当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Probing fibronectin adsorption on chemically defined surfaces by means of single molecule force microscopy.

Probing fibronectin adsorption on chemically defined surfaces by means of single molecule force microscopy.

Abstract：

:Atomic force microscope (AFM) based single molecule force spectroscopy (SMFS) and a quartz crystal microbalance (QCM) were respectively employed to probe interfacial characteristics of fibronectin fragment FNIII8-14 and full-length fibronectin (FN) on CH3-, OH-, COOH-, and NH2-terminated alkane-thiol self-assembled monolayers (SAMs). Force-distance curves acquired between hexahistidine-tagged FNIII8-14 immobilised on trisNTA-Ni2+ functionalized AFM cantilevers and the OH and COOH SAM surfaces were predominantly 'loop-like' (76% and 94% respectively), suggesting domain unfolding and preference for 'end-on' oriented binding, while those generated with NH2 and CH3 SAMs were largely 'mixed type' (81% and 86%, respectively) commensurate with unravelling and desorption, and 'side-on' binding. Time-dependent binding of FN to SAM-coated QCM crystals occurred in at least two phases: initial rapid coverage over the first 5 min; and variably diminishing adsorption thereafter (5-70 min). Loading profiles and the final hydrated surface concentrations reached (~ 950, ~ 1200, ~ 1400, ~ 1500 ng cm-2 for CH3, OH, COOH and NH2 SAMs) were consistent with: space-filling 'side-on' orientation and unfolding on CH3 SAM; greater numbers of FN molecules arranged 'end-on' on OH and especially COOH SAMs; and initial 'side-on' contact, followed by either (1) gradual tilting to a space-saving 'end-on' configuration, or (2) bi-/multi-layer adsorption on NH2 SAM.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Liamas E,Black RA,Mulheran PA,Tampé R,Wieneke R,Thomas ORT,Zhang ZJ

doi

10.1038/s41598-020-72617-z

subject

Has Abstract

pub_date

2020-09-24 00:00:00

pages

15662

issue

1

issn

2045-2322

pii

10.1038/s41598-020-72617-z

journal_volume

10

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Evidence for genetic association of TBX21 and IFNG with systemic lupus erythematosus in a Chinese Han population.

    abstract：:TBX21 recode T-bet which is an important transcription factor that drives the Th1 immune response primarily by promoting expression of the interferon-gamma (IFNG) gene. Recent studies have shown that genetic variants in TBX21 and IFNG are connected with risk of systemic lupus erythematosus (SLE). The aim of the presen...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep22081

    authors： Leng RX,Pan HF,Liu J,Yang XK,Zhang C,Tao SS,Wang DG,Li XM,Li XP,Yang W,Ye DQ

    更新日期：2016-02-26 00:00:00

  • Differential cohesin loading marks paired and unpaired regions of platypus sex chromosomes at prophase I.

    abstract：:Cohesins are vital for chromosome organisation during meiosis and mitosis. In addition to the important function in sister chromatid cohesion, these complexes play key roles in meiotic recombination, DSB repair, homologous chromosome pairing and segregation. Egg-laying mammals (monotremes) feature an unusually complex...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-04560-5

    authors： Casey AE,Daish TJ,Barbero JL,Grützner F

    更新日期：2017-06-26 00:00:00

  • Constructing Smaller Pauli Twirling Sets for Arbitrary Error Channels.

    abstract：:Twirling is a technique widely used for converting arbitrary noise channels into Pauli channels in error threshold estimations of quantum error correction codes. It is vitally useful both in real experiments and in classical quantum simulations. Minimising the size of the twirling gate set increases the efficiency of ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-46722-7

    authors： Cai Z,Benjamin SC

    更新日期：2019-08-02 00:00:00

  • Psychological distress and lack of PINK1 promote bioenergetics alterations in peripheral blood mononuclear cells.

