Proteomics Analysis of Plasma Membrane Fractions of the Root, Leaf, and Flower of Rice.

Abstract:

:The plasma membrane regulates biological processes such as ion transport, signal transduction, endocytosis, and cell differentiation/proliferation. To understand the functional characteristics and organ specificity of plasma membranes, plasma membrane protein fractions from rice root, etiolated leaf, green leaf, developing leaf sheath, and flower were analyzed by proteomics. Among the proteins identified, 511 were commonly accumulated in the five organs, whereas 270, 132, 359, 146, and 149 proteins were specifically accumulated in the root, etiolated leaf, green leaf, developing leaf sheath, and developing flower, respectively. The principle component analysis revealed that the functions of the plasma membrane in the root was different from those of green and etiolated leaves and that the plasma membrane protein composition of the leaf sheath was similar to that of the flower, but not that of the green leaf. Functional classification revealed that the root plasma membrane has more transport-related proteins than the leaf plasma membrane. Furthermore, the leaf sheath and flower plasma membranes were found to be richer in proteins involved in signaling and cell function than the green leaf plasma membrane. To validate the proteomics data, immunoblot analysis was carried out, focusing on four heterotrimeric G protein subunits, Gα, Gβ, Gγ1, and Gγ2. All subunits could be detected by both methods and, in particular, Gγ1 and Gγ2 required concentration by immunoprecipitation for mass spectrometry detection.

journal_name

Int J Mol Sci

authors

Iwasaki Y,Itoh T,Hagi Y,Matsuta S,Nishiyama A,Chaya G,Kobayashi Y,Miura K,Komatsu S

doi

10.3390/ijms21196988

subject

Has Abstract

pub_date

2020-09-23 00:00:00

issue

19

issn

1422-0067

pii

ijms21196988

journal_volume

21

pub_type

杂志文章
  • Biogenesis and mechanism of action of small non-coding RNAs: insights from the point of view of structural biology.

    abstract::Non-coding RNAs are dominant in the genomic output of the higher organisms being not simply occasional transcripts with idiosyncratic functions, but constituting an extensive regulatory network. Among all the species of non-coding RNAs, small non-coding RNAs (miRNAs, siRNAs and piRNAs) have been shown to be in the cor...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms130810268

    authors: Costa MC,Leitão AL,Enguita FJ

    更新日期:2012-01-01 00:00:00

  • Innovative Therapeutic Strategies for Effective Treatment of Brain Metastases.

    abstract::Brain metastases are the most prevalent of intracranial malignancies. They are associated with a very poor prognosis and near 100% mortality. This has been the case for decades, largely because we lack effective therapeutics to augment surgery and radiotherapy. Notwithstanding improvements in the precision and efficac...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20061280

    authors: Lim M,Puttick S,Houston ZH,Thurecht KJ,Kalita-de Croft P,Mahler S,Rose SE,Jeffree RL,Mazzieri R,Dolcetti R,Lakhani SR,Saunus JM

    更新日期:2019-03-14 00:00:00

  • Effect of S-Allyl -L-Cysteine on MCF-7 Cell Line 3-Mercaptopyruvate Sulfurtransferase/Sulfane Sulfur System, Viability and Apoptosis.

    abstract::The S-Allyl-L-cysteine ​​(SAC) component of aged garlic extract (AGE) is proven to have anticancer, antihepatotoxic, neuroprotective and neurotrophic properties. -Cystathionase (CTH), cystathionine β-synthase (CBS) and 3-mercaptopyruvate sulfurtransferase (MPST) are involved in H2S/sulfane sulfur endogenous formation ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21031090

    authors: Bronowicka-Adamska P,Bentke A,Lasota M,Wróbel M

    更新日期:2020-02-06 00:00:00

  • Novel strategies for drug discovery based on Intrinsically Disordered Proteins (IDPs).

