Abstract:
:Cartilage repair in osteoarthritic patients remains a challenge. Identifying resident or donor stem/progenitor cell populations is crucial for augmenting the low intrinsic repair potential of hyaline cartilage. Furthermore, mediating the interaction between these cells and the local immunogenic environment is thought to be critical for long term repair and regeneration. In this study we propose articular cartilage progenitor/stem cells (CPSC) as a valid alternative to bone marrow-derived mesenchymal stem cells (BMMSC) for cartilage repair strategies after trauma. Similar to BMMSC, CPSC isolated from osteoarthritic patients express stem cell markers and have chondrogenic, osteogenic, and adipogenic differentiation ability. In an in vitro 2D setting, CPSC show higher expression of SPP1 and LEP, markers of osteogenic and adipogenic differentiation, respectively. CPSC also display a higher commitment toward chondrogenesis as demonstrated by a higher expression of ACAN. BMMSC and CPSC were cultured in vitro using a previously established collagen-chondroitin sulfate 3D scaffold. The scaffold mimics the cartilage niche, allowing both cell populations to maintain their stem cell features and improve their immunosuppressive potential, demonstrated by the inhibition of activated PBMC proliferation in a co-culture setting. As a result, this study suggests articular cartilage derived-CPSC can be used as a novel tool for cellular and acellular regenerative medicine approaches for osteoarthritis (OA). In addition, the benefit of utilizing a biomimetic acellular scaffold as an advanced 3D culture system to more accurately mimic the physiological environment is demonstrated.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Bauza G,Pasto A,Mcculloch P,Lintner D,Brozovich A,Niclot FB,Khan I,Francis LW,Tasciotti E,Taraballi Fdoi
10.1038/s41598-020-73188-9subject
Has Abstractpub_date
2020-10-06 00:00:00pages
16610issue
1issn
2045-2322pii
10.1038/s41598-020-73188-9journal_volume
10pub_type
杂志文章abstract::Although recent studies focused on traditional Chinese medicine (TCM) for the treatment of refractory schizophrenia have reported that it may be beneficial, there is still lack of convincing evidence and critical meta-analytic work regarding its effectiveness as an adjunctive therapy. Therefore, we performed a meta-an...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析
doi:10.1038/s41598-018-24547-0
更新日期:2018-04-18 00:00:00
abstract::Unlike the constant nature of elastic coefficients of isotropic bulk materials, the Young's (E) and shear (μ) moduli of nano-structured (NS) gyroid metamaterials change with relative density (ρ), but at different rates depending on the cell size of the structure. These elastic behaviors displayed by E and μ cause cros...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10978-8
更新日期:2017-09-05 00:00:00
abstract::Root-knot nematodes (RKNs) are obligate biotrophic parasites that invade plant roots and engage in prolonged and intimate relationships with their hosts. Nematode secretions, some of which have immunosuppressing activity, play essential roles in successful parasitism; however, their mechanisms of action remain largely...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19443
更新日期:2016-01-22 00:00:00
abstract::A novel and simple method to fabricate microchannels is reported based on an inkjet printing of a volatile solid mold. A liquid ink -1,6 hexanediol- ejected from a piezoelectric nozzle is instantaneously frozen when touching a cooled substrate. The created mold is then poured with PDMS. Once the PDMS is crosslinked, t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53729-7
更新日期:2019-11-25 00:00:00
abstract::We used Mendelian randomization to estimate the causal effects of the liver enzymes, alanine aminotransferase (ALT), alkaline phosphatase (ALP) and gamma glutamyltransferase (GGT), on diabetes and cardiovascular disease, using genetic variants predicting these liver enzymes at genome wide significance applied to exten...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38813
更新日期:2016-12-20 00:00:00
abstract::Burkholderia terrae BS001 has previously been found to be able to disperse along with growing fungal hyphae in soil, with the type-3 secretion system having a supportive role in this movement. In this study, we focus on the role of two motility- and adherence-associated appendages, i.e. type-4 pili (T4P) and flagella....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02959-8
更新日期:2017-06-07 00:00:00