    abstract：:Psychological distress induces oxidative stress and alters mitochondrial metabolism in the nervous and immune systems. Psychological distress promotes alterations in brain metabolism and neurochemistry in wild-type (WT) rats in a similar manner as in Parkinsonian rats lacking endogenous PTEN-induced kinase 1 (PINK1), ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-66745-9

    authors： Grigoruţă M,Dagda RK,Díaz-Sánchez ÁG,Martínez-Martínez A

    更新日期：2020-06-17 00:00:00

  • Ethylene and hydrogen peroxide are involved in brassinosteroid-induced salt tolerance in tomato.

    abstract：:Crosstalk between phytohormone pathways is essential in plant growth, development and stress responses. Brassinosteroids (BRs) and ethylene are both pivotal plant growth regulators, and the interaction between these two phytohormones in the tomato response to salt stress is still unclear. Here, we explored the mechani...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep35392

    authors： Zhu T,Deng X,Zhou X,Zhu L,Zou L,Li P,Zhang D,Lin H

    更新日期：2016-10-14 00:00:00

  • Evaluation of candidate reference genes for RT-qPCR studies in three metabolism related tissues of mice after caloric restriction.

    abstract：:Reverse transcription quantitative-polymerase chain reaction (RT-qPCR) is a routine method for gene expression analysis, and reliable results depend on proper normalization by stable reference genes. Caloric restriction (CR) is a robust lifestyle intervention to slow aging and delay onset of age-associated diseases vi...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep38513

    authors： Gong H,Sun L,Chen B,Han Y,Pang J,Wu W,Qi R,Zhang TM

    更新日期：2016-12-06 00:00:00

  • SERPINB3 is associated with longer survival in transgenic mice.

    abstract：:The physiological roles of the protease inhibitor SERPINB3 (SB3) are still largely unknown. The study was addressed to assess the biological effects of this serpin in vivo using a SB3 transgenic mouse model. Two colonies of mice (123 transgenic for SB3 and 148 C57BL/6J controls) have been studied. Transgenic (TG) mice...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep03056

    authors： Villano G,Ruvoletto M,Ceolotto G,Quarta S,Calabrese F,Turato C,Tono N,Crescenzi M,Biasiolo A,Cattelan A,Merkel C,Avogaro A,Gatta A,Pontisso P

    更新日期：2013-10-28 00:00:00

  • Long-term in vivo imaging reveals tumor-specific dissemination and captures host tumor interaction in zebrafish xenografts.

    abstract：:Understanding mechanisms mediating tumor metastasis is crucial for diagnostic and therapeutic targeting. Here, we take advantage of a transparent embryonic zebrafish xenograft model (eZXM) to visualize and track metastatic cells in real time using selective plane illumination microscopy (SPIM) for up to 30 h. Injected...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-69956-2

    authors： Asokan N,Daetwyler S,Bernas SN,Schmied C,Vogler S,Lambert K,Wobus M,Wermke M,Kempermann G,Huisken J,Brand M,Bornhäuser M

    更新日期：2020-08-06 00:00:00

  • Delayed recruiting of TPD52 to lipid droplets - evidence for a "second wave" of lipid droplet-associated proteins that respond to altered lipid storage induced by Brefeldin A treatment.

    abstract：:Tumor protein D52 (TPD52) is amplified and overexpressed in breast and prostate cancers which are frequently characterised by dysregulated lipid storage and metabolism. TPD52 expression increases lipid storage in mouse 3T3 fibroblasts, and co-distributes with the Golgi marker GM130 and lipid droplets (LDs). We examine...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-46156-1

    authors： Chen Y,Frost S,Khushi M,Cantrill LC,Yu H,Arthur JW,Bright RK,Groblewski GE,Byrne JA

    更新日期：2019-07-05 00:00:00

  • Systematic discovery about NIR spectral assignment from chemical structural property to natural chemical compounds.

    abstract：:Spectra-structure interrelationship is still the weakness of NIR spectral assignment. In this paper, a comprehensive investigation from chemical structural property to natural chemical compounds was carried out for NIR spectral assignment. Surprisingly, we discovered that NIR absorption frequency of the skeleton struc...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-45945-y

    authors： Ma L,Peng Y,Pei Y,Zeng J,Shen H,Cao J,Qiao Y,Wu Z

    更新日期：2019-07-01 00:00:00

  • Grammatical category and the neural processing of phrases.

