Mesenchymal stem cell‑derived extracellular vesicles prevent neural stem cell hypoxia injury via promoting miR‑210‑3p expression.

Abstract:

:Neural stem cells (NSCs) have the potential to give rise to offspring cells and hypoxic injury can impair the function of NSCs. The present study investigated the effects of mesenchymal stem cell (MSC)‑derived extracellular vesicles (EVs) on NSC injury, as well as the underlying mechanisms. MSC‑EVs were isolated and identified via morphological and particle size analysis. Cobalt chloride was used to establish a hypoxic injury model in NSCs. Terminal deoxynucleotidyl transferase dUTP nick end labeling assay was conducted to detect apoptosis. Reverse transcription‑quantitative PCR was performed to detect the expression levels of miR‑210‑3p, and western blotting was used to detect the expression levels of apoptosis‑inducing factor (AIF) and Bcl‑2 19 kDa interacting protein (BNIP3). Compared with the control group, NSC apoptosis, and the expression of miR‑210‑3p, AIF and BNIP3 were significantly higher in the cobalt chloride‑induced hypoxia group. By contrast, treatment with MSC‑EVs further increased miR‑210‑3p expression levels, but reduced NSC apoptosis and the expression levels of AIF and BNIP3 compared with the model group (P<0.05). In addition, miR‑210‑3p inhibitor reduced miR‑210‑3p expression, but promoted hypoxia‑induced apoptosis and the expression levels of AIF and BNIP3 compared with the model group (P<0.05). Collectively, the results suggested that MSC‑EVs prevented NSC hypoxia injury by promoting miR‑210‑3p expression, which might reduce AIF and BNIP3 expression levels and NSC apoptosis.

journal_name

Mol Med Rep

authors

Li F,Zhang J,Liao R,Duan Y,Tao L,Xu Y,Chen A

doi

10.3892/mmr.2020.11454

subject

Has Abstract

pub_date

2020-11-01 00:00:00

pages

3813-3821

issue

5

eissn

1791-2997

issn

1791-3004

journal_volume

22

pub_type

杂志文章
  • mRNA expression of interleukins and Th1/Th2 imbalance in patients with pulmonary embolism.

    abstract::Few studies have investigated the changes of Th1- and Th2-type cytokines in pulmonary embolism (PE) patients. In this study, the gene expression of interleukins and the balance of Th1- and Th2-type cytokines in the peripheral blood mononuclear cells (PBMCs) of PE patients and controls were investigated. A total of 20 ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2012.1142

    authors: Duan Q,Lv W,Wang L,Gong Z,Wang Q,Song H,Wang H

    更新日期:2013-01-01 00:00:00

  • Interleukin-11, an interleukin-6-like cytokine, is a promising predictor for bladder cancer prognosis.

    abstract::Numerous studies have suggested that interleukin 11 (IL-11) has roles in human gastric, prostate and bone cancer and endometrial carcinoma. Hence, we evaluated the expression of IL-11 in bladder cancer and the correlation of IL-11 levels and clinico-pathological features. The expression of IL-11 in primary human bladd...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2012.1199

    authors: Wu D,Tao J,Ding J,Qu P,Lu Q,Zhang W

    更新日期:2013-02-01 00:00:00

  • Th17 cells are associated with the Th1/Th2‑cell balance during Echinococcus multilocularis infection.

    abstract::The present study investigated the immunopathological effect of Echinococcus multilocularis (Em) using cytokine detection. Expression of the T‑helper (Th) 17‑cytokine, interleukin 17 (IL‑17), was observed using immunohistochemical staining, and levels of cytokines, including IL‑17, transforming growth factor β1 (TGF‑β...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2170

    authors: Ma X,Wang L,Zhao H,Pang N,Zhang F,Jiang T,Liu X,Mamuti W,Wen H,Ding J

    更新日期:2014-07-01 00:00:00

  • Gene expression profile identifies potential biomarkers for human intervertebral disc degeneration.