abstract::Hydroxyapatite (HA) is the principal inorganic component of bones and teeth and has been widely used as a bone repair material because of its good biocompatibility and bioactivity. Understanding the interactions between proteins and HA is crucial for designing biomaterials for bone regeneration. In this study, we eval...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15219-6
更新日期:2017-11-09 00:00:00
abstract::Recently, we reported that chemokine (C-X-C motif) receptor (CXCR)4 and atypical chemokine receptor 3 regulate α1-adrenergic receptors (α1-AR) through the formation of hetero-oligomeric complexes. Whether α1-ARs also regulate chemokine receptor function within such heteromeric receptor complexes is unknown. We observe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21096-4
更新日期:2018-02-09 00:00:00
abstract::Bose Einstein condensates of spin-1 atoms are known to exist in two different phases, both having spontaneously broken spin-rotation symmetry, a ferromagnetic and a polar condensate. Here we show that in two spatial dimensions it is possible to achieve a quantum phase transition from a polar condensate into a singlet ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-30876-x
更新日期:2018-08-20 00:00:00
abstract::We demonstrated an abnormal double-peak (annular shaped) energy deposition through dual-wavelength synthesis of the fundamental frequency (ω) and the second-harmonic frequency (2ω) of a femtosecond (fs) Ti:sapphire laser. The annular shaped distribution of the dual-wavelength fs laser was confirmed through real beam s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16374-6
更新日期:2017-12-11 00:00:00
abstract::This study aims to identify promising biomarkers for the early detection of lung cancer and evaluate the prognosis of lung cancer patients. Genome-wide mRNA expression data obtained from the Gene Expression Omnibus (GSE19188, GSE18842 and GSE40791), including 231 primary tumor samples and 210 normal samples, were used...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08615-5
更新日期:2017-08-14 00:00:00
abstract::Range-wide population studies of wide spread species are often associated with complex diversity patterns resulting from genetically divergent evolutionary significant units (ESUs). The compound evolutionary history creating such a pattern of diversity can be inferred through molecular analyses. Asparagus cochinchinen...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46275-9
更新日期:2019-07-05 00:00:00
abstract::The function of most long noncoding RNAs (lncRNAs) is unknown. However, recent studies reveal important roles of lncRNAs in regulating cancer-related pathways. Human antisense lncRNA-NKX2-1-AS1 partially overlaps the NKX2-1/TTF1 gene within chromosomal region 14q13.3. Amplification of this region and/or differential e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32793-5
更新日期:2018-09-26 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-70343-0
更新日期:2020-08-11 00:00:00
abstract::This study provides a pore-scale investigation of two-phase flow dynamics during primary drainage in a realistic heterogeneous rock sample. Using the lattice Boltzmann (LB) method, a series of three-dimensional (3D) immiscible displacement simulations are conducted and three typical flow patterns are identified and ma...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39741-x
更新日期:2019-03-04 00:00:00
abstract::Ring-opening graft polymerization (ROGP) of L-Lactide (L-LA) is a practical method of altering the physical and chemical properties of lignocellulose. Previous studies have mainly investigated cellulose and tin-based catalysts, particularly of tin(II) 2-ethylhexanoate (Sn(oct)2), at high temperatures and reported low ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00464-6
更新日期:2017-04-03 00:00:00
abstract::During the last decades, atmospheric nitrogen loading in mountain ranges of the Northern Hemisphere has increased substantially, resulting in high nitrate concentrations in many lakes. Yet, how increased nitrogen has affected denitrification, a key process for nitrogen removal, is poorly understood. We measured actual...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59759-w
更新日期:2020-02-20 00:00:00
abstract::The purposes of this study are to investigate the neural representation of a speech stimulus in the auditory system of individuals with normal hearing (NH) and those with hearing aids (HAs) and to explore the impact of noise reduction algorithms (NR) on auditory brainstem response to complex sounds (cABR). Twenty NH i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66970-2
更新日期:2020-07-01 00:00:00
abstract::Neuromuscular impairment requires adherence to a rehabilitation regimen for maximum recovery of motor function. Consumer-grade game controllers have emerged as a viable means to relay supervised physical therapy to patients' homes, thereby increasing their accessibility to healthcare. These controllers allow patients ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59927-y