    abstract：:The interlocking roles of lexical, syntactic and semantic processing in language comprehension has been the subject of longstanding debate. Recently, the cortical response to a frequency-tagged linguistic stimulus has been shown to track the rate of phrase and sentence, as well as syllable, presentation. This could be...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-021-81901-5

    authors： Burroughs A,Kazanina N,Houghton C

    更新日期：2021-01-28 00:00:00

  • Identification of DKK-1 as a novel mediator of statin effects in human endothelial cells.

    abstract：:This study shows that DKK-1, a member of the Dickkopf family and a regulator of the Wnt pathways, represents a novel target of statins which, through the inhibition of HMG-CoA reductase and of non-steroidal isoprenoid intermediates, exert extra-beneficial effect in preventing atherosclerosis beyond their effect on the...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-35119-7

    authors： Pontremoli M,Brioschi M,Baetta R,Ghilardi S,Banfi C

    更新日期：2018-11-12 00:00:00

  • Imaging and radiation effects of gold nanoparticles in tumour cells.

    abstract：:Gold nanoparticle radiosensitization represents a novel technique in enhancement of ionising radiation dose and its effect on biological systems. Variation between theoretical predictions and experimental measurement is significant enough that the mechanism leading to an increase in cell killing and DNA damage is stil...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep19442

    authors： McQuaid HN,Muir MF,Taggart LE,McMahon SJ,Coulter JA,Hyland WB,Jain S,Butterworth KT,Schettino G,Prise KM,Hirst DG,Botchway SW,Currell FJ

    更新日期：2016-01-20 00:00:00

  • Opposing regulation of the late phase TNF response by mTORC1-IL-10 signaling and hypoxia in human macrophages.

    abstract：:Tumor necrosis factor (TNF) is best known for inducing a rapid but transient NF-κB-mediated inflammatory response. We investigated later phases of TNF signaling, after the initial transient induction of inflammatory genes has subsided, in primary human macrophages. TNF signaling induced expression of late response gen...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep31959

    authors： Huynh L,Kusnadi A,Park SH,Murata K,Park-Min KH,Ivashkiv LB

    更新日期：2016-08-25 00:00:00

  • Dynamic interplay of multidrug transporters with TolC for isoprenol tolerance in Escherichia coli.

    abstract：:Engineering of efflux pumps is a promising way to improve host's tolerance to biofuels such as medium-chain alcohols (CmOHs); however, this strategy is restricted by poor understanding of the efflux pumps engaged in extrusion of solvents. In this study, several Escherichia coli mutants of multidrug transporters were e...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep16505

    authors： Wang C,Yang L,Shah AA,Choi ES,Kim SW

    更新日期：2015-11-13 00:00:00

  • Zeno dynamics in quantum open systems.

    abstract：:Quantum Zeno effect shows that frequent observations can slow down or even stop the unitary time evolution of an unstable quantum system. This effect can also be regarded as a physical consequence of the statistical indistinguishability of neighboring quantum states. The accessibility of quantum Zeno dynamics under un...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep11509

    authors： Zhang YR,Fan H

    更新日期：2015-06-23 00:00:00

  • Treatment of T1D via optimized expansion of antigen-specific Tregs induced by IL-2/anti-IL-2 monoclonal antibody complexes and peptide/MHC tetramers.

    abstract：:Type 1 diabetes can be overcome by regulatory T cells (Treg) in NOD mice yet an efficient method to generate and maintain antigen-specific Treg is difficult to come by. Here, we devised a combination therapy of peptide/MHC tetramers and IL-2/anti-IL-2 monoclonal antibody complexes to generate antigen-specific Treg and...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-26161-6

    authors： Izquierdo C,Ortiz AZ,Presa M,Malo S,Montoya A,Garabatos N,Mora C,Verdaguer J,Stratmann T

