Establishment and characterization of fantail goldfish fin (FtGF) cell line from goldfish, Carassius auratus for in vitro propagation of Cyprinid herpes virus-2 (CyHV-2).

Abstract:

Background:Herpesviral hematopoietic necrosis disease, caused by cyprinid herpesvirus-2 (CyHV-2), is responsible for massive mortalities in the aquaculture of goldfish, Carassius auratus. Permissive cell lines for the isolation and propagation of CyHV-2 have been established from various goldfish tissues by sacrificing the fish. Here, we report the development of a cell line, FtGF (Fantail Goldfish Fin), from caudal fin of goldfish using non-lethal sampling. We also describe a simple protocol for successful establishment and characterization of a permissive cell line through explant method and continuous propagation of CyHV-2 with high viral titer using this cell line. Methods:Caudal fin tissue samples were collected from goldfish without killing the fish. Cell culture of goldfish caudal fin cells was carried out using Leibovitz's L-15 (L-15) medium containing 20% FBS and 1X concentration of antibiotic antimycotic solution, incubated at 28 °C. Cells were characterized and origin of the cells was confirmed by sequencing fragments of the 16S rRNA and COI genes. CyHV-2 was grown in the FtGF cells and passaged continuously 20 times. The infectivity of the CyHV-2 isolated using FtGF cells was confirmed by experimental infection of naïve goldfish. Results:The cell line has been passaged up to 56 times in L-15 with 10% FBS. Karyotyping of FtGF cells at 30th, 40th and 56th passage indicated that modal chromosome number was 2n = 104. Species authentication of FtGF was performed by sequencing of the 16S rRNA and COI genes. The cell line was used for continuous propagation of CyHV-2 over 20 passages with high viral titer of 107.8±0.26 TCID50/mL. Following inoculation of CyHV-2 positive tissue homogenate, FtGF cells showed cytopathic effect by 2nd day post-inoculation (dpi) and complete destruction of cells was observed by the 10th dpi. An experimental infection of naïve goldfish using supernatant from infected FtGF cells caused 100% mortality and CyHV-2 infection in the challenged fish was confirmed by the amplification of DNA polymerase gene, histopathology and transmission electron microscopy. These findings provide confirmation that the FtGF cell line is highly permissive to the propagation of CyHV-2.

journal_name

PeerJ

journal_title

PeerJ

authors

Dharmaratnam A,Kumar R,Valaparambil BS,Sood N,Pradhan PK,Das S,Swaminathan TR

doi

10.7717/peerj.9373

subject

Has Abstract

pub_date

2020-07-14 00:00:00

pages

e9373

issn

2167-8359

pii

9373

journal_volume

8

pub_type

杂志文章

相关文献

PeerJ文献大全
  • Analysis of the in planta transcriptome expressed by the corn pathogen Pantoea stewartii subsp. stewartii via RNA-Seq.

    abstract::Pantoea stewartii subsp. stewartii is a bacterial phytopathogen that causes Stewart's wilt disease in corn. It uses quorum sensing to regulate expression of some genes involved in virulence in a cell density-dependent manner as the bacterial population grows from small numbers at the initial infection site in the leaf...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.3237

    authors: Packard H,Kernell Burke A,Jensen RV,Stevens AM

    更新日期:2017-04-27 00:00:00

  • A framework for smartphone-enabled, patient-generated health data analysis.

    abstract:BACKGROUND:Digital medicine and smartphone-enabled health technologies provide a novel source of human health and human biology data. However, in part due to its intricacies, few methods have been established to analyze and interpret data in this domain. We previously conducted a six-month interventional trial examinin...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2284

    authors: Gollamudi SS,Topol EJ,Wineinger NE

    更新日期:2016-08-02 00:00:00

  • Leaf arrangements are invalid in the taxonomy of orchid species.

    abstract::The selection and validation of proper distinguishing characters are of crucial importance in taxonomic revisions. The modern classifications of orchids utilize the molecular tools, but still the selection and identification of the material used in these studies is for the most part related to general species morpholo...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.3609

    authors: Jakubska-Busse A,Żołubak E,Łobas Z,Gola EM

    更新日期:2017-07-21 00:00:00

  • Biosorption potential of natural, pyrolysed and acid-assisted pyrolysed sugarcane bagasse for the removal of lead from contaminated water.

    abstract::Lead (Pb) is a ubiquitous pollutant which poses serious threats to plants, animals and humans once entered into the food chain via contaminated industrial effluents on their discharge into the surface of water bodies and/or geological materials. This study aimed to examine and compare the biosorption potential of natu...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.5672

    authors: Shah GM,Nasir M,Imran M,Bakhat HF,Rabbani F,Sajjad M,Umer Farooq AB,Ahmad S,Song L