    abstract::Intrinsically disordered proteins (IDPs) are proteins that usually do not adopt well-defined native structures when isolated in solution under physiological conditions. Numerous IDPs have close relationships with human diseases such as tumor, Parkinson disease, Alzheimer disease, diabetes, and so on. These disease-ass...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms12053205

    authors: Wang J,Cao Z,Zhao L,Li S

    更新日期:2011-01-01 00:00:00

  • Designer Biopolymers: Self-Assembling Proteins and Nucleic Acids.

    abstract::Nature has evolved sequence-controlled polymers such as DNA and proteins over its long history [...]. ...

    journal_title:International journal of molecular sciences

    pub_type: 社论

    doi:10.3390/ijms21093276

    authors: Sugawara-Narutaki A,Kamiya Y

    更新日期:2020-05-06 00:00:00

  • Every Coin Has Two Sides: Reactive Oxygen Species during Rice⁻Magnaporthe oryzae Interaction.

    abstract::Reactive oxygen species (ROS) are involved in many important processes, including the growth, development, and responses to the environments, in rice (Oryza sativa) and Magnaporthe oryzae. Although ROS are known to be critical components in rice⁻M. oryzae interactions, their regulations and pathways have not yet been ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20051191

    authors: Kou Y,Qiu J,Tao Z

    更新日期:2019-03-08 00:00:00

  • The Autophagy Status of Cancer Stem Cells in Gliobastoma Multiforme: From Cancer Promotion to Therapeutic Strategies.

    abstract::Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor featuring rapid cell proliferation, treatment resistance, and tumor relapse. This is largely due to the coexistence of heterogeneous tumor cell populations with different grades of differentiation, and in particular, to a small subset ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20153824

    authors: Ryskalin L,Gaglione A,Limanaqi F,Biagioni F,Familiari P,Frati A,Esposito V,Fornai F

    更新日期:2019-08-05 00:00:00

  • Aquaporins in Health and Disease: An Overview Focusing on the Gut of Different Species.

    abstract::Aquaporins (AQPs) play a pivotal role in gut homeostasis since their distribution and function is modulated both in physiological and in pathophysiological conditions. The transport of water and solutes through gut epithelia is essential for osmoregulation and digestive and absorptive functions. This passage is regula...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms17081213

    authors: Pelagalli A,Squillacioti C,Mirabella N,Meli R

    更新日期:2016-07-27 00:00:00

  • Mitogen-Activated Protein Kinase Phosphatases (MKPs) in Fungal Signaling: Conservation, Function, and Regulation.

    abstract::Mitogen-activated protein kinases (MAPKs) are key mediators of signaling in fungi, participating in the response to diverse stresses and in developmental processes. Since the precise regulation of MAPKs is fundamental for cell physiology, fungi bear dual specificity phosphatases (DUSPs) that act as MAP kinase phosphat...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20071709

    authors: González-Rubio G,Fernández-Acero T,Martín H,Molina M

    更新日期:2019-04-05 00:00:00

  • DPIE [2-(1,2-diphenyl-1H-indol-3-yl)ethanamine] Augments Pro-Inflammatory Cytokine Production in IL-1β-Stimulated Primary Human Oral Cells.

    abstract::Interleukin-1β (IL-1β) is a prominent pro-inflammatory cytokine that is implicated in a variety of autoimmune diseases and plays an important role in host defense against infections. IL-1β activity increases with its increasing binding capacity to IL-1 receptors (IL-1Rs). Thus, numerous studies have tar...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms19071835

    authors: Ahn SH,Lee JK,Kim ND,Kim SH,Lee S,Jung S,Chay KO,Lee TH

    更新日期:2018-06-22 00:00:00

  • Wisteria floribunda Agglutinin-Positive Mac-2 Binding Protein as a Screening Tool for Significant Liver Fibrosis in Health Checkup.