    abstract::The present study aimed to reveal the potential genes associated with the pathogenesis of intervertebral disc degeneration (IDD) by analyzing microarray data using bioinformatics. Gene expression profiles of two regions of the intervertebral disc were compared between patients with IDD and controls. GSE70362 containin...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7741

    authors: Guo W,Zhang B,Li Y,Duan HQ,Sun C,Xu YQ,Feng SQ

    更新日期:2017-12-01 00:00:00

  • Bioinformatic analysis of the potential molecular mechanism of PAK7 expression in glioblastoma.

    abstract::The present study aimed to determine the potential molecular mechanisms underlying p21 (RAC1)‑activated kinase 7 (PAK7) expression in glioblastoma (GBM) and evaluate candidate prognosis biomarkers for GBM. Gene expression data from patients with GBM, including 144 tumor samples and 5 normal brain samples, were downloa...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11206

    authors: Wang X,Liu S,Shao Z,Zhang P

    更新日期:2020-08-01 00:00:00

  • Cortical lateralization in stroke patients measured by event‑related potentials during motor imagery.

    abstract::Stroke is a leading cause of impairment and disability worldwide, and motor imagery (MI) has been used in stroke rehabilitation. Electroencephalography (EEG) has been used to study MI. However, the characteristic features of EEG during MI in stroke patients have not been established. The purpose of this study was to i...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1705

    authors: Gong W,Zhang T,Shan L

    更新日期:2013-12-01 00:00:00

  • Enrichment and characterization of cancer stem‑like cells from a cervical cancer cell line.

    abstract::Cancer stem cells (CSCs) are proposed to be responsible for tumor recurrence, metastasis and the high mortality rate of cancer patients. Isolation and identification of CSCs is crucial for basic and preclinical studies. However, as there are currently no universal markers for the isolation and identification of CSCs i...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2063

    authors: Wang L,Guo H,Lin C,Yang L,Wang X

    更新日期:2014-06-01 00:00:00

  • Downregulation of miR‑193a‑3p via targeting cyclin D1 in thyroid cancer.

    abstract::Thyroid cancer (TC) is a frequently occurring malignant tumor with a rising steadily incidence. microRNA (miRNA/miR)‑193a‑3p is an miRNA that is associated with tumors, playing a crucial role in the genesis and progression of various cancers. However, the expression levels of miR‑193a‑3p and its molecular mechanisms i...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11310

    authors: Li XJ,Wen R,Wen DY,Lin P,Pan DH,Zhang LJ,He Y,Shi L,Qin YY,Lai YH,Lai JN,Yang JL,Lai QQ,Wang J,Ma J,Yang H,Pang YY

    更新日期:2020-09-01 00:00:00

  • Altered expression of genes involved in brain energy metabolism as adaptive responses in rats exposed to chronic variable stress; changes in cortical level of glucogenic and neuroactive amino acids.

    abstract::Brain metabolism is closely associated with neuronal activity and enables the accurate synthesis and function of neurotransmitters. Although previous studies have demonstrated that chronic stress is associated with the overproduction of reactive oxygen species (ROS), which leads to oxidative stress and the disruption ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.9865

    authors: Herbet M,Natorska-Chomicka D,Korga A,Ostrowska M,Izdebska M,Gawrońska-Grzywacz M,Piątkowska-Chmiel I,Pawłowski K,Ślaska B,Poleszak E,Dudka J

    更新日期:2019-03-01 00:00:00

  • Electroacupuncture ameliorates cognitive impairment through inhibition of NF-κB-mediated neuronal cell apoptosis in cerebral ischemia-reperfusion injured rats.

    abstract::Cognitive impairment is a serious mental deficit following stroke that severely affects the quality of life of stroke survivors. Nuclear factor‑κB (NF-κB)-mediated neuronal cell apoptosis is involved in the development of post-stroke cognitive impairment; therefore, it has become a promising target for the treatment o...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1392

    authors: Feng X,Yang S,Liu J,Huang J,Peng J,Lin J,Tao J,Chen L

    更新日期:2013-05-01 00:00:00

  • β-Elemene inhibits proliferation through crosstalk between glia maturation factor β and extracellular signal‑regulated kinase 1/2 and impairs drug resistance to temozolomide in glioblastoma cells.