    更新日期:2018-09-28 00:00:00

  • Curcumin plays a synergistic role in combination with HSV-TK/GCV in inhibiting growth of murine B16 melanoma cells and melanoma xenografts.

    abstract::Melanoma is a global concern and accounts for the major mortality of skin cancers. Herpes simplex virus thymidine kinase gene with ganciclovir (HSV-TK/GCV) is a promising gene therapy for melanoma. Despite its low efficiency, it is well known for its bystander effect which is mainly mediated by gap junction. In this s...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7760

    authors: Li H,Du H,Zhang G,Wu Y,Qiu P,Liu J,Guo J,Liu X,Sun L,Du B,Tan Y

    更新日期:2019-09-20 00:00:00

  • Assessing the utility of an institutional publications officer: a pilot assessment.

    abstract:BACKGROUND:The scholarly publication landscape is changing rapidly. We investigated whether the introduction of an institutional publications officer might help facilitate better knowledge of publication topics and related resources, and effectively support researchers to publish. METHODS:In September 2015, a purpose-...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.3294

    authors: Cobey KD,Galipeau J,Shamseer L,Moher D

    更新日期:2017-05-31 00:00:00

  • Modified generalized method of moments for a robust estimation of polytomous logistic model.

    abstract::The maximum likelihood estimation (MLE) method, typically used for polytomous logistic regression, is prone to bias due to both misclassification in outcome and contamination in the design matrix. Hence, robust estimators are needed. In this study, we propose such a method for nominal response data with continuous cov...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.467

    authors: Wang X

    更新日期:2014-07-01 00:00:00

  • Different environmental gradients affect different measures of snake β-diversity in the Amazon rainforests.

    abstract::Mechanisms generating and maintaining biodiversity at regional scales may be evaluated by quantifying β-diversity along environmental gradients. Differences in assemblages result in biotic complementarities and redundancies among sites, which may be quantified through multi-dimensional approaches incorporating taxonom...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.5628

    authors: de Fraga R,Ferrão M,Stow AJ,Magnusson WE,Lima AP

    更新日期:2018-09-24 00:00:00

  • Expression of leptin and leptin receptors in colorectal cancer-an immunohistochemical study.

    abstract::Obesity is demonstrated to be a risk factor in the development of cancers of various organs, such as colon, prostate, pancreas and so on. Leptine (LEP) is the most renowned of the adipokines. As a hormone, it mediates its effect through leptin receptor (LEPR), which is widely expressed in various tissues including col...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7624

    authors: Al-Shibli SM,Harun N,Ashour AE,Mohd Kasmuri MHB,Mizan S

    更新日期:2019-10-02 00:00:00

  • Onset of human preterm and term birth is related to unique inflammatory transcriptome profiles at the maternal fetal interface.

    abstract:BACKGROUND:Preterm birth is a main determinant of neonatal mortality and morbidity and a major contributor to the overall mortality and burden of disease. However, research of the preterm birth is hindered by the imprecise definition of the clinical phenotype and complexity of the molecular phenotype due to multiple pr...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.3685

    authors: Bukowski R,Sadovsky Y,Goodarzi H,Zhang H,Biggio JR,Varner M,Parry S,Xiao F,Esplin SM,Andrews W,Saade GR,Ilekis JV,Reddy UM,Baldwin DA

    更新日期:2017-09-01 00:00:00

  • Assessing distinct patterns of cognitive aging using tissue-specific brain age prediction based on diffusion tensor imaging and brain morphometry.

    abstract::Multimodal imaging enables sensitive measures of the architecture and integrity of the human brain, but the high-dimensional nature of advanced brain imaging features poses inherent challenges for the analyses and interpretations. Multivariate age prediction reduces the dimensionality to one biologically informative s...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.5908

    authors: Richard G,Kolskår K,Sanders AM,Kaufmann T,Petersen A,Doan NT,Monereo Sánchez J,Alnæs D,Ulrichsen KM,Dørum ES,Andreassen OA,Nordvik JE,Westlye LT

    更新日期:2018-11-30 00:00:00

  • MetaBAT, an efficient tool for accurately reconstructing single genomes from complex microbial communities.

    abstract::Grouping large genomic fragments assembled from shotgun metagenomic sequences to deconvolute complex microbial communities, or metagenome binning, enables the study of individual organisms and their interactions. Because of the complex nature of these communities, existing metagenome binning methods often miss a large...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.1165

    authors: Kang DD,Froula J,Egan R,Wang Z

    更新日期:2015-08-27 00:00:00

  • Cafeteria diet induce changes in blood flow that are more related with heat dissipation than energy accretion.

