Vicarious reward unblocks associative learning about novel cues in male rats.

abstract：:Homologous recombination is widespread and catalyzes evolution. Nonetheless, its existence in animal mitochondrial DNA is questioned. We designed selections for recombination between co-resident mitochondrial genomes in various heteroplasmic Drosophila lines. In four experimental settings, recombinant genomes became t...

journal_title：eLife

authors： Ma H,O'Farrell PH

更新日期：2015-08-03 00:00:00

abstract：:As the equipment needed to perform state-of-the-art research in many areas of biology becomes ever more expensive, Eve Marder worries that researchers in less wealthy institutions might be left behind. ...

journal_title：eLife

authors： Marder E

更新日期：2013-11-19 00:00:00

abstract：:Microtubules are nucleated from specific locations at precise times in the cell cycle. However, the factors that constitute these microtubule nucleation pathways and their mode of action still need to be identified. Using purified Xenopus laevis proteins we biochemically reconstitute branching microtubule nucleation, ...

journal_title：eLife

authors： Alfaro-Aco R,Thawani A,Petry S

更新日期：2020-01-14 00:00:00

abstract：:Our understanding of the antigen presentation pathway has recently been enhanced with the identification that the tapasin-related protein TAPBPR is a second major histocompatibility complex (MHC) class I-specific chaperone. We sought to determine whether, like tapasin, TAPBPR can also influence MHC class I peptide sel...

journal_title：eLife

authors： Hermann C,van Hateren A,Trautwein N,Neerincx A,Duriez PJ,Stevanović S,Trowsdale J,Deane JE,Elliott T,Boyle LH

更新日期：2015-10-06 00:00:00

abstract：:Obesity produces a chronic inflammatory state involving the NFκB pathway, resulting in persistent elevation of the noncanonical IκB kinases IKKε and TBK1. In this study, we report that these kinases attenuate β-adrenergic signaling in white adipose tissue. Treatment of 3T3-L1 adipocytes with specific inhibitors of the...

journal_title：eLife

authors： Mowers J,Uhm M,Reilly SM,Simon J,Leto D,Chiang SH,Chang L,Saltiel AR

更新日期：2013-12-24 00:00:00

abstract：:Systemic oxygen restriction (SOR) is prevalent in numerous clinical conditions, including chronic obstructive pulmonary disease (COPD), and is associated with increased susceptibility to viral infections. However, the influence of SOR on T cell immunity remains uncharacterized. Here we show the detrimental effect of h...

journal_title：eLife

authors： Saragovi A,Abramovich I,Omar I,Arbib E,Toker O,Gottlieb E,Berger M

更新日期：2020-11-23 00:00:00

abstract：:Dietary restriction increases the longevity of many organisms, but the cell signaling and organellar mechanisms underlying this capability are unclear. We demonstrate that to permit long-term survival in response to sudden glucose depletion, yeast cells activate lipid-droplet (LD) consumption through micro-lipophagy (...

journal_title：eLife

authors： Seo AY,Lau PW,Feliciano D,Sengupta P,Gros MAL,Cinquin B,Larabell CA,Lippincott-Schwartz J

更新日期：2017-04-10 00:00:00

abstract：:Hsp90 is a homodimeric ATP-dependent molecular chaperone that remodels its substrate 'client' proteins, facilitating their folding and activating them for biological function. Despite decades of research, the mechanism connecting ATP hydrolysis and chaperone function remains elusive. Particularly puzzling has been the...

journal_title：eLife

authors： Elnatan D,Betegon M,Liu Y,Ramelot T,Kennedy MA,Agard DA

更新日期：2017-07-25 00:00:00

abstract：:The neural control of sugar consumption is critical for normal metabolism. In contrast to sugar-sensing taste neurons that promote consumption, we identify a taste neuron that limits sucrose consumption in Drosophila. Silencing of the neuron increases sucrose feeding; optogenetic activation decreases it. The feeding i...

journal_title：eLife

authors： Joseph RM,Sun JS,Tam E,Carlson JR

更新日期：2017-03-23 00:00:00

abstract：:Despite their importance in sexual differentiation and reproduction, Y chromosome genes are rarely described because they reside in repeat-rich regions that are difficult to study. Here, we show that Guy1, a unique Y chromosome gene of a major urban malaria mosquito Anopheles stephensi, confers 100% female lethality w...

journal_title：eLife

authors： Criscione F,Qi Y,Tu Z

更新日期：2016-09-20 00:00:00

abstract：:Cells deficient in the pro-death Bcl-2 family members Bax and Bak are known to be resistant to apoptotic cell death, and previous we have shown that these two effectors are also needed for mitochondrial-dependent cellular necrosis (Karch et al., 2013). Here we show that mouse embryonic fibroblasts deficient in Bax/Bak...