    abstract::Chronic liver disease is generally widespread, and a test for screening fibrotic subjects in a large population is needed. The ability of Wisteria floribunda agglutinin-positive mac-2 binding protein (WFA+-M2BP) to detect significant fibrosis was investigated in health checkup subjects in this research. Of 2021 health...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms22010040

    authors: Tamaki N,Kurosaki M,Takahashi Y,Itakura Y,Kirino S,Inada K,Yamashita K,Sekiguchi S,Hayakawa Y,Osawa L,Higuchi M,Takaura K,Maeyashiki C,Kaneko S,Yasui Y,Tsuchiya K,Nakanishi H,Itakura J,Loomba R,Izumi N

    更新日期:2020-12-22 00:00:00

  • TFIP11 interacts with mDEAH9, an RNA helicase involved in spliceosome disassembly.

    abstract::Yeast proteins Ntr1, Ntr2 and Prp43 function in spliceosome disassembly. An Ntr1-Ntr2 protein complex recruits Prp43 to allow the removal of the lariat-intron in late-stage RNA splicing activity. Based on amino-acid sequence similarities across species, TFIP11 and mDEAH9/Dhx15 have been identified as homologues of yea...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms9112105

    authors: Wen X,Tannukit S,Paine ML

    更新日期:2008-11-01 00:00:00

  • Rack1 Mediates the Interaction of P-Glycoprotein with Anxa2 and Regulates Migration and Invasion of Multidrug-Resistant Breast Cancer Cells.

    abstract::The emergence of multidrug resistance is always associated with more rapid tumor recurrence and metastasis. P-glycoprotein (P-gp), which is a well-known multidrug-efflux transporter, confers enhanced invasion ability in drug-resistant cells. Previous studies have shown that P-gp probably exerts its tumor-promoting fun...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms17101718

    authors: Yang Y,Wu N,Wang Z,Zhang F,Tian R,Ji W,Ren X,Niu R

    更新日期:2016-10-13 00:00:00

  • Vitamin D's Effect on the Proliferation and Inflammation of Human Intervertebral Disc Cells in Relation to the Functional Vitamin D Receptor Gene FokI Polymorphism.

    abstract::Vitamin D is known to have immunomodulatory effects, is involved in osteo-cartilaginous metabolism, and may have a role in human intervertebral disc pathophysiology. Although a link between vitamin D receptor (VDR) gene variants and disc degeneration-related pathologies has been observed, its functional contribution t...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms19072002

    authors: De Luca P,de Girolamo L,Perucca Orfei C,Viganò M,Cecchinato R,Brayda-Bruno M,Colombini A

    更新日期:2018-07-09 00:00:00

  • In Search for the Membrane Regulators of Archaea.

    abstract::Membrane regulators such as sterols and hopanoids play a major role in the physiological and physicochemical adaptation of the different plasmic membranes in Eukarya and Bacteria. They are key to the functionalization and the spatialization of the membrane, and therefore indispensable for the cell cycle. No archaeon h...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20184434

    authors: Salvador-Castell M,Tourte M,Oger PM

    更新日期:2019-09-09 00:00:00

  • Using a novel microRNA delivery system to inhibit osteoclastogenesis.

    abstract::Previously, we developed a novel microRNA (miRNA) delivery system based on bacteriophage MS2 virus-like particles (MS2 VLPs). In this current study, we used this system to transport miR-146a into human peripheral blood mononuclear cells (PBMCs), and demonstrated the inhibition of osteoclastogenesis in precursors. Two ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms16048337

    authors: Yao Y,Jia T,Pan Y,Gou H,Li Y,Sun Y,Zhang R,Zhang K,Lin G,Xie J,Li J,Wang L

    更新日期:2015-04-14 00:00:00

  • Maternal Overweight Downregulates MME (Neprilysin) in Feto-Placental Endothelial Cells and in Cord Blood.

    abstract::Maternal overweight in pregnancy alters the metabolic environment and generates chronic low-grade inflammation. This affects fetal development and programs the offspring's health for developing cardiovascular and metabolic disease later in life. MME (membrane-metalloendopeptidase, neprilysin) cleaves various peptides ...