    abstract::Background. A "cafeteria" diet is a self-selected high-fat diet, providing an excess of energy, which can induce obesity. Excess of lipids in the diet hampers glucose utilization eliciting insulin resistance, which, further limits amino acid oxidation for energy. Methods. Male Wistar rats were exposed for a month to "...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2302

    authors: Sabater D,Agnelli S,Arriarán S,Romero Mdel M,Fernández-López JA,Alemany M,Remesar X

    更新日期:2016-08-03 00:00:00

  • Characteristics and outcomes of hemodialysis patients with COVID-19: a retrospective single center study.

    abstract:Background:The coronavirus 19 (COVID-19) pandemic has heightened the threat to the health and lives of patients with comorbid diseases. Infection by COVID-19 is especially detrimental to patients on hemodialysis. In this study, we evaluated the clinical characteristics, laboratory findings, treatments and prognoses of ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.10459

    authors: Luo Y,Li J,Liu Z,Yu H,Peng X,Cao C

    更新日期:2020-11-26 00:00:00

  • A systematic review of the direct and indirect effects of herbivory on plant reproduction mediated by pollination.

    abstract:Background:Plant reproduction is influenced by the net outcome of plant-herbivore and plant-pollinator interactions. While both herbivore impacts and pollinator impacts on plant reproduction have been widely studied, few studies examine them in concert. Methodology:Here, we review the contemporary literature that exam...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9049

    authors: Haas SM,Lortie CJ

    更新日期:2020-06-08 00:00:00

  • Relationship between vertical and horizontal force-velocity-power profiles in various sports and levels of practice.

    abstract::This study aimed (i) to explore the relationship between vertical (jumping) and horizontal (sprinting) force-velocity-power (FVP) mechanical profiles in a large range of sports and levels of practice, and (ii) to provide a large database to serve as a reference of the FVP profile for all sports and levels tested. A to...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.5937

    authors: Jiménez-Reyes P,Samozino P,García-Ramos A,Cuadrado-Peñafiel V,Brughelli M,Morin JB

    更新日期:2018-11-13 00:00:00

  • A novel GIS-based ensemble technique for flood susceptibility mapping using evidential belief function and support vector machine: Brisbane, Australia.

    abstract::In this study, we propose and test a novel ensemble method for improving the accuracy of each method in flood susceptibility mapping using evidential belief function (EBF) and support vector machine (SVM). The outcome of the proposed method was compared with the results of each method. The proposed method was implemen...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7653

    authors: Shafapour Tehrany M,Kumar L,Shabani F

    更新日期:2019-10-09 00:00:00

  • An adaptive scale Gaussian filter to explain White's illusion from the viewpoint of lightness assimilation for a large range of variation in spatial frequency of the grating and aspect ratio of the targets.

    abstract::The variation between the actual and perceived lightness of a stimulus has strong dependency on its background, a phenomena commonly known as lightness induction in the literature of visual neuroscience and psychology. For instance, a gray patch may perceptually appear to be darker in a background while it looks brigh...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.5626

    authors: Mitra S,Mazumdar D,Ghosh K,Bhaumik K

    更新日期:2018-09-25 00:00:00

  • Effectiveness of small road tunnels and fences in reducing amphibian roadkill and barrier effects at retrofitted roads in Sweden.

    abstract::Schemes to reduce road impacts on amphibians have been implemented for decades in Europe, yet, several aspects on the effectiveness of such schemes remain poorly understood. Particularly in northern Europe, including Sweden, there is a lack of available information on road mitigation for amphibians, which is hampering...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7518

    authors: Helldin JO,Petrovan SO

    更新日期:2019-08-26 00:00:00

  • The effect of pharmacological inhibition of Serine Proteases on neuronal networks in vitro.

    abstract::Neurons are embedded in an extracellular matrix (ECM), which functions both as a scaffold and as a regulator of neuronal function. The ECM is in turn dynamically altered through the action of serine proteases, which break down its constituents. This pathway has been implicated in the regulation of synaptic plasticity ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.6796

    authors: Van De Vijver S,Missault S,Van Soom J,Van Der Veken P,Augustyns K,Joossens J,Dedeurwaerdere S,Giugliano M

    更新日期:2019-04-23 00:00:00

  • Nursing knowledge of and attitude in cardiopulmonary arrest: cross-sectional survey analysis.

    abstract:Background:Nurses are often the first to activate the chain of survival when a cardiorespiratory arrest happens. That is why it is crucial that they keep their knowledge and skills up-to-date and their attitudes to resuscitation are very important. The main aim of this study was to analyse whether the level of theoreti...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.6410

    authors: Tíscar-González V,Blanco-Blanco J,Gea-Sánchez M,Rodriguez Molinuevo A,Moreno-Casbas T

    更新日期:2019-02-07 00:00:00

  • Transcriptome sequencing and comparative analysis of adult ovary and testis identify potential gonadal maintenance-related genes in Mauremys reevesii with temperature-dependent sex determination.

