Assembly of the durian chloroplast genome using long PacBio reads.

Abstract:

:We have assembled the complete sequence of the Durio zibethinus chloroplast genome using long PacBio reads. Durian is a valuable commercial tree that produces durian fruit, which is popular in Southeast Asia. The chloroplast genome assembled into a single 143 kb cyclic contig that contained 111 genes. There were 46 short direct repeats (45 to 586 bp) and five short inverted repeats (63 to 169 bp). The long reads that were used for the assembly span the entire chloroplast with > 10 kb overlaps and multiple long reads join the start of the contig to the end of the contig. The durian chloroplast was found to lack the large inverted repeat that is common in chloroplast genomes. An additional 24 durian varieties were sequenced and compared to the assembly and found to also lack the large inverted repeat. There were nine SNPs among the varieties.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Shearman JR,Sonthirod C,Naktang C,Sangsrakru D,Yoocha T,Chatbanyong R,Vorakuldumrongchai S,Chusri O,Tangphatsornruang S,Pootakham W

doi

10.1038/s41598-020-73549-4

subject

Has Abstract

pub_date

2020-10-07 00:00:00

pages

15980

issue

1

issn

2045-2322

pii

10.1038/s41598-020-73549-4

journal_volume

10

pub_type

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