    journal_title:International journal of molecular sciences

    pub_type: 临床试验,杂志文章

    doi:10.3390/ijms21030834

    authors: Weiß E,Berger HM,Brandl WT,Strutz J,Hirschmugl B,Simovic V,Tam-Ammersdorfer C,Cvitic S,Hiden U

    更新日期:2020-01-28 00:00:00

  • Endometrial Stromal Cells Circulate in the Bloodstream of Women with Endometriosis: A Pilot Study.

    abstract::Endometriosis is characterized by the presence of endometrial tissue outside the uterus. While endometriotic tissue is commonly localized in the pelvic cavity, it can also be found in distant sites, including the brain. The origin and pathophysiology of tissue migration is poorly understood; retrograde menstruation is...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20153740

    authors: Vallvé-Juanico J,López-Gil C,Ballesteros A,Santamaria X

    更新日期:2019-07-31 00:00:00

  • Genetic diversity and population structure of Siberian apricot (Prunus sibirica L.) in China.

    abstract::The genetic diversity and population genetic structure of 252 accessions from 21 Prunus sibirica L. populations were investigated using 10 ISSR, SSR, and SRAP markers. The results suggest that the entire population has a relatively high level of genetic diversity, with populations HR and MY showing very high diversity...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms15010377

    authors: Li M,Zhao Z,Miao X,Zhou J

    更新日期:2013-12-31 00:00:00

  • ERα Mediates Estrogen-Induced Expression of the Breast Cancer Metastasis Suppressor Gene BRMS1.

    abstract::Recently, estrogen has been reported as putatively inhibiting cancer cell invasion and motility. This information is in direct contrast to the paradigm of estrogen as a tumor promoter. However, data suggests that the effects of estrogen are modulated by the receptor isoform with which it interacts. In order to gain a ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms17020158

    authors: Ma H,Gollahon LS

    更新日期:2016-01-26 00:00:00

  • Possible Phenotypic Consequences of Structural Differences in Idic(15) in a Small Cohort of Patients.

    abstract::Among human supernumerary marker chromosomes, the occurrence of isodicentric form of 15 origin is relatively well known due to its high frequency, both in terms of gene content and associated clinical symptoms. The associated epilepsy and autism are typically more severe than in cases with interstitial 15q duplication...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20194935

    authors: Czakó M,Till Á,Szabó A,Ripszám R,Melegh B,Hadzsiev K

    更新日期:2019-10-05 00:00:00

  • Autocrine and Paracrine Mechanisms Promoting Chemoresistance in Cholangiocarcinoma.

    abstract::Resistance to conventional chemotherapeutic agents, a typical feature of cholangiocarcinoma, prevents the efficacy of the therapeutic arsenal usually used to combat malignancy in humans. Mechanisms of chemoresistance by neoplastic cholangiocytes include evasion of drug-induced apoptosis mediated by autocrine and parac...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms18010149

    authors: Cadamuro M,Brivio S,Spirli C,Joplin RE,Strazzabosco M,Fabris L

    更新日期:2017-01-13 00:00:00

  • Clinical Considerations for a Family with Dilated Cardiomyopathy, Sudden Cardiac Death, and a Novel TTN Frameshift Mutation.

    abstract::Dilated cardiomyopathy (DCM) is the leading indication for heart transplantation. TTN gene truncating mutations account for about 25% of familial DCM cases and for 18% of sporadic DCM cases. The clinical relevance of specific variants in TTN has been difficult to determine because of the sheer size of the protein for ...

    journal_title:International journal of molecular sciences

    pub_type:

    doi:10.3390/ijms22020670

    authors: Micaglio E,Monasky MM,Bernardini A,Mecarocci V,Borrelli V,Ciconte G,Locati ET,Piccoli M,Ghiroldi A,Anastasia L,Pappone C

