify differentially ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms18040847

    authors： Ma C,Wang Y,Gu D,Nan J,Chen S,Li H

    更新日期：2017-04-18 00:00:00

  • Interference with the Cannabinoid Receptor CB1R Results in Miswiring of GnRH3 and AgRP1 Axons in Zebrafish Embryos.

    abstract：:The G protein-coupled cannabinoid receptors type 1 (CB1R) and type 2 (CB2R), and their endocannabinoid (eCBs) ligands, have been implicated in several aspects of brain wiring during development. Here we aim to assess whether interfering with CB1R affects development, neuritogenesis and pathfinding of GnRH and AgRP neu...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21010168

    authors： Zuccarini G,D'Atri I,Cottone E,Mackie K,Shainer I,Gothilf Y,Provero P,Bovolin P,Merlo GR

    更新日期：2019-12-25 00:00:00

  • Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination.

    abstract：:The bovine immune system is known for its unusual traits relating to immunoglobulin and antiviral responses. Peptidylarginine deiminases (PADs) are phylogenetically conserved enzymes that cause post-translational deimination, contributing to protein moonlighting in health and disease. PADs also regulate extracellular ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21082861

    authors： Criscitiello MF,Kraev I,Lange S

    更新日期：2020-04-19 00:00:00

  • Glucobrassicin Metabolites Ameliorate the Development of Portal Hypertension and Cirrhosis in Bile Duct-Ligated Rats.

    abstract：:Patients suffering from liver cirrhosis are often complicated with the formation of portosystemic collateral vessels, which is associated with the progression of a splanchnic hyperdynamic circulatory state. Alleviating pathological angiogenesis has thus been proposed to be a feasible treatment strategy. Indole-3-carbi...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20174161

    authors： Chang T,Ho HL,Hsu SJ,Chang CC,Tsai MH,Huo TI,Huang HC,Lee FY,Hou MC,Lee SD

    更新日期：2019-08-26 00:00:00