journal_title：eLife

authors： Karch J,Schips TG,Maliken BD,Brody MJ,Sargent MA,Kanisicak O,Molkentin JD

更新日期：2017-11-17 00:00:00

abstract：:Skeletal muscle degeneration is a complication arising from a variety of chronic diseases including advanced cancer. Pro-inflammatory cytokine TNF-α plays a pivotal role in mediating cancer-related skeletal muscle degeneration. Here, we show a novel function for retinoblastoma protein (Rb), where Rb causes sarcomeric ...

journal_title：eLife

authors： Araki K,Kawauchi K,Hirata H,Yamamoto M,Taya Y

更新日期：2013-12-03 00:00:00

abstract：:KdpFABC is an ATP-dependent K+ pump that ensures bacterial survival in K+-deficient environments. Whereas transcriptional activation of kdpFABC expression is well studied, a mechanism for down-regulation when K+ levels are restored has not been described. Here, we show that KdpFABC is inhibited when cells return to a ...

journal_title：eLife

authors： Sweet ME,Zhang X,Erdjument-Bromage H,Dubey V,Khandelia H,Neubert TA,Pedersen BP,Stokes DL

更新日期：2020-09-21 00:00:00

abstract：:Daily rhythms of food anticipatory activity (FAA) are regulated independently of the suprachiasmatic nucleus, which mediates entrainment of rhythms to light, but the neural circuits that establish FAA remain elusive. In this study, we show that mice lacking the dopamine D1 receptor (D1R KO mice) manifest greatly reduc...

journal_title：eLife

authors： Gallardo CM,Darvas M,Oviatt M,Chang CH,Michalik M,Huddy TF,Meyer EE,Shuster SA,Aguayo A,Hill EM,Kiani K,Ikpeazu J,Martinez JS,Purpura M,Smit AN,Patton DF,Mistlberger RE,Palmiter RD,Steele AD

更新日期：2014-09-12 00:00:00

abstract：:A polymorphism in the autophagy gene Atg16l1 is associated with susceptibility to inflammatory bowel disease (IBD); however, it remains unclear how autophagy contributes to intestinal immune homeostasis. Here, we demonstrate that autophagy is essential for maintenance of balanced CD4(+) T cell responses in the intesti...

journal_title：eLife

authors： Kabat AM,Harrison OJ,Riffelmacher T,Moghaddam AE,Pearson CF,Laing A,Abeler-Dörner L,Forman SP,Grencis RK,Sattentau Q,Simon AK,Pott J,Maloy KJ

更新日期：2016-02-24 00:00:00

abstract：:Actin has well established functions in cellular morphogenesis. However, it is not well understood how the various actin assemblies in a cell are kept in a dynamic equilibrium, in particular when cells have to respond to acute signals. Here, we characterize a rapid and transient actin reset in response to increased in...

journal_title：eLife

authors： Wales P,Schuberth CE,Aufschnaiter R,Fels J,García-Aguilar I,Janning A,Dlugos CP,Schäfer-Herte M,Klingner C,Wälte M,Kuhlmann J,Menis E,Hockaday Kang L,Maier KC,Hou W,Russo A,Higgs HN,Pavenstädt H,Vogl T,Roth J,Qual

更新日期：2016-12-06 00:00:00

abstract：:Mitochondria play important roles in cellular processes and disease, yet little is known about how the transcriptional regime of the mitochondrial genome varies across individuals and tissues. By analyzing >11,000 RNA-sequencing libraries across 36 tissue/cell types, we find considerable variation in mitochondrial-enc...

journal_title：eLife

authors： Ali AT,Boehme L,Carbajosa G,Seitan VC,Small KS,Hodgkinson A

更新日期：2019-02-18 00:00:00

abstract：:Goal-directed behavior involves distributed neuronal circuits in the mammalian brain, including diverse regions of neocortex. However, the cellular basis of long-range cortico-cortical signaling during goal-directed behavior is poorly understood. Here, we recorded membrane potential of excitatory layer 2/3 pyramidal n...

journal_title：eLife

authors： Yamashita T,Petersen CCh

更新日期：2016-06-21 00:00:00

abstract：:R-loops are features of chromatin consisting of a strand of DNA hybridized to RNA, as well as the expelled complementary DNA strand. R-loops are enriched at promoters where they have recently been shown to have important roles in modifying gene expression. However, the location of promoter-associated R-loops and the g...

journal_title：eLife

authors： Dumelie JG,Jaffrey SR

更新日期：2017-10-26 00:00:00

abstract：:Cytoplasmic dynein plays critical roles within the developing and mature nervous systems, including effecting nuclear migration, and retrograde transport of various cargos. Unsurprisingly, mutations in dynein are causative of various developmental neuropathies and motor neuron diseases. These 'dyneinopathies' define a...