    abstract::Mauremys reevesii is a classical organism with temperature-dependent sex determination (TSD). Gonad development in early life has recently received considerable attention but gonadal maintenance after sex differentiation in turtles with TSD remains a mystery. In this study, we sequenced the transcriptomes for the adul...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.6557

    authors: Xiong L,Dong J,Jiang H,Zan J,Tong J,Liu J,Wang M,Nie L

    更新日期:2019-03-08 00:00:00

  • The homeodomain factor Gbx1 is required for locomotion and cell specification in the dorsal spinal cord.

    abstract::Dorsal horn neurons in the spinal cord integrate and relay sensory information to higher brain centers. These neurons are organized in specific laminae and different transcription factors are involved in their specification. The murine homeodomain Gbx1 protein is expressed in the mantle zone of the spinal cord at E12....

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.142

    authors: Meziane H,Fraulob V,Riet F,Krezel W,Selloum M,Geffarth M,Acampora D,Hérault Y,Simeone A,Brand M,Dollé P,Rhinn M

    更新日期:2013-08-29 00:00:00

  • Revalidation of morphological characteristics and multiplex PCR for the identification of three congener invasive Liriomyza species (Diptera: Agromyzidae) in China.

    abstract::Due to varietal differences, diminutive size, and similar morphological characters, it is difficult to classify and identify Liriomyza spp., a genus comprised of economically-important, highly-polyphagous insect pests. In this study, we reconfirmed the morphological characteristics of three closely-related invasive le...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.10138

    authors: Chang YW,Chen JY,Zheng SZ,Gao Y,Chen Y,Deng Y,Du YZ

    更新日期:2020-10-30 00:00:00

  • Effect of acute eye fatigue on cognition for young females: a pilot study.

    abstract::The number of people suffering from eye fatigue induced by visual display terminal (VDT) viewing is expected to increase in the modern world. Eye dysfunction is suggested to induce a decrease in cognitive function, at least in the long term. Furthermore, considering other previous findings, it may be reasonable to thi...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7978

    authors: Akagi R,Tonotsuka M,Horie R,Hirata K,Ando S

    更新日期:2019-10-29 00:00:00

  • Using 3D printed eggs to examine the egg-rejection behaviour of wild birds.

    abstract::The coevolutionary relationships between brood parasites and their hosts are often studied by examining the egg rejection behaviour of host species using artificial eggs. However, the traditional methods for producing artificial eggs out of plasticine, plastic, wood, or plaster-of-Paris are laborious, imprecise, and p...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.965

    authors: Igic B,Nunez V,Voss HU,Croston R,Aidala Z,López AV,Van Tatenhove A,Holford ME,Shawkey MD,Hauber ME

    更新日期:2015-05-26 00:00:00

  • Life history response of Echinops gmelinii Turcz. to variation in the rainfall pattern in a temperate desert.

    abstract:Background:Current and future changes in rainfall amount and frequency may particularly impact annual plants in desert ecosystems. The winter annual Echinops gmelinii Turcz. is widely distributed in the desert habitats of northern China and is a dominant pioneer annual plant following sand stabilization in the Tengger ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.8159

    authors: Wang Y,Li X,Liu L,Zhao J,Sun J

    更新日期:2019-11-29 00:00:00

  • Adult neurobehavioral outcome of hyperbilirubinemia in full term neonates-a 30 year prospective follow-up study.

    abstract::Background. Neonatal hyperbilirubinemia (HB) may cause severe neurological damage, but serious consequences are effectively controlled by phototherapy and blood exchange transfusion. HB is still a serious health problem in economically compromised parts of the world. The long term outcome has been regarded favorable b...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.294

    authors: Hokkanen L,Launes J,Michelsson K

    更新日期:2014-03-04 00:00:00

  • The first tetrapod from the mid-Miocene Clarkia lagerstätte (Idaho, USA).

    abstract::The Clarkia lagerstätte (Latah Formation) of Idaho is well known for its beautifully preserved plant fossils as well as a fauna of insects and fish. Here we present the first known tetrapod fossil from these deposits. This specimen, recovered from the lower anoxic zone of the beds, is preserved as a carbonaceous film ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.4880

    authors: Calede JJM,Orcutt JD,Kehl WA,Richards BD

    更新日期:2018-06-08 00:00:00

  • Hindering the illegal trade in dog and cat furs through a DNA-based protocol for species identification.

    abstract::In Western countries dogs and cats are the most popular pets, and people are increasingly opposed to their rearing for the fur industry. In 2007, a Regulation of the European Union (EU) banned the use and trade of dog and cat furs, but an official analytical protocol to identify them as source species was not provided...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.4902

    authors: Garofalo L,Mariacher A,Fanelli R,Fico R,Lorenzini R

    更新日期:2018-06-05 00:00:00