更新日期:2020-02-28 00:00:00
abstract::Microfluidic channels have been implemented to detect cancer cells from blood using electrical measurement of each single cell from the sample. Every cell provided characteristic current profile based on its mechano-physical properties. Cancer cells not only showed higher translocation time and peak amplitude compared...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13031
更新日期:2015-09-16 00:00:00
abstract::Academic research on dinoflagellate, the primary causative agent of harmful algal blooms (HABs), is often hindered by the coexistence with bacteria in laboratory cultures. The development of axenic dinoflagellate cultures is challenging and no universally accepted method suit for different algal species. In this study...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80638-x
更新日期:2021-01-08 00:00:00
abstract::The promyelocytic leukemia (PML) protein is an essential component of PML nuclear bodies (PML NBs) frequently lost in cancer. PML NBs coordinate chromosomal regions via modification of nuclear proteins that in turn may regulate genes in the vicinity of these bodies. However, few PML NB-associated genes have been ident...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45038
更新日期:2017-03-23 00:00:00
abstract::The next-generation logic and memory devices using magnetic skyrmions as spintronic information carriers are frequently studied, thanks to their remarkable magnetic stability, extremely compact size and very-low-cost driving forces within nanotracks. In order to realize skyrmion-based spintronic devices, understanding...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40220-6
更新日期:2019-03-05 00:00:00
abstract::Epitope characterization is critical for elucidating the mechanism of action of drug candidates. However, traditional high-resolution epitope mapping techniques are not well suited for screening numerous drug candidates recognizing a similar target. Here, we use Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41179-0
更新日期:2019-03-18 00:00:00
abstract::Wildfires burn large parts of the tropics every year, shaping ecosystem structure and functioning. Yet the complex interplay between climate, vegetation and human factors that drives fire dynamics is still poorly understood. Here we show that on all continents, except Australia, tropical fire regimes change drasticall...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14654-9
更新日期:2017-10-30 00:00:00
abstract::Anxiety, depression, and insomnia are highly prevalent among migraineurs and are associated with negative health consequences. Anxiety and depression, however, unlike insomnia, are usually underdiagnosed, due to less self-reporting of these two conditions. The aim of the present study was to evaluate the risk of anxie...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55928-8
更新日期:2019-12-20 00:00:00
abstract::Bif-1 is a membrane-curvature inducing protein that is implicated in the regulation of autophagy and tumorigenesis. Here, we report that Bif-1 plays a critical role in regulating lipid catabolism to control the size of lipid droplets and prevent the development of obesity and insulin resistance upon aging or dietary c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20453
更新日期:2016-02-09 00:00:00
abstract::Tick-borne diseases are the most common vector-borne diseases in the United States, with serology being the primary method of diagnosis. We developed the first multiplex, array-based assay for serodiagnosis of tick-borne diseases called the TBD-Serochip. The TBD-Serochip was designed to discriminate antibody responses...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21349-2
更新日期:2018-02-16 00:00:00
abstract::This study sought to clarify the effects of 15-lipoxygenase/15-hydroxyeicosatetraenoic acid in angiogenesis and neurological functional recovery after cerebral ischaemic stroke in mice. In vivo, we performed behavioural tests to determine functional recovery after stroke. Double immunofluorescence staining of CD31 and...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46698
更新日期:2017-04-24 00:00:00
abstract::Channelrhodopsins are light-gated ion channels of green algae used for the precise temporal and spatial control of transmembrane ion fluxes. The channelrhodopsin Chrimson from Chlamydomonas noctigama allows unprecedented deep tissue penetration due to peak absorption at 590 nm. We demonstrate by electrophysiological r...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09600-8
更新日期:2017-08-30 00:00:00