    abstract::β-elemene, a plant-derived drug extracted from Curcuma wenyujin, has demonstrated marked antiproliferative effects on glioblastoma, while toxicity remains low. However, the underlying molecular mechanisms of the antitumor activity of β-elemene remain to be elucidated. Previously, it was identified that the glia matura...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2273

    authors: Zhu TZ,Li XM,Luo LH,Xu YH,Cao P,Liu Y,Liang GB

    更新日期:2014-08-01 00:00:00

  • Synergistic increase in the sensitivity of osteosarcoma cells to thermochemotherapy with combination of paclitaxel and etoposide.

    abstract::Osteosarcoma is a malignant bone tumor which is found most commonly in adolescents and young adults. Local perfusion thermochemotherapy has long been proposed as an alternative strategy for the treatment of osteosarcoma. As a standard anticancer drug, paclitaxel plays a significant ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2012.1058

    authors: Huang T,Gong WH,Li XC,Zou CP,Jiang GJ,Li XH,Feng DP

    更新日期:2012-11-01 00:00:00

  • Protective effect of 3,5‑dicaffeoyl‑epi‑quinic acid against UVB‑induced photoaging in human HaCaT keratinocytes.

    abstract::Derivatives of caffeoylquinic acid (CQA) have been studied and reported as potent bioactive molecules possessing various health benefits including antioxidant and anti‑inflammatory activities. In the present study, the protective effect of 3,5‑dicaffeoyl‑epi‑quinic acid (DCEQA) isolated from Atriplex gmelinii on UVB‑i...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10258

    authors: Oh JH,Karadeniz F,Lee JI,Seo Y,Kong CS

    更新日期:2019-07-01 00:00:00

  • The amyloid-β isoform pattern in cerebrospinal fluid in familial PSEN1 M139T- and L286P-associated Alzheimer's disease.

    abstract::There are several familial forms of Alzheimer's disease (AD) most of which are caused by mutations in the genes that encode the presenilin enzymes involved in the production of amyloid-β (Aβ) from the amyloid precursor protein (APP). In AD, Aβ forms fibrils that are deposited in the...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2012.774

    authors: Portelius E,Fortea J,Molinuevo JL,Gustavsson MK,Andreasson U,Sanchez-Valle R

    更新日期:2012-04-01 00:00:00

  • Effects of fenofibrate on Semaphorin 6B gene expression in rat skeletal muscle.

    abstract::Semaphorin 6B is a member of the semaphorin family of signaling proteins, which is implicated in a variety of biological processes, such as axon guidance and muscle regeneration. PPARα is known to be a regulator of semaphorin 6B in human cancer cell lines. In this study, we examined the effect of fenofibrate as a PPAR...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2011.463

    authors: Murad H,Kasies F,Azroony R,Alya G,Madania A

    更新日期:2011-05-01 00:00:00

  • Local intra‑articular injection of vascular endothelial growth factor accelerates articular cartilage degeneration in rat osteoarthritis model.

    abstract::In the pathophysiology of osteoarthritis (OA), articular cartilage degeneration exhibits a significant role. Vascular endothelial growth factor (VEGF) is considered to be an effective angiogenic factor and a crucial regulator of articular cartilage degeneration in the development of OA. Therefore, the present study ai...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.8652

    authors: Deng S,Zhou JL,Peng H,Fang HS,Liu F,Weng JQ

    更新日期:2018-05-01 00:00:00

  • Histone acetylation is involved in TCDD‑induced cleft palate formation in fetal mice.