    更新日期：2018-05-25 00:00:00

  • Tobacco smoke and morphine alter peripheral and CNS inflammation following HIV infection in a humanized mouse model.

    abstract：:Tobacco smoking is common in HIV-infected patients, and is prevalent among intravenous opiate abusers. Conversely, intravenous opiate abusers are more likely HIV-infected, and opiate abuse is associated with more severe neuroinflammation. Given the coincident use of tobacco smoking among HIV-infected intravenous drug ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-70374-7

    authors： Cornwell WD,Sriram U,Seliga A,Zuluaga-Ramirez V,Gajghate S,Rom S,Winfield M,Heldt NA,Ambrose D,Rogers TJ,Persidsky Y

    更新日期：2020-08-19 00:00:00

  • The Fall of Sleep K-Complex in Alzheimer Disease.

    abstract：:Although a slowing of electroencephalographic (EEG) activity during wakefulness and -to some extent- sleep of Alzheimer disease (AD) patients (i.e., increased slow-frequency activity) was documented, recent findings in healthy elderly show a decreased 0.6-1 Hz slow wave activity (SWA) during NREM, which was associated...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep39688

    authors： De Gennaro L,Gorgoni M,Reda F,Lauri G,Truglia I,Cordone S,Scarpelli S,Mangiaruga A,D'atri A,Lacidogna G,Ferrara M,Marra C,Rossini PM

    更新日期：2017-01-03 00:00:00

  • Ecosystem regime change inferred from the distribution of trace metals in Lake Erie sediments.

    abstract：:Many freshwater and coastal marine ecosystems across the world may have undergone an ecosystem regime change due to a combination of rising anthropogenic disturbances and regional climate change. Such a change in aquatic ecosystems is commonly seen as shifts in algal species. But considerably less detail is known abou...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep07265

    authors： Yuan F,Depew R,Soltis-Muth C

    更新日期：2014-12-01 00:00:00

  • Carbon Quantum Dots for Zebrafish Fluorescence Imaging.

    abstract：:Carbon quantum dots (C-QDs) are becoming a desirable alternative to metal-based QDs and dye probes owing to their high biocompatibility, low toxicity, ease of preparation, and unique photophysical properties. Herein, we describe fluorescence bioimaging of zebrafish using C-QDs as probe in terms of the preparation of C...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep11835

    authors： Kang YF,Li YH,Fang YW,Xu Y,Wei XM,Yin XB

    更新日期：2015-07-02 00:00:00

  • Flexible and Accurate Simulation of Radiation Cooling with FETD Method.

    abstract：:Thermal management and simulation are becoming increasingly important in many areas of engineering applications. There are three cooling routes for thermal management, namely thermal conduction, thermal convection and thermal radiation, among which the first two approaches have been widely studied and applied, while t...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-21020-w

    authors： Zhang HH,Sha WEI,Huang ZX,Shi GM

    更新日期：2018-02-08 00:00:00

  • A threshold of endogenous stress is required to engage cellular response to protect against mutagenesis.

    abstract：:Endogenous stress represents a major source of genome instability, but is in essence difficult to apprehend. Incorporation of labeled radionuclides into DNA constitutes a tractable model to analyze cellular responses to endogenous attacks. Here we show that incorporation of [(3)H]thymidine into CHO cells generates oxi...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep29412

    authors： Saintigny Y,Chevalier F,Bravard A,Dardillac E,Laurent D,Hem S,Dépagne J,Radicella JP,Lopez BS

    更新日期：2016-07-11 00:00:00

  • Mycobacterium tuberculosis peptide E7/HLA-DRB1 tetramers with different HLA-DR alleles bound CD4+ T cells might share identical CDR3 region.

    abstract：:Human CD4+ T cells play an important role in the immune response to Mycobacterium tuberculosis (MTB). However, little is known about the spectratyping characteristics of the CD4+ T-cell receptor (TCR) α- and β-chains CDR3 region in tuberculosis (TB) patients. We sorted MTB peptide E7-bound CD4+ T cells by using E7/HLA...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-28344-7

    authors： Gan Y,Wang C,Fang Y,Yao Y,Tu X,Wang J,Huang X,Tan Y,Chen T,Zhang K,Shen Y,Zhou L,Liu J,Lai X