journal_title：eLife

authors： Marzo MG,Griswold JM,Ruff KM,Buchmeier RE,Fees CP,Markus SM

更新日期：2019-07-31 00:00:00

abstract：:Unlike passive rupture of the human chorioamnion at birth, the insect extraembryonic (EE) tissues - the amnion and serosa - actively rupture and withdraw in late embryogenesis. Withdrawal is essential for development and has been a morphogenetic puzzle. Here, we use new fluorescent transgenic lines in the beetle Tribo...

journal_title：eLife

authors： Hilbrant M,Horn T,Koelzer S,Panfilio KA

更新日期：2016-01-29 00:00:00

abstract：:Toothed whales use sonar to detect, locate, and track prey. They adjust emitted sound intensity, auditory sensitivity and click rate to target range, and terminate prey pursuits with high-repetition-rate, low-intensity buzzes. However, their narrow acoustic field of view (FOV) is considered stable throughout target ap...

journal_title：eLife

authors： Wisniewska DM,Ratcliffe JM,Beedholm K,Christensen CB,Johnson M,Koblitz JC,Wahlberg M,Madsen PT

更新日期：2015-03-20 00:00:00

abstract：:In recent years, functional genomics approaches combining genetic information with bulk RNA-sequencing data have identified the downstream expression effects of disease-associated genetic risk factors through so-called expression quantitative trait locus (eQTL) analysis. Single-cell RNA-sequencing creates enormous opp...

journal_title：eLife

authors： van der Wijst M,de Vries DH,Groot HE,Trynka G,Hon CC,Bonder MJ,Stegle O,Nawijn MC,Idaghdour Y,van der Harst P,Ye CJ,Powell J,Theis FJ,Mahfouz A,Heinig M,Franke L

更新日期：2020-03-09 00:00:00

abstract：:Yeast cells undergoing the diauxic response show a striking upstream shift in poly(A) site utilization, with increased use of ORF-proximal poly(A) sites resulting in shorter 3' mRNA isoforms for most genes. This altered poly(A) pattern is extremely similar to that observed in cells containing Pol II derivatives with s...

journal_title：eLife

authors： Geisberg JV,Moqtaderi Z,Struhl K

更新日期：2020-08-26 00:00:00

abstract：:The integration of direct bottom-up inputs with contextual information is a core feature of neocortical circuits. In area V1, neurons may reduce their firing rates when their receptive field input can be predicted by spatial context. Gamma-synchronized (30-80 Hz) firing may provide a complementary signal to rates, ref...

journal_title：eLife

authors： Peter A,Uran C,Klon-Lipok J,Roese R,van Stijn S,Barnes W,Dowdall JR,Singer W,Fries P,Vinck M

更新日期：2019-02-04 00:00:00

abstract：:The emergence of subpopulations that perform distinct metabolic roles has been observed in populations of genetically identical bacteria. ...

journal_title：eLife

authors： Dal Co A,Brannon C,Ackermann M

更新日期：2018-06-29 00:00:00

abstract：:Different coding strategies are used to represent odor information at various stages of the mammalian olfactory system. A temporal latency code represents odor identity in olfactory bulb (OB), but this temporal information is discarded in piriform cortex (PCx) where odor identity is instead encoded through ensemble me...

journal_title：eLife

authors： Stern M,Bolding KA,Abbott LF,Franks KM

更新日期：2018-03-29 00:00:00

abstract：:Wnt plays important role during development and in various diseases. Because Wnts are lipidated and highly hydrophobic, they can only be purified in the presence of detergents, limiting their use in various in vitro and in vivo assays. We purified N-terminally tagged recombinant Wnt3a secreted from cells and accidenta...

journal_title：eLife

authors： Mihara E,Hirai H,Yamamoto H,Tamura-Kawakami K,Matano M,Kikuchi A,Sato T,Takagi J

更新日期：2016-02-23 00:00:00

abstract：:We recently reported that the C2AB portion of Synaptotagmin 1 (Syt1) could self-assemble into Ca(2+)-sensitive ring-like oligomers on membranes, which could potentially regulate neurotransmitter release. Here we report that analogous ring-like oligomers assemble from the C2AB domains of other Syt isoforms (Syt2, Syt7,...

journal_title：eLife

authors： Zanetti MN,Bello OD,Wang J,Coleman J,Cai Y,Sindelar CV,Rothman JE,Krishnakumar SS

更新日期：2016-07-19 00:00:00

abstract：:During outbreaks of high-consequence pathogens, airport screening programs have been deployed to curtail geographic spread of infection. The effectiveness of screening depends on several factors, including pathogen natural history and epidemiology, human behavior, and characteristics of the source epidemic. We develop...

journal_title：eLife

authors： Gostic KM,Kucharski AJ,Lloyd-Smith JO

更新日期：2015-02-19 00:00:00