    abstract::The aim of the present was to evaluate the effects of DNA methylation and histone acetylation on 2,3,7,8‑tetrachlorodibenzo‑p‑dioxin (TCDD)‑induced cleft palate in fetal mice. Pregnant mice (n=10) were randomly divided into two groups: i) TCDD group, mice were treated with 28 µg/kg TCDD on gestation day (GD) 10 by ora...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5348

    authors: Yuan X,Qiu L,Pu Y,Liu C,Zhang X,Wang C,Pu W,Fu Y

    更新日期:2016-08-01 00:00:00

  • B7-H4 expression in human infiltrating ductal carcinoma‑associated macrophages.

    abstract::B7-H4 is a co‑inhibitory molecule of the B7 family, which is expressed on antigen‑presenting cells (APCs) and is able to limit the T‑cell immune response. Macrophages act as professional APCs and are important for immunoregulation of the tumor microenvironment in breast cancer. In order to identify the association bet...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5510

    authors: Liu L,Li D,Chen S,Zhao R,Pang D,Li D,Fu Z

    更新日期:2016-09-01 00:00:00

  • AlloDerm implants for prevention of Frey syndrome after parotidectomy: a systematic review and meta-analysis.

    abstract::Although Frey syndrome is not life-threatening, it is identified as the most serious and widely recognized sequela of parotidectomy and has significant potential negative social and psychological implications. Several studies have investigated whether AlloDerm® implants prevent Frey syndrome effectively and safely, ho...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章,meta分析,评审

    doi:10.3892/mmr.2012.762

    authors: Zeng XT,Tang XJ,Wang XJ,Li MZ,Guo Y,Huang W,Niu YM,Leng WD

    更新日期:2012-04-01 00:00:00

  • Upregulation of microRNA‑16 alters the response to inhaled β‑agonists in patients with asthma though modulating expression of ADRB2.

    abstract::MicroRNAs (miRNAs) are non‑coding RNAs of ~22 nucleotides in length, which serve an important role in numerous diseases. Asthma is a chronic airway inflammatory disease, which is the most common chronic disease among children. The role of miRNA (miR)‑16 in asthma is unclear. The objective of the present study was to e...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10097

    authors: Yu B,Yao L,Liu C,Tang L,Xing T

    更新日期:2019-05-01 00:00:00

  • Cullin7 promotes epithelial‑mesenchymal transition of esophageal carcinoma via the ERK‑SNAI2 signaling pathway.

    abstract::Cullin7 (CUL7) is a member of Cullin protein family and exhibits a tumor‑promoting role in several types of tumors, including breast, liver and ovarian. However, its roles in esophageal carcinoma (EC) have not yet been reported. In the present study, CUL7 expression in EC tissue was revealed to be significantly higher...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.8503

    authors: Tian P,Liu D,Sun L,Sun H

    更新日期:2018-04-01 00:00:00

  • Combination of bortezomib and daunorubicin in the induction of apoptosis in T-cell acute lymphoblastic leukemia.

    abstract::Despite advances in the treatment of T‑cell acute lymphoblastic leukemia (T‑ALL), the outcome of T‑ALL treatment remains unsatisfactory, therefore, more effective treatment is urgently required. The present study examined the cytotoxicities of bortezomib in combination with daunorubicin against human Jurkat and Molt‑4...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6554

    authors: Du X,Tong J,Lu H,He C,Du S,Jia P,Zhao W,Xu H,Li J,Shen Z,Wu Y,Tong J,Zhou L

    更新日期:2017-07-01 00:00:00

  • Prognostic factors for renal allograft survival in patients with immunoglobulin A nephropathy: a case control study.

    abstract::The renal allograft survival rates of patients with immunoglobulin A nephropathy (IgAN), and patients with or without other glomerular diseases, have yet to be fully elucidated. In this study, the clinicopathological factors associated with long-term allograft survival for the prognosis of renal allograft recipients w...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.1954

    authors: Guo JQ,Song BL,Wu ZX,Wu WZ,Luo LT,Chen XW,He FQ,Zheng ZY,Yang SL,Tan JM

    更新日期:2014-04-01 00:00:00

  • Protective effects of ginsenoside Rg1 on aging Sca-1⁺ hematopoietic cells.