    更新日期：2018-07-02 00:00:00

  • ABCG2 transporter inhibitor restores the sensitivity of triple negative breast cancer cells to aminolevulinic acid-mediated photodynamic therapy.

    abstract：:Photosensitizer protoporphyrin IX (PpIX) fluorescence, intracellular localization and cell response to photodynamic therapy (PDT) were analyzed in MCF10A normal breast epithelial cells and a panel of human breast cancer cells including estrogen receptor (ER) positive, human epidermal growth factor receptor 2 (HER2) po...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep13298

    authors： Palasuberniam P,Yang X,Kraus D,Jones P,Myers KA,Chen B

    更新日期：2015-08-18 00:00:00

  • Elevated ubiquitinated proteins in brain and blood of individuals with schizophrenia.

    abstract：:Dysregulation of the ubiquitin proteasome system (UPS) has been linked to schizophrenia but it is not clear if this dysregulation is detectable in both brain and blood. We examined free mono-ubiquitin, ubiquitinated proteins, catalytic ubiquitination, and proteasome activities in frozen postmortem OFC tissue from 76 (...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-38490-1

    authors： Bousman CA,Luza S,Mancuso SG,Kang D,Opazo CM,Mostaid MS,Cropley V,McGorry P,Shannon Weickert C,Pantelis C,Bush AI,Everall IP

    更新日期：2019-02-19 00:00:00

  • Effects of Obesity Related Genetic Variations on Visceral and Subcutaneous Fat Distribution in a Chinese Population.

    abstract：:Genome-wide association studies (GWAS) have uncovered numerous variants associated with body mass index (BMI), waist circumference, and waist-to-hip ratio. Our study aims to investigate how these variants are linked to fat distribution. We genotyped 56 validated variants of BMI, waist circumference, and waist-to-hip r...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep20691

    authors： Wang T,Ma X,Peng D,Zhang R,Sun X,Chen M,Yan J,Wang S,Yan D,He Z,Jiang F,Bao Y,Hu C,Jia W

    更新日期：2016-02-05 00:00:00

  • Sex-biased patterns shaped the genetic history of Roma.

    abstract：:The Roma population is a European ethnic minority characterized by recent and multiple dispersals and founder effects. After their origin in South Asia around 1,500 years ago, they migrated West. In Europe, they diverged into ethnolinguistically distinct migrant groups that spread across the continent. Previous geneti...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-71066-y

    authors： García-Fernández C,Font-Porterias N,Kučinskas V,Sukarova-Stefanovska E,Pamjav H,Makukh H,Dobon B,Bertranpetit J,Netea MG,Calafell F,Comas D

    更新日期：2020-09-02 00:00:00

  • A transgenic mouse model expressing an ERα folding biosensor reveals the effects of Bisphenol A on estrogen receptor signaling.

    abstract：:Estrogen receptor-α (ERα) plays an important role in normal and abnormal physiology of the human reproductive system by interacting with the endogenous ligand estradiol (E2). However, other ligands, either analogous or dissimilar to E2, also bind to ERα. This may create unintentional activation of ER signaling in repr...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep34788

    authors： Sekar TV,Foygel K,Massoud TF,Gambhir SS,Paulmurugan R

    更新日期：2016-10-10 00:00:00

  • A new primitive Neornithischian dinosaur from the Jurassic of Patagonia with gut contents.

    abstract：:We describe a new species of an ornithischian dinosaur, Isaberrysaura mollensis gen. et sp. nov. The specimen, consisting in an almost complete skull and incomplete postcranium was collected from the marine-deltaic deposits of the Los Molles Formation (Toarcian-Bajocian), being the first reported dinosaur for this uni...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep42778

    authors： Salgado L,Canudo JI,Garrido AC,Moreno-Azanza M,Martínez LC,Coria RA,Gasca JM

    更新日期：2017-02-16 00:00:00