    更新日期:2021-01-12 00:00:00

  • A Hybrid Deep Learning Model for Predicting Protein Hydroxylation Sites.

    abstract::Protein hydroxylation is one type of post-translational modifications (PTMs) playing critical roles in human diseases. It is known that protein sequence contains many uncharacterized residues of proline and lysine. The question that needs to be answered is: which residue can be hydroxylated, and which one cannot. The ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms19092817

    authors: Long H,Liao B,Xu X,Yang J

    更新日期:2018-09-18 00:00:00

  • Role of Inflammation in Diabetic Retinopathy.

    abstract::Diabetic retinopathy is a common complication of diabetes and remains the leading cause of blindness among the working-age population. For decades, diabetic retinopathy was considered only a microvascular complication, but the retinal microvasculature is intimately associated with and governed by neurons and glia, whi...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms19040942

    authors: Rübsam A,Parikh S,Fort PE

    更新日期:2018-03-22 00:00:00

  • Genetic Overlap between General Cognitive Function and Schizophrenia: A Review of Cognitive GWASs.

    abstract::General cognitive (intelligence) function is substantially heritable, and is a major determinant of economic and health-related life outcomes. Cognitive impairments and intelligence decline are core features of schizophrenia which are evident before the onset of the illness. Genetic overlaps between cognitive impairme...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms19123822

    authors: Ohi K,Sumiyoshi C,Fujino H,Yasuda Y,Yamamori H,Fujimoto M,Shiino T,Sumiyoshi T,Hashimoto R

    更新日期:2018-11-30 00:00:00

  • The Impact of the 'Mis-Peptidome' on HLA Class I-Mediated Diseases: Contribution of ERAP1 and ERAP2 and Effects on the Immune Response.

    abstract::The strong association with the Major Histocompatibility Complex (MHC) class I genes represents a shared trait for a group of autoimmune/autoinflammatory disorders having in common immunopathogenetic basis as well as clinical features. Accordingly, the main risk factors for Ankylosing Spondylitis (AS), prototype of th...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21249608

    authors: Tedeschi V,Paldino G,Paladini F,Mattorre B,Tuosto L,Sorrentino R,Fiorillo MT

    更新日期:2020-12-17 00:00:00

  • Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus Bordetella Analyzed Exclusively by NMR Spectroscopy.

    abstract::Bacterial pathogens expose on the cell surface a variety of complex carbohydrate molecules. Gram-negative bacteria produce lipopolysaccharides, which are the main components of the outer membrane of bacterial envelopes and play a major role in host-pathogen interactions. B. pertussis, B. parapertussis, B. bronchisepti...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms22031029

    authors: Ucieklak K,Koj S,Niedziela T

    更新日期:2021-01-21 00:00:00

  • Innovative Strategy for 3D Transfection of Primary Human Stem Cells with BMP-2 Expressing Plasmid DNA: A Clinically Translatable Strategy for Ex Vivo Gene Therapy.

    abstract::Ex vivo gene therapy offers enormous potential for cell-based therapies, however, cumbersome in vitro cell culture conditions have limited its use in clinical practice. We have optimized an innovative strategy for the transient transfection of bone morphogenetic protein-2 (BMP-2) expressing plasmids in suspended human...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20010056

    authors: Paidikondala M,Kadekar S,Varghese OP

    更新日期:2018-12-23 00:00:00

  • Multiple Links between HD-Zip Proteins and Hormone Networks.

    abstract::HD-Zip proteins are unique to plants, and contain a homeodomain closely linked to a leucine zipper motif, which are involved in dimerization and DNA binding. Based on homology in the HD-Zip domain, gene structure and the presence of additional motifs, HD-Zips are divided into four families, HD-Zip I⁻IV. Phylogenetic a...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms19124047

    authors: Sessa G,Carabelli M,Possenti M,Morelli G,Ruberti I

    更新日期:2018-12-14 00:00:00