    abstract::In adults, bone hematopoietic cells are responsible for the lifelong production of all blood cells. It is affected in aging, with progressive loss of physiological integrity leading to impaired function by cellular intrinsic and extrinsic factors. However, intervention measures, which directly inhibit the aging of hem...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3884

    authors: Zhou Y,Liu J,Cai S,Liu D,Jiang R,Wang Y

    更新日期:2015-09-01 00:00:00

  • De novo transcriptome analysis and gene expression profiling of fish scales isolated from Carassius auratus during space flight: Impact of melatonin on gene expression in response to space radiation.

    abstract::Astronauts are inevitably exposed to two major risks during space flight, microgravity and radiation. Exposure to microgravity has been discovered to lead to rapid and vigorous bone loss due to elevated osteoclastic activity. In addition, long‑term exposure to low‑dose‑rate space radiation was identified to promote DN...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11363

    authors: Furusawa Y,Yamamoto T,Hattori A,Suzuki N,Hirayama J,Sekiguchi T,Tabuchi Y

    更新日期:2020-10-01 00:00:00

  • Ketamine‑induced bladder dysfunction is associated with extracellular matrix accumulation and impairment of calcium signaling in a mouse model.

    abstract::Due to the rising abuse of ketamine usage in recent years, ketamine‑induced urinary tract syndrome has received increasing attention. The present study aimed to investigate the molecular mechanism underlying ketamine‑associated cystitis in a mouse model. Female C57BL/6 mice were randomly divided into two groups: One g...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.9907

    authors: Shen CH,Wang ST,Wang SC,Lin SM,Lin LC,Dai YC,Liu YW

    更新日期:2019-04-01 00:00:00

  • Single-prolonged stress induces increased phosphorylation of extracellular signal-regulated kinase in a rat model of post-traumatic stress disorder.

    abstract::The extracellular signal-regulated kinase (ERK) signaling transduction pathway has been implicated in multiple physiological processes. It is not clear whether the ERK1/2 pathway participates in post-traumatic stress disorder (PTSD). The aim of this study was to provide novel insights into the mechanisms of how the am...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2011.459

    authors: Xiao B,Han F,Wang HT,Shi YX

    更新日期:2011-05-01 00:00:00

  • Effects of various doses of atorvastatin on vascular endothelial cell apoptosis and autophagy in vitro.

    abstract::Atorvastatin (Lipitor™) is a lipid‑lowering agent that is widely used in the treatment of cardiovascular diseases. Previous research has largely focused on its cholesterol‑lowering effects; however, a limited number of studies have investigated the actions of atorvastatin on vascular endothelial cells. In the present ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.9828

    authors: Zhao WB,Fu H,Chang F,Liu J,Wang J,Li F,Zhao J

    更新日期:2019-03-01 00:00:00

  • MicroRNA‑27a‑3p promotes epithelial‑mesenchymal transition by targeting NOVA alternative splicing regulator 1 in gastric cancer.

    abstract::NOVA alternative splicing regulator 1 (NOVA1) dysregulation has been detected in the gastric cancer microenvironment. Decreased NOVA1 expression has been linked to the progression and poor prognosis of gastric cancer; however, the role of NOVA1 in regulating epithelial‑mesenchymal transition (EMT) remains unclear in t...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.10949

    authors: Li K,Zhu X,Chen X,Wang X

    更新日期:2020-03-01 00:00:00

  • Combined kidney‑liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL‑6/Stat3 signaling.

    abstract::A limited number of studies have revealed that adding kidneys to liver perfusion may maintain an improved physiological balance; however, the underlying mechanism remains to be elucidated. The preset study confirmed the protective role of this new model and investigated the underlying mechanisms. Methods: A total of 1...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10379

    authors: Li J,Jia J,He N,Jiang L,Yu H,Li H,Peng Y,Xie H,Zhou L,Zheng S

    更新日期:2019-08-01 00:00:00